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Biotechnology and Biochemistry of Crop Plants

A special issue of Sustainability (ISSN 2071-1050). This special issue belongs to the section "Sustainable Agriculture".

Deadline for manuscript submissions: closed (31 March 2024) | Viewed by 2623

Special Issue Editor


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Guest Editor
School of Public Health, Wolaita Sodo University, Wolaita, Ethiopia
Interests: plant physiology; nanobiotechnology; environmental botany; medicinal plants; plant propagation

Special Issue Information

Dear Colleagues,

Crop plant biotechnology and biochemistry are important disciplines in agricultural science. Whereas in biotechnological studies, techniques for achieving specific requirements are explored using plant systems, biochemical investigations examine crop plants from the cellular to whole-plant level under both normal and adverse conditions. Extreme climatic conditions due to various reasons are increasing disease occurrences, pest attacks, loss of soil fertility, soil salinity level, drought, extreme temperatures, greenhouse gases, and so on, all of which have created immediate challenges to agricultural practices around the world. Therefore, knowledge of various biotechnological and biochemical aspects of crop plants remains essential for their sustainable development under both normal and adverse conditions. Biochemical studies mainly focus on the processes of photosynthesis, respiration, nitrogen fixation, hormones, enzyme production, and so on. These features are useful to screen and evaluate the status of crop plants’ growth, production, etc. However, biotechnological studies, such as tissue culture and genetic engineering, explore ways in which crop plants can be improved. These techniques are used for the development of new crop plant traits and varieties that can cope with adverse conditions. Biotechnology has given rise to a new age of science and technology, boosting plant-based secondary metabolite production, crop plant improvement, germplasm conservation, disease-free plant production, and the development of new varieties. This Special Issue will provide a platform for researchers to share their experiences and facilitate further discussion to promote research and applications based on biotechnological and biochemical knowledge for crop improvement. This Special Issue invites original research papers, reviews, and short communications on the following areas, as well as related topics:

  1. Biochemical process;
  2. Photosynthesis;
  3. Growth hormones;
  4. Enzymes;
  5. Biotechnological studies;
  6. Genetic engineering;
  7. Transgenic crop plants;
  8. Micropropagation;
  9. Stress, signaling and defense responses;
  10. Biosynthesis and regulation of specialized metabolites.

Prof. Dr. Azamal Husen
Guest Editor

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Keywords

  • crop plant growth
  • adverse conditions
  • biochemical studies
  • crop plant biotechnology
  • tissue culture
  • transgenic crop plants

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Published Papers (1 paper)

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Research

18 pages, 7435 KiB  
Article
Synthesis and Accumulation of Phytocompounds in Field-, Tissue-Culture Grown (Stress) Root Tissues and Simultaneous Defense Response Activity in Glycyrrhiza glabra L.
by Yashika Bansal, Abdul Mujib, Jyoti Mamgain, Shubham Kumar, Yaser Hassan Dewir and Katalin Magyar-Tábori
Sustainability 2024, 16(4), 1613; https://doi.org/10.3390/su16041613 - 15 Feb 2024
Cited by 3 | Viewed by 1898
Abstract
Harsh climates, i.e., drought, extreme temperatures, and toxic gases, pose issues to agriculture by altering plants’ growth and yield. Biotechnology with biochemical defense approaches is beneficial for generating new plants/varieties with extra resilience to adverse conditions. In response to stress, cultures show an [...] Read more.
Harsh climates, i.e., drought, extreme temperatures, and toxic gases, pose issues to agriculture by altering plants’ growth and yield. Biotechnology with biochemical defense approaches is beneficial for generating new plants/varieties with extra resilience to adverse conditions. In response to stress, cultures show an enriched level of secondary metabolite synthesis. Here, an efficient in vitro propagation method using axillary shoot proliferation, along with callus formation, was established in Glycyrrhiza glabra L. The phytochemical composition of in vitro and in vivo grown tissues was analyzed using a gas chromatography–mass spectrometry (GC–MS) technique, and the biochemical attributes were measured and compared in different investigated tissues. Callus formation from root explants was achieved with a frequency of 88.89% on MS medium containing 2.0 mg/L BAP and 0.5 mg/L 2,4-D. Axillary shoot proliferation was obtained from dormant buds when cultured onto MS supplemented with BAP alone, or in combination with, IAA. The maximum shoot proliferation (94.44%) was recorded on MS with 1.0 mg/L BAP with an average shoot length of 10.5 cm. The regenerated shoots were subcultured and transferred to the root induction medium, supplemented with various concentrations of IAA/IBA, wherein 2.0 mg/L IBA resulted in the best rooting frequency (88.89%). The GC–MS-based phytocompounds analysis of the methanolic extracts of root-derived callus and in vivo- and in vitro- grown root tissues was conducted. These samples revealed the presence of more than 35 therapeutically important bioactive compounds, such as methylglabridin, sitosterol, lupeol, squalene, stearic acid, linoleic acid, etc. The biochemical parameters, like total phenolic content, flavonoid content, DPPH scavenging activity, superoxide dismutase, and peroxidase activity were also measured. All the biochemical attributes were found to be higher in in vitro derived roots than the callus and in vivo grown root (donor) samples. These findings demonstrated that callus (root derived) and in vitro roots are a stable and potent source of multiple phytocompounds, encompassing medical significance with wide applications. This study may serve as an alternative opportunity in the sustained and continuous synthesis of important compounds without harming natural vegetation and normal environment. Full article
(This article belongs to the Special Issue Biotechnology and Biochemistry of Crop Plants)
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