Animals

9 pages, 1188 KiB

Open AccessCase Report

Intranasal Dental Repulsion of a Displaced Cheek Tooth in an Arabian Filly

by Alessandro Spadari, Giuditta Saragoni, Federica Meistro, Maria Virginia Ralletti, Francesca Marzari and Riccardo Rinnovati

Animals 2025, 15(6), 772; https://doi.org/10.3390/ani15060772 (registering DOI) - 8 Mar 2025

Abstract

This case report documents an unusual cheek tooth (CT) displacement in a 3-year-old Arabian filly. The horse was referred to the clinic for loss of appetite and right-sided facial deformity. At admission, an oral inspection was performed, followed by a dental radiographic examination. [...] Read more.

This case report documents an unusual cheek tooth (CT) displacement in a 3-year-old Arabian filly. The horse was referred to the clinic for loss of appetite and right-sided facial deformity. At admission, an oral inspection was performed, followed by a dental radiographic examination. The radiographs showed a very oblique vertical angulation (135°) of the CT 106 compared to the other premolars (107 and 108 CTs) (40°). In consideration of the clinical and radiographic findings, surgical extraction of the 106 CT was indicated. Due to the unique positioning of the displaced tooth, traditional extraction methods such as standing oral extraction or minimally invasive buccotomy were deemed impractical. An innovative intranasal dental repulsion technique was selected to minimize invasiveness while ensuring the possibility of performing a complete oral extraction. The procedure was performed under general anesthesia without requiring skin incisions or a lateral osteotomy of the maxilla. The surgery was successful, with no intra-operative or post-operative complications. This original approach offers a viable alternative for similar cases, reducing the morbidity associated with conventional repulsion techniques. Full article

(This article belongs to the Section Equids)

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27 pages, 1095 KiB

Open AccessArticle

Social Perceptions and Attitudes Towards Free-Roaming Cats and Dogs in Portugal: An Exploratory Study

by Alexandre Azevedo, Filipa Peste, Paloma Linck, João Carvalho, Danielle Crawshaw, Eduardo Ferreira, Rita Tinoco Torres and Victor Bandeira

Animals 2025, 15(6), 771; https://doi.org/10.3390/ani15060771 (registering DOI) - 8 Mar 2025

Abstract

Free-roaming cats and dogs impact biodiversity, public health, and the welfare of other animals. Attitudes towards free-roaming animals can influence their population dynamics and management success. We conducted an online survey to evaluate social perceptions and attitudes towards free-roaming animals among self-selected Portuguese [...] Read more.

Free-roaming cats and dogs impact biodiversity, public health, and the welfare of other animals. Attitudes towards free-roaming animals can influence their population dynamics and management success. We conducted an online survey to evaluate social perceptions and attitudes towards free-roaming animals among self-selected Portuguese residents aged 18 or older with internet access. The survey focused on responsible ownership, perceptions and attitudes, and management practices, and allowed the collection and analysis of 1083 responses (607 for dogs and 476 for cats). Our results identified needs for improvement in pet ownership: increasing pet cat identification, reducing unsupervised outdoor access, and promoting pet dog sterilization. In terms of management strategies, we found strong support for trap–neuter–release, sheltering, sanctions on abandonment, and educational campaigns. We also found limited support for lethal control methods and fear of culling and long-term caging as barriers to reporting free-roaming animals. While our findings are based on a self-selected online sample, they establish a foundation for future research while also offering valuable guidance for policymakers and stakeholders. Full article

(This article belongs to the Special Issue Free-Roaming Cats and Dogs: Ecology, Management and Inter-Species Interactions)

17 pages, 2466 KiB

Open AccessArticle

Genetic Diversity and Population Structure of Largefin Longbarbel Catfish (Hemibagrus macropterus) Inferred by mtDNA and Microsatellite DNA Markers

by Yanling Hou, Huan Ye, Huamei Yue, Junyi Li, Ling Huang, Ziling Qu, Rui Ruan, Danqing Lin, Zhiqiang Liang, Yong Xie and Chuangju Li

Animals 2025, 15(6), 770; https://doi.org/10.3390/ani15060770 (registering DOI) - 8 Mar 2025

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Abstract

The largefin longbarbel catfish (Hemibagrus macropterus), a freshwater species endemic to China with fundamental economic importance, requires investigation into its genetic structure for effective management. In this study, we employed mitochondrial cytochrome b (Cytb) gene sequences and 14 microsatellite [...] Read more.

