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Biotechnological Use of Yeast for the Production of Value-Added Products
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Biotechnological Use of Yeast for the Production of Value-Added Products

A special issue of Sustainability (ISSN 2071-1050). This special issue belongs to the section "Sustainable Chemical Engineering and Technology".

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 12335

Special Issue Editors

Prof. Dr. Waldemar Rymowicz

E-Mail Website
Guest Editor
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Sciences, Wrocław University of Environmental and Life Sciences, Chełmońskiego St. 37, 51-630 Wrocław, Poland
Interests: Biotechnological use of yeast-containing glycerin waste, biosynthesis of organic acids, polyols and feed yeasts
Dr. Magdalena Wróbel-Kwiatkowska

E-Mail Website
Guest Editor
Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Sciences, Wrocław University of Environmental and Life Sciences, Chełmońskiego St. 37, 51-630 Wrocław, Poland
Interests: biotechnological modification of plants, biotechnological use of yeast for the kynurenic acid production

Special Issue Information

Dear Colleagues,

Yeasts remain the model organisms in the biotechnology, but they can also serve as a platform for the production of many value-added products. Yarrowia lipolytica- oleaginous yeast, is a species, which can be applied in genetic engineering technology, but it also possesses the high potential for the production of  many valuable and industrially important products.

This issue will describe the recent research concerning the use of Y. lipolytica for the production of sweetener erythritol from the waste substrates (i.e. glycerol) and kynurenic acid (KYNA), compound of medicinal importance, exhibiting antioxidant, anti-inflammatory, anti-atherosclerotic and neuromodulatory activity. The potency of application the yeast Y. lipolytica for the production of bioactive compounds and functional food will be discussed especially that in the light of the latest regulation yeast biomass of  Y. lipolytica can be treated as a novel food.

Prof. Dr. Waldemar Rymowicz
Dr. Magdalena Wróbel-Kwiatkowska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sustainability is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • waste glycerin
  • erythritol
  • kynurenic acid
  • Yarrowia lipolytica

Published Papers (5 papers)

