Genomic Breeding of Green Crops

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 14084

Special Issue Editors


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Guest Editor
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Interests: seed storage; seed quality; grain nutrition; plant architecture; plant genetics; gene diversity; rice genomic breeding

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Guest Editor
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Interests: QTL/gene clones; genomic breeding; plant genetics; plant architecture
Special Issues, Collections and Topics in MDPI journals
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Interests: wheat; stripe rust; leaf rust; powdery mildew; QTL mapping; gene cloning; marker assistant selection; wheat breeding

Special Issue Information

Dear Colleagues,

We are facing a major challenge—growing global population and dwindling resources of land, water, and costly inputs for food production. To address this challenge, plant scientists proposed the concept of “green crops” that highlights promoting resource-saving and environment-friendly crop production, while still achieving yield increase and quality improvement. This requires a solid understanding of the genetic architecture, genes, and network(s) underlying the “green traits” to ensure the improvement of yield and grain quality, with less input of pesticide, fertilizer, and irrigation water. Recently, advancements in genome sequencing and functional genomics have led to efficient and versatile tools for gene discovery and green crop improvement. In particular, genomic selection strategies with high-throughput genotyping technologies are being established and applied in several breeding programs, which will accelerate the precise improvement of target traits in crop varieties. This Special Issue will focus on the genetic improvement of cereal crops such as rice, maize and wheat by genomic breeding and a gene-specific selection system. We mostly welcome related topics (original research papers, perspectives, and reviews) covering the genes/QTLs for yield, grain quality, grain nutrition, resource use efficiency, resistance to biotic/abiotic stresses, genome editing, genetic transformation, gene chip and molecular marker-aided selection, as well as speed breeding approaches in staple food crops.

Prof. Dr. Sibin Yu
Dr. Fazhan Qiu
Dr. Caixia Lan
Guest Editors

Manuscript Submission Information

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Keywords

  • food security
  • environment health
  • biotic stress
  • abiotic stress
  • resource-use efficiency
  • QTL/gene mapping and cloning
  • marker-assisted selection
  • gene editing
  • genomic breeding

Published Papers (6 papers)

