Search Results (55)

Aeromonas hydrophila is a prevalent opportunistic pathogen in aquaculture. To establish a rapid, convenient, and accurate detection method for A. hydrophila, this study developed and evaluated Multi-Enzyme Isothermal Rapid Amplification (MIRA) assays, which could complete amplification within 20 min at a constant temperature of 39 °C. The basic MIRA assay targeting the aerolysin (aerA) gene demonstrated high specificity, showing no cross-reactivity with six related bacterial species including Aeromonas veronii, Vibrio harveyi, Pseudomonas fluorescens, Bacillus subtilis, Bacillus cereus, and Lactiplantibacillus plantarum. The fluorescent MIRA assay achieved a detection limit of 1 fg/μL (3.1 × 10² copies/μL) when using the pUC57-aerA standard plasmid, while real-time quantitative PCR achieved a detection limit of 0.1 fg/μL (31 copies/μL). Thus, the MIRA assay exhibited 10-fold lower sensitivity than qPCR but shortened the reaction time from several hours (nearly two hours) to within one hour. Both the specificity and sensitivity of the MIRA reactions were evaluated with three independent experiments. These findings suggested that the developed MIRA assays provide a rapid, specific, and practical diagnostic tool for A. hydrophila detection in aquaculture environments, particularly suitable for resource-limited field applications. Full article

Aeromonas hydrophila and A. dhakensis are ubiquitous microorganisms, widespread in aquatic environments, and can cause severe infections in humans and animals. This study aimed to determine the diversity of virulence genes aerA, hlyA, act, and alt through polymerase chain reaction and the antimicrobial resistance through disk diffusion test of 101 A. hydrophila and 34 A. dhakensis strains from environmental, animal, and human sources gathered between 2016 and 2019 at the National Reference Laboratory for Enteric Diseases. Overall, the virulence gene distribution was act in 35.5% of the samples, alt in 40.7%, aerA in 42.2%, and hlyA in 44.5%. Our results revealed that 76.3% of the 135 isolated Aeromonas exhibited at least one of the genes above. 76.3% of A. hydrophila and 76.5% of A. dhakensis exhibited virulence genes distributed among 15 and 12 virulence profiles, respectively. Antimicrobial resistance was observed in 86% of the strains (87.1% in A. hydrophila and 82.4% in A. dhakensis), with higher rates of resistance to Nalidixic acid (69.3%), Imipenem (31.1%), and Sulfamethoxazole-trimethoprim (15.5%). The occurrence of virulence genes and antimicrobial resistance in A. hydrophila and A. dhakensis from different sources indicates their diversity and pathogenicity, reinforcing that they can be a potential health risk source. Full article

As life expectancy increases, muscle atrophy, characterized by a decline in muscle mass and strength that can impair mobility, has become a growing concern, highlighting the potential of protein supplementation as a promising intervention strategy. A horse meat hydrolysate, with a molecular weight of less than 3 kDa, derived from m. biceps femoris and produced using the food-grade enzyme Alcalase^® (A4 < 3kDa) was evaluated for its efficacy in mitigating dexamethasone-induced muscle atrophy, a widely accepted model for studying catabolic muscle loss. Administered orally to C57BL/6 mice at dosages of 200 mg/kg or 500 mg/kg body weight for 35 days, A4 < 3kDa effectively countered the weight loss induced by dexamethasone in the whole body, quadriceps, tibialis anterior, and gastrocnemius muscles. Moreover, it increased muscle fiber cross-sectional area and grip strength. These effects were attributed to increased protein synthesis via the protein kinase B (AKT)/forkhead box O3 (FoxO3a)/mammalian target of rapamycin (mTOR) signaling pathway. A4 < 3kDa augmented the phosphorylation of key components of the signaling pathways associated with muscle atrophy, resulting in reduced mRNA expression of Atrogin-1 and MuRF-1. These findings demonstrate the potential of A4 < 3kDa as a functional food ingredient for preventing muscle atrophy. Full article

