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  • Article
  • Open Access
11 Citations
4,904 Views
14 Pages

Transfer of a Rational Crystal Contact Engineering Strategy between Diverse Alcohol Dehydrogenases

  • Brigitte Walla,
  • Daniel Bischoff,
  • Robert Janowski,
  • Nikolas von den Eichen,
  • Dierk Niessing and
  • Dirk Weuster-Botz

17 August 2021

Protein crystallization can serve as a purification step in biotechnological processes but is often limited by the non-crystallizability of proteins. Enabling or improving crystallization is mostly achieved by high-throughput screening of crystalliza...

  • Article
  • Open Access
5 Citations
3,471 Views
17 Pages

1 December 2022

The generation of cross-linked enzyme crystals is a very attractive method for immobilization of enzymes displaying high crystalizability. However, the commonly used cross-linker glutaraldehyde is not always compatible with enzyme activity. Therefore...

  • Article
  • Open Access
1 Citations
1,494 Views
18 Pages

Application of a Rational Crystal Contact Engineering Strategy on a Poly(ethylene terephthalate)-Degrading Cutinase

  • Brigitte Walla,
  • Anna-Maria Dietrich,
  • Edwin Brames,
  • Daniel Bischoff,
  • Stefanie Fritzsche,
  • Kathrin Castiglione,
  • Robert Janowski,
  • Dierk Niessing and
  • Dirk Weuster-Botz

Industrial biotechnology offers a potential ecological solution for PET recycling under relatively mild reaction conditions via enzymatic degradation, particularly using the leaf branch compost cutinase (LCC) quadruple mutant ICCG. To improve the eff...

  • Article
  • Open Access
3 Citations
1,512 Views
18 Pages

Rational Introduction of Electrostatic Interactions at Crystal Contacts to Enhance Protein Crystallization of an Ene Reductase

  • Brigitte Walla,
  • Anna Maslakova,
  • Daniel Bischoff,
  • Robert Janowski,
  • Dierk Niessing and
  • Dirk Weuster-Botz

22 March 2025

Protein crystallization is an alternative to well-established but cost-intensive and time-consuming chromatography in biotechnological processes, with protein crystallization defined as an essential unit operation for isolating proteins, e.g., active...

  • Perspective
  • Open Access
27 Citations
3,858 Views
7 Pages

11 February 2022

The structure, fundamental properties, and reactivity of chemical systems at various hierarchical levels of organization of matter is the paradigm of chemistry. A qualitative and quantitative description of various intermolecular and intramolecular n...

  • Article
  • Open Access
10 Citations
6,117 Views
18 Pages

Controlling Protein Crystallization by Free Energy Guided Design of Interactions at Crystal Contacts

  • Johannes Hermann,
  • Daniel Bischoff,
  • Phillip Grob,
  • Robert Janowski,
  • Dariusch Hekmat,
  • Dierk Niessing,
  • Martin Zacharias and
  • Dirk Weuster-Botz

24 May 2021

Protein crystallization can function as an effective method for protein purification or formulation. Such an application requires a comprehensive understanding of the intermolecular protein–protein interactions that drive and stabilize protein crysta...

  • Review
  • Open Access
603 Views
14 Pages

31 December 2025

X-ray crystallography remains the gold standard for high-resolution structural biology, yet obtaining diffraction-quality crystals continues to pose a major bottleneck due to inherently low success rates. This review advocates a paradigm shift from p...

  • Article
  • Open Access
2,787 Views
13 Pages

7 October 2023

3-chloro-4-hydroxyphenylacetic acid (CHPAA) is a fungal metabolite. It is a small molecule that is useful in crystal engineering studies due to the functional groups present. Six amines were selected to form salts with CHPAA. Linear derivatives inclu...

