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New Advances in Medical Microbiology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 15 December 2024 | Viewed by 1281

Special Issue Editor


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Guest Editor
Laboratory of Clinical Microbiology, Virology and Bioemergencies, Luigi Sacco Hospital, ASST Fatebenefratelli Sacco, Milan, Italy
Interests: clinical microbiology; clinical virology; SARS-CoV-2; HPV; sexually transmitted infections; respiratory viruses

Special Issue Information

Dear Colleagues,

The field of medical microbiology has witnessed significant advancements, integrating both traditional and cutting-edge techniques. Real-Time Polymerase Chain Reaction (PCR), Digital Droplet PCR, and Next-Generation Sequencing represent some of the significant improvements introduced for research and diagnostics of infections responsible for human diseases.

Novel strategies for surveillance and antimicrobial and antiviral stewardship programs could represent advancements in medical microbiology as well. In addition, it is very likely that artificial intelligence systems, including large language models (LLMs), will play a role in the diagnosis and management of infectious diseases.

Antimicrobial resistance (AMR) and multidrug resistant organisms (MDROs), emerging and re-emerging pathogens, and infection in transplant recipients and other immunocompromised individuals all represent areas that might benefit from these new approaches, potentially allowing more effective and reliable outcomes.

This Special Issue welcomes original scientific research articles, reviews, reports, and communications related to these topics.

Dr. Alberto Rizzo
Guest Editor

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Keywords

  • clinical microbiology
  • clinical virology
  • SARS-CoV-2
  • HPV
  • sexually transmitted infections
  • respiratory viruses
  • emerging pathogens
  • opportunistic infections

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Published Papers (1 paper)

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Research

19 pages, 1676 KiB  
Article
Diarrheagenic Escherichia coli in Stool Specimens Collected from Patients Attending Primary Healthcare Facilities in Ethiopia: Whole-Genome Sequencing-Based Molecular Characterization
by Deneke Wolde, Tadesse Eguale, Girmay Medhin, Aklilu Feleke Haile, Haile Alemayehu, Adane Mihret, Mateja Pirs, Katja Strašek Smrdel, Jana Avberšek, Darja Kušar, Tjaša Cerar Kišek, Tea Janko, Andrej Steyer and Marjanca Starčič Erjavec
Int. J. Mol. Sci. 2024, 25(19), 10251; https://doi.org/10.3390/ijms251910251 - 24 Sep 2024
Viewed by 818
Abstract
The diarrheagenic Escherichia coli (DEC) is the major cause of diarrheal diseases in Africa, including Ethiopia. However, the genetic diversity of E. coli pathotypes found in Ethiopia has not been studied well. This study aimed to characterize potential DEC belonging to enteropathogenic (EPEC), [...] Read more.
The diarrheagenic Escherichia coli (DEC) is the major cause of diarrheal diseases in Africa, including Ethiopia. However, the genetic diversity of E. coli pathotypes found in Ethiopia has not been studied well. This study aimed to characterize potential DEC belonging to enteropathogenic (EPEC), Shiga toxin-producing (STEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) E. coli pathotypes from stool specimens of patients attending primary healthcare units (n = 260) in Addis Ababa and Hossana using whole-genome sequencing. Real-time PCR assays were used to identify DEC isolates belonging to EPEC, STEC, EAEC, ETEC, and EIEC pathotypes, which were then subjected to whole-genome sequencing on the Illumina platform. Twenty-four whole-genome nucleotide sequences of DEC strains with good enough quality were analyzed for virulence-associated genes (VAGs), antibiotic resistance genes (ARGs), phylogenetic groups, serogroups, and sequence types. The majority (62.5%) of DEC isolates belonged to the phylogenetic group B1. The identified DEC isolates belonged to 21 different serogroups and 17 different sequence types. All tested DEC isolates carried multiple VAGs and ARGs. The findings highlight the high diversity in the population structure of the studied DEC isolates, which is important for designing targeted interventions to reduce the diarrheal burden in Ethiopia. Full article
(This article belongs to the Special Issue New Advances in Medical Microbiology)
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