Animals

17 pages, 1924 KiB

Open AccessArticle

Quill Mites of the Family Syringophilidae (Acariformes: Cheyletoidea) Parasitising Birds of the Subfamily Euphoninae (Passeriformes: Fringillidae)

by Bozena Sikora, Markus Unsoeld, Roland R. Melzer, Stefan Friedrich, Martin Hromada and Maciej Skoracki

Animals 2025, 15(5), 764; https://doi.org/10.3390/ani15050764 - 6 Mar 2025

Viewed by 501

Abstract

Quill mites of the family Syringophilidae (Acariformes: Prostigmata) are highly specialised avian ectoparasites that inhabit feather quills. Despite their widespread occurrence, their diversity, distribution, and host associations remain poorly understood. This study examined the diversity and ecological interactions of syringophilid mites parasitising Euphoninae [...] Read more.

Quill mites of the family Syringophilidae (Acariformes: Prostigmata) are highly specialised avian ectoparasites that inhabit feather quills. Despite their widespread occurrence, their diversity, distribution, and host associations remain poorly understood. This study examined the diversity and ecological interactions of syringophilid mites parasitising Euphoninae hosts. We analysed 298 dry bird skins representing 25 species deposited in the Bavarian State Collection for Zoology in Munich, Germany. Quill mite infestations were detected in 15 host species, identifying 4 mite species, including 2 newly described taxa: Aulonastus neotropicalis sp. n. and Syringophilopsis euphonicus sp. n. Infestation prevalence ranged from 2% to 25%. Quill mite–host interactions exhibited high specialisation and niche differentiation, with no co-occurring species sharing the same microhabitat. Network analysis indicated moderate connectance (0.35) and significant host specificity (H2′ = 0.77, p = 0.007). Biogeographic history suggests that divergence from Carduelinae and subsequent evolutionary events shaped syringophilid diversity in Euphoninae. These findings underscore the importance of museum collections in uncovering cryptic parasite diversity and provide new insights into host–parasite co-evolutionary dynamics. Full article

(This article belongs to the Section Wildlife)

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13 pages, 701 KiB

Open AccessArticle

Effects of Precision Feeding on Economic and Productive Yields of Hyperprolific Lactating Sows Allocated at Different Farrowing Pens

by María Aparicio-Arnay, Natalia Yeste-Vizcaíno, Nerea Soria, Jorge Cambra, Beatriz Isabel, Carlos Piñeiro and Antonio Gonzalez-Bulnes

Animals 2025, 15(5), 763; https://doi.org/10.3390/ani15050763 - 6 Mar 2025

Cited by 1 | Viewed by 661

Abstract

The results obtained using precision feeding in maternities (electronic sow feeders, ESFs) may be affected by the type of farrowing pen, as the design of the pen may modify the behavior of the sows. Hence, the aim of the present study was to [...] Read more.

The results obtained using precision feeding in maternities (electronic sow feeders, ESFs) may be affected by the type of farrowing pen, as the design of the pen may modify the behavior of the sows. Hence, the aim of the present study was to determine the effects and possible interactions of electronic feeding systems in different types of farrowing pens (traditional, socialization and loose housing pens) on the productive and economic yields of lactating hyperprolific sows under commercial farm conditions. The results showed an overall lower feed disappearance in sows fed with ESFs and, consequently, a lower amount of feed per weaned piglet and per kg of weaned piglet, with evidence of improved welfare and no negative effects on body weight, condition at weaning or reproductive yields in the following cycle. These effects were, however, modulated by the type of pen and the type of bawl feeder; this finding highlights the need for improvement in the design of facilities and especially feeders to allow the sow a better use of available feed. Full article

(This article belongs to the Section Animal System and Management)

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11 pages, 1175 KiB

Open AccessArticle

A New Research Tool for Use in Sharks and Rays: Relevance of Reproductive Hormone Levels in the Skin of Small-Spotted Catshark (Scyliorhinus canicula)

by Annaïs Carbajal, Isabel González Lobato, Clara Agustí, Marta Muñoz-Baquero, Paula Serres-Corral and Manel López-Béjar

Animals 2025, 15(5), 762; https://doi.org/10.3390/ani15050762 - 6 Mar 2025

Viewed by 646

Abstract

The present study marks a significant step forward in validating the use of skin biopsies for measuring reproductive steroid hormones in sharks. Given the conservation concerns surrounding many chondrichthyan species and the growing emphasis on adhering to the 3R principles in wildlife research, [...] Read more.

The present study marks a significant step forward in validating the use of skin biopsies for measuring reproductive steroid hormones in sharks. Given the conservation concerns surrounding many chondrichthyan species and the growing emphasis on adhering to the 3R principles in wildlife research, we should prioritize the acquisition of data through non-destructive or minimally invasive sampling techniques collected remotely. Dart skin biopsy samples can be collected remotely, without the need to chase, capture, and restrain the animal. Accordingly, the present study aimed to (i) develop a suitable extraction and analytical technique for reproductive hormone determination in shark skin biopsies and (ii) evaluate whether sex steroid hormones detected in shark skin differ between males and females and depending on males’ maturity. The results of assay validation demonstrated that the commercial enzyme immunoassay used can provide reliable measures of progesterone, 17β-estradiol, and testosterone measured in shark skin extracts. After ensuring that reproductive hormones are accurately measured in this sample type, we compared hormone levels between males (mature and immature) and females to evaluate the variation in reproductive hormones relative to sex and males’ maturity stage. We detected significant differences in skin hormone levels between sexes, suggesting that this technique may be a promising alternative approach for obtaining relevant biological data in free-ranging sharks. By employing this novel technique, we expect to gain a deeper understanding of the reproductive processes of living chondrichthyans, which is essential for formulating effective science-based conservation and management strategies. Full article

(This article belongs to the Section Animal Welfare)

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22 pages, 2419 KiB

Open AccessArticle

Multivariable Analysis of the Association Between Lumbar and Lumbosacral MRI-Diagnosed Spinal Pathologies and Pain in Dogs

by Roger Medina-Serra, Patricia López-Abradelo, Eliseo Belda, Holly Riding-Medina, Francisco G. Laredo, Rachel Marwood, Verónica Mortera and José I. Redondo

Animals 2025, 15(5), 761; https://doi.org/10.3390/ani15050761 - 6 Mar 2025

Viewed by 1026

Abstract

Lumbar and lumbosacral pain in dogs often involves multiple concurrent spinal pathologies, complicating the identification of primary pain generators. This study assessed the associations between MRI-diagnosed spinal pathologies and pain to provide clinically relevant insights for their diagnosis and management. MRI scans and [...] Read more.

Lumbar and lumbosacral pain in dogs often involves multiple concurrent spinal pathologies, complicating the identification of primary pain generators. This study assessed the associations between MRI-diagnosed spinal pathologies and pain to provide clinically relevant insights for their diagnosis and management. MRI scans and clinical records of 518 client-owned dogs were retrospectively reviewed, documenting demographic data, pain status, and MRI findings. Multivariable logistic regression models evaluated the associations between spinal pathologies and pain, adjusting for age and weight. The intervertebral disc (IVD) extrusion was the primary pathology associated with lumbar pain, while radiculopathy had the strongest association with lumbosacral pain. Additional lumbosacral pathologies, including foraminal stenosis, IVD bulging, and IVD protrusion, were also significantly associated with pain. However, some dogs with MRI-diagnosed abnormalities showed no pain, whereas others with pain had no detectable MRI pathology, underscoring the need to interpret imaging within the clinical context. Pain responses observed during physical examination often overlapped between spinal and hip conditions, complicating diagnostic accuracy. These findings emphasise the importance of integrating imaging, clinical assessment, and targeted diagnostic techniques to improve pain localisation and treatment decisions, providing veterinarians with valuable data to refine the management of lumbar and lumbosacral pain in dogs. Full article

(This article belongs to the Special Issue Diagnostic Approach and Treatment of Orthopedic Disease in Dogs and Cats: Second Edition)

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18 pages, 4352 KiB

Open AccessArticle

Ecotones in the Spotlight—Habitat Selection of the Golden Jackal (Canis aureus Linnaeus, 1758) in the Agricultural Landscapes of Central Europe

by Dorottya Karolin Gaál, Miklós Heltai, Gyula Sándor, Gergely Schally and Erika Csányi

Animals 2025, 15(5), 760; https://doi.org/10.3390/ani15050760 - 6 Mar 2025

Viewed by 613

Abstract

The large-scale expansion of the golden jackal (Canis aureus) across Europe in recent decades has been strongly influenced by its successful space and habitat use. In this study, we analyzed the habitat selection of seven golden jackals tracked with GPS collars [...] Read more.