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Research

16 pages, 2332 KiB  
Article
Pleiotropic Effect of the compactum Gene and Its Combined Effects with Other Loci for Spike and Grain-Related Traits in Wheat
by Mingxing Wen, Jiaxuan Su, Chengzhi Jiao, Xu Zhang, Tao Xu, Tong Wang, Xiaoxue Liu, Zongkuan Wang, Li Sun, Chunxia Yuan, Haiyan Wang, Xiue Wang and Jin Xiao
Plants 2022, 11(14), 1837; https://doi.org/10.3390/plants11141837 - 13 Jul 2022
Cited by 8 | Viewed by 2942
Abstract
Club wheat (Triticum aestivum ssp. compactum) with a distinctly compact spike morphology was conditioned by the dominant compactum (C) locus on chromosome 2D and resulted in a redistribution of spike yield components. The disclosure of the genetic basis of [...] Read more.
Club wheat (Triticum aestivum ssp. compactum) with a distinctly compact spike morphology was conditioned by the dominant compactum (C) locus on chromosome 2D and resulted in a redistribution of spike yield components. The disclosure of the genetic basis of club wheat was a prerequisite for the development of widely adapted, agronomically competitive club wheat cultivars. In this study, we used a recombinant inbred line population derived from a cross between club wheat Hiller and modern cultivar Yangmai 158 to construct a genetic linkage map and identify quantitative trait loci associated with 15 morphological traits. The club allele acted in a semi-dominant manner and the C gene was mapped to 370.12–406.29 Mb physical region on the long arm of 2D. Apart from compact spikes, C exhibited a pleiotropic effect on ten other agronomic traits, including plant height, three spike-related traits and six grain-related traits. The compact spike phenotype was correlated with decreased grain size and weight, but with an increase in floret fertility and grain number. These pleiotropic effects make club wheat have compatible spike weight with a normal spike from common wheat. The genetic effects of various gene combinations of C with four yield-related genes, including Ppd-D1, Vrn-D3, Rht-B1b and Rht8, were evaluated. C had no epistatic interaction with any of these genes, indicating that their combinations would have an additive effect on other agronomically important traits. Our research provided a theoretical foundation for the potentially effective deployment of C gene into modern breeding varieties in combination with other favorable alleles. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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24 pages, 5617 KiB  
Article
Insights into the Regulation of Rice Seed Storability by Seed Tissue-Specific Transcriptomic and Metabolic Profiling
by Fangzhou Liu, Nannan Li, Yuye Yu, Wei Chen, Sibin Yu and Hanzi He
Plants 2022, 11(12), 1570; https://doi.org/10.3390/plants11121570 - 14 Jun 2022
Cited by 6 | Viewed by 1844
Abstract
Non-dormant seeds are continuously aging and deteriorating during storage, leading to declining seed vigor, which is a challenge for the rice seed industry. Improving the storability of seeds is of great significance to ensure the quality of rice and national food security. Through [...] Read more.
Non-dormant seeds are continuously aging and deteriorating during storage, leading to declining seed vigor, which is a challenge for the rice seed industry. Improving the storability of seeds is of great significance to ensure the quality of rice and national food security. Through a set of chromosome segment substitution lines population constructed using japonica rice NIP as donor parent and indica rice ZS97 as recurrent parent, we performed seed storability QTL analysis and selected four non-storable NILs to further investigate the storability regulatory mechanisms underlying it. The seeds were divided into four tissues, which were the embryo, endosperm, aleurone layer, and hull, and tissue-specific transcriptome and metabolome analyses were performed on them. By exploring the common differentially expressed genes and differentially accumulated metabolites, as well as the KEGG pathway of the four non-storable NILs, we revealed that the phenylpropanoid biosynthesis pathway and diterpenoid biosynthesis pathway played a central role in regulating seed storability. Integrated analysis pinpointed 12 candidate genes that may take part in seed storability. The comprehensive analysis disclosed the divergent and synergistic effect of different seed tissues in the regulation of rice storability. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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10 pages, 1673 KiB  
Article
Verifying the Breeding Value of A Rare Haplotype of Chalk7, GS3, and Chalk5 to Improve Grain Appearance Quality in Rice
by Dianwen Wang, Jilin Wang, Wenqiang Sun, Xianjin Qiu, Zhiyang Yuan and Sibin Yu
Plants 2022, 11(11), 1470; https://doi.org/10.3390/plants11111470 - 30 May 2022
Cited by 4 | Viewed by 1644
Abstract
Grain quality is a key determinant of commercial value in rice. Efficiently improving grain quality, without compromising grain yield, is a challenge in rice breeding programs. Here we report on the identification and application of a grain quality gene, Chalk7, which causes a [...] Read more.
Grain quality is a key determinant of commercial value in rice. Efficiently improving grain quality, without compromising grain yield, is a challenge in rice breeding programs. Here we report on the identification and application of a grain quality gene, Chalk7, which causes a slender shape and decreases grain chalkiness in rice. Three allele-specific markers for Chalk7, and two other grain genes (GS3 and Chalk5) were developed, and used to stack the desirable alleles at these loci. The effects of individual or combined alleles at the loci were evaluated using a set of near-isogenic lines, each containing one to three favorable alleles in a common background of an elite variety. We found that the favorable allele combination of the three loci, which rarely occurs in natural rice germplasm, greatly reduces chalky grains without negatively impacting on grain yield. The data for newly developed allele-specific markers and pre-breeding lines will facilitate the improvement of grain appearance quality in rice. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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14 pages, 977 KiB  
Article
Genetic Dissection of Rice Ratooning Ability Using an Introgression Line Population and Substitution Mapping of a Pleiotropic Quantitative Trait Locus qRA5