  • Review
  • Open Access
12 Citations
5,671 Views
16 Pages

Engineering Crystal Packing in RNA Structures I: Past and Future Strategies for Engineering RNA Packing in Crystals

  • Narsimha Pujari,
  • Stephanie L. Saundh,
  • Francis A. Acquah,
  • Blaine H. M. Mooers,
  • Adrian R. Ferré-D’Amaré and
  • Adelaine Kwun-Wai Leung

15 August 2021

X-ray crystallography remains a powerful method to gain atomistic insights into the catalytic and regulatory functions of RNA molecules. However, the technique requires the preparation of diffraction-quality crystals. This is often a resource- and ti...

  • Review
  • Open Access
23 Citations
5,687 Views
16 Pages

General Strategies for RNA X-ray Crystallography

  • Ryland W. Jackson,
  • Claire M. Smathers and
  • Aaron R. Robart

23 February 2023

An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure,...

  • Review
  • Open Access
13 Citations
2,944 Views
20 Pages

16 April 2021

The Cambridge Structural Database was surveyed for crystals featuring I⋯Br secondary-bonding in their supramolecular assemblies occurring independently of other obvious supramolecular synthons and devoid of other halogen bonding interactions. In all,...

  • Review
  • Open Access
12 Citations
4,138 Views
13 Pages

Photoaligned Liquid Crystalline Structures for Photonic Applications

  • Aleksey Kudreyko,
  • Vladimir Chigrinov,
  • Gurumurthy Hegde and
  • Denis Chausov

17 June 2023

With the advancement of information display technologies, research on liquid crystals is undergoing a tremendous shift to photonic devices. For example, devices and configurations based on liquid crystal materials are being developed for various appl...

  • Article
  • Open Access
3 Citations
3,415 Views
15 Pages

28 March 2024

Cracks and crack-like defects in engineering structures have greatly reduced the structural strength. An interface crack with one contact area in a combined tension–shear field of decagonal quasicrystal bi-material is investigated. Based on the...

  • Article
  • Open Access
15 Citations
3,048 Views
13 Pages

Crystal Structure and Noncovalent Interactions of Heterocyclic Energetic Molecules

  • Yan Liu,
  • Jiake Fan,
  • Zhongqing Xue,
  • Yajing Lu,
  • Jinan Zhao and
  • Wenyan Hui

4 August 2022

Nitrogen-rich heterocyclic compounds are important heterocyclic substances with extensive future applications for energetic materials due to their outstanding density and excellent physicochemical properties. However, the weak intermolecular interact...

  • Article
  • Open Access
13 Citations
4,772 Views
17 Pages

23 October 2021

The dovetail attachment between the turbine blade and disk for an aero-engine operates under varying centrifugal load and vibration at elevated temperatures. The fretting fatigue is prone to occur at the contact surface of the dovetail attachment. Th...

  • Article
  • Open Access
5 Citations
4,124 Views
9 Pages

25 February 2020

Recently, gallium telluride (GaTe) has triggered much attention for its unique properties and offers excellent opportunities for nanoelectronics. Yet it is a challenge to bridge the semiconducting few-layered GaTe crystals with metallic electrodes fo...

  • Article
  • Open Access
6 Citations
6,900 Views
24 Pages

Crystal Structure and Characterization of Human Heavy-Chain Only Antibodies Reveals a Novel, Stable Dimeric Structure Similar to Monoclonal Antibodies

  • Carl Mieczkowski,
  • Soheila Bahmanjah,
  • Yao Yu,
  • Jeanne Baker,
  • Gopalan Raghunathan,
  • Daniela Tomazela,
  • Mark Hsieh,
  • Mark McCoy,
  • Corey Strickland and
  • Laurence Fayadat-Dilman

22 November 2020

We report the novel crystal structure and characterization of symmetrical, homodimeric humanized heavy-chain-only antibodies or dimers (HC2s). HC2s were found to be significantly coexpressed and secreted along with mAbs from transient CHO HC/LC cotra...

