Plant Propagation

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 1804

Special Issue Editors


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Guest Editor
Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA
Interests: Plant propagation systems; in vitro culture; micropropagation; in vitro rooting; controlled environment agriculture

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Guest Editor
Department of Horticulture, Division of Applied Life Science, Graduate School, Gyeongsang National University (GNU), Jinju 52828, Republic of Korea
Interests: floriculture; transplants (micropropagated and plug); silicon in horticulture; plant factory; protected horticulture; hydroponics
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Special Issue Information

Dear Colleagues,

Plant propagation is the process of growing new plants from old plants by various methods, such as collecting seeds, cuttings, or other parts of plants. Research on plant propagation systems addresses difficult issues across commodity disciplines. Advances in biotechnology can create new opportunities for the rational use and regeneration of valuable plant material through the adoption of techniques such as in vitro culture and conservation. In vitro culture of plants is also a direct tool to obtain more productive and dynamic plants (including plants without diseases and pests), which is conducive to the production of more efficient and high-yielding plants in the field, especially through micro-propagation. This Special Issue will focus on new methods of in vitro culture and propagation, major innovations, advances, or improvements in existing technology, or elucidating new in vitro plant cultivation applications. These approaches can positively impact plant development, disease resistance, mineral nutrition, propagation, and germplasm conservation.

Prof. Dr. Jeffery Adelberg
Prof. Dr. Byoung Ryong Jeong
Guest Editors

Manuscript Submission Information

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Keywords

  • propagation
  • in vitro plant cultivation
  • biotechnology
  • plant tissue culture
  • micropropagation
  • rooting
  • conservation
  • in vitro propagation
  • tissue culture
  • transplants
  • plant factory
  • controlled environment agriculture

Published Papers (1 paper)

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Research

20 pages, 10652 KiB  
Article
Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots
by Yiming Zhang, Jiahui Zhang, Junliang Yin, Yiqing Liu and Xiaodong Cai
Plants 2023, 12(22), 3789; https://doi.org/10.3390/plants12223789 - 07 Nov 2023
Cited by 1 | Viewed by 885
Abstract
The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro [...] Read more.
The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro plant regeneration from the stem nodes of the Jerusalem artichoke via direct organogenesis is presented. In the shoot induction stage, the stem segments produced more shoots with vigorous growth on MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA). The concentrations of 6-BA and gibberellic acid (GA3) were both optimized at 0.5 mg/L for shoot multiplication, and the combination of 0.05 mg/L indole-3-butyric acid (IBA) and 0.05 mg/L 1-naphthylacetic acid (NAA) was the most responsive for root induction, yielding the largest number of roots. The regenerated plantlets were successfully hardened at a 96% survival rate and vigorously grew in the field. The genetic stability of the regenerated plants was confirmed by flow cytometry and simple sequence repeat (SSR) analysis. However, 17.3% of shoots on the optimum shoot induction medium had withered leaves and excessive callus (atypical shoots), which greatly reduced the induction efficiency. Enzyme activity in the typical and atypical shoots was compared. The atypical shoots had significantly higher levels of endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA), as well as increased activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), whereas the content of 6-BA, zeatin (ZT), and GA3 was significantly reduced. The activity of the three enzymes was positively correlated with the content of IAA and ABA, while being negatively correlated with that of 6-BA, ZT, and GA3. The results suggest that the poor growth of the atypical shoots might be closely related to the significant accumulation of endogenous IAA and ABA, thus significantly increasing antioxidant enzyme activity. Full article
(This article belongs to the Special Issue Plant Propagation)
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