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Progress in Analytical Techniques and Chemical Analysis in Molecular and Cellular Neuroscience

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Neurobiology".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 2561

Special Issue Editors

Dr. Amir Hatamie

E-Mail Website
Guest Editor
Department of Chemistry and Molecular Biology, University of Gothenburg, 41296 Gothenburg, Sweden
Interests: nano/microscale sensors; nanopore electrode; single-cell analysis; intracellular analysis; sensors for cell- and brain-tissue engineering; bioelectrochemistry; 2D materials for sensing applications; wearable (bio)sensors; flexible electrochemical sensors; (bio)sensors in plant science
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Andrew G. Ewing

E-Mail Website
Guest Editor
Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden
Interests: electrochemical; separations; imaging with optical and mass spectrometry methods aimed at understanding the chemistry of single nerve cells, single vesicles; neural communication in cell networks and in the Drosophila nervous system; molecular messengers in cell differentiation; analytical chemistry methods; micro- and nanoscale; neurochemistry; chemical engineering; biochemistry and molecular biology; basic medicine

Special Issue Information

Dear Colleagues, 

we are pleased to welcome you to submit a paper to this special Issue on “Progress in Analytical Techniques and Chemical Analysis in Molecular and Cellular Neuroscience”, which are evolving research subjects. The scope of the issue in the field of single cell and organelle and brain samples analysis is wide, including but not limited to the following areas:

  1. Recent development in intra-extracellular electrochemical analysis.
  2. Recent development in studying and analysis of neurochemicals (e.g., neuromodulators, neurotransmitters, etc.)
  3. Recent advances in mass spectrometry techniques for the analysis of single cell, organelle, and brain samples.
  4. Lab-on-a-chip and microfluidic platforms coupled with other analytical methods.
  5. Recent progress in super-resolution microscopy techniques.
  6. New fluorescence microscopy reagents, labels, and probes for live cell imaging.
  7. Analysis and monitoring of targets involved in neurodegenerative disease and neural disorders, such as reactive oxygen/nitrogen species (ROS/RNS), proteins, metabolites, ions, etc.
  8. Recent advances in sampling and sample preparation techniques.

Dr. Amir Hatamie
Prof. Dr. Andrew G. Ewing
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • neurodegenerative disease
  • neurotransmitters
  • single-cell analysis and electroanalysis
  • fluorescent probes
  • cell-based biosensors
  • fluorescence microscopy
  • cellular electrochemistry
  • single-cell amperometry
  • mass spectrometry
  • exocytosis
  • chemical messengers
  • super-resolution microscopy

Published Papers (2 papers)

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Research

14 pages, 4601 KiB  
Article
Characterization of Humanized Mouse Model of Organophosphate Poisoning and Detection of Countermeasures via MALDI-MSI
by Caitlin M. Tressler, Benjamin Wadsworth, Samantha Carriero, Natalie Dillman, Rachel Crawford, Tae-Hun Hahm, Kristine Glunde and C. Linn Cadieux
Int. J. Mol. Sci. 2024, 25(11), 5624; https://doi.org/10.3390/ijms25115624 - 22 May 2024
Viewed by 753
Abstract
Organophosphoate (OP) chemicals are known to inhibit the enzyme acetylcholinesterase (AChE). Studying OP poisoning is difficult because common small animal research models have serum carboxylesterase, which contributes to animals’ resistance to OP poisoning. Historically, guinea pigs have been used for this research; however, [...] Read more.
Organophosphoate (OP) chemicals are known to inhibit the enzyme acetylcholinesterase (AChE). Studying OP poisoning is difficult because common small animal research models have serum carboxylesterase, which contributes to animals’ resistance to OP poisoning. Historically, guinea pigs have been used for this research; however, a novel genetically modified mouse strain (KIKO) was developed with nonfunctional serum carboxylase (Es1 KO) and an altered acetylcholinesterase (AChE) gene, which expresses the amino acid sequence of the human form of the same protein (AChE KI). KIKO mice were injected with 1xLD50 of an OP nerve agent or vehicle control with or without atropine. After one to three minutes, animals were injected with 35 mg/kg of the currently fielded Reactivator countermeasure for OP poisoning. Postmortem brains were imaged on a Bruker RapifleX ToF/ToF instrument. Data confirmed the presence of increased acetylcholine in OP-exposed animals, regardless of treatment or atropine status. More interestingly, we detected a small amount of Reactivator within the brain of both exposed and unexposed animals; it is currently debated if reactivators can cross the blood–brain barrier. Further, we were able to simultaneously image acetylcholine, the primary affected neurotransmitter, as well as determine the location of both Reactivator and acetylcholine in the brain. This study, which utilized sensitive MALDI-MSI methods, characterized KIKO mice as a functional model for OP countermeasure development. Full article
(This article belongs to the Special Issue Progress in Analytical Techniques and Chemical Analysis in Molecular and Cellular Neuroscience)
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12 pages, 3040 KiB  
Article
Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes
by Young Ju Kim, Mina Jang, Jongtae Roh, Yoon Jeong Lee, Hee Jung Moon, Jimin Byun, Jihyun Wi, Sung-Kyun Ko and Jinsung Tae
Int. J. Mol. Sci. 2023, 24(20), 15073; https://doi.org/10.3390/ijms242015073 - 11 Oct 2023
Viewed by 1130
Abstract
Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been [...] Read more.
Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been of great interest due to their ON/OFF fluorescence depending on the spirolactam ring’s opening/closure. By introducing the N-alkyl-hydroxamic acid instead of the alkyl amines in the spirolactam of rhodamine, we were able to tune the pH range where the ring opening and closing of the spirolactam occurs. This six-membered cyclic hydroxamate spirolactam ring of rhodamine B proved to be highly fluorescent in acidic pH environments. In addition, we could monitor pH changes of lysosomes in live cells and zebrafish. Full article
(This article belongs to the Special Issue Progress in Analytical Techniques and Chemical Analysis in Molecular and Cellular Neuroscience)
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