Combined Metabolome and Transcriptome Analysis Revealed the Accumulation of Anthocyanins in Grape Berry (Vitis vinifera L.) under High-Temperature Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Chromaticity Values of Grape Peel
2.3. Anthocyanin and Procyanidin Content in the Peel
2.4. Metabolite Extraction and Data Analysis
2.5. Total RNA Extraction and Transcriptome Sequencing
2.6. Validation of Gene Expression by qRT-PCR
2.7. Data Analysis
3. Results
3.1. High-Temperature Stress Affected the Berry Coloring and Anthocyanin and Procyanidin Contents in Grape
3.2. Targeted Metabolomic Response of XH and FL Grape Peels to HT Stress
3.3. Transcriptional Responses to HT Stress of XH and FL Grapes
3.4. Analysis of DEGs of XH and FL Grapes
3.5. Functional Enrichment Analysis of DEGs of XH and FL Grapes
3.6. Transcription Family (TF) Genes Respond to HT Stress of XH and FL Grapes
3.7. Key DEGs and DAMs Associated with Production of Anthocyanins in XH and FL Grapes after HT
3.8. Anthocyanin, Procyanidin and DEG Co-Expression Networks during Grape Peel Development
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Dou, F.; Phillip, F.O.; Liu, H. Combined Metabolome and Transcriptome Analysis Revealed the Accumulation of Anthocyanins in Grape Berry (Vitis vinifera L.) under High-Temperature Stress. Plants 2024, 13, 2394. https://doi.org/10.3390/plants13172394
Dou F, Phillip FO, Liu H. Combined Metabolome and Transcriptome Analysis Revealed the Accumulation of Anthocyanins in Grape Berry (Vitis vinifera L.) under High-Temperature Stress. Plants. 2024; 13(17):2394. https://doi.org/10.3390/plants13172394
Chicago/Turabian StyleDou, Feifei, Fesobi Olumide Phillip, and Huaifeng Liu. 2024. "Combined Metabolome and Transcriptome Analysis Revealed the Accumulation of Anthocyanins in Grape Berry (Vitis vinifera L.) under High-Temperature Stress" Plants 13, no. 17: 2394. https://doi.org/10.3390/plants13172394