by Hui Hu, Ruoyu Gao, Liping He, Famao Liang, Zhixin Li, Junying Xu, Longwei Yang, Chongrong Wang, Zhangyong Liu, Jianlong Xu and Xianjin Qiu
Plants 2022, 11(9), 1134; https://doi.org/10.3390/plants11091134 - 22 Apr 2022
Cited by 3 | Viewed by 1548
Abstract
Ratooning ability is a key factor that influences ratoon rice yield, in the area where light and temperature are not enough for second season rice. In the present study, an introgression line population derived from Minghui 63 as the recipient parent and 02428 [...] Read more.
Ratooning ability is a key factor that influences ratoon rice yield, in the area where light and temperature are not enough for second season rice. In the present study, an introgression line population derived from Minghui 63 as the recipient parent and 02428 as the donor parent was developed, and a high-density bin map containing 4568 bins was constructed. Nine ratooning-ability-related traits were measured, including maximum tiller number, panicle number, and grain yield per plant in the first season and ratoon season, as well as three secondary traits, maximum tiller number ratio, panicle number ratio, and grain yield ratio. A total of 22 main-effect QTLs were identified and explained for 3.26–18.63% of the phenotypic variations in the introgression line population. Three genomic regions, including 14.12–14.65 Mb on chromosome 5, 4.64–5.76 Mb on chromosome 8, and 10.64–15.52 Mb on chromosome 11, were identified to simultaneously control different ratooning-ability-related traits. Among them, qRA5 in the region of 14.12–14.65 Mb on chromosome 5 was validated for its pleiotropic effects on maximum tiller number and panicle number in the first season, as well as its maximum tiller number ratio, panicle number ratio, and grain yield ratio. Moreover, qRA5 was independent of genetic background and delimited into a 311.16 kb region by a substitution mapping approach. These results will help us better understand the genetic basis of rice ratooning ability and provide a valuable gene resource for breeding high-yield ratoon rice varieties. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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22 pages, 8869 KiB  
Article
Genome-Wide Analysis of AAT Genes and Their Expression Profiling during Fiber Development in Cotton
by Dongjie Yang, Yuanyuan Liu, Hailiang Cheng, Qiaolian Wang, Limin Lv, Youping Zhang, Dongyun Zuo and Guoli Song
Plants 2021, 10(11), 2461; https://doi.org/10.3390/plants10112461 - 15 Nov 2021
Cited by 4 | Viewed by 2086
Abstract
Amino acid transporters (AATs) are a kind of membrane proteins that mediate the transport of amino acids across cell membranes in higher plants. The AAT proteins are involved in regulating plant cell growth and various developmental processes. However, the biological function [...] Read more.
Amino acid transporters (AATs) are a kind of membrane proteins that mediate the transport of amino acids across cell membranes in higher plants. The AAT proteins are involved in regulating plant cell growth and various developmental processes. However, the biological function of this gene family in cotton fiber development is not clear. In this study, 190, 190, 101, and 94 full-length AAT genes were identified from Gossypiumhirsutum, G. barbadense, G. arboreum, and G. raimondii. A total of 575 AAT genes from the four cotton species were divided into two subfamilies and 12 clades based on phylogenetic analysis. The AAT genes in the four cotton species were distributed on all the chromosomes. All GhAAT genes contain multiple exons, and each GhAAT protein has multiple conserved motifs. Transcriptional profiling and RT qPCR analysis showed that four GhATT genes tend to express specifically at the fiber initiation stage. Eight genes tend to express specifically at the fiber elongation and maturity stage, and four genes tend to express specifically at the fiber initiation and elongation stages. Our results provide a solid basis for further elucidating the biological function of AAT genes related to cotton fiber development and offer valuable genetic resources for crop improvement in the future. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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14 pages, 1753 KiB  
Article
Fine Mapping of a Major Pleiotropic QTL Associated with Sesamin and Sesamolin Variation in Sesame (Sesamum indicum L.)
by Fangtao Xu, Rong Zhou, Senouwa Segla Koffi Dossou, Shengnan Song and Linhai Wang
Plants 2021, 10(7), 1343; https://doi.org/10.3390/plants10071343 - 30 Jun 2021
Cited by 13 | Viewed by 2622
Abstract
Deciphering the genetic basis of quantitative agronomic traits is a prerequisite for their improvement. Herein, we identified loci governing the main sesame lignans, sesamin and sesamolin variation in a recombinant inbred lines (RILs, F8) population under two environments. The content of the two [...] Read more.
Deciphering the genetic basis of quantitative agronomic traits is a prerequisite for their improvement. Herein, we identified loci governing the main sesame lignans, sesamin and sesamolin variation in a recombinant inbred lines (RILs, F8) population under two environments. The content of the two lignans in the seeds was investigated by HPLC. The sesamin and sesamolin contents ranged from 0.33 to 7.52 mg/g and 0.36 to 2.70 mg/g, respectively. In total, we revealed 26 QTLs on a linkage map comprising 424 SSR markers, including 16 and 10 loci associated with sesamin and sesamolin variation, respectively. Among them, qSmin_11.1 and qSmol_11.1 detected in both the two environments explained 67.69% and 46.05% of the phenotypic variation of sesamin and sesamolin, respectively. Notably, qSmin11-1 and qSmol11-1 were located in the same interval of 127–127.21 cM on LG11 between markers ZMM1776 and ZM918 and acted as a pleiotropic locus. Furthermore, two potential candidate genes (SIN_1005755 and SIN_1005756) at the same locus were identified based on comparative transcriptome analysis. Our results suggest the existence of a single gene of large effect that controls expression, both of sesamin and sesamolin, and provide genetic information for further investigation of the regulation of lignan biosynthesis in sesame. Full article
(This article belongs to the Special Issue Genomic Breeding of Green Crops)
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