Plants

31 pages, 5106 KiB

Open AccessReview

Genus Echium L.: Phytochemical Characterization and Bioactivity Evaluation for Drug Discovery

by Parvaneh Sheydaei, Maria Emília Amaral and Ana Paula Duarte

Plants 2025, 14(16), 2548; https://doi.org/10.3390/plants14162548 - 15 Aug 2025

Echium L. is a genus of flowering plants from the Boraginaceae family that includes several species traditionally used in herbal medicine. Echium spp. have been applied for treating wounds, urinary tract infections, inflammation, respiratory ailments, cardiovascular disorders, and microbial infections. The roots and [...] Read more.

Echium L. is a genus of flowering plants from the Boraginaceae family that includes several species traditionally used in herbal medicine. Echium spp. have been applied for treating wounds, urinary tract infections, inflammation, respiratory ailments, cardiovascular disorders, and microbial infections. The roots and flowers are most frequently used, typically prepared as decoctions or infusions. Phytochemical studies have identified diverse bioactive compounds, including phenolics, naphthoquinones, shikonins, fatty acids, sterols, terpenoids, amino acids, and toxic pyrrolizidine alkaloids. Reported pharmacological effects include antioxidant, antimicrobial, and cytotoxic activities, primarily attributed to polyphenolic and terpenoid content. However, the presence of toxic alkaloids also raises concerns regarding safety. This review provides a comprehensive overview of the ethnomedicinal uses, phytochemical components, and pharmacological activities of Echium species. The bioactivities observed in genus Echium L. substantiate the necessity for preclinical and clinical investigations to thoroughly elucidate and validate the therapeutic potential of this genus and emphasize its relevance in the development of novel therapeutic agents. Full article

(This article belongs to the Special Issue Phytochemical Investigations for Drug Discovery, Development and Biotechnological Approaches for Health and Agriculture)

► Show Figures

Figure 1

26 pages, 4573 KiB

Open AccessArticle

Characterization of the Mitochondrial Genome of Hippophae rhamnoides subsp. sinensis Rousi Based on High-Throughput Sequencing and Elucidation of Its Evolutionary Mechanisms

by Mengjiao Lin, Na Hu, Jing Sun and Wu Zhou

Plants 2025, 14(16), 2547; https://doi.org/10.3390/plants14162547 - 15 Aug 2025

Abstract

Hippophae rhamnoides ssp. sinensis Rousi a species of significant ecological and economic value that is native to the Qinghai–Tibet Plateau and arid/semi-arid regions. Investigating the mitochondrial genome can elucidate stress adaptation mechanisms, population genetic structure, and hybrid evolutionary history, offering molecular insights for [...] Read more.

Hippophae rhamnoides ssp. sinensis Rousi a species of significant ecological and economic value that is native to the Qinghai–Tibet Plateau and arid/semi-arid regions. Investigating the mitochondrial genome can elucidate stress adaptation mechanisms, population genetic structure, and hybrid evolutionary history, offering molecular insights for ecological restoration and species conservation. However, the genetic information and evolutionary mechanisms of its mitochondrial genome remain poorly understood. This study aimed to assemble the complete mitochondrial genome of H. rhamnoides L. ssp. sinensis using Illumina sequencing, uncovering its structural features, evolutionary pressures, and environmental adaptability and addressing the research gap regarding mitochondrial genomes within the Hippophae genus. The study assembled a 454,444 bp circular mitochondrial genome of H. rhamnoides ssp. sinensis, with a GC content of 44.86%. A total of 73 genes and 3 pseudogenes were annotated, with the notable absence of the rps2 gene, which is present in related species. The genome exhibits significant codon usage bias, particularly with high-frequency use of the alanine codon GCU and the isoleucine codon AUU. Additionally, 449 repetitive sequences, potentially driving genome recombination, were identified. Our evolutionary pressure analysis revealed that most genes are under purifying selection, while genes such as atp4 and nad4 exhibit positive selection. A nucleotide diversity analysis revealed that the sdh4 gene exhibits the highest variation, whereas rrn5 is the most conserved. Meanwhile, phylogenetic analysis showed that H. rhamnoides ssp. sinensis from China is most closely related to Hippophae tibetana, with extensive homologous sequences (49.72% of the chloroplast genome) being identified between the chloroplast and mitochondrial genomes, indicating active inter-organellar gene transfer. Furthermore, 539 RNA editing sites, primarily involving hydrophilic-to-hydrophobic amino acid conversions, were predicted, potentially regulating mitochondrial protein function. Our findings establish a foundation for genetic improvement and research on adaptive evolutionary mechanisms in the Hippophae genus, offering a novel case study for plant mitochondrial genome evolution theory. Full article

(This article belongs to the Special Issue Crop Genome Sequencing and Analysis)

20 pages, 2272 KiB

Open AccessArticle

Interspecific Associations of Dominant Tree Species at Different Structural Levels and Community Stability in the Habitat of Endangered Plant Hopea hainanensis Merr. & Chun

by Shaocui He, Donghai Li, Xiaobo Yang, Dongling Qi, Naiyan Shang, Caiqun Liang, Rentong Liu, Chunyan Du, Hao Ding and Binglin Ye

Plants 2025, 14(16), 2546; https://doi.org/10.3390/plants14162546 - 15 Aug 2025

Abstract

The endangered plant Hopea hainanensis serves as both an indicator and keystone species in tropical rainforests, and its survival status is influenced by the interspecific relationships among coexisting tree species within the community. To explore these relationships, species resource utilization patterns, and community [...] Read more.

The endangered plant Hopea hainanensis serves as both an indicator and keystone species in tropical rainforests, and its survival status is influenced by the interspecific relationships among coexisting tree species within the community. To explore these relationships, species resource utilization patterns, and community succession dynamics within the endangered plant community, this study utilized survey data from the Hopea hainanensis community in the Bawangling and Jianfengling branches of the National Park of Hainan Tropical Rainforest. Various analytical methods were employed, including the Variance Ratio (VR) method, test statistic (W), χ² test, Spearman’s rank correlation, and M. Godron’s stability analysis, to examine the interspecific associations among dominant tree species at different structural levels in the two regions and their effects on community stability. The results indicate that: (1) Hopea hainanensis is the dominant species in the medium tree layer in both study areas, while it functions as an associated species in other structural layers. (2) In communities where Hopea hainanensis is present in both Bawangling and Jianfengling, the dominant tree species across various structural layers generally show a non-significant positive association. (3) The results of the χ² test and Spearman’s rank correlation test reveal that the interspecific associations across different structural layers of the Hopea hainanensis communities in both regions are predominantly non-significant. This suggests weak interspecific relationships and a high degree of species independence. The communities at different structural levels in both Bawangling and Jianfengling are in an unstable state, with ongoing dynamic adjustments to their internal tree species composition and structure. In terms of stability, the community stability across structural levels in these two regions follows the order: middle shrub layer > middle arbor layer > small arbor layer > large shrub layer. This study reveals the interspecific relationships, community succession status, and stability of dominant tree species at different structural levels in slope barrier communities across regions. These findings provide a theoretical basis for developing scientifically sound and reasonable protection strategies for slope barrier populations, as well as for the restoration and sustainable development of tropical rainforest vegetation. Full article

(This article belongs to the Section Plant Ecology)

19 pages, 3177 KiB

Open AccessArticle

Phosphorus-Driven Stem-Biased Allocation: NPK Synergy Optimizes Growth and Physiology in Dalbergia odorifera T. C. Chen Seedlings

by Mengwen Zhang, Chuanteng Huang, Ling Lin, Lin Chen, Xiaoli Yang, Xiaona Dong, Jiaming Song and Feifei Chen

Plants 2025, 14(16), 2545; https://doi.org/10.3390/plants14162545 - 15 Aug 2025

Abstract

Valued for furniture, crafts, and medicine, Dalbergia odorifera T. C. Chen confronts critically depleted wild populations and slow cultivation growth, necessitating precision nutrient formulation to overcome physiological constraints. Using a ‘3414’ regression design with four levels of N, P, and K, this study [...] Read more.