  • Article
  • Open Access
1 Citations
914 Views
14 Pages

26 May 2025

Aerospace engines ingest small particles when operating in a particulate-rich environment, such as sandstorms, atmospheric pollution, and volcanic ash clouds. These micron particles enter their cooling channels, leading to film-cooling hole blockage...

  • Article
  • Open Access
616 Views
15 Pages

Femtosecond Laser-Processed, Copper-Coated Stainless Steel Implants Promoting In Situ Calcium Phosphate Crystallization for Orthopedic Application

  • Albena Daskalova,
  • Maja Dutour Sikirić,
  • Liliya Angelova,
  • Tihomir Car,
  • Ana-Marija Milisav,
  • Stuart Neil and
  • Abeer Shaalan

5 November 2025

Today, the engineering of load-bearing bone tissue after severe trauma still relies on metal-based (Ti, CoCrMo alloys or stainless steel) permanent implants. Such artificial scaffolds are typically applied in the body and come into direct contact wit...

  • Opinion
  • Open Access
5 Citations
2,473 Views
2 Pages

6 February 2021

Both Cambridge Crystallographic Data Centre (CCDC) and Protein Data Bank (PDB) provide unique opportunities for finding information about the structures of chemical and biochemical compounds in the solid state. The IsoStar—a knowledge-based library o...

  • Article
  • Open Access
15 Citations
6,327 Views
10 Pages

19 March 2021

To enable highly conductive electronic textiles (E-textiles), we herein demonstrate a simple solution treatment of poly (3,4-ethylenedioxythiophene): poly (styrene sulfonate) (PEDOT:PSS)-coated textiles by dimethyl sulfoxide (DMSO) and methanol. The...

  • Article
  • Open Access
6 Citations
4,103 Views
9 Pages

Efficient CsPbBr3 Perovskite Solar Cells with Storage Stability > 340 Days

  • Shaochuan Hou,
  • Siheng Wu,
  • Xiaoyan Li,
  • Jiahao Yan,
  • Jie Xing,
  • Hao Liu,
  • Huiying Hao,
  • Jingjing Dong and
  • Haochong Huang

19 October 2022

For CsPbBr3 perovskite materials, it is especially important to reduce interface defects, suppress non-radiative recombination, and improve morphology to achieve highly efficient and stable CsPbBr3 perovskite solar cells (PSCs). Herein, we reported a...

  • Feature Paper
  • Review
  • Open Access
21 Citations
6,248 Views
40 Pages

28 November 2022

Temperature is one of the most important parameters in the combustion processes. Accurate surface temperature can help to gain insight into the combustion characteristics of various solid or liquid fuels, as well as to evaluate the operating status o...

  • Article
  • Open Access
5 Citations
2,340 Views
23 Pages

24 May 2024

A series of novel salts of heterocyclic polyamines with 5-sulfosalicylic acid (C4H7N4+)(C7H5O6S)∙2(H2O) (1), (C4H6ClN4+)(C7H5O6S)∙H2O (2), (C5H8N3+)(C7H5O6S)∙H2O (3), (C5H7N6+)(C7H5O6S)∙H2O (4), (C6...

  • Article
  • Open Access
1,105 Views
13 Pages

27 November 2024

In this study, unconventional C—Hlg···H–C (Hlg = Cl, Br, and I) interactions involving sp, sp2, and sp3 organic halides were investigated at the RI-MP2/aug-cc-pVQZ level of theory. Energy Decomposition Analyses (EDA)...

  • Review
  • Open Access
1 Citations
12,680 Views
22 Pages

18 September 2025

Bicalutamide (BCL), a clinically important non-steroidal antiandrogen, exhibits pronounced conformational polymorphism and complex solid-state behaviour that critically influence its physicochemical and biopharmaceutical properties. This review compr...