The largefin longbarbel catfish (Hemibagrus macropterus), a freshwater species endemic to China with fundamental economic importance, requires investigation into its genetic structure for effective management. In this study, we employed mitochondrial cytochrome b (Cytb) gene sequences and 14 microsatellite loci to elucidate the genetic structure of 195 individuals across eight distinct populations. The Cytb analysis revealed a haplotype number (H) of 31, haplotype diversity (Hd) of 0.853, and nucleotide diversity (π) of 0.0127. Population neutrality tests indicated that Tajima’s D (−0.59467) and Fu and Li’s D* (0.56621) were not statistically significant, and the mismatch distribution exhibited a multimodal pattern. Microsatellite analysis revealed that the mean number of alleles (Na), observed heterozygosity (Ho), and polymorphic information content (PIC) across all loci were 18.500, 0.761, and 0.808, respectively. The UPGMA phylogram constructed based on genetic distance identified two distinct clusters, with paired Fst values ranging from 0.108 to 0.138. These results suggest that the largefin longbarbel catfish is in a state of dynamic equilibrium with high genetic diversity. Furthermore, there was significant genetic differentiation between the YB population and the other seven populations, indicating that the population in the upper reaches of the Yangtze River should be managed as a distinct unit. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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20 pages, 2795 KiB

Open AccessArticle

Effects of Feeding Reduced Protein Diets on Milk Quality, Nitrogen Balance and Rumen Microbiota in Lactating Goats

by Runqi Fu, Ye Yu, Yuning Suo, Binlong Fu, Huan Gao, Lin Han and Jing Leng

Animals 2025, 15(6), 769; https://doi.org/10.3390/ani15060769 - 7 Mar 2025

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Abstract

Lowering dietary protein content is one of the effective ways to reduce nitrogen (N) emissions and conserve protein feed resources. However, it is unclear how reducing dietary protein levels affects milk quality and the efficiency of N utilization in lactating goats. It is [...] Read more.

Lowering dietary protein content is one of the effective ways to reduce nitrogen (N) emissions and conserve protein feed resources. However, it is unclear how reducing dietary protein levels affects milk quality and the efficiency of N utilization in lactating goats. It is therefore difficult to determine exactly how much reduction in dietary protein levels is optimal. The objective of this study was to evaluate the effects of low-protein diets on milk quality, N balance and rumen microbiota in lactating goats. A total of 50 lactating goats were enrolled in a completely randomized design and maintained on either a diet with 15.82% protein level as the control group (CON) or reduced protein levels with 13.85% (R2 group), 11.86% (R4 group), 9.84% (R6 group) and 7.85% (R8 group), respectively. The results showed that the dry matter intake, milk yield, fecal and urinary N excretion and utilization efficiency of N of lactating goats decreased linearly with reduced dietary protein levels. Specifically, the milk yield was reduced by the R8 group (p < 0.05). Furthermore, the R8 group reduced the contents of protein, fat and lactose (p < 0.05), but R2 and R4 have no influence (p > 0.05). The R6 group decreased protein content only at the 4th week. Fecal and urinary N excretion and utilization efficiency of N reduced linearly with decreasing dietary protein levels (p < 0.05). The R8 group affected the relative abundance of rumen microbiota including Christensenellaceae_R-7_group, NK4A214_group and UCG-005 (p < 0.05). In conclusion, lowering dietary protein levels decreased milk quality and N excretion by altering rumen microbiota in goats during lactation. This phenomenon was most pronounced when the dietary protein level was reduced by 8 percentage points. Nevertheless, dietary protein levels should not be reduced by more than 6 percentage points to ensure normal performance of the goat during lactation. Full article

(This article belongs to the Section Animal Nutrition)