Valued for furniture, crafts, and medicine, Dalbergia odorifera T. C. Chen confronts critically depleted wild populations and slow cultivation growth, necessitating precision nutrient formulation to overcome physiological constraints. Using a ‘3414’ regression design with four levels of N, P, and K, this study identified phosphorus (P) as the most influential nutrient in regulating growth (P > N > K). Maximal growth enhancement occurred under T7 (N2P3K2), with height and basal diameter increments increasing by 239% and 128% versus controls (p < 0.05). Both traits exhibited progressive gains with rising P but unimodal responses to N and K, initially increasing then declining. T7 boosted total biomass by 50% (p < 0.05) with stem-biased partitioning (stem > root > leaf; 52%, 26%, 22%). Photosynthetic capacity increased significantly under T7 (p < 0.05), driven by P-mediated chlorophyll gains (Chla + 70%; Chlb + 75%) and an 82% higher net photosynthetic rate. Metabolic shifts revealed peak soluble sugar in T7 (+139%) and soluble protein in T9 (+226%) (p < 0.05), associated primarily with P and K availability, respectively. Correlation networks revealed significant associations among structural growth, photosynthesis, and metabolism. Principal component analysis established T7 as optimal, defining a “medium-N, high-P medium-K” precision fertilization protocol. These findings elucidate a phosphorus-centered regulatory mechanism governing growth in D. odorifera, providing a scientific foundation for efficient cultivation. Full article

(This article belongs to the Section Plant Nutrition)

► Show Figures

Figure 1

30 pages, 1292 KiB

Open AccessReview

Advances in UAV Remote Sensing for Monitoring Crop Water and Nutrient Status: Modeling Methods, Influencing Factors, and Challenges

by Xiaofei Yang, Junying Chen, Xiaohan Lu, Hao Liu, Yanfu Liu, Xuqian Bai, Long Qian and Zhitao Zhang

Plants 2025, 14(16), 2544; https://doi.org/10.3390/plants14162544 - 15 Aug 2025

Abstract

With the advancement of precision agriculture, Unmanned Aerial Vehicle (UAV)-based remote sensing has been increasingly employed for monitoring crop water and nutrient status due to its high flexibility, fine spatial resolution, and rapid data acquisition capabilities. This review systematically examines recent research progress [...] Read more.

With the advancement of precision agriculture, Unmanned Aerial Vehicle (UAV)-based remote sensing has been increasingly employed for monitoring crop water and nutrient status due to its high flexibility, fine spatial resolution, and rapid data acquisition capabilities. This review systematically examines recent research progress and key technological pathways in UAV-based remote sensing for crop water and nutrient monitoring. It provides an in-depth analysis of UAV platforms, sensor configurations, and their suitability across diverse agricultural applications. The review also highlights critical data processing steps—including radiometric correction, image stitching, segmentation, and data fusion—and compares three major modeling approaches for parameter inversion: vegetation index-based, data-driven, and physically based methods. Representative application cases across various crops and spatiotemporal scales are summarized. Furthermore, the review explores factors affecting monitoring performance, such as crop growth stages, spatial resolution, illumination and meteorological conditions, and model generalization. Despite significant advancements, current limitations include insufficient sensor versatility, labor-intensive data processing chains, and limited model scalability. Finally, the review outlines future directions, including the integration of edge intelligence, hybrid physical–data modeling, and multi-source, three-dimensional collaborative sensing. This work aims to provide theoretical insights and technical support for advancing UAV-based remote sensing in precision agriculture. Full article

(This article belongs to the Special Issue Remote Sensing Application in Augmenting Water and Fertilizer Utilization for Sustainable Agriculture)

► Show Figures

Figure 1

25 pages, 6376 KiB

Open AccessArticle

RdDM-Associated Chromatin Remodelers in Soybean: Evolution and Stress-Induced Expression of CLASSY Genes

by Paula Machado de Araújo, Arthur Gruber, Liliane Santana OIiveira, Sara Sangi, Geovanna Vitória Olimpio, Felipe Cruz Paula and Clícia Grativol

Plants 2025, 14(16), 2543; https://doi.org/10.3390/plants14162543 - 15 Aug 2025

Abstract

RNA-directed DNA methylation (RdDM) is an epigenetic mechanism involved in several biological processes in plants, requiring complex machinery including the chromatin remodeling protein CLASSY (CLSY). The CLSY family regulates global and locus-specific DNA methylation and was initially identified in Arabidopsis thaliana. Despite [...] Read more.

RNA-directed DNA methylation (RdDM) is an epigenetic mechanism involved in several biological processes in plants, requiring complex machinery including the chromatin remodeling protein CLASSY (CLSY). The CLSY family regulates global and locus-specific DNA methylation and was initially identified in Arabidopsis thaliana. Despite reports in other plants, detailed knowledge about CLSY proteins in soybean is scarce. In this work, we used profile hidden Markov models (profile HMMs) specifically constructed for CLSY detection to identify new members in soybean and to analyze their phylogenetic relationships across bryophyte, basal angiosperm, basal eudicot, monocots, and eudicots. We identified two new candidates for CLSY1-2 and one for DRD1 in soybean and, for the first time, detected CLSY and DRD1 genes in Aquilegia coerulea. Phylogenetic analysis indicated two main CLSY groups: one similar to Arabidopsis CLSY1-2 and another to CLSY3-4. Gene duplication analysis demonstrated that whole-genome duplication/segmental duplication events contributed to CLSY family expansion in soybean. RT-qPCR analysis showed that CLSY and five other epigenetic regulator genes had stress-modulated expression during soybean germination under salt and osmotic stress, with variation among cultivars. Our findings enhance comprehension of the evolutionary dynamics of the CLSY family and furnish insights into their response to abiotic stress in soybean. Full article

(This article belongs to the Special Issue Molecular Regulation of Plant Stress Responses)

19 pages, 3953 KiB

Open AccessArticle

Greens in the Gaps: Diversity and the Ecological Potential of Urban Spontaneous Vegetation in Sidewalk Ecosystems

by Nadchawan Charoenlertthanakit, Angkhana Inta, Dia Panitnard Shannon, Boonchuang Boonsuk and Pimonrat Tiansawat

Plants 2025, 14(16), 2542; https://doi.org/10.3390/plants14162542 - 15 Aug 2025

Abstract

Green spaces are essential for urban environments, but urban expansion often results in fragmented patches and narrow pavements unsuitable for tree growth. Consequently, there is a pressing need for alternative vegetation in urban landscapes where tree planting is impractical. Urban spontaneous vegetation (USV)—plants [...] Read more.

Green spaces are essential for urban environments, but urban expansion often results in fragmented patches and narrow pavements unsuitable for tree growth. Consequently, there is a pressing need for alternative vegetation in urban landscapes where tree planting is impractical. Urban spontaneous vegetation (USV)—plants that establish naturally without cultivation—shows promise for urban landscaping, and yet has been underexplored in urban ecology. This study was the first systematic survey to examine the composition of USV in Chiang Mai, Thailand, across seven urban locations. The survey was conducted along 13 sidewalk routes (totaling 33.24 km), documenting all non-tree vascular plant species. A total of 63 USV species from 24 families were recorded, predominantly colonizing pavement gaps, cracks, and curbside cracks. The most diverse family was Poaceae, with 15 species. Among the 61 identified species, 32 species (52%) were non-native. Seven species were found in all surveyed locations, highlighting their adaptability to challenging urban conditions. Fifty USV species are medicinal plants. Many species exhibit characteristics that are ideal for sustainable landscaping, such as drought tolerance, low maintenance requirements, and ornamental value. This study highlights USV as a key component of green infrastructure and provides new insights for urban sustainable landscaping. Full article

(This article belongs to the Special Issue Flora and Vegetation in Cities: Basic and Applied Scientific Knowledge for Environmental Sustainability)

► Show Figures

Figure 1

23 pages, 8300 KiB

Open AccessArticle

Pan-Genome-Based Characterization of the PYL Transcription Factor Family in Populus

by Xiaoli Han, Chen Qiu, Zhongshuai Gai, Juntuan Zhai, Jia Song, Jianhao Sun and Zhijun Li

Plants 2025, 14(16), 2541; https://doi.org/10.3390/plants14162541 - 15 Aug 2025

Abstract

Abscisic acid (ABA) is a key phytohormone involved in regulating plant growth and responses to environmental stress. As receptors of ABA, pyrabactin resistance 1 (PYR)/PYR1-like (PYL) proteins play a central role in initiating ABA signal transduction. In this study, a total of 30 [...] Read more.