  • Feature Paper
  • Article
  • Open Access
2 Citations
3,813 Views
16 Pages

Material Removal Model for Lapping Process Based on Spiral Groove Density

  • Taekyung Lee,
  • Haedo Jeong,
  • Sangjik Lee,
  • Hanchul Cho,
  • Doyeon Kim and
  • Hyoungjae Kim

27 April 2021

The increasing demand for single-crystal wafers combined with the increase in diameter of semiconductor wafers has warranted further improvements in thickness variation and material removal rate during lapping to ensure price competitiveness of wafer...

  • Article
  • Open Access
789 Views
22 Pages

A Study on Improving the Macro- and Micromechanical Properties of Loess Deposits from the Yili Basin: Enzyme-Induced Carbonate Precipitation (EICP) Technique

  • Yuan Xue,
  • Ai Zhang,
  • Guangming Shi,
  • Dejun Yang,
  • Weiming Guan,
  • Junhui Zhang,
  • Xinhui Pan,
  • Dingyu Wang and
  • Chao Jin

16 November 2025

China’s loess deposits exhibit high vulnerability to deformation under precipitation and snowmelt, posing significant risks to infrastructure. This study utilized enzyme-induced carbonate precipitation (EICP) to enhance the mechanical propertie...

  • Article
  • Open Access
15 Citations
4,488 Views
10 Pages

Direct Experimental Evidence of Biomimetic Surfaces with Chemical Modifications Interfering with Adhesive Protein Adsorption

  • Hui Yang,
  • Wei Zhang,
  • Ting Chen,
  • Shizhe Huang,
  • Baogang Quan,
  • Min Wang,
  • Junjie Li,
  • Changzhi Gu and
  • Jinben Wang

21 December 2018

Current approaches to dealing with the worldwide problem of marine biofouling are to impart chemical functionality to the surface or utilize microtopography inspired by nature. Previous reports have shown that only introducing a single method may not...

  • Article
  • Open Access
17 Citations
4,182 Views
19 Pages

A Role of Coagulant in Structure Formation of Fibers and Films Spun from Cellulose Solutions

  • Valery Kulichikhin,
  • Igor Makarov,
  • Maria Mironova,
  • Lyudmila Golova,
  • Markel Vinogradov,
  • Georgiy Shandryuk,
  • Ivan Levin and
  • Natalia Arkharova

7 August 2020

Replacing the aqueous coagulation bath with an alcoholic one during spinning cellulose fibers (films) from solutions in N-methylmorpholine-N-oxide leads to a radical restructuring of the hydrogen bonds net of cellulose and, as a result, to a change i...

  • Article
  • Open Access
2 Citations
3,296 Views
13 Pages

3 May 2023

In order to address the issue of vibration, it is crucial to accurately measure the vibration distribution. The authors previously developed the one-pitch phase analysis (OPPA) method, which allows for rapidly capturing the three-dimensional shape of...

  • Article
  • Open Access
11 Citations
4,158 Views
17 Pages

Preparation and Properties of Stereocomplex of Poly(lactic acid) and Its Amphiphilic Copolymers Containing Glucose Groups

  • Liyan Qi,
  • Qianjin Zhu,
  • Dan Cao,
  • Tingting Liu,
  • Kevin R Zhu,
  • Kaixin Chang and
  • Qinwei Gao

31 March 2020

The stereocomplex of poly(lactic acid) containing glucose groups (sc-PLAG) was prepared by solution blending from equal amounts of poly(l-lactic acid) (PLLA) and poly(d-lactic acid-co-glucose) (PDLAG), which were synthesized from l- and d-lactic acid...

  • Article
  • Open Access
12 Citations
3,289 Views
24 Pages

3 November 2022

The development of scaffolds that simultaneously provide porous architectures and osteogenic properties is the major challenge in tissue engineering. Herein, a scaffold with high porosity and well interconnected networks, namely poly(lactic acid)/pol...

