Advanced Spectroscopic and Calorimetric Techniques for Pharmaceutical Analysis

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Pharmaceutical Processes".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 561

Special Issue Editor


E-Mail Website
Guest Editor
Department of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 40-055 Katowice, Poland
Interests: glycation; serum albumin; protein-ligand interactions; binding properties; combination therapy; structural protein modifications; fatty acids; spectroscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The interactions between pharmaceutical compounds and proteins are foundational to biological sciences. The study of drug–protein interactions, particularly involving serum proteins, is among the most exciting areas in contemporary science, employing various spectroscopic and calorimetric techniques to analyze their structure–function relationships due to the complex physicochemical properties of the substances involved. Understanding these interactions is essential for developing a fundamental knowledge base related to molecular-level behaviours.

Advanced spectroscopic techniques offer detailed insights into the molecular structures and binding properties of pharmaceutical compounds. Techniques such as Nuclear Magnetic Resonance (NMR), Circular Dichroism (CD), and Ultraviolet–Visible (UV-Vis) and Fluorescence Spectroscopy (SFM) provide essential information on binding sites, conformational changes, and the dynamics of these processes. Understanding these aspects is vital for advancing drug design and ensuring the efficacy and safety of pharmaceutical products.

Calorimetric techniques provide valuable thermodynamic data on drug–protein interactions by measuring the heat changes associated with binding processes. Methods such as Isothermal Titration Calorimetry (ITC) and Differential Scanning Calorimetry (DSC) complement spectroscopic techniques by offering insights into the enthalpic and entropic contributions to these interactions. These data are crucial for optimizing drug efficacy and stability, helping us understand the energy changes which occur during drug–protein binding and contributing to the overall design and improvement of pharmaceutical compounds.

The Special Issue will include, but not be limited to, the following topics:

  • Application of CD, UV-Vis, NMR, and Fluorescence Spectroscopy in studying drug–protein interactions;
  • Thermodynamic analysis of drug binding using ITC and DSC;
  • Structural and functional characterization of pharmaceutical compounds;
  • Integration of spectroscopic and calorimetric data for a comprehensive analysis of ligand–protein interactions;
  • Case studies demonstrating practical applications in drug discovery and development.

This Special Issue aims to provide a platform for publishing research on ligand–protein interactions using multidisciplinary techniques, particularly relevant for applications in the pharmaceutical and biomedical fields. I look forward to your valuable contributions to this exciting area of research.

Dr. Agnieszka Szkudlarek
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • pharmaceutical applications
  • ligand–protein interactions
  • transport proteins
  • protein modifications
  • spectroscopic and calorimetric techniques
  • protein structure and function
  • drug efficacy and stability
  • pharmaceutical compounds
  • exogenous and endogenous ligands
  • biomedical research

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

19 pages, 1391 KiB  
Article
New TLC-Densitometric Method for the Quantification of Donepezil in Tablets
by Wioletta Parys and Alina Pyka-Pająk
Processes 2025, 13(4), 1106; https://doi.org/10.3390/pr13041106 - 7 Apr 2025
Viewed by 268
Abstract
A new TLC method combined with densitometry was developed for the determination of donepezil hydrochloride in Cogiton Biofarm and Donecept Actavis tablets. The analyses were performed on TLC silica gel 60F254 plates with mobile phase of n-butanol + n-propanol + [...] Read more.
A new TLC method combined with densitometry was developed for the determination of donepezil hydrochloride in Cogiton Biofarm and Donecept Actavis tablets. The analyses were performed on TLC silica gel 60F254 plates with mobile phase of n-butanol + n-propanol + acetone + water + glacial acetic acid at ratio of 2:2:1:1:1, v/v. The proposed mobile phase is miscible and after development the chromatographic plate has a homogeneous background in visible light. Densitometric analysis at λ = 319 nm was used for quantitative studies. The method was linear from 1.0 to 5.0 µg/spot and from 0.2 to 1.0 µg/spot and it was validated for both concentration ranges. The presented method is rapid, selective, linear, accurate, precise, robust, and economical. The results of the donepezil content in drugs calculated from both calibration curves were that no statistically significant differences were observed. The obtained content of donepezil in Cogiton (99.2%) and Donecept (99.0%) tablets is within the deviations permitted by the European Pharmacopoeia in relation to the amount declared by the manufacturer. The novelty of the study consists of the development of chromatographic conditions allowing the separation of as many as six donepezil degradation products with the simultaneous use of TLC chromatographic plates. As a result, the proposed method is economical, since it is several times cheaper than using HPTLC plates. While Ali et al. separated a maximum of three degradation products from donepezil, Pandey et al. successfully separated only two donepezil-related substances from donepezil. The proposed new TLC method combined with densitometry can be used for the routine control of donepezil in pharmaceutical preparations (tablets). Since TLC is less sensitive and precise compared to HPLC, it can be used as a complementary technique. Full article
Show Figures

Figure 1

Back to TopTop