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Advances in the Monitoring and Analysis of Foodborne Pathogens
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Advances in the Monitoring and Analysis of Foodborne Pathogens

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 1409

Special Issue Editor

Dr. Wentao Zhang

E-Mail Website
Guest Editor
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
Interests: food safety; foodborne diseases; antimicrobials; food packaging; food contaminants; nanotechnology for food and agriculture; polyphenolic nanochemistry; nanozyme; nanobiotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, with the acceleration of economic globalization, food safety has become a worldwide public health hotspot. Foodborne pathogens, which cause great harm to human health and are a major hidden danger to food safety, are the primary cause of foodborne diseases and do not decrease or disappear with economic development and technological progress. Moreover, worldwide foodborne incidents occur frequently and have not been effectively controlled, indicating the food safety situation is still poor. In the 21st century, some rapid detection technologies for pathogenic microorganisms have been developed, such as radioimmunoassay and enzyme, fluorescence, time-resolved fluorescence, chemiluminescence, and bioluminescence immunoassays in immunology; nuclear acid probes; and polymerase chain reaction detection techniques. The development of relevant technologies provides an important tool for controlling the harm posed by foodborne pathogens, and this Special Issue therefore aims to present selected contributions on advances in the monitoring and analysis of foodborne pathogens by various advanced strategies.

Dr. Wentao Zhang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • foodborne pathogens
  • food analysis
  • detection
  • food quality
  • food safety
  • nanotechnology
  • device

Published Papers (2 papers)

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Research

17 pages, 1976 KiB  
Article
Identification of Protein Biomarkers for Differentiating Listeria monocytogenes Genetic Lineage III
by Basant Gomaa, Jingjun Lu, Hossam Abdelhamed, Michelle Banes, Olga Pechanova, Tibor Pechan, Mark A. Arick II, Attila Karsi and Mark L. Lawrence
Foods 2024, 13(9), 1302; https://doi.org/10.3390/foods13091302 - 24 Apr 2024
Viewed by 482
Abstract
Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne illness characterized by septicemia, meningitis, encephalitis, abortions, and occasional death in infants and immunocompromised individuals. L. monocytogenes is composed of four genetic lineages (I, II, III, and IV) and fourteen serotypes. The [...] Read more.
Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne illness characterized by septicemia, meningitis, encephalitis, abortions, and occasional death in infants and immunocompromised individuals. L. monocytogenes is composed of four genetic lineages (I, II, III, and IV) and fourteen serotypes. The aim of the current study was to identify proteins that can serve as biomarkers for detection of genetic lineage III strains based on simple antibody-based methods. Liquid chromatography (LC) with electrospray ionization tandem mass spectrometry (ESI MS/MS) followed by bioinformatics and computational analysis were performed on three L. monocytogenes strains (NRRL B-33007, NRRL B-33014, and NRRL B-33077), which were used as reference strains for lineages I, II, and III, respectively. Results from ESI MS/MS revealed 42 unique proteins present in NRRL B-33077 and absent in NRRL B-33007 and NRRL B-33014 strains. BLAST analysis of the 42 proteins against a broader panel of >80 sequenced strains from lineages I and II revealed four proteins [TM2 domain-containing protein (NRRL B-33077_2770), DUF3916 domain-containing protein (NRRL B-33077_1897), DNA adenine methylase (NRRL B-33077_1926), and protein RhsA (NRRL B-33077_1129)] that have no homology with any sequenced strains in lineages I and II. The four genes that encode these proteins were expressed in Escherichia coli strain DE3 and purified. Polyclonal antibodies were prepared against purified recombinant proteins. ELISA using the polyclonal antibodies against 12 L. monocytogenes lineage I, II, and III isolates indicated that TM2 protein and DNA adenine methylase (Dam) detected all lineage III strains with no reaction to lineage I and II strains. In conclusion, two proteins including TM2 protein and Dam are potentially useful biomarkers for detection and differentiation of L. monocytogenes lineage III strains in clinical, environmental, and food processing facilities. Furthermore, these results validate the approach of using a combination of proteomics and bioinformatics to identify useful protein biomarkers. Full article
(This article belongs to the Special Issue Advances in the Monitoring and Analysis of Foodborne Pathogens)
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19 pages, 3049 KiB  
Article
New Approach Methods to Assess the Enteropathogenic Potential of Strains of the Bacillus cereus Group, including Bacillus thuringiensis
by Arnaud Fichant, Rachelle Lanceleur, Salma Hachfi, Alexandra Brun-Barale, Anne-Louise Blier, Olivier Firmesse, Armel Gallet, Valérie Fessard and Mathilde Bonis
Foods 2024, 13(8), 1140; https://doi.org/10.3390/foods13081140 - 09 Apr 2024
Viewed by 643
Abstract
Bacillus cereus (Bc) is a wide group of Gram-positive and spore-forming bacteria, known to be the etiological agents of various human infections, primarily food poisoning. The Bc group includes enteropathogenic strains able to germinate in the digestive tract and to produce enterotoxins such [...] Read more.
Bacillus cereus (Bc) is a wide group of Gram-positive and spore-forming bacteria, known to be the etiological agents of various human infections, primarily food poisoning. The Bc group includes enteropathogenic strains able to germinate in the digestive tract and to produce enterotoxins such as Nhe, Hbl, and CytK. One species of the group, Bacillus thuringiensis (Bt), has the unique feature of producing insecticidal crystals during sporulation, making it an important alternative to chemical pesticides to protect crops from insect pest larvae. Nevertheless, several studies have suggested a link between the ingestion of pesticide strains and human cases of food poisoning, calling their safety into question. Consequently, reliable tools for virulence assessment are worth developing to aid decision making in pesticide regulation. Here, we propose complementary approaches based on two biological models, the human intestinal Caco-2 cell line and the insect Drosophila melanogaster, to assess and rank the enteric virulence potency of Bt strains in comparison with other Bc group members. Using a dataset of 48 Bacillus spp. strains, we showed that some Bc group strains, including Bt, were able to induce cytotoxicity in Caco-2 cells with concomitant release of IL-8 cytokine, a landmark of pro-inflammatory response. In the D. melanogaster model, we were able to sort a panel of 39 strains into four different classes of virulence, ranging from no virulence to strong virulence. Importantly, for the most virulent strains, mortality was associated with a loss of intestinal barrier integrity. Interestingly, although strains can share a common toxinotype, they display different degrees of virulence, suggesting the existence of specific mechanisms of virulence expression in vivo in the intestine. Full article
(This article belongs to the Special Issue Advances in the Monitoring and Analysis of Foodborne Pathogens)
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