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11 pages, 596 KiB

Open AccessFeature PaperArticle

Hair Allopregnanolone in Mares and Foals as a Retrospective Biomarker of Predicting Feto-Maternal Well-Being

by Nicola Ellero, Aliai Lanci, Jole Mariella, Robin van den Boom, Alessio Cotticelli, Tanja Peric, Alberto Prandi, Francesca Freccero and Carolina Castagnetti

Animals 2025, 15(6), 768; https://doi.org/10.3390/ani15060768 - 7 Mar 2025

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Abstract

Assay of steroid hormones in hair has become an attractive alternative for studies focusing on the perinatal period in equine medicine. The aim of the present study was to evaluate mares’ and foals’ hair ALLO concentrations and their ratio in relation to clinical [...] Read more.

Assay of steroid hormones in hair has become an attractive alternative for studies focusing on the perinatal period in equine medicine. The aim of the present study was to evaluate mares’ and foals’ hair ALLO concentrations and their ratio in relation to clinical conditions and selected clinical parameters. The 37 mare–foal pairs were categorized into healthy (group H; n = 15) and sick (group S; n = 22) groups. ALLO from hair was measured using a commercial ELISA kit. Foal ALLO and foal/mare ALLO ratio were lower in group S compared to group H (p < 0.001). Moderate positive correlations were found between both the foal ALLO and foal/mare ALLO ratio and the mare’s gestation length (p = 0.003; r = 0.476 and p = 0.002; r = 0.487), between the foal ALLO and foal’s weight (p = 0.042; r = 0.336), and between the foal/mare ALLO ratio and foal’s Apgar score (p = 0.047; r = 0.410). Based on a logistic regression model, a strong relationship (R² = 0.75) emerged between ALLO concentrations and foals’ clinical outcome, with concentrations of the hormone predicting foals’ clinical outcome with high accuracy (86.8%). Decreased foal ALLO and foal/mare ALLO ratio in sick foals appear to be potential biomarkers of prenatal disease toward the end of pregnancy. Full article

(This article belongs to the Section Equids)

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8 pages, 388 KiB

Open AccessArticle

Effects of Oxidative Stress and Antioxidant Activity in Plasma and Uterine Fluid During Early Postpartum on Subsequent Reproductive Performance of Japanese Black Cows

by Yujiro Hagita, Ryotaro Miura, Koumei Shirasuna, Tadaharu Ajito and Hirotaka Matsumoto

Animals 2025, 15(6), 767; https://doi.org/10.3390/ani15060767 - 7 Mar 2025

Viewed by 44

Abstract

This study aimed to investigate the effects of oxidative stress and antioxidant activity in plasma and uterine fluid during early postpartum on the reproductive performance of Japanese Black cows. A total of 17 Japanese Black cows were included in this study. Plasma and [...] Read more.

This study aimed to investigate the effects of oxidative stress and antioxidant activity in plasma and uterine fluid during early postpartum on the reproductive performance of Japanese Black cows. A total of 17 Japanese Black cows were included in this study. Plasma and uterine fluid samples were collected at days 7 and 14 of the estrus cycle (n = 17), between 47–67 days postpartum, and diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were measured. d-ROMs, BAP, and oxidation stress index (OSI: d-ROMs/BAP × 100) were compared between pregnant and nonpregnant cows at first postpartum artificial insemination (AI), cows pregnant less than 120 days postpartum (<120 days postpartum), and cows pregnant more than 120 days postpartum (≥120 days postpartum) regardless of AI number. The uterine fluid OSI on day 7 of the estrus cycle was significantly higher in cows pregnant ≥ 120 days postpartum (p < 0.05). d-ROMs, BAP, and OSI of uterine fluid during early postpartum were useful indicators for determining the subsequent reproductive performance of Japanese Black cows. Full article

(This article belongs to the Section Animal Physiology)

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25 pages, 338 KiB

Open AccessFeature PaperArticle

A Purr-Suasive Case for Sterilization: How Sterilizing Working Cats Supports Dairy Farmers’ Wellbeing, Improves Animal Welfare, and Benefits the Environment

by Caitlin Crawford, Jacquie Rand, Olivia Forge, Vanessa Rohlf, Pauleen Bennett and Rebekah Scotney

Animals 2025, 15(6), 766; https://doi.org/10.3390/ani15060766 - 7 Mar 2025

Viewed by 85

Abstract

Rodents on farms are a substantial issue due to the damage they cause to produce and infrastructure, and the risk they pose to food safety. As a result, farmers need to control rodent populations around farms. In a previous study, farmers indicated a [...] Read more.