Background: Plant-based meat analogs (PBMAs) have attracted attention as alternatives to traditional meat. Methods: In this study, three beef products (BPs; BP-1, BP-2, BP-3) and three PBMA products (PPs; PP-1, PP-2, PP-3) purchased from a Korean market were evaluated for nutritional profile-based nutritional facts, such as proximate composition, total calories, and levels of mineral, cholesterol, sugar, fatty acids, and amino acids, and for sensory characteristics. Results: Cholesterol and vaccenic acid were detected only in the BP samples. The levels of crude lipids, zinc, palmitic acid, stearic acid, oleic acid, saturated fatty acids, monounsaturated fatty acids, threonine, and lysine, and the score for taste and flavor were higher for the BPs than for the PPs (p < 0.05). By contrast, the levels of carbohydrates, calcium, iron, magnesium, sodium, glucose, sucrose, total sugar, isoleucine, phenylalanine, and glutamic acid were higher for the PPs than for the BPs. The levels of protein, potassium, phosphorus, copper, aluminum, valine, leucine, histidine, and arginine did not differ significantly between the BPs and the PPs. Multivariate analysis indicated that palmitic acid influenced the differences in nutritional profiles between the BPs and the PPs. Additionally, discrepancies were observed between the measured and labeled values for total sugar in PP-1 and PP-3. Conclusions: These findings offer valuable insights for the development of processed meat products using beef and PBMAs and may help consumers make informed purchasing decisions through the provision of accurate and reliable nutritional information. Full article

Selenium-rich foods play a crucial role in human health and hold significant economic value for agricultural products. However, many regions in China are experiencing selenium deficiency, which has led to an increased demand for Se-rich agricultural products. This study focused on Nanzhang County, a key area within the “Organic Valley” of Hubei Province, China. We employed fuzzy weights-of-evidence, backpropagation neural network, and support vector regression models to predict optimal planting zones for Selenium-rich crops. A comparative analysis indicated that the backpropagation neural network model provided the highest prediction accuracy (R² = 0.77), identifying Selenium-rich crop zones covering 68.87% of the aera, where Selenium-rich crops made up 86.67% of all samples. Notably, the backpropagation neural network yielded excellent performance for rice and rapeseed, with R² values of 0.95 and 0.99, respectively. The findings also indicate that the Selenium content in crops is closely linked to Selenium levels in the soil and is significantly influenced by synergistic and antagonistic interactions with other elements. This study provides scientific support for the cultivation of selenium-rich crops. It plays a positive role in promoting the development of the local selenium-rich industry and the sustainable utilization of soil selenium resources. Full article

Aquatic products play a crucial role in meeting the increasing global demands for high-quality proteins. However, the occurrence of bacterial diseases results in significant economic losses worldwide. Aeromonas hydrophila (A. hydrophila) is the pathogen of several fish diseases. Antibiotics were widely used in combating bacterial diseases in aquaculture. The increasing occurrences of antibiotic resistance necessitate the restricted use of antibiotics. Consequently, developing drugs that avoid antibiotic resistance is important for the future of aquaculture. Quorum sensing (QS) is critical for bacterial pathogens in regulating bacterial virulence and is a promising target for developing anti-infective agents. Here, we found that turmeric oil with a MIC of 256 μg/mL could dose-dependently reduce the virulence phenotypes regulated by QS, ranging from 8 to 64 μg/mL, suggesting that sub-inhibitory concentrations of turmeric oil could inhibit bacterial virulence. Further qPCR findings demonstrated that turmeric oil could significantly inhibit the transcription of aerA, ahyI, and ahyR by a 54-fold, 36-fold, and 56-fold change reduction, respectively. Cell live/dead staining and animal study results showed that turmeric oil could inhibit the pathogenicity of A. hydrophila. Fish treated with turmeric oil showed a reduced mortality rate of 60%, whereas all fish in the positive control group died. Moreover, treatment with turmeric oil could alleviate the renal injury. Collectively, the results suggested that targeting bacterial virulence might be a useful approach to combating bacterial infections, and turmeric oil could serve as a potential agent for combating A. hydrophila infections. Full article

