Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana
Abstract
:1. Introduction
2. Results
2.1. MeJA Treatment Improved Abiotic Stress Resistance in Banana
2.2. Sample Preparation and RNA Quality Assessment
2.3. Identification and Analysis of Differentially Expressed Genes
2.4. Gene Ontology Enrichment Analysis of the DEGs
2.5. Kyoto Encyclopedia of Genes and Genomes Enrichment Analysis of DEGs
2.6. Regulatory Mechanisms in Leaves Altered upon MeJA Treatment
2.7. Regulatory Mechanisms in Roots Altered upon MeJA Treatment
2.8. Linoleic Acid Metabolism Showed Tissue-Specific Enrichment
2.9. Analysis of the Promoter Region of the Genes Involved in Linoleic Acid Metabolism
2.10. Verification of the Expression Patterns of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Measurement of CAT, SOD, and POD Activity and MDA Content
4.3. RNA-Seq and Expression Analysis
4.4. GO and KEGG Enrichment Analysis of DEGs
4.5. Promoter Sequence Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, J.; Tang, L.; Qiao, F.; Liu, J.; Li, X. Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana. Plants 2024, 13, 712. https://doi.org/10.3390/plants13050712
Yu J, Tang L, Qiao F, Liu J, Li X. Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana. Plants. 2024; 13(5):712. https://doi.org/10.3390/plants13050712
Chicago/Turabian StyleYu, Jiaxuan, Lu Tang, Fei Qiao, Juhua Liu, and Xinguo Li. 2024. "Physiological and Transcriptomic Analyses Reveal the Mechanisms Underlying Methyl Jasmonate-Induced Mannitol Stress Resistance in Banana" Plants 13, no. 5: 712. https://doi.org/10.3390/plants13050712