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Methods and Protocols, Volume 5, Issue 6

2022 December - 17 articles

Cover Story: During the first wave of COVID-19, there was uncertainty about the frequency of secondary bacterial infection requiring antibiotic therapy and concern about overuse of antibiotics. Procalcitonin (PCT), an inflammatory marker used to guide antibiotic treatment, was widely adopted early in the pandemic, with some evidence that it reduced antibiotic use. This protocol is for a retrospective, cohort study to compare antibiotic use and patient outcomes in those who did and did not have a baseline PCT test when COVID-19 was diagnosed. Clinical data from patients with a positive COVID-19 test 1/2/20-30/6/20 and admitted to 11 acute hospital Trusts and Health Boards in England and Wales will be eligible. Primary outcome is early antibiotic use (first 7 days); secondary outcomes include total antibiotic use, length of stay and mortality. View this paper
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Articles (17)

  • Study Protocol
  • Open Access
1 Citations
3,800 Views
9 Pages

Association of Ultrasonography and Health Education during Antenatal Visits among Pregnant Women to Reduce Unnecessary Caesarean Section in Bangladesh: Study Protocol for a Cluster Randomized Control Trial

  • Habiba Shirin,
  • Michiko Moriyama,
  • K.A.T.M. Ehsanul Huq,
  • Md Moshiur Rahman,
  • Sumaya Binte Masud,
  • Rowshan Ara Begum,
  • Kamrunnahar Misty and
  • Mohammad Delwer Hossain Hawlader

17 December 2022

The rate of caesarean section (C/S) has been increasing globally. The proposed study aims to evaluate the effects of ultrasonography (USG) and health education in reducing unnecessary C/S among pregnant women. The secondary objectives are to increase...

  • Article
  • Open Access
6 Citations
3,353 Views
14 Pages

Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bactericidal Activity of Human Sera against Nontyphoidal Salmonella

  • Maria Grazia Aruta,
  • Daniele De Simone,
  • Helen Dale,
  • Esmelda Chirwa,
  • Innocent Kadwala,
  • Maurice Mbewe,
  • Happy Banda,
  • Melita Gordon,
  • Mariagrazia Pizza and
  • on behalf of the Vacc-iNTS Consortium Collaborators
  • + 4 authors

16 December 2022

Salmonella Typhimurium and Salmonella Enteritidis are leading causative agents of invasive nontyphoidal Salmonella (iNTS) disease, which represents one of the major causes of death and morbidity in sub-Saharan Africa, still partially underestimated....

  • Protocol
  • Open Access
15 Citations
4,782 Views
11 Pages

RIAM: A Universal Accessible Protocol for the Isolation of High Purity DNA from Various Soils and Other Humic Substances

  • Alexander G. Pinaev,
  • Arina A. Kichko,
  • Tatiana S. Aksenova,
  • Vera I. Safronova,
  • Elena V. Kozhenkova and
  • Evgeny E. Andronov

16 December 2022

A single universal open protocol RIAM (named after Research Institute for Agricultural Microbiology) for the isolation of high purity DNA from different types of soils and other substrates (high and low in humic, clay content, organic fertilizer, etc...

  • Protocol
  • Open Access
3 Citations
2,557 Views
10 Pages

The Design and Implementation of the ECOVIR Project: A Primary Health Care Surveillance System to Strengthen Co-Detection of Respiratory Viruses in Normandy

  • Hortense Petat,
  • Matthieu Schuers,
  • Sandrine Corbet,
  • Xavier Humbert,
  • François Le Bas,
  • Christophe Marguet,
  • Lucille Pellerin,
  • Andry Rabiaza,
  • Astrid Vabret and
  • Meriadeg Ar Gouilh

13 December 2022

Acute respiratory infections (ARIs) need to be better understood and treated, as they are critical to public health, especially during crises such as the SARS-CoV2 pandemic. These are the most abundant infections in the general population and are see...

  • Article
  • Open Access
6 Citations
3,268 Views
9 Pages

Relationship between Posterior Permanent Dentition Pattern and Radiographic Changes of the Mandibular Condyle

  • Mahsa Esfehani,
  • Marayam Tofangchiha,
  • Neda Poorsayyah,
  • Haniyeh Rahimi,
  • Sarvin Kholafazadeh,
  • Sina Radfar,
  • Francesco Pagnoni,
  • Rodolfo Reda and
  • Luca Testarelli

4 December 2022

This study assessed the relationship between posterior permanent dentition and radiographic changes of the mandibular condyle. This descriptive, cross-sectional study was conducted on 300 panoramic radiographs of patients over 40 years of age (188 fe...

  • Protocol
  • Open Access
5 Citations
5,670 Views
26 Pages

30 November 2022

Transcriptome-wide analysis of RNA-binding partners is commonly achieved using UV crosslinking and immunoprecipitation (CLIP). Individual-nucleotide-resolution CLIP (iCLIP)enables identification of the specific position of the protein–RNA inter...