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Research

9 pages, 487 KiB  
Article
Improved Production of Kynurenic Acid by Yarrowia lipolytica in Media Containing Different Honeys
by Magdalena Wróbel-Kwiatkowska, Waldemar Turski, Piotr Juszczyk, Agnieszka Kita and Waldemar Rymowicz
Sustainability 2020, 12(22), 9424; https://doi.org/10.3390/su12229424 - 12 Nov 2020
Cited by 9 | Viewed by 1658
Abstract
Y. lipolytica remains a nonpathogenic, unconventional yeast, which can be applied for the production of bioactive compounds. Our previous study confirmed the ability of yeast Yarrowia lipolytica to produce kynurenic acid (KYNA). Here, we investigated the effectiveness of KYNA production in cultures cultivated [...] Read more.
Y. lipolytica remains a nonpathogenic, unconventional yeast, which can be applied for the production of bioactive compounds. Our previous study confirmed the ability of yeast Yarrowia lipolytica to produce kynurenic acid (KYNA). Here, we investigated the effectiveness of KYNA production in cultures cultivated in medium containing honey of various origin, used as a source of carbon and energy. It was evidenced that the highest content of KYNA in culture broth (68 mg/L) and yeast biomass (542 mg/kg) was obtained when chestnut honey was used. The content of lipids and amino acids composition in yeast biomass producing KYNA was also determined. It was found that the composition of both amino acids and lipids in yeast biomass depended on the honey type used as a component of the medium. This finding revealed that supplementation of medium broth with honey may significantly affect the nutritional value of yeast biomass. The practical applicability of this finding requires further study. Full article
(This article belongs to the Special Issue Biotechnological Use of Yeast for the Production of Value-Added Products)
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12 pages, 956 KiB  
Article
Rye and Oat Agricultural Wastes as Substrate Candidates for Biomass Production of the Non-Conventional Yeast Yarrowia lipolytica
by Katarzyna Drzymała, Aleksandra Maria Mirończuk, Witold Pietrzak and Adam Dobrowolski
Sustainability 2020, 12(18), 7704; https://doi.org/10.3390/su12187704 - 18 Sep 2020
Cited by 29 | Viewed by 2985
Abstract
The aim of this study was to test rye straw, rye bran and oat bran hydrolysates as substrates for growth of the yeast Yarrowia lipolytica, a microorganism known to have large biotechnological potential. First, after the combined process of acid-enzymatic hydrolysis, the [...] Read more.
The aim of this study was to test rye straw, rye bran and oat bran hydrolysates as substrates for growth of the yeast Yarrowia lipolytica, a microorganism known to have large biotechnological potential. First, after the combined process of acid-enzymatic hydrolysis, the concentration and composition of fermentable monosaccharides in the obtained hydrolysates were analyzed. Glucose was the main sugar, followed by xylose and arabinose. Rye bran hydrolysate had the highest sugar content—80.8 g/L. The results showed that this yeast was able to grow on low-cost medium and produce biomass that could be used as a feed in the form of single cell protein. The biomass of yeast grown in oat bran hydrolysate was over 9 g/L after 120 h, with the biomass total yield and total productivity values of 0.141 g/g and 0.078 g/h, respectively. The protein contents in yeast biomass were in the range of 30.5–44.5% of dry weight. Results obtained from Y. lipolytica cultivated in rye bran showed high content of exogenous amino acid (leucine 3.38 g, lysine 2.93 g, threonine 2.31 g/100 g of dry mass) and spectrum of unsaturated fatty acid with predominantly oleic acid—59.28%. In conclusion, these results demonstrate that lignocellulosic agricultural waste, after hydrolysis, could be efficiently converted to feed-related yeast biomass. Full article
(This article belongs to the Special Issue Biotechnological Use of Yeast for the Production of Value-Added Products)
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15 pages, 2360 KiB  
Article
Overexpression of Citrate Synthase Increases Isocitric Acid Biosynthesis in the Yeast Yarrowia lipolytica
by Piotr Hapeta, Magdalena Rakicka-Pustułka, Piotr Juszczyk, Małgorzata Robak, Waldemar Rymowicz and Zbigniew Lazar
Sustainability 2020, 12(18), 7364; https://doi.org/10.3390/su12187364 - 8 Sep 2020
Cited by 10 | Viewed by 2535
Abstract
Yarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on the biosynthesis of citric and isocitric acid in Y. lipolytica. Two transformants of Y. lipolytica A101.1.31 [...] Read more.
Yarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on the biosynthesis of citric and isocitric acid in Y. lipolytica. Two transformants of Y. lipolytica A101.1.31 strain (efficient citric acid producer), overexpressing CIT1 or CIT2 gene (encoding proteins with citrate synthase activity), were constructed. The results revealed that overexpression of either of these genes enhances citrate synthase activity. Additionally, the cit1 knockout strain was unable to use propionate as the sole carbon source, which proves that CIT1 gene encodes a dual activity protein–citrate and 2-methylcitrate synthase. In the overexpressing mutants, a significant increase in isocitric acid biosynthesis was observed. Both CIT1 and CIT2 overexpressing strains produced citric and isocitric acid from vegetable oil in a ratio close to 1 (CA/ICA ratio for wild-type strain was 4.12). Full article
(This article belongs to the Special Issue Biotechnological Use of Yeast for the Production of Value-Added Products)
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13 pages, 551 KiB  
Article
Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica
by Ludwika Tomaszewska-Hetman, Waldemar Rymowicz and Anita Rywińska
Sustainability 2020, 12(17), 7122; https://doi.org/10.3390/su12177122 - 1 Sep 2020
Cited by 8 | Viewed by 2424
Abstract
The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources [...] Read more.
The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff. Full article
(This article belongs to the Special Issue Biotechnological Use of Yeast for the Production of Value-Added Products)
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17 pages, 1791 KiB  
Article
Alpha-Ketoglutaric Acid Production from a Mixture of Glycerol and Rapeseed Oil by Yarrowia lipolytica Using Different Substrate Feeding Strategies
by Anita Rywińska, Ludwika Tomaszewska-Hetman, Magdalena Rakicka-Pustułka, Piotr Juszczyk and Waldemar Rymowicz
Sustainability 2020, 12(15), 6109; https://doi.org/10.3390/su12156109 - 29 Jul 2020
Cited by 11 | Viewed by 2271
Abstract
The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention of many scientists as an alternative to its common chemical synthesis. The present study aimed to evaluate the effect of the feeding strategy of substrates, i.e., glycerol (G = 20 g·dm [...] Read more.
The microbiological biosynthesis of α-ketoglutaric acid (KGA) has recently captured the attention of many scientists as an alternative to its common chemical synthesis. The present study aimed to evaluate the effect of the feeding strategy of substrates, i.e., glycerol (G = 20 g·dm−3) and rapeseed oil (O = 20 g·dm−3), on yeast growth and the parameters of KGA biosynthesis by a wild strain Yarrowia lipolytica A-8 in fed-batch and repeated-batch cultures. The effectiveness of KGA biosynthesis was demonstrated to depend on thiamine concentration and the substrate feeding method. In the fed-batch culture incubated with 3 µg·dm−3 of thiamine and a substrate feeding variant 2G(_OGO), KGA was produced in the amount of 62.1 g·dm−3 at the volumetric production rate of 0.37 g·dm−3·h−1. These values of KGA production parameters were higher than these obtained in the control culture (with rapeseed oil only). During 10 cycles of the 1788-h repeated-batch culture carried out acc. to the feeding strategy 2G(_OGO), in the last 5 cycles the yeast produced from 55.6 to 58.2 g·dm−3 of KGA and maximally 2.9 g·dm−3 of the pyruvic acid as a by-product. Full article
(This article belongs to the Special Issue Biotechnological Use of Yeast for the Production of Value-Added Products)
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