Rodents on farms are a substantial issue due to the damage they cause to produce and infrastructure, and the risk they pose to food safety. As a result, farmers need to control rodent populations around farms. In a previous study, farmers indicated a preference for working cats, stating that they are a more efficient, safer, and cheaper alternative to rodenticide baits, and they provide companionship. However, farmers’ options for managing cat populations are often limited to lethal methods because of time and financial constraints. Through semi-structured interviews, this study explored the impacts of lethal cat management methods on farmers, their experience with a free cat sterilization program, and their views on a Barn Cat Program, whereby healthy stray cats would be vaccinated, sterilized, and made available to farmers. Our results indicated that farmers had a relationship with the cats on their farms and that using lethal methods to manage these cats had a negative impact on farmers’ wellbeing. Farmers supported the free cat sterilization program, as it reduced the cat population and their impact on wildlife, improved cat behavior and welfare, and benefited farmers. Farmers also generally supported a Barn Cat Program and gave suggestions on how best to promote it to other farmers. Funding should be provided for cat sterilization programs, as they support farmers, who value cats for rodent control and companionship, improve cat behavior and welfare, and reduce working cat populations and their impact on wildlife. Full article

(This article belongs to the Section Human-Animal Interactions, Animal Behaviour and Emotion)

12 pages, 1033 KiB

Open AccessArticle

In Vitro Assessment of the Effectiveness of Mineral Adsorbents in Sequestering Boar Taint Compounds

by Sanghyuk Park and James Squires

Animals 2025, 15(6), 765; https://doi.org/10.3390/ani15060765 - 7 Mar 2025

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Abstract

The utility of four mineral adsorbents as potential feed additives to bind the boar taint compounds, androstenone and skatole, was assessed with an in vitro system. The adsorbents were bentonite (BNT), diatomaceous earth (DE), spent filter aid (SFA) and hydrated sodium–calcium aluminosilicate (HSCAS), [...] Read more.

The utility of four mineral adsorbents as potential feed additives to bind the boar taint compounds, androstenone and skatole, was assessed with an in vitro system. The adsorbents were bentonite (BNT), diatomaceous earth (DE), spent filter aid (SFA) and hydrated sodium–calcium aluminosilicate (HSCAS), with activated charcoal (AC) as a positive control. The binding capacity (B_max) and binding affinity (K) of androstenone (AND), estrone (E1), estrone sulfate (E1S), and skatole were estimated using the modified Michaelis–Menten kinetics. The Langmuir and Freundlich isotherm models were also used to assess the adsorption behaviour. The B_max values with AND were 77.7 ± 1.12%, 71.9 ± 1.93%, 55.0 ± 7.85%, and 69.5 ± 1.39% for BNT, DE, SFA, and HSCAS, respectively, with no differences in the binding affinity K (p > 0.05). All the mineral adsorbents had very low binding with E1S. SFA bound skatole with a B_max of 89.9 ± 1.09%, while the B_max values for skatole binding by BNT, DE and HCAS were approximately 15%. Most adsorbent–adsorbate complexes fit best with the Freundlich isotherm model. We conclude that all four mineral adsorbents bound androstenone, but not E1S, and only SFA effectively bound skatole. This suggests that SFA may act as a selective dietary binding agent to control boar taint, but further research using animal models is needed to explore the utility and selectivity of these adsorbents as feed additives to control boar taint. Full article

(This article belongs to the Special Issue Impact of Genetics and Feeding on Growth Performance of Pigs)

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Animals, Volume 15, Issue 6 (March-2 2025) – 8 articles

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