Abscisic acid (ABA) is a key phytohormone involved in regulating plant growth and responses to environmental stress. As receptors of ABA, pyrabactin resistance 1 (PYR)/PYR1-like (PYL) proteins play a central role in initiating ABA signal transduction. In this study, a total of 30 PopPYL genes were identified and classified into three sub-families (PYL I–III) in the pan-genome of 17 Populus species, through phylogenetic analysis. Among these subfamilies, the PYL I subfamily was the largest, comprising 21 members, whereas PYL III was the smallest, with only four members. To elucidate the evolutionary dynamics of these genes, we conducted synteny and Ka/Ks analyses. Results indicated that most PopPYL genes had undergone purifying selection (Ka/Ks < 1), while a few were subject to positive selection (Ka/Ks > 1). Promoter analysis revealed 258 cis-regulatory elements in the PYL genes of Populus euphratica (EUP) and Populus pruinosa (PRU), including 127 elements responsive to abiotic stress and 33 ABA-related elements. Furthermore, six structural variations (SVs) were detected in PYL_EUP genes and significantly influenced gene expression levels (p < 0.05). To further explore the functional roles of PYL genes, we analyzed tissue-specific expression profiles of 17 PYL_EUP genes under drought stress conditions. PYL6_EUP was predominantly expressed in roots, PYL17_EUP exhibited leaf-specific expression, and PYL1_EUP showed elevated expression in stems. These findings suggest that the drought response of PYL_EUP genes is tissue-specific. Overall, this study highlights the utility of pan-genomics in elucidating gene family evolution and suggests that PYL_EUP genes contribute to the regulation of drought stress responses in EUP, offering valuable genetic resources for functional characterization of PYL genes. Full article

(This article belongs to the Special Issue Physiological and Molecular Mechanisms of Plant Resistance to Abiotic Stress)

► Show Figures

Figure 1

18 pages, 1999 KiB

Open AccessArticle

Early Sowing Approach for Developing Climate Resilient Maize: Cold Stress Impact on Germination of Adapted Inbred Lines with High Nutritive Value

by Marija Kostadinović, Mirjana Milovanović, Ana Nikolić, Ksenija Marković, Jelena Vukadinović, Jelena Vančetović and Dragana Ignjatović Micić

Plants 2025, 14(16), 2540; https://doi.org/10.3390/plants14162540 - 15 Aug 2025

Abstract

In temperate regions, early sowing of high nutritive genotypes could support maize production sustainability by avoiding warming-related unfavorable environment conditions during flowering. Seven standard maize (SM) lines and their nine quality protein maize (QPM) counterparts were evaluated for cold tolerance during germination. Cold [...] Read more.

In temperate regions, early sowing of high nutritive genotypes could support maize production sustainability by avoiding warming-related unfavorable environment conditions during flowering. Seven standard maize (SM) lines and their nine quality protein maize (QPM) counterparts were evaluated for cold tolerance during germination. Cold stress (13°/6 °C) was applied for five days, after a 48 h imbibition period under optimal temperature (25°/22 °C). Germination, physiological parameters, and some primary and secondary metabolites in the seeds were analyzed. No significant differences (p > 0.05) were observed in cold tolerance between SM and QPM. Cold stress significantly reduced germination energy (SM-p < 0.05, QPM-p < 0.001) and physiological traits (p < 0.001), with shoot traits being most severely affected. The potentially high impact of gallic (p < 0.001), protocatechuic (p < 0.05), and p-coumaric (p < 0.001) acids on germination under stress and negative effect of lutein + zeaxhantin and β-cryptoxhantin (p < 0.05) on root length was revealed. Among all lines, L3QPM excelled under stress, with unchanged germination energy and the lowest fold change in vigor indices (0.35 for VI1, 0.45 for VI2). Also, β + γ-tocopherol and gallic and caffeic acids were significantly higher (p < 0.05) compared to its SM original. Lines L1QPM2, L3QPM, and L7QPM, combining improved nutritional quality with high cold tolerance, will be incorporated in further early sowing research and breeding programs. Full article

(This article belongs to the Section Plant Physiology and Metabolism)

► Show Figures

Figure 1

25 pages, 7157 KiB

Open AccessArticle

Climate Change Drives Northwestward Migration of Betula alnoides: A Multi-Scenario MaxEnt Modeling Approach

by Yangzhou Xiang, Qiong Yang, Suhang Li, Ying Liu, Yuan Li, Jun Ren, Jiaxin Yao, Xuqiang Luo, Yang Luo and Bin Yao

Plants 2025, 14(16), 2539; https://doi.org/10.3390/plants14162539 - 15 Aug 2025

Abstract

Climate change poses unprecedented challenges to forest ecosystems. Betula alnoides, a tree species with significant ecological and economic value in southern China, has been the subject of studies on its distribution pattern and response to climate change. However, research on the distribution [...] Read more.

Climate change poses unprecedented challenges to forest ecosystems. Betula alnoides, a tree species with significant ecological and economic value in southern China, has been the subject of studies on its distribution pattern and response to climate change. However, research on the distribution pattern of B. alnoides and its response to climate change remains relatively limited. In this study, we developed a MaxEnt model incorporating multiple environmental variables, including climate, topography, soil, vegetation, and human activities, to evaluate model performance, identify key factors influencing the distribution of B. alnoides, and project its potential distribution under various future climate scenarios. Species occurrence data and environmental layers were compiled for China, and model parameters were optimized using the ENMeval package. The results showed that the optimized model achieved an AUC value of 0.956, indicating extremely high predictive accuracy. The four key factors affecting the distribution of B. alnoides were standard deviation of temperature seasonality (Bio4), normalized difference vegetation index (NDVI), mean temperature of driest quarter (Bio9), and annual precipitation (Bio12). Among them, the cumulative contribution rate of climatic factors reached 68.9%, but the influence of NDVI was significantly higher than that of precipitation factors. The current suitable habitat of B. alnoides is mainly concentrated in the southwestern region, covering an area of 179.32 × 10⁴ km², which accounts for 18.68% of China’s land area. Under the SSP126 scenario, the suitable habitat area first decreases and then increases in the future, while under the SSP370 and SSP585 scenarios, the suitable habitat area continues to shrink, with significant losses in high-suitability areas. In addition, the centroid of the suitable habitat of B. alnoides shows an overall trend of shifting northwestward. This indicates that B. alnoides is highly sensitive to climate change and its distribution pattern will undergo significant changes in the future. In conclusion, the distribution pattern of B. alnoides shows a significant response to climate change, with particularly prominent losses in high-suitability areas in the future. Therefore, it is recommended to strengthen the protection of high-suitability areas in the southwestern region and consider B. alnoides as an alternative tree species for regions facing warming and drying trends to enhance its climate adaptability. Full article

(This article belongs to the Section Plant Modeling)

► Show Figures

Figure 1

15 pages, 3096 KiB

Open AccessArticle

Optimization of Swertiamarin and Isogentisin Extraction from Gentiana lutea L. Leaves by Response Surface Methodology

by Katarina Šavikin, Miloš S. Jovanović, Gordana Zdunić, Jelena Živković, Dušanka Kitić, Dubravka Bigović and Teodora Janković

Plants 2025, 14(16), 2538; https://doi.org/10.3390/plants14162538 - 15 Aug 2025

Abstract

Leaves of Gentiana lutea L., traditionally used for treating heart disorders, represent a sustainable and underutilized source of bitter secoiridoids and xanthones, also found in Gentianae radix—an official herbal drug derived from the same, protected species. As root harvesting leads to the [...] Read more.

