The effect of four sub-extracts prepared from the lyophilized hydroalcoholic bark of
Dipteryx alata (Leguminosae-Papilionoideae) dissolved in a methanol-water (80:20) mixture through a liquid-liquid partition procedure has been investigated against the neuromuscular blockade of the venom of the snake
Bothrops jararacussu. The active CH
2Cl
2 sub-extract has been extensively analyzed for its chemical constituents, resulting in the isolation of four lupane-type triterpenoids: lupeol (
1), lupenone (
2), 28-hydroxylup-20(29)-en-3-one (
3), betulin (
4), nine isoflavonoids: 8-
O-methylretusin (
5), 7-hydroxy-5,6,4’-trimethoxyisoflavone (
6), afrormosin (
8), 7-hydroxy-8,3’,4’-trimethoxyisoflavone (
9), 7,3’-dihydroxy-8,4’-dimethoxyisoflavone (
10), odoratin (
11), 7,8,3’-trihydroxy-4’-methoxyisoflavone (
13), 7,8,3’-trihydroxy-6,4’-dimethoxyisoflavone (
15), dipteryxin (
17), one chalcone: isoliquiritigenin (
7), one aurone: sulfuretin (
14) and three phenolic compounds: vanillic acid (
12),
vanillin (
16), and protocatechuic acid (
18). Their chemical structures were elucidated on the basis of spectroscopic analysis, including HRMS, 1D- and 2D-NMR techniques.
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