Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial Strain MSr12020
Abstract
:1. Introduction
2. Materials and Methods
2.1. Maintenance of Myxobacterial Cultures
2.2. Standardized HPLC–MS Conditions for Analysis of Secondary Metabolism of Crude Extracts
2.3. Isolation of 1–3 Via Liquid–Liquid Extraction, Flash Chromatography and Semi-Preparative HPLC
2.4. NMR Based Structure Elucidation and Chiroptical Measurement
2.5. Bioactivity Profiling
2.6. In Vitro Succinylation Reactions of 2 and 3
2.7. Applied Software, DNA Sequence Analysis, and Bioinformatics Methods
3. Results
3.1. Discovery, Isolation and Structural Elucidation of 1
3.2. Bioactivity of 1–3
3.3. Biosynthesis of 1–3
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zone of Inhibition in mm | |||||||
---|---|---|---|---|---|---|---|
Microorganism | 1 | 2 | 3 | Kanamycin | Fusidic Acid | Cycloheximide | MeOH/DMSO |
Escherichia coli HS 996 | 6 | 6 | 6 | 30 | NT | NT | 6 |
E. coli BW251123 | 6 | 6 | 6 | 32 | NT | NT | 6 |
Micrococcus luteus DSM 1790 | 40 | 40 | 6 | NT | 44 | NT | 6 |
Bacillus subtilis DSM 10 | 6 | 10 | 6 | NT | 27 | NT | 6 |
Mucor hiemalis DSM 2656 | 6 | 6 | 6 | NT | NT | 15 | 6 |
Pichia anomala DSM 6766 | 6 | 6 | 6 | NT | NT | 52 | 6 |
IC50 Values of 1–3 in μg/mL | ||||
---|---|---|---|---|
Cancer Cell Line | 1 | 2 | 3 | Doxorubicin |
HCT-116 | 23.3 | 23.2 | 24.3 | 0.1 |
KB-3-1 | 22.7 | 21.2 | 41.7 | 0.6 |
U-2 OS | 31.5 | 29.1 | 34.6 | 0.2 |
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Okoth, D.A.; Hug, J.J.; Garcia, R.; Müller, R. Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial Strain MSr12020. Microorganisms 2022, 10, 1959. https://doi.org/10.3390/microorganisms10101959
Okoth DA, Hug JJ, Garcia R, Müller R. Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial Strain MSr12020. Microorganisms. 2022; 10(10):1959. https://doi.org/10.3390/microorganisms10101959
Chicago/Turabian StyleOkoth, Dorothy A., Joachim J. Hug, Ronald Garcia, and Rolf Müller. 2022. "Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial Strain MSr12020" Microorganisms 10, no. 10: 1959. https://doi.org/10.3390/microorganisms10101959