  • Feature Paper
  • Article
  • Open Access
6 Citations
4,676 Views
18 Pages

The Wnt signalling pathway plays an important role in cell proliferation, differentiation, and fate decisions in embryonic development and the maintenance of adult tissues. The twelve armadillo (ARM) repeat-containing protein β-catenin acts as the si...

  • Article
  • Open Access
6 Citations
2,876 Views
16 Pages

24 April 2021

Freeze-thaw cycles caused by seasonal temperature fluctuations significantly affect the geotechnical engineering properties. This study investigated the crucial role of water distribution patterns in the characterization of elastic wave properties fo...

  • Article
  • Open Access
20 Citations
2,409 Views
20 Pages

4 November 2022

Condensation frosting usually causes a negative influence on heat exchangers employed in engineering fields. As the relationships among the first three typical condensation frosting stages in the edge regions of cold plates are still unclear, an expe...

  • Article
  • Open Access
20 Citations
4,764 Views
13 Pages

Star-Shaped Fe3-xO4-Au Core-Shell Nanoparticles: From Synthesis to SERS Application

  • Thi Thuy Nguyen,
  • Stephanie Lau-Truong,
  • Fayna Mammeri and
  • Souad Ammar

10 February 2020

In this work, the preparation of magneto-plasmonic granular nanostructures and their evaluation as efficient substrates for magnetically assisted surface enhanced Raman spectroscopy (SERS) sensing are discussed. These nanostructures consist of star-s...

  • Review
  • Open Access
43 Citations
8,823 Views
41 Pages

27 November 2020

Semiconducting metal oxide-based nanowires (SMO-NWs) for gas sensors have been extensively studied for their extraordinary surface-to-volume ratio, high chemical and thermal stabilities, high sensitivity, and unique electronic, photonic and mechanica...

  • Article
  • Open Access
13 Citations
3,189 Views
18 Pages

7 January 2021

In this study, tap water is magnetized by a self-developed device. The conductivity and evaporation of magnetized water (MW) at different temperatures are tested to demonstrate the magnetization mechanism. The results show that the conductivity and e...

  • Article
  • Open Access
6 Citations
2,513 Views
17 Pages

X-ray Crystallographic Study of Preferred Spacing by the NF-κB p50 Homodimer on κB DNA

  • Norman Zhu,
  • Matthew Mealka,
  • Shane Mitchel,
  • Christy Milani,
  • Lisa M. Acuña,
  • Eric Rogers,
  • Ashlee N. Lahana and
  • Tom Huxford

26 August 2023

Though originally characterized as an inactive or transcriptionally repressive factor, the NF-κB p50 homodimer has become appreciated as a physiologically relevant driver of specific target gene expression. By virtue of its low affinity for cyt...

  • Article
  • Open Access
332 Views
13 Pages

8 February 2026

Background: Orthodontic appliances introduce new surfaces into the oral cavity that can modulate biofilm formation and potentially increase the risk of white spot lesions. Material-dependent differences in surface roughness, wettability and geometry...

  • Article
  • Open Access
31 Citations
5,852 Views
19 Pages

28 May 2017

Thermally induced damage has an important influence on rock mechanics and engineering, especially for high-level radioactive waste repositories, geological carbon storage, underground coal gasification, and hydrothermal systems. Additionally, the wid...

  • Article
  • Open Access
12 Citations
6,083 Views
15 Pages

Development of β Type Ti23Mo-45S5 Bioglass Nanocomposites for Dental Applications

  • Karolina Jurczyk,
  • Andrzej Miklaszewski,
  • Mieczyslawa U. Jurczyk and
  • Mieczyslaw Jurczyk

25 November 2015

Titanium β-type alloys attract attention as biomaterials for dental applications. The aim of this work was the synthesis of nanostructured β type Ti23Mo-x wt % 45S5 Bioglass (x = 0, 3 and 10) composites by mechanical alloying and powder metallurgy me...