Leaves of Gentiana lutea L., traditionally used for treating heart disorders, represent a sustainable and underutilized source of bitter secoiridoids and xanthones, also found in Gentianae radix—an official herbal drug derived from the same, protected species. As root harvesting leads to the destruction of the plant, using the more readily available leaves could help reduce the pressure on this endangered natural resource. This study aimed to optimize the ultrasound-assisted extraction of the secoiridoid swertiamarin and the xanthone isogentisin from G. lutea leaves using response surface methodology (RSM). Subsequently, the stability of the bioactive compounds (swertiamarin, gentiopicrin, mangiferin, isoorientin, isovitexin, and isogentisin) in the optimized extract was monitored over a 30-day period under different storage conditions. The influence of extraction time (5–65 min), ethanol concentration (10–90% v/v), liquid-to-solid ratio (10–50 mL/g), and temperature (20–80 °C) was analyzed at five levels according to a central composite design. The calculated optimal extraction conditions for the simultaneous maximization of swertiamarin and isogentisin yields were 50 min extraction time, 30% v/v ethanol concentration, 30 mL/g liquid-to-solid ratio, and 62.7 °C extraction temperature. Under these conditions, the experimentally obtained yields were 3.75 mg/g dry weight for swertiamarin and 1.57 mg/g dry weight for isogentisin, closely matching the RSM model predictions. The stability study revealed that low-temperature storage preserved major bioactive compounds, whereas mangiferin stability was compromised by elevated temperature and light exposure. The established models support the production of standardized G. lutea leaf extracts and may facilitate the efficient separation and purification of their bioactive compounds, thereby contributing to the further valorization of this valuable plant material. Full article

(This article belongs to the Special Issue Efficacy, Safety and Phytochemistry of Medicinal Plants)

► Show Figures

Figure 1

13 pages, 1062 KiB

Open AccessArticle

Can the DSE Fungus Exserohilum rostratum Mitigate the Effect of Salinity on the Grass Chloris gayana?

by Natalia Elizabeth Tobar Gomez, Marcos Ameijeiras, Hernan E. Benitez, Federico N. Spagnoletti, Viviana M. Chiocchio and Raúl S. Lavado

Plants 2025, 14(16), 2537; https://doi.org/10.3390/plants14162537 - 15 Aug 2025

Abstract

Dark septate endophytes (DSEs) are commonly found in saline environments, such as the Flooding Pampas (Argentina), where the forage grass Chloris gayana has been introduced. This study evaluated the effect of salinity on the DSE fungus Exserohilum rostratum, isolated from C. gayana [...] Read more.

Dark septate endophytes (DSEs) are commonly found in saline environments, such as the Flooding Pampas (Argentina), where the forage grass Chloris gayana has been introduced. This study evaluated the effect of salinity on the DSE fungus Exserohilum rostratum, isolated from C. gayana, and its contribution to the grass’s salinity tolerance. Two greenhouse experiments were conducted under three salinity levels (0, 40, and 80 meq Na·L⁻¹), with and without fungal inoculation. Fungal growth, root colonization, functional traits, plant biomass, chemical composition, and salinity tolerance indices were assessed. The fungus tolerated salinity and colonized roots, showing qualitative evidence of enzyme production and phosphate solubilization. In both experiments, shoot and root biomass decreased with increasing salinity. Inoculation significantly enhanced shoot biomass only under non-saline conditions in the first experiment, whereas in the second experiment no inoculation effect was observed on shoots. For roots, no effect of inoculation occurred in the first experiment, but a positive interaction between salinity and inoculation was recorded in the second experiment, where moderate salinity increased root biomass in inoculated plants. The K/Na and Ca/Na ratios decreased under salinity regardless of inoculation, indicating limited influence on ionic balance. These results suggest that although E. rostratum tolerates salinity and expresses functional traits, its ability to enhance plant performance under stress is context-dependent and restricted to specific conditions. Full article

(This article belongs to the Special Issue Mycorrhizal and Endophytic Fungi as a Tool for Sustainable Environments)

► Show Figures

Figure 1

15 pages, 3581 KiB

Open AccessArticle

Phenotypic Variation of 933 Broomcorn Millet (Panicum miliaceum L.) Germplasm Resources

by Yuyao Kong, Xia Zhang, Haoyang Li, Yirong Qiu, Hanghang Hou, Xiaoling Zhang, Baili Feng and Qinghua Yang

Plants 2025, 14(16), 2536; https://doi.org/10.3390/plants14162536 - 15 Aug 2025

Abstract

Studying comprehensive performance is fundamental for the effective utilisation of broomcorn millet (Panicum miliaceum L.) germplasm resources and breeding of new varieties. However, compared with other major crops, research on broomcorn millet germplasm resources is limited, and the trait variations of broomcorn [...] Read more.

Studying comprehensive performance is fundamental for the effective utilisation of broomcorn millet (Panicum miliaceum L.) germplasm resources and breeding of new varieties. However, compared with other major crops, research on broomcorn millet germplasm resources is limited, and the trait variations of broomcorn millet are unclear. In this study, three qualitative and seven quantitative traits of 933 broomcorn millet core collections were analysed to provide the basis for improving utilisation of broomcorn millet germplasm resources. The seed colour was a strong phenotypic trait and had eight variants. The 933 resources exhibited three panicle types: lateral (74.5%), scattered (18.4%), and compact (7.1%). They exhibited two inflorescence colours: green (54.7%) and purple (45.3%). Pearson’s correlation analysis revealed that 1000-seed weight significantly correlated with plant height, length of panicle, and number of main stem segments. The period of duration positively correlated with 1000-seed weight but negatively correlated with the number of uniserial panicles. Cluster analysis based on the quantitative traits indicated that all resources were divided into three groups, and each group had its respective characteristics. The analysis of core germplasm resources of broomcorn millet in this study provided a basis to explore excellent genes and for breeding of excellent varieties. Full article

(This article belongs to the Section Plant Genetic Resources)

► Show Figures

Figure 1

14 pages, 4807 KiB

Open AccessArticle

Identification of Candidate Genes Related to the Husk Papillae in Foxtail Millet (Setaria italica (L.) P. Beauv)

by Meixia Tan, Yang Yang, Zhe Chen, Xiangyuan Gong, Fangfang Ma, Ming Duan, Lidong Wang and Yuanhuai Han

Plants 2025, 14(16), 2535; https://doi.org/10.3390/plants14162535 - 14 Aug 2025

Abstract

Efficient and fast water uptake by seeds, facilitated by optimal soil moisture, plays a critical role in timely germination and early seedling vigor for foxtail millet production in arid and semi-arid regions. The husk, as a unique structure through which the seed contacts [...] Read more.

Efficient and fast water uptake by seeds, facilitated by optimal soil moisture, plays a critical role in timely germination and early seedling vigor for foxtail millet production in arid and semi-arid regions. The husk, as a unique structure through which the seed contacts the soil, plays an important role in water uptake and germination. Many foxtail millet germplasm accessions have papillae on the epidermis of their husks, yet the role of this trait in water uptake and germination, as well as the genetic basis and regulatory mechanism related to this trait, remain unknown. In this study, we demonstrated that the water uptake by the seeds from accessions with papillae was significantly higher than that of accessions without papillae two hours and four hours after sowing during a 10 h experiment, resulting in faster germination. Analysis of segregating ratios from two F2 populations derived from crossing between accessions with and without papillae indicated that husk papilla density was of monogenic dominance. Bulked Segregant Analysis Sequencing (BSA-Seq) showed that candidate regions on chromosome 5 were significantly associated with husk papilla density. The mapped region overlapped by the two BSA populations for papilla density included 72 genes. In combination with the expression profiles of these genes, five candidate genes were identified, encoding aquaporins, fructose transporter, and glycoside hydrolase. This study elucidated the role of husk papillae in enhancing water uptake and germination in foxtail millet, provided genetic insights into the trait, and laid the foundation for further study on the mechanism of husk papilla differentiation. Full article

(This article belongs to the Section Plant Molecular Biology)

► Show Figures

Figure 1

21 pages, 2752 KiB

Open AccessArticle

Endophytic Bacterial and Fungal Communities of Spruce Picea jezoensis in the Russian Far East

by Nikolay N. Nityagovsky, Alexey A. Ananev, Andrey R. Suprun, Alina A. Dneprovskaya, Konstantin V. Kiselev and Olga A. Aleynova

Plants 2025, 14(16), 2534; https://doi.org/10.3390/plants14162534 - 14 Aug 2025

Abstract

A wide range of microorganisms, including endophytes, frequently interact with forest trees. The role of endophytes in industrial conifers has not been fully investigated. The Yezo spruce Picea jezoensis is widely used for logging in Russia and Japan. In this work, the endophytic [...] Read more.

