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Advances in In Vitro Culture Systems for Plant Propagation and...
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Advances in In Vitro Culture Systems for Plant Propagation and Conservation

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 921

Special Issue Editor

Prof. Dr. Jeffery Adelberg

E-Mail Website
Guest Editor
Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA
Interests: plant propagation systems; in vitro culture; micropropagation; in vitro rooting; controlled environment agriculture
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The journal Plants is announcing a Special Issue focussing on ‘Advances in In Vitro Culture Systems for Plant Propagation and Conservation’. Agriculture and forest crops are in ever-increasing demand to supply vast amounts of natural products while using fewer resources. Our plant collections are the single most important asset to meet this demand. Rapid increases in core collections without spreading disease or creating genetic drift is the most important application of micropropagation technologies. Restoring damaged environments with appropriate genetics, or populating controlled environments for sustainable harvests, delivers downstream benefits to society at large. We invite you to share your research with our audience as it pertains to this Special Issue.

Prof. Dr. Jeffery Adelberg
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • germplasm
  • repository
  • cryopreservation
  • clonal

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

10 pages, 4326 KiB  
Article
Modified Media for Repeated In Vitro Cutting Cycles of Cannabis sativa Without the Use of Cytokinin
by Molly McKay, James E. Faust, Matthew Taylor and Jeffrey Adelberg
Plants 2025, 14(7), 1138; https://doi.org/10.3390/plants14071138 - 6 Apr 2025
Viewed by 147
Abstract
In vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with Cannabis sativa (‘Cherry1’, ‘BaOx’, ‘T1’, ‘Peach’) grown in vessels of three different [...] Read more.
In vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with Cannabis sativa (‘Cherry1’, ‘BaOx’, ‘T1’, ‘Peach’) grown in vessels of three different physical states—stationary agar (A); stationary Oasis® infused with liquid (OILs); and agitated Oasis® infused with liquid (OILa). Vessels were pre-selected as control or supplemented; where supplement vessels received 15 mL DKW liquid media each cycle harvest. The number of shoot tips harvested; shoot length; and dry shoot mass from repeated cutting cycles was recorded. In a single harvest; ‘BaOx’ and ‘Cherry 1’ produced one shoot per plant from the original 15 planted on all treatments. ‘Peach’ and ‘T1’ produced less shoots on average; but the most in OIL treatments. All shoots harvested were longer in OIL compared to A; regardless of genotype. Over multiple cycles; ‘Peach’ and ‘T1’ were unable to reliably produce shoots on a repeated schedule and were, therefore, eliminated from the experiment. By cycle 3; maximum number of plants were produced; regardless of supplementation (‘Cherry 1’; 30; ‘BaOx’; 22). Shoot length was above 10 mm (planting standard) for both genotypes until after the third cycle (10 weeks) where number and quality decreased (nodes and internodes easily discerned). By the end of the experiment; the only shoots that remained productive for over 16 weeks and multiple repeated harvest cycles were those in OIL treatments with supplements. Full article
(This article belongs to the Special Issue Advances in In Vitro Culture Systems for Plant Propagation and Conservation)
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11 pages, 3995 KiB  
Article
The Effects of Blue Light and Supplemental Far-Red on an In Vitro Multiple Harvest System for the Production of Cannabis sativa
by Molly McKay, James E. Faust, Matthew Taylor and Jeffrey Adelberg
Plants 2025, 14(6), 966; https://doi.org/10.3390/plants14060966 - 19 Mar 2025
Viewed by 518
Abstract
Blue and supplemental far-red light were observed to affect in vitro shoot growth with Cannabis sativa (‘BaOx’ and ‘Cherry 1’) in RV750 vessels. A modified “hedging” and fed-batch system for multiple harvests using Oasis® foam and 120 mL DKW medium was used. [...] Read more.
Blue and supplemental far-red light were observed to affect in vitro shoot growth with Cannabis sativa (‘BaOx’ and ‘Cherry 1’) in RV750 vessels. A modified “hedging” and fed-batch system for multiple harvests using Oasis® foam and 120 mL DKW medium was used. Fifteen nodal and/or apical tips were planted and placed into PAR light treatments providing various red to blue ratios (polychromatic white 9:1 RB and dichromatic 2–15: 1 RB, with and without 5% far-red light). Treatments had similar light intensities (190–240 µmol · m−2 · s−1 PPFD) for a 16 h photoperiod. Shoot tips were harvested in vitro on five successive two-week cycles, with 15 mL of DKW media supplemented to each vessel following harvest. Shoot numbers, length, and fresh and dry mass were recorded at each cycle harvest. Five randomly selected shoot tips per vessel were rooted ex vitro on greenhouse mist bench for 16 days. Over multiple cycles, 5% far-red increased shoot numbers and length in both genotypes tested, regardless of polychromatic or dichromatic source. Shoots harvested per vessel increased from 15 to 28 in three cycles (6 weeks), but increased from 15 to 18 without far-red treatment. Shoot length in far-red-treated plants increased from 19 to 25 mm during cycles 1–3. Plants without far-red treatment were approximately 15 mm during the first three cycles. By cycle 5, both far-red- and non-far-red-treated plants decreased to 10 mm. Dry mass was greatest in cycle 1 for both genotypes (‘Cherry 1’ was 6 mg and ‘BaOx’ was 7 mg) under the highest amount of blue light, but 2 mg under the lowest amount of blue light. Dry mass decreased by 50% in cycle 3, to 4 mg, where it remained for the duration of the experiment. Sixty eight percent of shoots rooted ex vitro on the mist bench, regardless of any prior in vitro treatment. Full article
(This article belongs to the Special Issue Advances in In Vitro Culture Systems for Plant Propagation and Conservation)
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