Accurate monitoring of ground deformation is crucial for ensuring the safety and stability of hydraulic structures. Current deformation monitoring techniques often face challenges such as limited accuracy and robustness, particularly in complex environments. In this study, we propose a comprehensive method for Global Navigation Satellite System (GNSS) deformation monitoring in hydraulic structures by integrating the strengths of Gated Recurrent Units (GRUs) and Autoregressive Attention mechanisms. This approach enables efficient modeling of long-term dependencies while focusing on critical time steps, thereby enhancing prediction accuracy and robustness, especially in multi-step forecasting tasks. Experimental results show that the proposed GRU–Attention model achieves millimeter-level multi-step prediction accuracy, with predictions closely matching actual deformation data. Compared to the traditional method, the GRU–Attention model improves prediction accuracy by approximately 37%. The model’s attention mechanism effectively captures both short-term variations and long-term trends, ensuring accurate predictions even in complex scenarios. This research advances the field of GNSS deformation monitoring for hydraulic structures, providing valuable insights for engineering decision-making and risk management, ultimately contributing to enhanced infrastructure safety. Full article

Aeromonas hydrophila (A. hydrophila) is an opportunistic and foodborne pathogen widely spread in the environments, particularly aquatic environments. Diseases caused by A. hydrophila in freshwater aquaculture result in huge economic losses every year. The increasing emergence of antibiotic resistance has limited the application of antibiotics in aquaculture. Aerolysin (AerA), the main virulence factor produced by A. hydrophila, has been identified as a promising target for developing drugs controlling A. hydrophila infection. Here, we found that the natural compound amentoflavone (AMF) with the MIC of 512 μg/mL against A. hydrophila could dose-dependently reduce the hemolysis of AerA, ranging from 0.5 to 4 μg/mL. Molecular docking and dynamics simulation results predicted that AMF could directly bind to domain 3 of AerA via Pro333 and Trp375 residues. Then, the binding sites were confirmed by fluorescence quenching assays. The results of heptamer formation demonstrated that the binding of AMF could affect the formation of oligomers and result in the loss of pore-forming activity. Cell viability assay showed that AerA after treatment with AMF ranging from 0.5 to 4 μg/mL could significantly reduce AerA-mediated cytotoxicity. Moreover, experimental therapeutics results showed that channel catfish infected with A. hydrophila and then administered with 20 mg/kg AMF at intervals of 12 h for 3 days could increase the survival rate by 35% compared with the positive control after a 10-day observation. These findings provided a novel approach to developing anti-infective drugs and a promising candidate for controlling A. hydrophila infection in aquaculture. Full article

Background/Objectives: Fabry disease is an X-linked disorder caused by lysosomal accumulation of glycosphingolipids due to the deficiency of α-Galactosidase (α-GAL), which leads to pathology in multiple organ systems. The standard of care is enzyme replacement therapy (ERT) with recombinant native α-GAL protein. Shortcomings of the native α-GAL include low stability, a short circulating half-life, and inadequate uptake by affected tissues that limits the efficacy of ERT and could potentially reduce AAV gene therapy (GT) benefits. Cross-correction by secreted α-GAL is essential for liver-targeted as well as ubiquitous AAV GT due to poor transduction and/or short half-life of some of the significantly affected cell types. Methods: To overcome potential limitations of AAV GT delivering native α-GAL, we used an engineered GLA transgene product to improve enzyme stability and reduce predicted immunogenicity. Results: The stabilized α-GAL variant, Eng-C, had an extended circulatory half-life, allowing for enhanced distribution and efficient uptake by target organs. AAV gene therapy with Eng-C demonstrated significantly greater substrate reduction in the severe Fabry G3Stg/GlaKO mouse model across all affected tissues. Efficacy of the Eng-C AVV GT was equal to or greater than the efficacy of the higher doses of the AAV GT with native α-GAL. Furthermore, this study is the first to demonstrate that the pre-existing pathology in some tissues in G3Stg/GlaKO mice can be reversed with efficient treatment. Conclusions: Our findings demonstrate that an AAV-based gene therapy expressing an engineered α-GAL with improved stability and lower immunogenicity could be effective at lower doses than other AAV GTs, potentially offering lower safety risks typically associated with high AAV vector doses. Full article

