Impact of Transgenic Maize Ruifeng125 on Diversity and Dynamics of Bacterial Community in Rhizosphere Soil
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Test Design
2.3. Collection of Rhizosphere Soil
2.4. DNA Extraction and PCR Amplification
2.5. Detection of Bt Protein Contents in the Rhizosphere Soil
2.6. Illumina Miseq Sequencing and Data Processing
3. Results
3.1. The Accumulation and Degradation Dynamics of Bt Protein in the Rhizosphere Soil
3.2. Statistics and Analysis of Sequencing Data
3.3. Changes of Bacterial Community Structure in Rhizosphere Soils of Transgenic Maize Ruifeng125 and Its Control Line
3.4. Beta Diversity of Rhizosphere Bacterial Community of the Two Maize Lines
3.5. Difference in Bacterial Community Abundance in Two Rhizosphere Soils
3.6. Biomarkers between Transgenic Maize Ruifeng125 and Non-Transgenic Control along the Six Developmental Stages
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Degragation (d) | Bt Protein Content (ng/g) | Protein Degradation Rate |
---|---|---|
0 | 937.60 | 0 |
1 | 781.68 | 16.63% |
2 | 549.53 | 41.39% |
3 | 382.92 | 59.16% |
4 | 247.71 | 73.58% |
5 | 152.74 | 83.71% |
6 | 89.82 | 90.42% |
7 | 62.26 | 93.36% |
10 | 25.69 | 97.26% |
20 | 16.50 | 98.24% |
40 | 7.69 | 99.18% |
70 | 1.97 | 99.79% |
110 | 0.66 | 99.93% |
160 | 0.35 | 99.97% |
170 | -- | 100% |
Group | Valid Reads | Average Length | Shannon Index | Chao Index | ACE Index | Simpson Index | Coverage |
---|---|---|---|---|---|---|---|
Z | 38,634 ± 4115 | 439 ± 0.75 | 6.99 ± 0.10 | 4157 ± 289 | 4160 ± 281 | 0.0024 ± 0.0003 | 0.96 ± 0.008 |
D | 38,168 ± 4643 | 438.934 ± 0.79 | 7.00 ± 0.14 | 4051 ± 268 | 4070 ± 254 | 0.0025 ± 0.0012 | 0.96 ± 0.008 |
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Hao, C.; Xia, X.; Xu, C.; Sun, H.; Li, F.; Yang, S.; Xu, X.; Lu, X. Impact of Transgenic Maize Ruifeng125 on Diversity and Dynamics of Bacterial Community in Rhizosphere Soil. Microorganisms 2024, 12, 1763. https://doi.org/10.3390/microorganisms12091763
Hao C, Xia X, Xu C, Sun H, Li F, Yang S, Xu X, Lu X. Impact of Transgenic Maize Ruifeng125 on Diversity and Dynamics of Bacterial Community in Rhizosphere Soil. Microorganisms. 2024; 12(9):1763. https://doi.org/10.3390/microorganisms12091763
Chicago/Turabian StyleHao, Chaofeng, Xinyao Xia, Chao Xu, Hongwei Sun, Fan Li, Shuke Yang, Xiaohui Xu, and Xingbo Lu. 2024. "Impact of Transgenic Maize Ruifeng125 on Diversity and Dynamics of Bacterial Community in Rhizosphere Soil" Microorganisms 12, no. 9: 1763. https://doi.org/10.3390/microorganisms12091763