Diagnosis, Prevention and Control in Avian Virus Infections

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Microbiology, Parasitology and Immunology".

Deadline for manuscript submissions: closed (8 August 2024) | Viewed by 5231

Special Issue Editors


E-Mail Website1 Website2
Guest Editor
National Institute for Agricultural and Veterinary Research, 2780-157 Oeiras, Portugal
Interests: animal viral diseases

E-Mail Website1 Website2
Guest Editor
National Institute for Agricultural and Veterinary Research, 2780-157 Oeiras, Portugal
Interests: animal viral diseases

Special Issue Information

Dear Colleagues,

Poultry farming is an activity aimed at the production of consumer goods, namely meat and eggs. Due to the high productivity of the birds, and the fact that they have a short biological cycle, high prolificacy and easy handling and experimentation, this activity is highly profitable. The overall performance of a poultry flock is dependent on various factors such as feed quality, poultry house management, and the circulation of pathogenic microorganisms, namely viruses. The presence of some viruses in a farm may cause diseases, leading to severe economic losses. When these viruses are zoonotic, the consequences may be even worse, since they may originate epidemics and pandemics in the human populations. Indeed, emerging infectious diseases often caused by zoonotic pathogens, including pathogenic zoonotic viruses, are responsible for a high percentage of mortality and morbidity observed worldwide, and most of the high impact outbreaks that have occurred in the past decades resulted from the contact of humans with livestock animals (i.e., Swine Influenza and AIV) and animal live markets (i.e., SARS-CoV-1 and SARS-CoV-2). However, not only poultry can be affected by avian viruses, since exotic pet birds are also susceptible to virus infections.

For the efficient control and prevention of infectious diseases, the identification of circulating pathogens is critical. Poultry vaccines are widely applied to prevent and control contagious diseases in poultry farms, and their use in production is aimed at avoiding or minimizing the emergence of clinical diseases at the farm level, thus increasing production and preventing zoonotic diseases. Vaccination is also applied in the framework of disease eradication programs under the official supervision of public veterinary services. However, due to selection pressures, as viruses change, the immune response also adapts, and therefore the existing vaccination programs may become inadequate in providing protection against disease. Thus, vaccine recommendations may change as new information regarding bird immunity and diseases is discovered.

We propose the Special Issue of "Diagnosis, Prevention and Control in Avian Virus Infections", with the aim to provide the knowledge needed to control avian viruses and to protect poultry farms and exotic pet birds from viral diseases. The objectives of this Special Issue are to provide new diagnostic tools to detect precociously avian viruses in birds and to make available new prevention and control measures to protect birds from viral diseases. Among the most common viral diseases affecting poultry are avian influenza, Newcastle disease, infectious bronchitis, avian reovirus, infectious bursal disease, avian encephalomyelitis, avian leukosis, chicken infectious anemia, fowl pox, egg drop syndrome, parvovirus infection, rotavirus infection, hemorrhagic enteritis, infectious laryngotracheitis and Marek's disease. Regarding exotic pet birds, there are also beak and feather disease, and polyomavirus and bornavirus infections. It is hoped that this Special Issue will further stimulate collaboration between scientists engaged in all aspects of this field of research.

Dr. Ana Margarida Henriques
Dr. Teresa Fagulha
Guest Editors

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Keywords

  • avian viral diseases
  • avian virus
  • viral diagnosis
  • serological diagnosis
  • next genera-tion sequencing
  • vaccination
  • disease control
  • phylogenetic analysis

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Published Papers (2 papers)

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Research

16 pages, 12504 KiB  
Article
Development of Virus-like Particle Plant-Based Vaccines against Avian H5 and H9 Influenza A Viruses
by Ola A. Elbohy, Munir Iqbal, Janet M. Daly and Stephen P. Dunham
Vet. Sci. 2024, 11(2), 93; https://doi.org/10.3390/vetsci11020093 - 18 Feb 2024
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Abstract
Avian influenza A virus (AIV) is a significant cause of mortality in poultry, causing substantial economic loss, particularly in developing countries, and has zoonotic potential. For example, highly pathogenic avian influenza (HPAI) viruses of the H5 subtype have been circulating in Egypt for [...] Read more.
Avian influenza A virus (AIV) is a significant cause of mortality in poultry, causing substantial economic loss, particularly in developing countries, and has zoonotic potential. For example, highly pathogenic avian influenza (HPAI) viruses of the H5 subtype have been circulating in Egypt for around two decades. In the last decade, H5N1 viruses of clade 2.2.1 have been succeeded by the antigenically distinct H5N8 clade 2.3.4.4b viruses. Furthermore, H9N2 viruses co-circulate with the H5N8 viruses in Egyptian poultry. It is widely recognised that effective vaccination against IAV requires a close antigenic match between the vaccine and viruses circulating in the field. Therefore, approaches to develop cost-effective vaccines that can be rapidly adapted to local virus strains are required for developing countries such as Egypt. In this project, the haemagglutinin (HA) proteins of Egyptian H5 and H9 viruses were expressed by transient transfection of plants (Nicotiana benthamiana). The formation of virus-like particles (VLPs) was confirmed by transmission electron microscopy. Mice were immunised with four doses of either H5 or H9 VLPs with adjuvant. Antibody and cellular immune responses were measured against the corresponding recombinant protein using ELISA and enzyme-linked immunosorbent assay (ELISpot), respectively. Chickens were immunised with one dose of H5 VLPs, eliciting HA-specific antibodies measured by ELISA and a pseudotyped virus neutralisation test using a heterologous H5 HA. In conclusion, plant-based VLP vaccines have potential for producing an effective vaccine candidate within a short time at a relatively low cost. Full article
(This article belongs to the Special Issue Diagnosis, Prevention and Control in Avian Virus Infections)
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11 pages, 1315 KiB  
Article
Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023
by Daniela Santos, Teresa Fagulha, Margarida Dias Duarte, Ana Duarte, Fernanda Ramos, Sílvia Carla Barros, Tiago Luís and Ana Margarida Henriques
Vet. Sci. 2023, 10(12), 693; https://doi.org/10.3390/vetsci10120693 - 7 Dec 2023
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Abstract
Avipoxvirus (APV), a linear dsDNA virus belonging to the subfamily Chordopoxvirinae of the family Poxviridae, infects more than 278 species of domestic and wild birds. It is responsible for causing avian pox disease, characterized by its cutaneous and diphtheric forms. With a [...] Read more.
Avipoxvirus (APV), a linear dsDNA virus belonging to the subfamily Chordopoxvirinae of the family Poxviridae, infects more than 278 species of domestic and wild birds. It is responsible for causing avian pox disease, characterized by its cutaneous and diphtheric forms. With a high transmission capacity, it can cause high economic losses and damage to the ecosystem. Several diagnostic methods are available, and bird vaccination can be an effective preventive measure. Ten APV-positive samples were analyzed to update the molecular characterization and phylogenetic analysis of viruses isolated in Portugal between 2017 and 2023. A P4b gene fragment was amplified using a PCR, and the nucleotide sequence of the amplicons was determined using Sanger sequencing. The sequences obtained were aligned using ClustalW, and a maximum likelihood phylogenetic tree was constructed. With this study, it was possible to verify that the analyzed sequences are distributed in subclades A1, A2, B1, and B3. Since some of them are quite similar to others from different countries and obtained in different years, it is possible to conclude that there have been several viral introductions in Portugal. Finally, it was possible to successfully update the data on Avipoxviruses in Portugal. Full article
(This article belongs to the Special Issue Diagnosis, Prevention and Control in Avian Virus Infections)
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