A wide range of microorganisms, including endophytes, frequently interact with forest trees. The role of endophytes in industrial conifers has not been fully investigated. The Yezo spruce Picea jezoensis is widely used for logging in Russia and Japan. In this work, the endophytic communities of bacteria and fungi in healthy needles, branches, and fresh wood of P. jezoensis from Primorsky Territory were analyzed using metagenomic analysis. The results indicate that the diversity of endophytic communities in P. jezoensis is predominantly influenced by the specific tree parts (for both bacteria and fungi) and by different tree specimens (for fungi). The most abundant bacterial classes were Alphaproteobacteria, Gammaproteobacteria and Actinobacteria. Functional analysis of KEGG orthologs (KOs) in endophytic bacterial community using PICRUSt2 and the PLaBAse PGPT ontology revealed that 59.5% of the 8653 KOs were associated with plant growth-promoting traits (PGPTs), mainly, colonization, stress protection, bio-fertilization, bio-remediation, vitamin production, and competition. Metagenomic analysis identified a high abundance of the genera Pseudomonas and Methylobacterium-Methylorubrum in P. jezoensis, which are known for their potential growth-promoting activity in other coniferous species. The dominant fungal classes in P. jezoensis were Dothideomycetes, Sordariomycetes, and Eurotiomycetes. Notably, the genus Penicillium showed a pronounced increase in relative abundance within the fresh wood and needles of Yezo spruce, while Aspergillus displayed elevated abundance specifically in the fresh wood. It is known that some of these fungi exhibit antagonistic activity against phytopathogenic fungi. Thus, our study describes endophytic communities of the Yezo spruce and provides a basis for the production of biologicals with potential applications in forestry and agriculture. Full article

(This article belongs to the Special Issue Plant-Microbiome Interactions)

► Show Figures

Figure 1

28 pages, 14601 KiB

Open AccessArticle

Balancing Accuracy and Computational Efficiency: A Faster R-CNN with Foreground-Background Segmentation-Based Spatial Attention Mechanism for Wild Plant Recognition

by Zexuan Cui, Zhibo Chen and Xiaohui Cui

Plants 2025, 14(16), 2533; https://doi.org/10.3390/plants14162533 - 14 Aug 2025

Abstract

Computer vision recognition technology, due to its non-invasive and convenient nature, can effectively avoid damage to fragile wild plants during recognition. However, balancing model complexity, recognition accuracy, and data processing difficulty on resource-constrained hardware is a critical issue that needs to be addressed. [...] Read more.

Computer vision recognition technology, due to its non-invasive and convenient nature, can effectively avoid damage to fragile wild plants during recognition. However, balancing model complexity, recognition accuracy, and data processing difficulty on resource-constrained hardware is a critical issue that needs to be addressed. To tackle these challenges, we propose an improved lightweight Faster R-CNN architecture named ULS-FRCN. This architecture includes three key improvements: a Light Bottleneck module based on depthwise separable convolution to reduce model complexity; a Split SAM lightweight spatial attention mechanism to improve recognition accuracy without increasing model complexity; and unsharp masking preprocessing to enhance model performance while reducing data processing difficulty and training costs. We validated the effectiveness of ULS-FRCN using five representative wild plants from the PlantCLEF 2015 dataset. Ablation experiments and multi-dataset generalization tests show that ULS-FRCN significantly outperforms the baseline model in terms of mAP, mean F1 score, and mean recall, with improvements of 12.77%, 0.01, and 9.07%, respectively. Compared to the original Faster R-CNN, our lightweight design and attention mechanism reduce training parameters, improve inference speed, and enhance computational efficiency. This approach is suitable for deployment on resource-constrained forestry devices, enabling efficient plant identification and management without the need for high-performance servers. Full article

(This article belongs to the Special Issue The Future of Artificial Intelligence and Sensor Systems in Agriculture)

► Show Figures

Figure 1

18 pages, 10675 KiB

Open AccessArticle

Evolutionary Insights and Flowering Regulation of SPLs in Coconut Palm

by Runan Chen, Yalan Feng, Jin Zhou, Ying Wang, Fengyi Zhang, Shazia Rehman, Zhuang Yang, Zifen Lao, Hang Xu, Yong Xiao, Jie Luo and Wei Xia

Plants 2025, 14(16), 2532; https://doi.org/10.3390/plants14162532 - 14 Aug 2025

Abstract

Squamosa Promoter-Binding Protein Like (SPL) is a critical transcription factor that plays a significant role in regulating plant growth and development. Mining the coconut SPL family offers valuable insights into the regulation of important agronomic traits, including the length of the [...] Read more.

Squamosa Promoter-Binding Protein Like (SPL) is a critical transcription factor that plays a significant role in regulating plant growth and development. Mining the coconut SPL family offers valuable insights into the regulation of important agronomic traits, including the length of the juvenile phase. In this study, 25 CnSPLs were identified and were classified into eight subfamilies. Analysis of gene structure and conserved protein motifs indicated a high conservation of CnSPLs within the same subfamilies; however, variations in protein structure and gene length were observed across different subfamilies. Gene expansion analysis indicated that most gene members within subfamilies originated from duplications of the same genomic segment, and transposable element insertion contributed to the divergence of gene sequences within these subfamilies. Characterization of the miR156 target sequence in SPL transcripts revealed that subfamilies IV to VIII contained these sequences, while subfamilies I to III did not. In both coconut and 14 other plant species, some SPLs lost their miR156-binding loci due to gene structure variations. The gene expression profiles revealed significant divergence between miR156-targeted and non-targeted CnSPLs; the former exhibited low expression levels in the endosperm, while the latter showed comparable expression across all tissues. Notably, CnSPL15A demonstrated steadily increasing expression levels in leaves throughout successive leaf primordia and significantly promoted flowering when overexpressed in Arabidopsis. Transient expression assays and 5′ RACE confirmed that CnSPLs are targeted by miR156. This study establishes a foundation for investigating the evolutionary characteristics of CnSPLs and provides a theoretical framework for analyzing the functions of key CnSPLs involved in the coconut flowering control pathway. Full article

(This article belongs to the Special Issue Advancements in Palmaceae Research: Genomic Insights, Trait Analysis, and Breeding Innovations)

► Show Figures

Figure 1

27 pages, 3699 KiB

Open AccessArticle

by Linlin Cheng, Huichuan Jiang, Guishui Xie, Jikun Wang, Wentao Peng, Lijun Zhou, Wanting Liu, Dingquan Wu and Feng An

Plants 2025, 14(16), 2531; https://doi.org/10.3390/plants14162531 - 14 Aug 2025

Abstract

The purpose of this study was to establish the relationship between the chilling resistance of rubber trees and the bark-bleeding characteristics caused by chilling stress, considering physiological indicators in rubber tree bark, cell wall chemical components, fiber morphologies, and tensile properties. This offered [...] Read more.

The purpose of this study was to establish the relationship between the chilling resistance of rubber trees and the bark-bleeding characteristics caused by chilling stress, considering physiological indicators in rubber tree bark, cell wall chemical components, fiber morphologies, and tensile properties. This offered a unique perspective for examining the underlying mechanisms of latex bleeding and chilling stress in Hevea brasiliensis. One-year-old seedlings and two-year-old twig segments in five- and twenty-one-year-old rubber trees (5YB and 21YB) were used to compare the age-mediation differences in their various parameters. Meanwhile, the LT₅₀ values were calculated with Logistic regression analysis of relative electrical conductivity (REC) data under gradient low temperatures. Subsequently, changes in corresponding parameters of 1-year-old seedling stem bark at different ages were determined, and the bark-bleeding characteristics of seedlings and twig segments were analyzed under artificially simulated chilling stress, respectively. A correlation analysis between semi-lethal temperature (LT₅₀) values, relative water content (RWC) values, bark-bleeding characteristics, cell-wall chemical component contents, fiber dimensions, and tensile property parameters was implemented to estimate interrelationships among them. The LT₅₀ values ranged from −2.0387 °C to −0.8695 °C. The results showed that the chilling resistance order of rubber trees at different ages was as follows: 21YB (2-year-old twig bark from 21-year-old rubber trees) > 5YB (2-year-old twig bark from 5-year-old rubber trees) > SLB (semi-lignification bark in 1-year-old seedlings) > GB (green bark in 1-year-old seedlings). The chilling resistance of seedlings and twig segments in rubber trees was highly positively (p < 0.001) related to fiber morphologies. Chilling-induced bark-bleeding characteristics were significantly correlated (p < 0.001) with fiber morphologies, bark tensile properties, and cell-wall components. The analysis data in this study contribute towards building a comprehensive understanding of the mechanisms of chilling-induced bark bleeding needed not only in rubber tree cultivation but also in sustainable rubber production. Full article

(This article belongs to the Special Issue Systems-Level Understanding of Plant Adaptation to Abiotic Stress: Physiological and Biochemical Perspectives)

► Show Figures

Figure 1

16 pages, 3487 KiB

Open AccessArticle

Functional Analysis of the Autophagy-Related Gene OsATG4b in Rice Grain Chalkiness Regulation

by Zhi Hu, Xiang Han, Yumeng Yuan, Ruishan Xing, Hongchun Liu, Chenming Li, Hongli Shen, Yifan Guo, Shengyuan Sun, Yihao Yang, Min Guo and Changjie Yan

Plants 2025, 14(16), 2530; https://doi.org/10.3390/plants14162530 - 14 Aug 2025

Abstract

Grain chalkiness is an undesirable trait that significantly compromises rice quality, attracting considerable attention from both consumers and breeders. In this study, we characterized the role of the autophagy-related gene OsATG4b in rice grain development. OsATG4b was predominantly expressed in the endosperm. Compared [...] Read more.