The large-scale expansion of the golden jackal (Canis aureus) across Europe in recent decades has been strongly influenced by its successful space and habitat use. In this study, we analyzed the habitat selection of seven golden jackals tracked with GPS collars between 15 March 2021 and 25 November 2022 in a predominantly agricultural landscape in the southwestern part of the Pannonian Basin, Central Europe. Animals were tracked for an average of 29 weeks, and GPS collars recorded a total of 29,840 hourly localization points, which were compared to a high-resolution land cover dataset. We found that golden jackals maintain smaller home ranges in agricultural landscapes than in more pristine environments. Based on Jacobs’ index values calculated for monthly habitat preferences and the distribution of distances from land cover edges, we also found that preferences for the various habitat types differed significantly among individuals. Most of the time, golden jackals stayed near the edges of forests, agricultural lands, and shrublands, while they stayed away from artificial areas, wetlands, and water bodies. Forests and shrublands providing cover and safety were generally preferred by the golden jackals, especially during breeding and pup-rearing periods, while there was a strong avoidance of agricultural lands in general. Overall, our findings suggest that despite individual differences in the availability of habitat types within home ranges, forest–agricultural ecotones with relative proximity to food and shelter play a key role in the habitat selection of golden jackals. Full article

(This article belongs to the Section Wildlife)

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13 pages, 616 KiB

Open AccessArticle

Effect of Dietary Cannabis sativa L. Residue Supplementation on Meat Quality and Flavor-Enhancing Free Amino Acids in Broiler Chickens

by Yusup Sopian, Katatikarn Sahatsanon, Apinya Satsook, Chaiwat Arjin, Korawan Sringarm, Chompunut Lumsangkul, Panneepa Sivapirunthep and Chanporn Chaosap

Animals 2025, 15(5), 759; https://doi.org/10.3390/ani15050759 - 6 Mar 2025

Viewed by 615

Abstract

This study investigated the effects of dietary Cannabis sativa residues (CR) on broiler growth and meat characteristics. A total of 256 one-day-old male Ross-308 broilers were randomly assigned to four treatments: a basal diet (control) and diets containing 0.5%, 1%, and 2% CR. [...] Read more.

This study investigated the effects of dietary Cannabis sativa residues (CR) on broiler growth and meat characteristics. A total of 256 one-day-old male Ross-308 broilers were randomly assigned to four treatments: a basal diet (control) and diets containing 0.5%, 1%, and 2% CR. CR supplementation had no effect on growth performance, survival rate, or European production index but reduced average daily feed intake (p < 0.01). No significant differences were found in meat pH, color, drip loss, thawing loss, cooking loss, or shear force (p > 0.05). However, the ΔE values (0.5% CR: 3.97, 1.0% CR: 3.71, 2.0% CR: 4.95) indicated perceptible color differences compared to the control. CR significantly reduced C12:0, C20:1n9, and C22:1n9 fatty acids (p < 0.05) while increasing free amino acids, including aspartic acid, serine, proline, methionine, and phenylalanine (p < 0.05). It also increased moisture content and decreased fat content, especially at 2% CR (p < 0.05). In conclusion, CR supplementation improves the flavor and nutritional value of broiler meat and is a potential alternative to conventional feed additives. Full article

(This article belongs to the Section Animal Nutrition)

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28 pages, 5549 KiB

Open AccessReview

Gut Microbiota of Ruminants and Monogastric Livestock: An Overview

by Giuseppe Tardiolo, Deborah La Fauci, Valentina Riggio, Matteo Daghio, Eleonora Di Salvo, Alessandro Zumbo and Anna Maria Sutera

Animals 2025, 15(5), 758; https://doi.org/10.3390/ani15050758 - 6 Mar 2025

Viewed by 1157

Abstract

The diversity and composition of the gut microbiota are widely recognized as fundamental factors influencing the well-being and productivity of domestic animals. Advancements in sequencing technologies have revolutionized studies in this research field, allowing for deeper insights into the composition and functionality of [...] Read more.

The diversity and composition of the gut microbiota are widely recognized as fundamental factors influencing the well-being and productivity of domestic animals. Advancements in sequencing technologies have revolutionized studies in this research field, allowing for deeper insights into the composition and functionality of microbiota in livestock. Ruminants and monogastric animals exhibit distinct digestive systems and microbiota characteristics: ruminants rely on fermentation, while monogastrics use enzymatic digestion, and monogastric animals have simpler stomach structures, except for horses and rabbits, where both processes coexist. Understanding the gut microbiota’s impact and composition in both animal types is essential for optimizing production efficiency and promoting animal health. Following this perspective, the present manuscript review aims to provide a comprehensive overview of the gut microbiota in ruminants (such as cattle, sheep, and goats) and monogastric animals (including horses, pigs, rabbits, and chickens). Full article

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16 pages, 8472 KiB

Open AccessArticle

Personality Traits of the Territorial Crustacean Chinese Mitten Crab (Eriocheir sinensis): Behavioral Adaptations to Environmental Cues

by Peiqiong Fang, Sisi Sheng, Yiming Li, Yuan Li, Renhua Mo, Han Mei, Guangzhen Jiang, Wenbin Liu and Hengtong Liu

Animals 2025, 15(5), 757; https://doi.org/10.3390/ani15050757 - 6 Mar 2025

Viewed by 615

Abstract

Territorial crustaceans exhibit a complex set of personality traits and behavioral characteristics that are critical for their survival, growth, and overall welfare. This study aimed to systematically investigate the interplay between two essential personality traits—boldness and aggression—in Chinese mitten crabs (Eriocheir sinensis [...] Read more.

Territorial crustaceans exhibit a complex set of personality traits and behavioral characteristics that are critical for their survival, growth, and overall welfare. This study aimed to systematically investigate the interplay between two essential personality traits—boldness and aggression—in Chinese mitten crabs (Eriocheir sinensis), during various developmental stages under conditions of crowding stress and food odor stimuli. Using behavioral assays, we found that adults displayed significantly higher levels of boldness and aggression compared to juveniles. Notably, bold adult crabs demonstrated lower aggression than their shy counterparts, revealing a significant negative correlation between boldness and aggression under crowding stress. Furthermore, juvenile and adult crabs exhibited negative correlations between the stability of their boldness and aggression with individual levels of boldness and aggression, respectively. The study also found that while juvenile boldness did not significantly correlate with body size, larger individuals displayed higher boldness under stress, contrasting with adult crabs, where boldness negatively correlated with body size, although aggression levels were unaffected by size; the stability of adult boldness demonstrated a positive correlation with body size under crowding stress. These findings elucidate the intricate dynamics of personality traits in territorial crustaceans, laying the groundwork for future research into their adaptive behavioral strategies in response to environmental cues like group stress and food odors. Full article

(This article belongs to the Section Aquatic Animals)

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10 pages, 1853 KiB

Open AccessArticle

Genetic Diversity in the Diminazene Resistance-Associated P2 Adenosine Transporter-1 (AT-1) Gene of Trypanosoma evansi

by Shoaib Ashraf, Ghulam Yasein, Qasim Ali, Kiran Afshan, Martha Betson, Neil Sargison and Umer Chaudhry

Animals 2025, 15(5), 756; https://doi.org/10.3390/ani15050756 - 6 Mar 2025

Viewed by 457

Abstract

Trypanosomes are parasitic protozoa that cause severe diseases in humans and animals. The most important species of Trypanosmes include Trypanosoma evansi and Trypanosoma brucei gambiense. The most well-known human diseases are sleeping sickness in Africa and Chagas disease in South America. The [...] Read more.