Aeromonas veronii (A. veronii) is a ubiquitous bacterium in terrestrial and aquatic environments. It has a significant impact on animal and human health, with it becoming an emerging crucial pathogen worldwide. However, there have been no reports of mute swan infections. In the present study, after an observation of pathological changes, one bacterial strain isolated from a dead migratory mute swan was identified as A. veronii HNZZ-1/2022 based on its morphology, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and sequence analysis of the 16S rRNA and gyrB genes. To explore its pathogenicity, virulence gene detection and a gosling infection experiment were subsequently carried out, respectively. Six virulence genes for cytotonic enterotoxins (alt), lateral elastase (ela), lipase (lip), cytotoxic enterotoxin (act), aerolysin (aerA), and polar flagellin (fla) were present in the template DNA of A. veronii HNZZ-1/2022. Experimentally infected goslings exhibited hemorrhages of various different degrees in multiple organs. The half-maximal lethal dose (LD₅₀) value of A. veronii strain HNZZ-1/2022 was estimated to be 3.48 × 10⁸ colony forming units (CFUs) per mL for goslings. An antimicrobial susceptibility test showed that the A. veronii HNZZ-1/2022 strain was resistant to meropenem, ampicillin, and enrofloxacin. To date, this is the first report of A. veronii in migratory mute swans, thus expanding the currently known host spectrum. These results suggest that the migratory mute swan is a new host for A. veronii and demonstrate the need for extensive surveillance and research of A. veronii to minimize its transmission between animals, the environment, and humans. Full article

This study reports on the development and validation of a video-based instrument to assess early childhood education (ECE) teachers’ professional vision (PV) of language-stimulation (LS) interactions. PV refers to noticing and reasoning about key classroom interactions, a skill that can be trained and distinguishes experts from novices. The instrument targets the PV of three language-stimulation (LS) strategies: language input (LI), opportunities for language production (OLP), and feedback (FB). The instrument measures noticing through comparative judgement (CJ) and reasoning through multiple-choice items. Construct validity was assessed using the AERA framework, using three samples: a sample of professionals (n = 22), a pre-service teachers’ sample (n = 107), and a mixed sample with in- and pre-service teachers (n = 6). Reliability and validity were confirmed, with strong reliability scores for the CJ aggregated “master” rank orders (SRR: 0.827–0.866). Think-aloud procedures demonstrated that respondents’ decisions during CJ were mainly based on LS-relevant video features. Decisions unrelated to LS require further study. Multiple-choice reasoning items were developed from professionals’ open-ended feedback. Pre-service teacher reasoning scores showed no significant predictors. Using real classroom videos, this instrument provides an ecologically valid, scalable tool for assessing teachers’ professional vision of LS interactions. This validated instrument offers a foundation for professional development programs aimed at addressing the theory–practice gap in early language education. Full article

This research synthesis, highlighting the middle level curriculum, was created as part of a working group within the American Education Research Association (AERA) Middle level Education Research Special Interest Group (MLER SIG) to advance middle level education research. The literature review that informed this research synthesis included middle level-focused, peer-reviewed journal articles from 2016 to the present. Synthesis of the literature and iterative analysis led to organizing a middle level curriculum framework to inform middle level researchers, which included five focused areas for inquiry: (a) the curriculum and equity of experience and opportunity; (b) stakeholder power and the curriculum (development, implementation, and accountability); (c) goals and purposes for the curriculum; (d) teacher learning, roles, and enactment related to the curriculum; and (e) young adolescent well-being and experiences with the curriculum. Twenty-six research questions were developed to support new research in middle level curriculum; each question was cross-referenced with the five categories in the middle level curriculum framework to create a robust starting point for research questions. Recommendations for middle level research include (a) the need for more longitudinal research studies focusing on varying aspects of middle level education, (b) the need for more large-scale research studies examining the same context (e.g., school) or multiple contexts (e.g., school districts) over time, and (c) the need for the MLER SIG to initiate and support research studies addressing one or more aspects of this research agenda and include large-scale data collection and potentially external funding. Full article