  • Review
  • Open Access
8 Citations
6,208 Views
21 Pages

GaAs Nanowires Grown by Catalyst Epitaxy for High Performance Photovoltaics

  • Ying Wang,
  • Xinyuan Zhou,
  • Zaixing Yang,
  • Fengyun Wang,
  • Ning Han,
  • Yunfa Chen and
  • Johnny C. Ho

29 August 2018

Photovoltaics (PVs) based on nanostructured III/V semiconductors can potentially reduce the material usage and increase the light-to-electricity conversion efficiency, which are anticipated to make a significant impact on the next-generation solar ce...

  • Article
  • Open Access
26 Citations
5,483 Views
13 Pages

Layered Double Hydroxide Clusters as Precursors of Novel Multifunctional Layers: A Bottom-Up Approach

  • Cristina S. Neves,
  • Alexandre C. Bastos,
  • Andrei N. Salak,
  • Maksim Starykevich,
  • Daisy Rocha,
  • Mikhail L. Zheludkevich,
  • Angela Cunha,
  • Adelaide Almeida,
  • João Tedim and
  • Mário G.S. Ferreira

The specific microstructure of aluminum alloys is herein explored to grow spatially-resolved layered double hydroxide (SR-LDH) clusters on their surface. Upon chemical modification of LDHs via intercalation, adsorption and grafting with different fun...

  • Article
  • Open Access
1 Citations
2,167 Views
14 Pages

13 December 2022

To meet aero-engine aluminum skirt requirements, an experiment was carried out using Al–Nb–B2O3–CuO as the reaction system and a 6063 aluminum alloy melt as the reaction medium for a contact reaction, and 6063 aluminum matrix compos...

  • Article
  • Open Access
301 Views
21 Pages

Analysis of Wind Erosion Resistance Enhancement of Aeolian Sand by Microbially Induced Carbonate Precipitation Technology

  • Fangcan Ji,
  • Junhui Zhang,
  • Weiming Guan,
  • Hui Chen,
  • Xin Wang,
  • Meng Xie,
  • Haosen Wang and
  • Defeng Hou

7 January 2026

Aeolian sand in arid mining regions is highly susceptible to wind erosion, posing serious threats to ecological stability and surface engineering safety. To enhance its resistance, this study applied the microbially induced carbonate precipitation (M...

  • Article
  • Open Access
9 Citations
2,509 Views
13 Pages

16 July 2020

The current research reports the improvement in surface integrity and tribological characteristics of steel prepared using a powder metallurgy (PM) by ultrasonic nanocrystal surface modification (UNSM) at 25 and 300 °C. The surface integrity and...

  • Article
  • Open Access
1,372 Views
23 Pages

Synergistic Regulation of δ-MnO2 Cathode via Crystal Engineering and pH Buffering for Long-Cycle Aqueous Zinc-Ion Batteries

  • Fan Zhang,
  • Haotian Yu,
  • Qiongyue Zhang,
  • Yahao Wang,
  • Haodong Ren,
  • Huirong Liang,
  • Jinrui Li,
  • Yuanyuan Feng,
  • Bin Zhao and
  • Xiaogang Han

8 October 2025

Aqueous zinc-ion batteries (ZIBs) have emerged as a promising candidate for large-scale energy storage due to their inherent safety, low cost, and environmental friendliness. However, manganese dioxide (MnO2)-based cathodes, which are widely studied...

  • Article
  • Open Access
568 Views
22 Pages

Zn–IMP 3D Coordination Polymers for Drug Delivery: Crystal Structure and Computational Studies

  • Hafiz Zeshan Aqil,
  • Yanhong Zhu,
  • Masooma Hyder Khan,
  • Yaqoot Khan,
  • Beenish Sandhu,
  • Muhammad Irfan and
  • Hui Li

31 December 2025

Coordination polymers (CPs) are garnering attention in the field of medicine day by day. The goal is to develop a CP with biosafe and environment-friendly characteristics. Herein, we report two such novel 3D coordination polymers of zinc-inosine-5&pr...

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