Trypanosomes are parasitic protozoa that cause severe diseases in humans and animals. The most important species of Trypanosmes include Trypanosoma evansi and Trypanosoma brucei gambiense. The most well-known human diseases are sleeping sickness in Africa and Chagas disease in South America. The most identified animal diseases include Nagana in the African tsetse fly belt and Surra in South Asia, North Africa, and the Middle East. Surra is caused by Trypanosoma evansi. Diminazene resistance is an emerging threat caused by T. evansi infecting animals. The underlying mechanism of diminazene resistance is poorly understood. Trypanosoma brucei gambiense causes African sleeping sickness. The development of diminazene resistance in Trypanosoma brucei gambiense is associated with the alterations in the corresponding P2 adenosine transporter-1 (AT-1) gene. In the present study, by extrapolating the findings from Trypanosoma brucei gambiense, we analyzed genetic diversity in the P2 adenosine transporter-1 gene (AT-1) from T. evansi to explore a potential link between the presence of mutations in this locus and diminazene treatment in ruminants. We examined T. evansi-infected blood samples collected from goats, sheep, camels, buffalo, and cattle in seven known endemic regions of the Punjab province of Pakistan. Heterozygosity (H_e) indices indicated a high level of genetic diversity between seven T. evansi field isolates that had resistance-type mutations at codons 178E/S, 239Y/A/E, and 286S/H/I/D/T of the P2 adenosine transporter-1 (AT-1) locus. A low level of genetic diversity was observed in 19 T. evansi field isolates with susceptible-type mutations at codons A178, G181, D239, and N286 of the P2 adenosine transporter-1 (AT-1) locus. Our results on T. evansi warrant further functional studies to explore the relationship between diminazene resistance and the mutations in AT-1. Full article

(This article belongs to the Special Issue Parasite Epidemiology and Molecular Identification in Wild, Domestic, and Companion Animals: 2nd Edition)

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34 pages, 13989 KiB

Open AccessArticle

by Dengyu Li, Kaiqin Zhang, Xiaofeng Xue, Zhanchun Bai, La Yang, Jingjing Qi and Sizhu Suolang

Animals 2025, 15(5), 755; https://doi.org/10.3390/ani15050755 - 6 Mar 2025

Viewed by 539

Abstract

This study aimed to evaluate the therapeutic effect of Terminalia chebula (TC) on Tibetan yak-origin Salmonella-induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and IL-10), and the oxidative stress markers malondialdehyde [...] Read more.

This study aimed to evaluate the therapeutic effect of Terminalia chebula (TC) on Tibetan yak-origin Salmonella-induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and IL-10), and the oxidative stress markers malondialdehyde (MDA), superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), reduced glutathione (GSH-PX), and catalase (CAT) in the serum of mice were measured using ELISA kits. Using microbial diversity sequencing and non-targeted metabolomics detection techniques, the relevant mechanisms of TC treatment in a mouse Salmonella infection model were evaluated. The results showed the following: TC can effectively reduce the diarrhea rate; alleviate weight loss caused by Salmonella invasion; reduce the pro-inflammatory cytokines IL-1β, IL-6, IL-8, and TNF-α in serum; and increase the concentrations of the anti-inflammatory cytokines IL-4 and IL-10. TC can improve the body’s antioxidant levels to heal the damage caused by oxidative stress and lipid peroxidation. The histological section results show that TC can significantly improve gastric and intestinal tissue lesions and has no toxic effects on the liver and kidneys. 16S rRNA and ITS sequencing analysis suggests that Lactobacillus, Enterorhabdus, Alistipes (bacterial community), Lodderomyces, Saccharomyces, and Penicillium (fungal community) may be key functional microbial communities in TC. Non-targeted metabolomics also suggests that the antibacterial treatment of dysentery with chebulic acid may be related to regulation of the Ras signaling pathway, long-term potentiation, the MAPK signaling pathway, metabolic pathways, and gut microbiome composition. Conclusion: TC has clear clinical efficacy in treating bacterial diarrhea, presenting anti-inflammatory and antioxidant effects. Its roles in regulating the gut microbiome and metabolic pathways and products were determined as the main reason for its therapeutic effect in a mouse gastroenteritis model caused by Salmonella infection. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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16 pages, 3532 KiB

Open AccessArticle

Hypothalamus Transcriptome Reveals Key lncRNAs and mRNAs Associated with Fecundity in Goats

by Yingshi Wei, Caiye Zhu, Xiaoyun He and Mingxing Chu

Animals 2025, 15(5), 754; https://doi.org/10.3390/ani15050754 - 6 Mar 2025

Viewed by 479

Abstract

The hypothalamus (hyp) serves as the regulatory hub of the neuroendocrine system, synthesizing and secreting reproductive hormones that modulate estrus, follicular maturation, and embryonic development in goats. This study employed RNA-seq analysis to examine gene expression in the hypothalamic tissue of Yunshang black [...] Read more.

The hypothalamus (hyp) serves as the regulatory hub of the neuroendocrine system, synthesizing and secreting reproductive hormones that modulate estrus, follicular maturation, and embryonic development in goats. This study employed RNA-seq analysis to examine gene expression in the hypothalamic tissue of Yunshang black goats during the luteal phase in goats with high fecundity (LP_HY), during the luteal phase in goats with low fecundity (LP_LY), during the follicular phase in goats with high fecundity (FP_HY), and during the follicular phase in goats with low fecundity (FP_LY). Differential long non-coding RNAs (DE lncRNAs) and differential mRNAs (DE mRNAs) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and the construction of co-expression networks associated with reproduction. As a result, DE lncRNAs (390, 375, 405, and 394) and DE mRNAs (1836, 2047, 2003, and 1963) were identified in the four comparisons, namely FP_LY vs. FP_HY, LP_HY vs. FP_HY, LP_LY vs. FP_LY, and LP_LY vs. LP_HY, respectively. Functional annotations indicated significant enrichment of numerous DE lncRNAs and DE mRNAs in reproduction-related pathways such as the gonadotropin-releasing hormone pathway, the prolactin signaling pathway, the estrogen signaling pathway, the Wnt signaling pathway, oocyte meiosis, and progesterone-mediated oocyte maturation. The co-expression network of lncRNAs and target genes identified the interrelationships between reproduction-related genes such as IGF1, PORCN, PLCB2, MAPK8, PRLR, and CPEB2 with our newly discovered lncRNAs. This study expands the understanding of lncRNAs and mRNAs in goat hypothalamic tissue and provides new insights into molecular mechanisms related to goat reproduction. Full article

(This article belongs to the Section Small Ruminants)

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20 pages, 5682 KiB

Open AccessArticle

Gut Metagenome Reveals the Microbiome Signatures in Tibetan and Black Pigs

by Xue Bai, Yiren Gu, Diyan Li and Mingzhou Li

Animals 2025, 15(5), 753; https://doi.org/10.3390/ani15050753 - 6 Mar 2025

Viewed by 546

Abstract

The harsh conditions of the Qinghai–Tibet Plateau pose significant physiological challenges to local fauna, often resulting in gastrointestinal disorders. However, Tibetan pigs have exhibited remarkable adaptability to the high-altitude stress of the Tibetan Plateau, a phenomenon that remains not fully understood in terms [...] Read more.