The Successful Middle School: This We Believe calls for specialized middle-level certification/licensure and teacher development. Certification/licensure has an impact on teacher preparation and, likely, the recruitment and retention of middle-level educators. In order to continue to advocate for specialized certification/licensure, research needs to determine the nature of certification/licensure, the origins and impacts of their shifts, and the recruitment and retention of middle-level educators, especially related to their pathway. This literature review is part of the development of a research agenda for the American Educational Research Association’s (AERA) Middle Level Education Research (MLER) Special Interest Group (SIG). The purpose of this review is to examine the current status of the research related to middle-level certification/licensure and the research questions generated for the MLER SIG research agenda. The literature from the last 23 years was explored to conduct this combination of literature and scoping literature review. Gaps of research were identified, and some questions were unable to be answered through this type of study. From this literature review, it is clear that ongoing and additional research needs to be conducted related to middle-level certification/licensure related to the research questions we initially generated as part of the research agenda. Full article

The design of the space hatch door mechanisms is crucial in the aerospace field, impacting not only durability and reliability but also the life safety of astronauts during space missions. This review extensively researches vehicle doors and hatches in civil and military systems across various environments, including land, sea, deep sea, aviation, aerospace, and extreme conditions. Specially, it focuses on the structural design of hatches and related mechanisms in civil aviation and military aerospace environments, such as opening and closing mechanisms, release mechanisms, locking mechanisms, sealing mechanisms, and the ergonomic design of door structures. The review highlights the integration of bionic design principles with hatch mechanisms to explore future solutions. By systematically examining these aeras, this review addresses the lack of comprehensive studies in previous reviews, which often overlook the interconnectivity and applicability of hatch mechanisms across different fields. The absence of such holistic reviews has led to fragmented knowledge and missed opportunities for cross-industry innovation. This review aims to fill these gaps by providing a wide range of design solutions and offering insights that can enhance the development of more reliable, efficient, and safe hatch mechanisms in aerospace and other high-stakes environments. Full article

The lakes of Jianghan Plain, as an important component of the water bodies in the middle and lower reaches of the Yangtze River plain, have made significant contributions to maintaining the ecological health and promoting the sustainable development of the Jianghan Plain. However, there is a relatively limited understanding regarding the trends of lake area change for different types of lakes and their dominant factors over the past three decades in the Jianghan Plain. Based on the Google Earth Engine (GEE) platform, combined with the water body index method, the changes in area of three different types of lakes (area > 1 km²) in the Jianghan Lake Group from 1990 to 2020 were extracted and analyzed. Additionally, the Partial least squares structural equation model (PLS-SEM) was utilized to analyze the driving factors affecting the changes in water body area of these lakes. The results show that from 1990 to 2020, the area of the lakes of the wet season and level season exhibited a decreasing trend, decreasing by 893.1 km² and 77.9 km², respectively. However, the area of dry season lakes increased by 59.27 km². The areas of all three types of lakes reached their minimum values in 2006. According to the PLS-SEM results, the continuous changes in the lakes’ area are mainly controlled by environmental factors overall. Furthermore, human factors mainly influence the mutation of the lakes’ area. This study achieved precise extraction of water body areas and accurate analysis of driving factors, providing a basis for a comprehensive understanding of the dynamic changes in the lakes of Jianghan Plain, which is beneficial for the rational utilization and protection of water resources. Full article