A starch-based ethanol facility producing 200,000 m
3 ethanol/year also produces
ca. 2 million m
3 thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a
Zygomycete and four
Ascomycetes were successfully grown
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A starch-based ethanol facility producing 200,000 m
3 ethanol/year also produces
ca. 2 million m
3 thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a
Zygomycete and four
Ascomycetes were successfully grown in thin stillage containing 9% solids. Cultivation with
Neurospora intermedia led to the production of
ca. 16 g·L
−1 biomass containing 56% (w/w) crude protein, a reduction of 34% of the total solids, and 5 g·L
−1 additional ethanol. In an industrial ethanol production process (200,000 m
3 ethanol/year), this can potentially lead to the production of 11,000 m
3 extra ethanol per year. Cultivation with
Aspergillus oryzae resulted in 19 g·L
−1 biomass containing 48% (w/w) crude protein and the highest reduction of the thin stillage glycerol (54%) among the
Ascomycetes. Cultivation with
Rhizopus sp
. produced up to 15 g·L
−1 biomass containing 55% (w/w) crude protein. The spent thin stillage had been reduced up to 85%, 68% and 21% regarding lactic acid, glycerol and total solids, respectively. Therefore,
N. intermedia, in particular, has a high potential to improve the ethanol process via production of additional ethanol and high-quality biomass, which can be considered for animal feed applications such as for fish feed.
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