The harsh conditions of the Qinghai–Tibet Plateau pose significant physiological challenges to local fauna, often resulting in gastrointestinal disorders. However, Tibetan pigs have exhibited remarkable adaptability to the high-altitude stress of the Tibetan Plateau, a phenomenon that remains not fully understood in terms of their gastrointestinal microbiota. This study collected 57 gastrointestinal tract samples from Tibetan pigs (n = 6) and plain black pigs (n = 6) with comparable genetic backgrounds. Samples from the stomach, jejunum, cecum, colon, and rectum, underwent comprehensive metagenomic analysis to elucidate the gut microbiota-related adaptive mechanisms in Tibetan pigs to the extreme high-altitude environment. A predominance of Pseudomonadota was observed within gut microbiome of Tibetan pigs. Significant differences in the microbial composition were also identified across the tested gastrointestinal segments, with 18 genera and 141 species exhibiting differential abundance. Genera such as Bifidobacterium, Megasphaera, Fusobacterium, and Mitsuokella were significantly more abundant in Tibetan pigs than in their lowland counterparts, suggesting specialized adaptations. Network analysis found greater complexity and modularity in the microbiota of Tibetan pigs compared to black pigs, indicating enhanced ecological stability and adaptability. Functional analysis revealed that the Tibetan pig microbiota was particularly enriched with bacterial species involved in metabolic pathways for propionate and butyrate, key short-chain fatty acids that support energy provision under low-oxygen conditions. The enzymatic profiles of Tibetan pigs, characterized by elevated levels of 4-hydroxybutyrate dehydrogenase and glutaconyl-CoA decarboxylase, highlighted a robust fatty acid metabolism and enhanced tricarboxylic acid cycle activity. In contrast, the gut microbiome of plain black pigs showed a reliance on the succinate pathway, with a reduced butyrate metabolism and lower metabolic flexibility. Taken together, these results demonstrate the crucial role of the gastrointestinal microbiota in the adaptation of Tibetan pigs to high-altitude environments by optimizing carbohydrate metabolism and short-chain fatty acid production for efficient energy utilization. This study not only highlights the metabolic benefits conferred by the gut microbiota of Tibetan pigs in extreme environments, but also advances our understanding of the adaptive gastrointestinal mechanisms in plateau-dwelling animals. These insights lay the foundation for exploring metabolic interventions to support health and performance in high-altitude conditions. Full article

(This article belongs to the Section Pigs)

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14 pages, 3315 KiB

Open AccessArticle

Single-Cell RNA Sequencing Reveals an Atlas of Meihua Pig Testis Cells

by Mao Zhang, Yiming Yan, Guoliang Peng, Shuang Gao, Hongyi Li and Yuan Li

Animals 2025, 15(5), 752; https://doi.org/10.3390/ani15050752 - 5 Mar 2025

Viewed by 697

Abstract

Mammalian spermatogenesis is a complex biological process that is regulated by multiple types of cells. The heterogeneity of these cells poses a challenge for analyzing different cell types at different developmental stages. To characterize the transcriptomic landscape of porcine spermatogenesis and identify potential [...] Read more.

Mammalian spermatogenesis is a complex biological process that is regulated by multiple types of cells. The heterogeneity of these cells poses a challenge for analyzing different cell types at different developmental stages. To characterize the transcriptomic landscape of porcine spermatogenesis and identify potential marker genes for spermatogonia, an unbiased transcriptomic study of spermatogenesis in neonatal and sexually mature six-month-old Meihua pigs was performed using 10× Genomics single-cell RNA sequencing (scRNA-seq). Through the collection of scRNA-seq data from 13,839 cells from Meihua pig testes, three germ cells (spermatogonia, spermatocytes and spermatids) and eight somatic cells (Sertoli cells, Leydig cells, myoid/stromal cells, endothelial cells, T cells/macrophages and erythroblasts) were identified. Pseudo-timing analysis showed that myoid cells and stromal cells originated from common progenitors in Meihua pigs. Functional enrichment analysis revealed that the differentially expressed genes (DEGs) in testicular somatic cells were enriched in the pathways of Ribosome, Oxidative phosphorylation, Protein processing in endoplasmic reticulum, Retrograde endocannabinoid signaling, Cellular senescence and Insulin signaling. Meanwhile, in the three different germ cells, except for pathways which were the same as the first three pathways for somatic cells, DEGs were also enriched in the Spliceosome, Cell cycle, Autophagy and Mitophagy pathways. Furthermore, the candidate marker gene TKTL1 in spermatogonia was identified using immunohistochemistry and immunofluorescence. In conclusion, we collected transcription datasets and constructed single-cell developmental maps of germ cells and somatic cells during the testicular development of Meihua pigs, which provided new insights into the spermatogenesis of Meihua pigs and the development of various types of cells in their testes. Full article

(This article belongs to the Section Animal Reproduction)

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29 pages, 4582 KiB

Open AccessFeature PaperReview

A Literature Review on Equine Bedding: Impacts on Horse and Human Welfare, Health, and the Environment

by Naod Thomas Masebo, Beatrice Benedetti, Maria Mountricha, Leonie Lee and Barbara Padalino

Animals 2025, 15(5), 751; https://doi.org/10.3390/ani15050751 - 5 Mar 2025

Viewed by 1714

Abstract

Bedding is an important component of equine accommodation management. Choosing the right bedding is important for stable management and its selection may include considerations such as the sourcing of the material, the capital investment and ongoing costs, delivery, storage, installation, ongoing labour and [...] Read more.

Bedding is an important component of equine accommodation management. Choosing the right bedding is important for stable management and its selection may include considerations such as the sourcing of the material, the capital investment and ongoing costs, delivery, storage, installation, ongoing labour and maintenance, removal and disposal. Furthermore, it is crucial that the consequences for the health and welfare of horses and humans and the impact on the environment should also be considered. This review aimed to outline the advantages and disadvantages of different horse bedding types, focusing on their effects on the well-being of horses, humans, and the environment. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) technique was used as the methodology for this review. The search was performed in Scopus and Web of Science bibliometric databases and a total of 176 records were screened reading the title and the abstract. After screening, 58 records were retained and another 19 records were identified using their reference lists (i.e., snowballing). Therefore, a total of 77 records were considered. Straw and wood shavings were the most commonly used and studied bedding materials, while research on alternative options remains limited. Straw is identified as horses’ preferred option, while shavings appear to be the easiest to clean, making them the preferred choice for stable workers. The parameters to consider when choosing the bedding most fit for purpose are many and their attributes differ across the various bedding types. This review has compared all the bedding types within the research literature to determine the best overall option using the research-based evidence. Each bedding type offers unique benefits and drawbacks summarised in a user-friendly table. Stable managers must consider and evaluate them to suit their specific needs, including the health and welfare of each horse and the husbandry system involved. Our findings may, therefore, be useful in the decision-making process of equine industry members. Full article

(This article belongs to the Section Equids)

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23 pages, 1000 KiB

Open AccessArticle

Optimization of Split Feeding Strategy for Laying Hens Through a Response Surface Model

by Nasima Akter, Thi Hiep Dao, Tamsyn M. Crowley and Amy F. Moss

Animals 2025, 15(5), 750; https://doi.org/10.3390/ani15050750 - 5 Mar 2025

Cited by 2 | Viewed by 787

Abstract

Laying hens’ metabolism goes through a cyclic process to produce eggs, which requires higher dietary protein and energy in the morning (AM) and higher calcium (Ca) in the afternoon/evening (PM) than the rest of the day. Therefore, poultry scientists are trying to adopt [...] Read more.