Grain chalkiness is an undesirable trait that significantly compromises rice quality, attracting considerable attention from both consumers and breeders. In this study, we characterized the role of the autophagy-related gene OsATG4b in rice grain development. OsATG4b was predominantly expressed in the endosperm. Compared with wild-type plants, OsATG4b-overexpressing lines exhibited significantly reduced grain chalkiness, whereas OsATG4b knockout mutants displayed a marked increase in chalkiness. Importantly, OsATG4b had no significant effect on other major agronomic traits. Ultrastructure analysis of the endosperm and evaluation of seed storage components revealed that the chalky endosperm in OsATG4b Knockout mutants contained loosely packed starch granules, aberrant protein bodies, and reduced levels of seed storage proteins. Furthermore, gene expression analysis indicated that OsATG4b regulates the expression of genes involved in storage protein biosynthesis. Together, these findings demonstrate that OsATG4b plays a critical regulatory role in determining grain chalkiness in rice. Full article

(This article belongs to the Section Plant Molecular Biology)

► Show Figures

Figure 1

14 pages, 2685 KiB

Open AccessArticle

Intermediate Anthropogenic Disturbances Boost Taxonomic and Phylogenetic Diversities but Reduce Functional Diversity in Subtropical Forests of Eastern China

by Libin Liu, Xiaoyin Guan, Yunquan Wang, Jianhua Chen, Julian Liu, Shuisheng Yu, Zihong Zheng and Mingjian Yu

Plants 2025, 14(16), 2529; https://doi.org/10.3390/plants14162529 - 14 Aug 2025

Abstract

Anthropogenic disturbances significantly impact plant biodiversity in subtropical forests. While prior research has primarily concentrated on taxonomic diversity, other dimensions of biodiversity, such as phylogenetic and functional diversities, remain insufficiently explored. This study simultaneously investigated these three facets of plant diversity in subtropical [...] Read more.

Anthropogenic disturbances significantly impact plant biodiversity in subtropical forests. While prior research has primarily concentrated on taxonomic diversity, other dimensions of biodiversity, such as phylogenetic and functional diversities, remain insufficiently explored. This study simultaneously investigated these three facets of plant diversity in subtropical forests with two distinct disturbance histories in eastern China, aiming to elucidate the effects of intermediate anthropogenic disturbances on biodiversity. Disturbed deciduous broadleaf forests exhibited markedly lower Pielou evenness index values compared to their conserved counterparts (p < 0.05). Disturbed evergreen broadleaf forests demonstrated significantly higher species richness, Shannon–Wiener index scores, and phylogenetic diversity relative to those found in conserved forests (p < 0.05). Furthermore, both disturbed deciduous broadleaf and mixed evergreen–deciduous broadleaf forests displayed significantly reduced functional richness and quadratic entropy coefficient values when compared with their conserved equivalents (p < 0.05). Forest type exerted a significant influence on all three dimensions of biodiversity (p < 0.05). In conclusion, intermediate anthropogenic disturbances have the potential to enhance both plant taxonomic and phylogenetic diversities while concurrently diminishing functional diversity within the subtropical forests of eastern China. The mechanisms driving responses in plant diversity to intermediate anthropogenic disturbances vary according to forest types. Full article

(This article belongs to the Section Plant Systematics, Taxonomy, Nomenclature and Classification)

► Show Figures

Figure 1

12 pages, 1737 KiB

Open AccessArticle

Phenotypic Identification and Fine-Mapping of the Rice Narrow-Leaf Mutant nal25

by Kaizhen Xie, Fuan Niu, Peng Hu, Can Cheng, Huangwei Chu, Jihua Zhou, Bin Sun, Yuting Dai, Liming Cao and Anpeng Zhang

Plants 2025, 14(16), 2528; https://doi.org/10.3390/plants14162528 - 14 Aug 2025

Abstract

Leaf morphology significantly impacts rice (Oryza sativa L.) plant architecture and yield. Here, we identified and characterized a novel narrow-leaf mutant, nal25, derived from indica rice cultivar ‘Huazhan’ using EMS mutagenesis. Phenotypic analyses revealed that nal25 exhibited significantly narrower leaves, reduced [...] Read more.

Leaf morphology significantly impacts rice (Oryza sativa L.) plant architecture and yield. Here, we identified and characterized a novel narrow-leaf mutant, nal25, derived from indica rice cultivar ‘Huazhan’ using EMS mutagenesis. Phenotypic analyses revealed that nal25 exhibited significantly narrower leaves, reduced plant height, increased tiller number, and notably decreased grain size, seed setting rate, and thousand-grain weight compared to the wild type. Genetic analyses demonstrated that the narrow-leaf phenotype is controlled by a single recessive nuclear gene. Through precise localization analysis, the NAL25 gene was located within a region of approximately 103 kb on the long arm of rice chromosome 7. The sequencing results showed that the mutant nal25 had a T to C mutation at position 173 of the heat-shock protein gene LOC_Os07g09450 encoding the DnaJ domain in this interval, resulting in a change in amino acid 58 from leucine to proline. The qRT-PCR results showed that the expression level of NAL25 gene decreased in the mutant. The nal25 mutant obtained in this study exhibits stable mutant phenotypes, including dwarfism and excessive tillering, traits typically unfavorable for rice production. Nevertheless, it serves as valuable genetic material for forward genetics approaches to identify yield-related genes regulating leaf morphology and culm height. Thus, research on the nal25 mutant advances the development of rice varieties with ideal plant architecture, thereby stabilizing yield increases and safeguarding global food security. Full article

(This article belongs to the Section Crop Physiology and Crop Production)

► Show Figures

Figure 1

14 pages, 3633 KiB

Open AccessArticle

Insecticide Resistance Evolution Negatively Affects the Fitness of Aphis gossypii Glover During Selection on Cotton Plants Under Laboratory Conditions

by Hina Gul, Ali Güncan, Arzlan Abbas, Zeeshan Ullah, Xie Yuqing, Farman Ullah, Nicolas Desneux and Xiaoxia Liu

Plants 2025, 14(16), 2527; https://doi.org/10.3390/plants14162527 - 14 Aug 2025

Abstract

The cotton aphid, Aphis gossypii Glover, is among the most economically significant sap-sucking insect pests, inflicting substantial economic losses worldwide. Insecticides such as thiamethoxam, bifenthrin, and flonicamid are commonly used to manage this pest, despite the inherent risk of developing resistance. In this [...] Read more.