  • Protocol
  • Open Access
9 Citations
4,070 Views
9 Pages

Procalcitonin Evaluation of Antibiotic Use in COVID-19 Hospitalised Patients (PEACH): Protocol for a Retrospective Observational Study

  • Joanne Euden,
  • Philip Pallmann,
  • Detelina Grozeva,
  • Mahableshwar Albur,
  • Stuart E. Bond,
  • Lucy Brookes-Howell,
  • Paul Dark,
  • Thomas Hellyer,
  • Susan Hopkins and
  • Jonathan A. T. Sandoe
  • + 15 authors

28 November 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel virus responsible for the coronavirus disease 2019 (COVID-19) pandemic. Although COVID-19 is a viral illness, many patients admitted to hospital are prescribed antibiotics, based...

  • Protocol
  • Open Access
1 Citations
3,657 Views
14 Pages

Physical Movement Habit Formation in Sedentary Office Workers: Protocol Paper

  • Kailas Jenkins,
  • Jena Buchan,
  • Ryan E. Rhodes and
  • Kyra Hamilton

25 November 2022

Engaging in physical movement has a number of mental and physical health benefits, and yet 45% of Australia’s population do not meet the recommended guidelines for physical activity. The current study aims to develop an online habit-based inter...

  • Communication
  • Open Access
7 Citations
3,991 Views
10 Pages

A High-Throughput Fluorescence Polarization-Based Assay for the SH2 Domain of STAT4

  • Angela Berg,
  • Martin Gräber,
  • Sebastian Schmutzler,
  • Ralf Hoffmann and
  • Thorsten Berg

23 November 2022

The signal transducer and activation of transcription (STAT) proteins are a family of Src homology 2 (SH2) domain-containing transcription factors. The family member STAT4 is a mediator of IL-12 signalling and has been implicated in the pathogenesis...

  • Article
  • Open Access
5 Citations
3,633 Views
14 Pages

Determination of UV Filters in Waste Sludge Using QuEChERS Method Followed by In-Port Derivatization Coupled with GC–MS/MS

  • Cemile Yücel,
  • Ilgi Karapinar,
  • Serenay Ceren Tüzün,
  • Hasan Ertaş and
  • Fatma Nil Ertaş

23 November 2022

UV filters (UVFs) are widely used in personal care and in industrial products for protection against photodegradation. In recent years, their potential toxicological and environmental effects have received growing attention. Due to their excessive us...

  • Article
  • Open Access
10 Citations
2,939 Views
14 Pages

Predictive Analysis of Maxillary Canine Impaction through Sella Turcica Bridging, Ponticulus Posticus Calcification, and Lateral Incisor Anomalies: A Retrospective Observational Study

  • Rosanna Guarnieri,
  • Francesca Germanò,
  • Federica Altieri,
  • Michele Cassetta,
  • Camilla Grenga,
  • Gabriella Padalino,
  • Roberto Di Giorgio and
  • Ersilia Barbato

21 November 2022

Maxillary canine impaction is an increasing dental anomaly and is often related to other dento-skeletal anomalies. The aim of this work is to support the clinician in evaluating the relationship between a displaced maxillary canine and clinical (the...

  • Article
  • Open Access
9 Citations
2,972 Views
10 Pages

1 November 2022

The drug combination of rosuvastatin (ROS) and ezetimibe (EZE) is used to treat hypercholesterolemia. In this work, a simultaneous electrochemical examination of ROS and EZE was conducted for the first time. The electrochemical determination of ROS a...

  • Article
  • Open Access
6 Citations
6,555 Views
14 Pages

Optimized Lambda Exonuclease Digestion or Purification Using Streptavidin-Coated Beads: Which One Is Best for Successful DNA Aptamer Selection?

  • Lisa Lucie Le Dortz,
  • Clotilde Rouxel,
  • Quentin Leroy,
  • Noah Brosseau,
  • Henri-Jean Boulouis,
  • Nadia Haddad,
  • Anne-Claire Lagrée and
  • Pierre Lucien Deshuillers

29 October 2022

The high failure rate of the in vitro aptamer selection process by SELEX (Systematic Evolution of Ligands by EXponential enrichment) limits the production of these innovative oligonucleotides and, consequently, limits their potential applications. Th...

  • Protocol
  • Open Access
7 Citations
4,595 Views
7 Pages

28 October 2022

Introduction: Exposure to natural hazards such as fire, drought, floods, and earthquakes can have negative impacts on physical and mental health and wellbeing. The social and structural factors contributing to individual and community vulnerability a...

  • Study Protocol
  • Open Access
5 Citations
4,105 Views
12 Pages

26 October 2022

Introduction: Dental caries in the adult population that require preventive and therapeutic treatment are generally neglected in rural communities. The determination of the effectiveness of the application of 38% silver diamine fluoride (SDF) in arre...

  • Opinion
  • Open Access
2,535 Views
3 Pages

25 October 2022

Sabbaticals should be a main feature of every academic career. This article provides some personal insights from experience along with an analysis of the benefits to the home and host institutions. Furthermore, the paper includes extensive and easy-t...

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Methods Protoc. - ISSN 2409-9279