Laying hens’ metabolism goes through a cyclic process to produce eggs, which requires higher dietary protein and energy in the morning (AM) and higher calcium (Ca) in the afternoon/evening (PM) than the rest of the day. Therefore, poultry scientists are trying to adopt a new feeding strategy called AM/PM or split feeding to precisely meet hen’s requirements more effectively than conventional methods. A 10-week cage layer trial was carried out via a Box–Behnken response surface design to identify the optimal amount of protein, energy, and calcium of the AM/PM diets. There were 13 test treatments with three levels of crude protein (19.6%/18.4%, 20.3%/17.7%, 21%/17%), calcium (3.3%/4.9%, 2.5%/5.7%, 1.6%/6.6%), and apparent metabolizable energy (AME) (12 MJ/kg/11.2 MJ/kg, 12.4 MJ/kg/10.8 MJ/kg, 12.8 MJ/kg/10.4 MJ/kg) for AM/PM diets respectively and a control treatment with industry baseline (CP-19%, Ca-4.1% and ME 11.6 MJ/kg). These are the calculated values of nutrients on a dry matter basis. A total of 364 hens were randomly distributed into 2 dietary treatments where each treatment had 13 replicates (2 hens per replicate cage, 26 hens per treatment). AM and PM diets were swapped out at approximately 8 am and 4 pm each day. Egg production and hen performance were measured daily and weekly, respectively, with egg quality, serum Ca, and nutrient digestibility measured at week 10. AM:PM intake and feed cost were calculated for each treatment. The optimal FCR, feed cost, and AM:PM intake were used to determine Ca, CP, and AME levels. The result showed that 6 out of 13 of our test treatments gave improved FCR compared to the control treatment (p = 0.017). Dietary treatments did not affect overall hen weight and serum Ca and egg quality at week 10, except for the lower yolk color score in the control treatment (p = 0.002). Hens in the experimental treatment, with calcium levels of 1.6% and 6.6%, crude protein (CP) levels of 19.6% and 18.4%, and AME content of 12.4 MJ/kg and 10.8 MJ/kg in the AM and PM diet, respectively, showed the highest apparent protein digestibility (56.6%) compared to the control group (p < 0.05). Similarly, hens receiving a treatment containing calcium at 3.3% and 4.9%, CP at 21% and 17%, and AME at 12.4 MJ/kg and 10.8 MJ/kg in the AM and PM diet, respectively, achieved the highest calcium digestibility (62.13%), while the control treatment yielded the lowest calcium digestibility (p < 0.05). After analyzing the data using the Box–Behnken response surface methodology, we found that (21/17)% CP, (3.3/4.9)% Ca, and (12/11.12) MJ/kg energy in the AM/PM diet gave the optimum performance in terms of lower feed cost and better feed efficiency. The data of AM:PM intake demonstrate that selective feeding occurs in between treatments (p < 0.001) and the degree of selection depends on the difference between the level of nutrients in AM and PM diet. This study revealed that when optimized, AM/PM feeding improves feed efficiency and egg quality of laying hens. Full article

(This article belongs to the Section Poultry)

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22 pages, 5430 KiB

Open AccessArticle

Multi-Omics Analysis Reveals the Negative Effects of High-Concentrate Diets on the Colonic Epithelium of Dumont Lambs

by Shufang Li, Hairong Wang, Boyang Li, Henan Lu, Jianxin Zhao, Aiwu Gao, Yawen An, Jinli Yang and Tian Ma

Animals 2025, 15(5), 749; https://doi.org/10.3390/ani15050749 - 5 Mar 2025

Viewed by 638

Abstract

Feeding HC diets has been found to induce metabolic dysregulation in the colon. However, the mechanisms by which changes in colonic flora and metabolites damage the colonic epithelium are poorly studied. Therefore, the present experiment used a multi-omics technique to investigate the mechanism [...] Read more.

Feeding HC diets has been found to induce metabolic dysregulation in the colon. However, the mechanisms by which changes in colonic flora and metabolites damage the colonic epithelium are poorly studied. Therefore, the present experiment used a multi-omics technique to investigate the mechanism of colonic injury induced by high-concentrate diets in lambs. Twelve male Dumont lambs were randomly split into two groups: a low-concentrate diet (LC = concentrate/forage = 30:70) group and a high-concentrate diet (HC = concentrate/forage = 70:30) group. The results showed that the HC group presented significantly increased lipopolysaccharide (LPS) concentrations in the colonic epithelium and significantly decreased serum total cholesterol (TC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels (p < 0.05), which led to cavities and inflammatory cell infiltration in the colonic epithelium. The HC group had significantly lower pH and less VFAs in colon contents, as well as a significantly increased abundance of bacteria of the genera [Eubacterium]_coprostanoligenes_group, Rikenellaceae_RC9_gut_group, Treponema, Clostridia_UCG-014, Alistipes, Ruminococcus, Christensenellaceae_R-7_group, UCG-002, Bacteroidales_RF16_group and Lachnospiraceae_AC2044_group compared to the LC diet group. These microorganisms significantly increased the level of metabolites of cholic acid, chenodeoxycholic acid, LysoPA (P-16:0/0:0), methapyrilene, and fusaric acid. A transcriptome analysis showed that cytokine–cytokine receptor interaction, glutathione metabolism, and the peroxisome signaling pathway were downregulated in the colon epithelium of the lambs fed the HC diet. Therefore, the HC diet caused epithelial inflammation and oxidative damage by affecting the interaction between the microbial flora of the colon and metabolites and the host epithelium, which eventually disrupted colon homeostasis and had a negative impact on sheep health. Full article

(This article belongs to the Topic Precision Feeding and Management of Farm Animals, 3rd Edition)

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27 pages, 381 KiB

Open AccessReview

Potential Genetic Markers Associated with Environmental Adaptability in Herbivorous Livestock

by Xiaotong Liu, Yongdong Peng, Xinhao Zhang, Wenting Chen, Yinghui Chen, Lin Wei, Qifei Zhu, Muhammad Zahoor Khan and Changfa Wang

Animals 2025, 15(5), 748; https://doi.org/10.3390/ani15050748 - 5 Mar 2025

Viewed by 651

Abstract

Herbivorous livestock, such as cattle, sheep, goats, horses, and donkeys, play a crucial role in agricultural production and possess remarkable resilience to extreme environmental conditions, driven by complex genetic mechanisms. Recent advancements in high-throughput sequencing, genome assembly, and environmental data integration have enabled [...] Read more.

Herbivorous livestock, such as cattle, sheep, goats, horses, and donkeys, play a crucial role in agricultural production and possess remarkable resilience to extreme environmental conditions, driven by complex genetic mechanisms. Recent advancements in high-throughput sequencing, genome assembly, and environmental data integration have enabled a deeper understanding of the genetic basis of their environmental adaptation. This review identifies key genes associated with high-altitude, heat, cold, and drought adaptation, providing insights into the molecular mechanisms underlying these traits. By elucidating these genetic adaptations, our study aims to support conservation efforts, inform selective breeding programs, and enhance agricultural productivity, ultimately contributing to sustainable livestock farming and economic benefits for farmers. Full article

(This article belongs to the Special Issue Genetic Research for Improving Livestock Heat Stress Resistance)

16 pages, 2502 KiB

Open AccessArticle

Consequences of Domestication on Gut Microbiome: A Comparative Analysis Between Wild Boars and Domestic Pigs

by Da-Yun Bae, Sung-Hyun Moon, Taek Geun Lee, Young-Seung Ko, Yun-Chae Cho, Hamin Kang, Chan-Soo Park, Jung-Sun Kang, Yeonsu Oh and Ho-Seong Cho

Animals 2025, 15(5), 747; https://doi.org/10.3390/ani15050747 - 5 Mar 2025

Cited by 1 | Viewed by 587

Abstract

The gut microbiota plays a vital role in the physiological functions and health of animals. Domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa scrofa), which share a common ancestor, present a unique model for exploring the evolutionary [...] Read more.

The gut microbiota plays a vital role in the physiological functions and health of animals. Domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa scrofa), which share a common ancestor, present a unique model for exploring the evolutionary changes in gut microbiota due to domestication. Comparing the gut microbiota of these two groups provides a unique opportunity to identify microorganisms that may have been lost or altered during domestication. This study employed 16S rRNA sequencing to compare the gut microbiota compositions of domestic pigs and wild boars, analyzing 69 fecal samples from both groups. Our results indicate significant differences in the taxonomic profiles of the gut microbiota between the two groups. Specifically, the genera Bifidobacterium and Lactobacillus, were found in higher abundance in wild boars. In contrast, domestic pigs exhibited a decreased variety of these potentially health-enhancing bacteria, likely due to factors such as simplified diets and antibiotic use. Functional gene analysis revealed that pathways related to carbohydrate metabolism, lipid metabolism, immune response, and environmental adaptability were more enriched in wild boars. These findings demonstrate significant differences in the gut microbiota composition between domestic pigs and wild boars, underscoring the impact of domestication on microbial diversity. Further research is necessary to explore the potential of transferring beneficial microbes from wild to domestic populations. Full article

(This article belongs to the Special Issue Disease and Health in Free-Ranging and Captive Wildlife: Second Edition)

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18 pages, 1588 KiB

Open AccessReview

A Comprehensive Overview of Respiratory Compliance in Dogs Under General Anesthesia: Clinical Factors and Future Perspectives

by Tingfeng Xu, Ahmed S. Mandour, Kazumi Shimada, Lina Hamabe and Ryou Tanaka

Animals 2025, 15(5), 746; https://doi.org/10.3390/ani15050746 - 5 Mar 2025

Viewed by 660

Abstract

Respiratory compliance reflects the ability of the lungs and chest wall to expand in response to increases in pressure. In this review, relevant studies were selected through a comprehensive literature search with the aim of summarizing and generalizing them to describe the relevant [...] Read more.