The cotton aphid, Aphis gossypii Glover, is among the most economically significant sap-sucking insect pests, inflicting substantial economic losses worldwide. Insecticides such as thiamethoxam, bifenthrin, and flonicamid are commonly used to manage this pest, despite the inherent risk of developing resistance. In this study, we investigated the evolution of insecticide resistance in A. gossypii after continuous selection with thiamethoxam, bifenthrin, and flonicamid over more than ten generations in a controlled laboratory environment. We assessed the fitness of resistant strains using an age-stage, two-sex life table approach, comparing them to a susceptible population. The results indicated that A. gossypii achieved resistance levels of 158.60-fold against thiamethoxam, 129.18-fold against bifenthrin, and 104.75-fold against flonicamid. Furthermore, life table analyses revealed that the developmental stages were significantly extended, while longevity decreased in all resistant strains compared to the susceptible population. Additionally, the net reproductive rate (R₀), fecundity, and reproductive days were notably reduced in the resistant cohorts when compared to the susceptible strain. Overall, these findings provide valuable insights into the laboratory-induced evolution of insecticide resistance and the associated fitness costs in A. gossypii when feeding on cotton plants. This information could be instrumental in formulating effective resistance management strategies to control this significant pest. Full article

(This article belongs to the Section Plant Protection and Biotic Interactions)

► Show Figures

Figure 1

25 pages, 405 KiB

Open AccessArticle

Chemical Composition, Antioxidant and Antimicrobial Activity of Piper carpunya and Simira ecuadorensis: A Comparative Study of Four Extraction Methods

by María del Cisne Guamán-Balcázar, Diana Hualpa, Garlet Infante, Luis Luzuriaga, José Luis Riofrío, Anderli Jarro, Estefany Lopez, Verónica Salas-Gomez, Rómulo Salazar, Jorge F. Reyes and Miguel A. Meneses

Plants 2025, 14(16), 2526; https://doi.org/10.3390/plants14162526 - 14 Aug 2025

Abstract

In this in vitro experimental study, we compared four extraction techniques -dynamic maceration (DME), ultrasound-assisted (UAE), microwave-assisted (MAE), and pressurized liquid extraction (PLE)- to obtain bioactive extracts from two native Ecuadorian plants, Piper carpunya and Simira ecuadorensis. The effect of extraction techniques was [...] Read more.

In this in vitro experimental study, we compared four extraction techniques -dynamic maceration (DME), ultrasound-assisted (UAE), microwave-assisted (MAE), and pressurized liquid extraction (PLE)- to obtain bioactive extracts from two native Ecuadorian plants, Piper carpunya and Simira ecuadorensis. The effect of extraction techniques was evaluated separately for each specie based on extraction yield, total phenolic content (TPC), antioxidant capacity (DPPH, ABTS, FRAP, and ORAC assays), antimicrobial activity, and chemical composition. All analyses were performed in triplicate and analyzed statistically (ANOVA, p < 0.05). UAE and MAE exhibited the highest extraction yield, while PLE provided extracts with the greatest TPC. However, UAE extracts, particularly for S. ecuadorensis, exhibited superior antioxidant capacity across assays. GC/MS analysis revealed alkanes as predominant constituents, along with minor phenolic and ester compounds. Antimicrobial activity was observed in both species, especially against Listeria monocytogenes and Pseudomonas aeruginosa, with UAE and MAE extracts being most effective. Compounds such as isoelemicin, phytol, and ethyl linolenate may contribute to the observed bioactivities. These findings highlight the potential of P. carpunya and S. ecuadorensis as natural sources of antioxidants and antimicrobials for food and pharmaceutical applications. Full article

(This article belongs to the Special Issue Analysis of Phenolic Compounds and Antioxidant Capacity in Native Plants: Characterization and Biological Effects)

15 pages, 1864 KiB

Open AccessArticle

Effects of Zinc and Organic Fertilizer Amendments, Applied Individually or in Combination, on Cadmium Uptake by Wheat Grown in Alkaline Soil

by Jiang Liu, Lingxuan Kong, Yanan Wan, Qi Wang, Zhong Zhuang and Huafen Li

Plants 2025, 14(16), 2525; https://doi.org/10.3390/plants14162525 - 13 Aug 2025

Abstract

Zinc (Zn) and organic fertilizer (OF) play a dual role in both promoting plant growth and modulating cadmium (Cd) uptake. However, the individual and combined effects of soil-applied Zn and OF on Cd accumulation in wheat remain insufficiently understood, with reported outcomes varying [...] Read more.

Zinc (Zn) and organic fertilizer (OF) play a dual role in both promoting plant growth and modulating cadmium (Cd) uptake. However, the individual and combined effects of soil-applied Zn and OF on Cd accumulation in wheat remain insufficiently understood, with reported outcomes varying from inhibition to promotion of Cd uptake. Therefore, this study systematically investigated the effect of Zn, organic fertilizer, and their combined treatment on the uptake dynamics of Cd and Zn in wheat plants across different growth stages. The pot culture experiments demonstrated that applying 20 mg/kg ZnSO₄ alone significantly reduced grain Cd content by 22.3% at the mature stage. Increasing the Zn dose to 40 mg/kg further enhanced the reduction, lowering Cd accumulation by 38.9% and decreasing Cd levels from 0.23 to 0.14 mg/kg. The application of 1% OF in alkaline soil enhanced soil Cd availability but did not significantly affect Cd accumulation in various wheat organs. The combination of Zn and organic fertilizer resulted in a relatively modest grain Cd reduction of 8.4–23.0%. Generally, Zn application alone was more effective in reducing Cd accumulation in wheat, while organic fertilizer may require careful use due to its Cd-mobilizing effect in alkaline soil. The combination of Zn and organic fertilizer showed limited benefits for Cd mitigation. Full article

► Show Figures

Figure 1

16 pages, 1570 KiB

Open AccessArticle

Examining the Role of Extraction Techniques and Regional Variability in the Antioxidant and Phytochemical Composition of Juglans regia L. Septa

by Jurgita Luksiene, Augusta Zevzikoviene, Jurga Andreja Kazlauskaite, Mindaugas Marksa, Daiva Majiene and Andrejus Zevzikovas

Plants 2025, 14(16), 2524; https://doi.org/10.3390/plants14162524 - 13 Aug 2025

Abstract

Walnut septa, traditionally discarded as waste in walnut processing because they primarily serve a structural function in the walnut fruit, have recently gained attention for their potential abundance of phenolic compounds, suggesting their overlooked value. This study aimed to optimise extraction parameters to [...] Read more.

Walnut septa, traditionally discarded as waste in walnut processing because they primarily serve a structural function in the walnut fruit, have recently gained attention for their potential abundance of phenolic compounds, suggesting their overlooked value. This study aimed to optimise extraction parameters to maximise the extraction yield of bioactive compounds and explore regional variations in antioxidant activity and chemical composition of Juglans regia L. septa. The experimental variables included extraction methods (maceration, dynamic maceration, ultrasound processing, and reflux extraction), temperature, solvent type (methanol, acetone, and ethanol), and the percentage of water in the solvent. The optimal conditions were determined based on the total phenolic content—reflux extraction using 60% ethanol as a solvent for a duration of 60 min. Samples from 12 different regions in Lithuania, Armenia, and Ukraine were analysed for their phenolic and proanthocyanidin content and antioxidant activity using the CUPRAC method. The total phenolic content ranged from 131.55 to 530.92 mg of gallic acid equivalents per g of dry weight of plant material (mg GAE/g dw), while the proanthocyanidin content varied from 1.14 to 7.65 mg of (–)-epicatechin equivalents per g dry weight of plant material (mg EE/g dw). Among all the regions studied, the Šiauliai sample demonstrated the highest concentrations of phenolic compounds, proanthocyanidins, and antioxidant activity, with statistically significant differences compared to the other samples (p < 0.05). These findings demonstrate that walnut septa are a valuable source of phenolic compounds and antioxidants, with significant potential for developing natural nutraceuticals and antioxidant products. Full article

(This article belongs to the Section Phytochemistry)

► Show Figures

Figure 1

51 pages, 4203 KiB

Open AccessReview

Carbon Dot Nanoparticles Synthesized from Horticultural Extracts for Postharvest Shelf-Life Extension of Fruits and Vegetables

by Tshiamo B. Leta, Jerry O. Adeyemi and Olaniyi A. Fawole

Plants 2025, 14(16), 2523; https://doi.org/10.3390/plants14162523 - 13 Aug 2025

Abstract

The increasing demand for sustainable food preservation technologies has spurred interest in green-synthesized carbon dots (CDs) derived from horticultural produce, positioning them as a promising nanomaterial for prolonging the shelf life of perishable food products. Most of these green approaches offer renewable, low-cost [...] Read more.