Respiratory compliance reflects the ability of the lungs and chest wall to expand in response to increases in pressure. In this review, relevant studies were selected through a comprehensive literature search with the aim of summarizing and generalizing them to describe the relevant factors that may be present in veterinary clinical practice and affect respiratory compliance in dogs. Individual factors, including breeds, disease background, drugs administered, and especially surgical procedures, can result in alterations to respiratory compliance due to their impact on the respiratory system in dogs. Despite its potential clinical utility, such as in anesthesia monitoring, respiratory compliance remains underutilized in veterinary medicine, and further research is necessary to support its future clinical applications. Full article

(This article belongs to the Special Issue Advances in Veterinary Surgical, Anesthetic, and Patient Monitoring)

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15 pages, 3160 KiB

Open AccessArticle

Genomic Insights into the Population Genetics and Adaptive Evolution of Yellow Seabream (Acanthopagrus latus) with Whole-Genome Resequencing

by Yuan Li, Jingyu Yang, Yan Fang, Ran Zhang, Zizi Cai, Binbin Shan, Xing Miao, Longshan Lin, Puqing Song and Jing Zhang

Animals 2025, 15(5), 745; https://doi.org/10.3390/ani15050745 - 5 Mar 2025

Viewed by 475

Abstract

Yellow seabream (Acanthopagrus latus), a species of significant economic importance, predominantly inhabits the warm waters of the Indo-Western Pacific. While previous studies have explored the genetic diversity of A. latus using microsatellites and other nuclear markers, a comprehensive understanding of its [...] Read more.

Yellow seabream (Acanthopagrus latus), a species of significant economic importance, predominantly inhabits the warm waters of the Indo-Western Pacific. While previous studies have explored the genetic diversity of A. latus using microsatellites and other nuclear markers, a comprehensive understanding of its genetic characteristics and adaptive evolution using whole-genome resequencing (WGR) remains limited. In this study, we collected 60 individuals from six distinct geographic locations and performed WGR, achieving an average sequencing depth of 12.59×, which resulted in the identification of 19,488,059 high-quality single-nucleotide polymorphisms (SNPs). The nucleotide polymorphism (π_θ) across all populations was consistent, ranging from 0.003042 to 0.003155, indicating low genetic differentiation among populations. Comparative analyses revealed that populations other than that in Xiamen (XM) have undergone adaptive evolution, potentially linked to traits such as growth and development, feeding, immunity, and movement. This study explores the population genetics and adaptive evolutionary patterns of Acanthopagrus latus at the genomic level, providing an essential foundation for the conservation and management of this economically important species in the future. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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16 pages, 1042 KiB

Open AccessArticle

Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers

by Doungnapa Promket, Jennarong Kammongkun, Jiranan Insee, Wootichai Kenchaiwong, Khanitta Pengmeesri, Thassawan Somchan and Wuttigrai Boonkum

Animals 2025, 15(5), 744; https://doi.org/10.3390/ani15050744 - 5 Mar 2025

Viewed by 464

Abstract

This study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total [...] Read more.

This study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total of 117 chickens, including three Thai native breeds and commercial laying hens, were analyzed. The phenotypic traits measured included age at first egg production (AFEP), first egg weight (FEW), egg weight at 9 months (EW_9M) and 12 months (EW_12M) of egg production period, number of eggs at 9 months (NE_9M) and 12 months (NE_12M) of egg production period, number of eggs per month (EperM), and egg mass (EMs). The NPY gene was sequenced to examine the association between these traits and specific genotypes. The results showed that commercial laying hens (LC) significantly outperformed native breeds in overall egg production. Among the native breeds, Pradu Hang Dum (PH) demonstrated the earliest laying age and the highest cumulative egg production. Genetic diversity analysis revealed moderate heterozygosity levels (PIC = 0.22 to 0.50, He = 0.26 to 0.50). Specific SNP loci (SNP1, SNP2, SNP3, SNP4, SNP5, and SNP6) were found to be associated with key egg production traits, such as AFEP, FEW, EW_9M, EW_12M, NE_9M, NE_12M, EperM, and EMs. These findings highlight the potential of using genetic markers for improving egg production traits in Thai native chickens. By incorporating marker-assisted selection into breeding programs, this research supports the development of more efficient and sustainable poultry farming practices, particularly for local breeds. This study also underscores the importance of preserving genetic diversity while enhancing productivity, ensuring the long-term sustainability of native chicken populations. Full article

(This article belongs to the Special Issue Genetics and Breeding Advances in Poultry Health and Production: 2nd Edition)

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18 pages, 9776 KiB

Open AccessArticle

Papillary and Callous Scales in the Integument of Agamid Lizards (Agamidae, Sauria) as a Phenomenon of Extraordinary Development of the Corneous Layers

by Tatjana N. Dujsebayeva, Natalia B. Ananjeva, Nasrullah Rastegar-Pouyani, Awadh M. Al-Johany and Daniel A. Melnikov

Animals 2025, 15(5), 743; https://doi.org/10.3390/ani15050743 - 5 Mar 2025

Viewed by 377

Abstract

Scaled integument of six species of the genus Acanthocercus and Laudakia nupta, family Agamidae was studied using light and scanning electron microscopy. Gross observation revealed the presence of two types of modified scales in the males. The enlarged scales covered with an [...] Read more.

Scaled integument of six species of the genus Acanthocercus and Laudakia nupta, family Agamidae was studied using light and scanning electron microscopy. Gross observation revealed the presence of two types of modified scales in the males. The enlarged scales covered with an extremely thick β-corneous layer were detected in the dorsal and ventral surfaces of the tail base and on the palmar and plantar limb surfaces of all species. After detachment of the β-layer, the surface of such scales was covered with high papillae (“papillary scales”). The callous scales were found in the precloacal region of Acanthocercus species and in both precloacal and mid-ventral regions of L. nupta. Modified scales were found in some females and subadut specimens, and absent in juveniles. A prominent papillary layer characterized the dermis of both scale types. It was assumed that well-developed dermal papillae in such scales expended the total surface area of stratum germinativum and created a pool of proliferated cells in the interpapillary loops to increase the production of differentiating keratinocytes. These processes were undoubtedly associated with the formation of a thick and resistant corneous layer that distinguished both types of scales. Functional role, a taxonomic value, and analogies with normal and pathological epidermis in birds and mammals are discussed. Full article

(This article belongs to the Section Herpetology)

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19 pages, 4679 KiB

Open AccessArticle

Effect of Alligator Weed (Alternanthera philoxeroides) Supplementation on Production Performance, Immune Response and Antioxidant Function of Improved Rural Chicken

by Kekungu-u Puro, Sayed Nabil Abedin, Zakir Hussain, Jaredth B. M. Wankhar, Sunil Doley, Chubasenla Aochen, Burhan Uddin Choudhury, Mahak Singh, Rahul Katiyar and Sourabh Deori

Animals 2025, 15(5), 742; https://doi.org/10.3390/ani15050742 - 5 Mar 2025

Viewed by 591

Abstract

Alligator weed (AW; Alternanthera philoxeroides) can have severe ecological and economic consequences by impacting water quality, flow and the growth of native flora and fauna. Plants, both edible and inedible, contain phenolics, compounds that can serve as antioxidants. Using this background information, [...] Read more.

Alligator weed (AW; Alternanthera philoxeroides) can have severe ecological and economic consequences by impacting water quality, flow and the growth of native flora and fauna. Plants, both edible and inedible, contain phenolics, compounds that can serve as antioxidants. Using this background information, the study aimed to explore the possible antioxidative nature of AW by incorporating it as a supplement on production performance, gene expression, and antioxidant levels during the summer and winter seasons in improved-variety rural chicks. A total of 400 improved-variety Vanaraja chicks (35 days old), were used in each of the two seasons (summer and winter), making a combined total of 800 birds (n = 400 for each summer and winter season). The chicks were subjected to four experimental dietary treatments over a 35-day period during both seasons. The experimental diet consisted of the following: a control diet without any supplements (C); basal diet + 1% AW (T₁); basal diet + 2% AW(T₂); and basal diet + 4% AW(T₃). The production performance, cytokine gene expression (IFN-γ, IL-1β, IL-6, IL-12 and iNOS) and serum antioxidants, viz. catalase (CAT) and superoxide dismutase (SOD), were evaluated. The results indicated that body weight, average body weight gain and weekly feed intake in the T₁ group was significantly (p < 0.05) higher as compared to the other groups. The FCR in group T₁ was significantly (p < 0.05) lower during winter than in summer. A significant (p < 0.001) upregulation in the expression of IL-6, IL-1β and IL-12 in T₁ as compared to the other groups was reported. IFN-γ, IL-1β, IL-6 and iNOS were significantly (p < 0.001) upregulated in winter. SOD and CAT activity was significantly (p < 0.001) higher in T₁ compared to C, and both were significantly (p < 0.05) higher during winter than in summer. The results suggested that AW has the potential to mitigate the consequences of cold stress on growth, immune response, and antioxidant function during winter. We propose adding 1% AW, which can possibly function as an antioxidant, to the diet of chicks to enhance their production performance and immunity levels. Full article

(This article belongs to the Special Issue Sustainable Poultry Production: Physiology and Nutrition (Second Edition))

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12 pages, 581 KiB

Open AccessBrief Report

Morphological and Genetic Assessments of Coyote Diet in Qualla Boundary, North Carolina, Show Interaction with Humans

by Caitlin Miller, Donald Linzey and Eric Hallerman

Animals 2025, 15(5), 741; https://doi.org/10.3390/ani15050741 - 5 Mar 2025

Viewed by 480

Abstract

Throughout the 20th century, coyotes (Canis latrans) expanded from their historical geographic range west of the Mississippi River to a current range of almost all of North America. Over the course of this expansion, coyotes have demonstrated diverse and variable omnivorous [...] Read more.

Throughout the 20th century, coyotes (Canis latrans) expanded from their historical geographic range west of the Mississippi River to a current range of almost all of North America. Over the course of this expansion, coyotes have demonstrated diverse and variable omnivorous diets that change with the food resources available. This study examined the stomach contents of 25 coyotes in an area where they are relatively new, the Qualla Boundary in North Carolina, to better understand the diets of coyotes in this area. A combination of morphological identification and DNA barcoding was used to characterize the stomach contents of coyotes. Both plant and animal material were identified from anthropogenic and natural sources, the latter including native mammals. This study provides one example of the breadth and flexibility of coyote diets and helps build an understanding of how coyotes can adapt to new conditions. Full article

(This article belongs to the Special Issue Ecology and Conservation of Large Carnivores)

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12 pages, 4706 KiB

Open AccessArticle

Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors

by Peeravit Sumpavong, Sarawan Kaewmongkol and Gunn Kaewmongkol

Animals 2025, 15(5), 740; https://doi.org/10.3390/ani15050740 - 5 Mar 2025

Viewed by 485

Abstract

A conventional PCR (cPCR) remains an effective molecular technique for the diagnosis of canine monocytic ehrlichiosis. However, agarose gel electrophoresis requires additional time after thermal cycling. In the present study, we developed a PCR-based lateral flow biosensor (PCR-LFB) to detect Ehrlichia canis ( [...] Read more.

A conventional PCR (cPCR) remains an effective molecular technique for the diagnosis of canine monocytic ehrlichiosis. However, agarose gel electrophoresis requires additional time after thermal cycling. In the present study, we developed a PCR-based lateral flow biosensor (PCR-LFB) to detect Ehrlichia canis (E. canis). Lateral flow strips allow for the simple and rapid detection of PCR products and provide an alternative to gel electrophoresis. The sensitivity, specificity, and detection limit of PCR-LFB were compared to those of TaqMan probe-based real-time PCRs (qPCRs). The PCR-LFB was performed with 5′ 6-FITC and biotin-labeled primers specific to E. canis, targeting the dsb gene. The detection limit of the PCR-LFB assay was 10⁻⁶ for the target DNA sequence in a 10-fold dilution of the recombinant plasmid, which is 10 times lower than that of qPCR. Among the confirmed qPCR results in the 30 dog samples, false-positive results were not detected by the PCR-LFB. Compared to qPCR, the sensitivity and specificity of PCR-LFB were 63.6% (95% CI; 42.9–80.2%) and 100% (95% CI; 67.5–100%), respectively. The Kappa value of the PCR-LFB is in moderate agreement with the qPCR (κ = 0.483). Perfect agreement (κ = 1) was observed between cPCR and PCR-LFB. Lower cost and shorter time consumption were demonstrated using PCR-LFB. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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13 pages, 2569 KiB

Open AccessCommunication

Occurrence of Ocular Disorders in California Sea Lions Under Human Care: Comparing Freshwater and Saltwater Housing Conditions

by Ingrid Brehm, Silas Herzner, Katrin Baumgartner, Jörg Beckmann, Ralph Simon and Lorenzo von Fersen

Animals 2025, 15(5), 739; https://doi.org/10.3390/ani15050739 - 5 Mar 2025

Viewed by 498

Abstract

California sea lions (Zalophus californianus) are susceptible to various ocular diseases, with some literature suggesting a heightened risk for those kept in freshwater pools as opposed to saltwater. Given the potential implications of housing conditions on animal health, we wanted to [...] Read more.

California sea lions (Zalophus californianus) are susceptible to various ocular diseases, with some literature suggesting a heightened risk for those kept in freshwater pools as opposed to saltwater. Given the potential implications of housing conditions on animal health, we wanted to analyze the ocular health of two distinct groups of California sea lions kept at Nuremberg Zoo, one housed in freshwater and the other in saltwater. Data extracted from the animals’ medical records during a ten-year period were used to compare the incidence of eye conditions and other medical conditions observed. The results revealed no significant difference in the overall occurrence of eye diseases between the two environments. However, a distinct seasonal pattern was noted: California sea lions kept in freshwater exhibited a peak in eye disorders during the summer months, while those in saltwater displayed a more uniform distribution of the occurrence of ocular diseases throughout the year. These findings suggest that sun exposure and water quality are potentially more influential factors in the development of ocular diseases in pinnipeds than salinity. Further studies are necessary to elucidate the underlying mechanisms and optimize care practices for these marine mammals. Full article

(This article belongs to the Section Zoo Animals)

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16 pages, 6807 KiB

Open AccessArticle

Effects of Yeast Culture on Lamb Growth Performance, Rumen Microbiota, and Metabolites

by Jinlong Xu, Xiongxiong Li, Qingshan Fan, Shengguo Zhao and Ting Jiao

Animals 2025, 15(5), 738; https://doi.org/10.3390/ani15050738 - 5 Mar 2025

Viewed by 486

Abstract

The effects of incorporating yeast culture (YC) into pelleted feeds on sheep production and the potential impact on rumen microbial populations, microbial metabolism, and fermentation have not been extensively studied. This study aimed to evaluate the effect of YC on growth performance, rumen [...] Read more.