The increasing demand for sustainable food preservation technologies has spurred interest in green-synthesized carbon dots (CDs) derived from horticultural produce, positioning them as a promising nanomaterial for prolonging the shelf life of perishable food products. Most of these green approaches offer renewable, low-cost nanoparticles with excellent ultraviolet (UV) light barrier capabilities, antioxidant, and antimicrobial properties. These features help protect food products from the growth of foodborne pathogens and retard oxidative spoilage to extend their shelf life through edible coatings and packaging. To this end, this review critically explores current breakthroughs in biosynthesis, characterization, and application of CDs generated from different agricultural extracts, the mechanism of action, and possible synergistic effects when paired with other food preservation agents, aligning with circular economic principles. Scalability challenges, regulatory limitations, and potential future directions are all explored to present a comprehensive understanding of the topic, paving the way for innovative preservation methods in the food industry. Full article

(This article belongs to the Special Issue Advancing Sustainable Agriculture Through Nanomaterials: Challenges and Opportunities)

► Show Figures

Figure 1

13 pages, 1726 KiB

Open AccessArticle

Exploring Reproductive Timing in Olive Tree: Male Meiosis and Anthesis Events

by Emma Tedeschini, Fabio Orlandi and Marco Fornaciari

Plants 2025, 14(16), 2522; https://doi.org/10.3390/plants14162522 - 13 Aug 2025

Abstract

The timing of male meiosis plays a pivotal role in ensuring successful pollination and may represent a critical window during which environmental stressors can significantly impact reproductive outcomes. In anemophilous plants, both the quantity of microspores produced and the development of viable pollen [...] Read more.

The timing of male meiosis plays a pivotal role in ensuring successful pollination and may represent a critical window during which environmental stressors can significantly impact reproductive outcomes. In anemophilous plants, both the quantity of microspores produced and the development of viable pollen are particularly susceptible to external influences, such as fluctuating climatic conditions. This study undertakes a comprehensive analysis of reproductive features, focusing on the meiotic events of male gametogenesis and the phenological phases from the onset to full flowering in olive from central Italy. Utilizing a robust 11-year database (2012–2022), the research incorporates detailed micro- and macro-phenological observations alongside systematic pollen monitoring. The temporal regulation of male meiosis directs the phenological plasticity of the olive tree (Olea europaea L.) by transforming it into maladaptive phenological plasticity, effectively making the plant insensitive to thermal changes. This remarkable physiological trait underscores the resilience of this woody species to climate change. The results obtained will help to elucidate the interaction between climatic factors and reproductive dynamics, contributing valuable insights into the broader context of phenological responses to environmental changes. Full article

(This article belongs to the Section Plant Development and Morphogenesis)

► Show Figures

Figure 1

15 pages, 3733 KiB

Open AccessArticle

Enhancing Sugarcane Yield and Weed Control Sustainability with Degradable Film Mulching

by Xin Yuan, Rudan Li, Guolei Tang, Shaolin Yang and Jun Deng

Plants 2025, 14(16), 2521; https://doi.org/10.3390/plants14162521 - 13 Aug 2025

Abstract

A two-year field study evaluated biodegradable plastic film (BPF; thicknesses: 0.006, 0.008, and 0.010 mm) versus polyethylene film (PE; 0.010 mm) and no-mulch control on sugarcane yield and weed suppression. Key results demonstrated that 0.010 mm BPF significantly enhanced sugarcane emergence (CV [...] Read more.

A two-year field study evaluated biodegradable plastic film (BPF; thicknesses: 0.006, 0.008, and 0.010 mm) versus polyethylene film (PE; 0.010 mm) and no-mulch control on sugarcane yield and weed suppression. Key results demonstrated that 0.010 mm BPF significantly enhanced sugarcane emergence (CV = 5.07% in ratoon), reduced weed biomass by 70%, and increased perennial yield by 3.83% (+5.6 t ha⁻¹), while PE film decreased yield by 3.80%. Regression analysis identified the effective stem number, plant height, and stem diameter as primary yield predictors (R² = 0.996). Logistic models revealed that film mulching duration >119 days was critical for achieving high yields (>122.2 t ha⁻¹) and sustained weed control (R² = 0.81). These findings establish 0.010 mm BPF as an optimal sustainable alternative to PE film for enhancing sugarcane productivity. Full article

(This article belongs to the Special Issue Enhancing Soil Health and Crop Productivity Through Sustainable Agricultural Practices)

► Show Figures

Figure 1

22 pages, 5908 KiB

Open AccessArticle

The Effect of Far-Red Light on the Growth of Tobacco Leaves

by Lei Liu, Shujie Gai, Chuanke Liu, Zouguo Zeng, Xudong Tan, Jiawei Li and Zhi Zhou

Plants 2025, 14(16), 2520; https://doi.org/10.3390/plants14162520 - 13 Aug 2025

Abstract

To investigate how far-red (FR) light affects tobacco leaf growth, we established different light conditions, namely, CK: white (WL), T1: red (R), T2: red–white (R+WL) combination, T3: white–far-red (WL+FR) combination, and T4: white–red–far-red (WL+R+FR) combination; conducted supplemental light experiments on tobacco; and evaluated [...] Read more.

To investigate how far-red (FR) light affects tobacco leaf growth, we established different light conditions, namely, CK: white (WL), T1: red (R), T2: red–white (R+WL) combination, T3: white–far-red (WL+FR) combination, and T4: white–red–far-red (WL+R+FR) combination; conducted supplemental light experiments on tobacco; and evaluated the growth of tobacco leaves by determining the biomass, size of the leaves, etc. In addition, the auxin (IAA) content and expression of leaf growth-related genes were examined to further reveal the mechanism of the FR regulation of tobacco leaf growth. The results show a maximum reduction in leaf area size of more than 90% and in fresh dry mass of more than 85%, while the chlorophyll content increased by more than 28%. in tobacco leaves exposed to FR compared with those exposed to white light. Meanwhile, levels of auxin IAA were increased by 113% (T3) and 17% (T4) under far-red light treatment. The anatomical structure of the tobacco leaves showed that FR reduced the number of epidermal cells in the leaves but increased the cell size. Subsequent findings revealed that FR’s impact on leaf growth was mediated through the PHYB–PIF7–IAA signaling pathway, wherein it regulated cell division and growth-related genes. This substantiates that FR diminishes the tobacco leaf area by impeding cell division rather than inhibiting cell growth. In this study, we explored the effects of far-red (FR) light on tobacco leaf growth changes and constructed a model of the related signaling pathways. Our results reveal a novel mechanism by which far-red light regulates the growth of tobacco leaves, elucidating how far-red light affects their growth and response to shading conditions. This finding not only provides a scientific basis for the optimization of high-density tobacco planting but also helps to improve photosynthetic efficiency and yield, providing strong support for the sustainable development of tobacco farming. Full article

(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)

► Show Figures

Figure 1

20 pages, 3926 KiB

Open AccessArticle

Plant-Pollinator and Plant-Florivore Interactions in Two Savanna Species of Malpighiaceae

by Ludimila Juliele Carvalho-Leite and Helena Maura Torezan-Silingardi

Plants 2025, 14(16), 2519; https://doi.org/10.3390/plants14162519 - 13 Aug 2025

Viewed by 26

Abstract

Plant density influences interspecific interactions such as pollination and herbivory. In denser populations, pollinators find flowers more easily, increasing reproductive success and population growth. However, the same floral attractiveness also favors floral herbivory, a relationship described by Janzen and Connell as negative density [...] Read more.

Plant density influences interspecific interactions such as pollination and herbivory. In denser populations, pollinators find flowers more easily, increasing reproductive success and population growth. However, the same floral attractiveness also favors floral herbivory, a relationship described by Janzen and Connell as negative density dependence, considered an important mechanism for maintaining tropical diversity. This study analyzed the reproduction of Peixotoa tomentosa A. Juss. (Malpighiaceae) and Byrsonima intermedia A. Juss. (Malpighiaceae), considering population density and its influence on pollinator and herbivore attraction. The central hypothesis was that density affects fruit production. We conducted two treatments with both species: high density and low density in a preserved Brazilian savanna. We investigated fruit production, reproductive system, floral visitation rates, and the florivory rates of each species on each treatment. Our results showed that fruiting increased with density in both species. Peixotoa tomentosa is an agamospermous species, while B. intermedia is self-incompatible and relies exclusively on pollinators. Bees visited only B. intermedia, and the high-density treatment received more visits. Herbivores attacked more isolated P. tomentosa flowers. We concluded that density influences both pollination and herbivory, affecting plant reproduction, with effects mediated by the plant’s attractiveness in denser populations and by the size and quantity of flowers in single individuals. Full article

(This article belongs to the Special Issue Plant-Animal Interactions: Highlighting Effects of Plant Anatomy, Natural History, and Environmental Changes)

► Show Figures

Figure 1

Journal Menu

Journal Browser

Plants, Volume 14, Issue 16 (August-2 2025) – 94 articles

Further Information

Guidelines

MDPI Initiatives

Follow MDPI