The effects of incorporating yeast culture (YC) into pelleted feeds on sheep production and the potential impact on rumen microbial populations, microbial metabolism, and fermentation have not been extensively studied. This study aimed to evaluate the effect of YC on growth performance, rumen tissue development, rumen fermentation, and rumen microflora in sheep and to explore the potential microbial mechanisms involved. Fifty healthy 3-month-old male lambs of small-tailed Han sheep, with an average weight of 28.44 ± 0.63 kg, were randomly divided into five groups: control (0% YC), 3% YC, 6% YC, 9% YC, and 12% YC. The pre-feeding period lasted for 15 days, followed by an official feeding period of 60 days. On the last day of the formal feeding period, six lambs that exhibited the best growth performance were randomly selected from the control group and the 9% YC group. These sheep were slaughtered, then the rumen epithelial tissue and rumen contents were collected for the measurement of rumen fermentation, microbial populations, and metabolites. Compared to the control group, the YC-treated groups showed higher daily and final body weight gains, as well as increased levels of propionic acid, butyric acid, and total volatile fatty acids (p < 0.05). YC supplementation also enhanced rumen papilla length and width (p < 0.05). Additionally, YC increased the relative abundance of certain microbial species (p < 0.05). These results suggest that supplementing 9% YC in pelleted diets for small-tailed Han sheep may enhance growth performance and improve the rumen environment. Full article

(This article belongs to the Special Issue Maximizing Fiber Utilization for Sustainable, Efficient Ruminant Production)

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15 pages, 2895 KiB

Open AccessArticle

Classification of FAMACHA© Scores with Support Vector Machine Algorithm from Body Condition Score and Hematological Parameters in Pelibuey Sheep

by Oswaldo Margarito Torres-Chable, Cem Tırınk, Rosa Inés Parra-Cortés, Miguel Ángel Gastelum Delgado, Ignacio Vázquez Martínez, Armando Gomez-Vazquez, Aldenamar Cruz-Hernandez, Enrique Camacho-Pérez, Dany Alejandro Dzib-Cauich, Uğur Şen, Hacer Tüfekci, Lütfi Bayyurt, Hilal Tozlu Çelik, Ömer Faruk Yılmaz and Alfonso J. Chay-Canul

Animals 2025, 15(5), 737; https://doi.org/10.3390/ani15050737 - 4 Mar 2025

Viewed by 706

Abstract

The aim of this study is to evaluate the model performance in the classification of FAMACHA© scores using Support Vector Machines (SVMs) with a focus on the estimation of the FAMACHA© scoring system used for early diagnosis and treatment management of parasitic infections. [...] Read more.

The aim of this study is to evaluate the model performance in the classification of FAMACHA© scores using Support Vector Machines (SVMs) with a focus on the estimation of the FAMACHA© scoring system used for early diagnosis and treatment management of parasitic infections. FAMACHA© scores are a color-based visual assessment system used to determine parasite load in animals, and in this study, the accuracy of the model was investigated. The model’s accuracy rate was analyzed in detail with metrics such as sensitivity, specificity, and positive/negative predictive values. The results showed that the model had high sensitivity and specificity rates for class 1 and class 3, while the performance was relatively low for class 2. These findings not only demonstrate that SVM is an effective method for classifying FAMACHA© scores but also highlight the need for improvement for class 2. In particular, the high accuracy rate (97.26%) and high kappa value (0.9588) of the model indicate that SVM is a reliable tool for FAMACHA© score estimation. In conclusion, this study demonstrates the potential of SVM technology in veterinary epidemiology and provides important information for future applications. These results may contribute to efforts to improve scientific approaches for the management of parasitic infections. Full article

(This article belongs to the Section Animal System and Management)

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28 pages, 5676 KiB

Open AccessArticle

The Use of Cognition by Amphibians Confronting Environmental Change: Examples from the Behavioral Ecology of Crawfish Frogs (Rana areolata)

by Michael J. Lannoo and Rochelle M. Stiles

Animals 2025, 15(5), 736; https://doi.org/10.3390/ani15050736 - 4 Mar 2025

Viewed by 506

Abstract

Amphibian conservation concerns frequently center on the idea of ‘saving’ them, with the underlying assumption they are the passive victims of anthropogenic environmental change. But this approach ignores the physiological, biochemical, and behavioral flexibility amphibians have employed since they first evolved ~365 million [...] Read more.

Amphibian conservation concerns frequently center on the idea of ‘saving’ them, with the underlying assumption they are the passive victims of anthropogenic environmental change. But this approach ignores the physiological, biochemical, and behavioral flexibility amphibians have employed since they first evolved ~365 million years ago. One overlooked advantage amphibians possess in the struggle for survival, and one humans might use in their efforts to conserve them, is their brains share the same blueprint as human brains, which allows them to acquire knowledge and understanding through experiences—in other words, amphibians have cognitive capabilities that assist them in their effort to survive. Here, we use four examples from our work on the behavioral ecology of Crawfish Frogs (Rana areolata) to form hypotheses about how cognition affects amphibian reaction to environmental and social change. The first two examples describe Crawfish Frog responses to seasonality and reproductive status, the third details their reaction to ecological disturbance, and the fourth describes how their response to the same stimulus changes with growth/age. In each example, we detail the neuronal circuitry thought to be involved and hypothesize the role of cognition. We propose that as one component of our fight to conserve amphibians, researchers should consider the full range of anatomical, physiological, biochemical, and behavioral features amphibians themselves employ in their defense, which are features responsible for their historical evolutionary success up until the Anthropocene. Further, we submit that acknowledging amphibians possess cognitive abilities can enrich interpretations of not only behavioral and ecological observations but also of neuroanatomical and neurophysiological results. Full article

(This article belongs to the Special Issue Thermoregulatory and Physiological Adaptations of Reptiles and Amphibians to Changing Environments)

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28 pages, 11026 KiB

Open AccessFeature PaperArticle

Dolphin Pituitary Gland: Immunohistochemistry and Ultrastructural Cell Characterization Following a Novel Anatomical Dissection Protocol and Non-Invasive Imaging (MRI)

by Paula Alonso-Almorox, Alfonso Blanco, Carla Fiorito, Eva Sierra, Cristian Suárez-Santana, Francesco Consolli, Manuel Arbelo, Raiden Grandía Guzmán, Ignacio Molpeceres-Diego, Antonio Fernández Gómez, Javier Almunia, Ayoze Castro-Alonso and Antonio Fernández

Animals 2025, 15(5), 735; https://doi.org/10.3390/ani15050735 - 4 Mar 2025

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Abstract

The pituitary gland regulates essential physiological processes in mammals. Despite its importance, research on its anatomy and ultrastructure in dolphins remains scarce. Using non-invasive imaging technology (MRI) and a novel skull-opening and dissection protocol, this study characterizes the dolphin pituitary through immunohistochemistry (IHC) [...] Read more.

The pituitary gland regulates essential physiological processes in mammals. Despite its importance, research on its anatomy and ultrastructure in dolphins remains scarce. Using non-invasive imaging technology (MRI) and a novel skull-opening and dissection protocol, this study characterizes the dolphin pituitary through immunohistochemistry (IHC) and transmission electron microscopy (TEM). A total of 47 pituitaries were collected from stranded common bottlenose dolphins (Tursiops truncatus). common dolphins (Delphinus delphis), and Atlantic spotted dolphins (Stenella frontalis). as well as from captive common bottlenose dolphins. MRI allowed visualization of the gland’s anatomy and its spatial relationship with the hypothalamus and surrounding structures. A modified skull-opening and pituitary extraction protocol ensured the preservation of the adenohypophysis and neurohypophysis for detailed analysis. Histological, immunohistochemical, and ultrastructural studies confirmed the gland’s structural organization, identifying eight distinct adenohypophyseal cell types: corticotrophs (ACTH), somatotrophs (GH), gonadotrophs (FSH and LH), lactotrophs (LTH), melanotrophs (MSH), thyrotrophs (TSH), follicular cells, and capsular cells. This study presents the first immunolabelling of thyrotrophs in cetacean adenohypophysis and the first detailed ultrastructural characterization of adenohypophyseal cells in cetaceans, providing baseline data for future research. By integrating multidisciplinary techniques, it advances the understanding of dolphin neuroendocrinology and highlights broader implications for cetacean health, welfare, and conservation. Full article

(This article belongs to the Section Wildlife)

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Animals, Volume 15, Issue 5 (March-1 2025) – 152 articles

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