A novel aerotolerant anaerobic bacterium (strain M4Ah
T) was isolated from a terrestrial mud volcano (Taman Peninsula, Russia). Cells were small, cell-wall-less, non-motile cocci, 0.32–0.65 μm in diameter. The isolate was a mesophilic, neutrophilic chemoorganoheterotroph, growing on carbohydrates (D-glucose, D-trehalose, D-ribose, D-mannose,
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A novel aerotolerant anaerobic bacterium (strain M4Ah
T) was isolated from a terrestrial mud volcano (Taman Peninsula, Russia). Cells were small, cell-wall-less, non-motile cocci, 0.32–0.65 μm in diameter. The isolate was a mesophilic, neutrophilic chemoorganoheterotroph, growing on carbohydrates (D-glucose, D-trehalose, D-ribose, D-mannose, D-xylose, D-maltose, D-lactose, D-cellobiose, D-galactose, D-fructose, and D-sucrose), proteinaceous compounds (yeast extract, tryptone), and pyruvate. Strain M4Ah
T tolerated 2% oxygen in the gas phase, was catalase-positive, and showed sustainable growth under microaerobic conditions. The dominant cellular fatty acids of strain M4Ah
T were C
16:0 and C
18:0. The G+C content of the genomic DNA was 32.42%. The closest phylogenetic relative of strain M4Ah
T was
Mariniplasma anaerobium from the family
Acholeplasmataceae (order
Acholeplasmatales, class
Mollicutes). Based on the polyphasic characterization of the isolate, strain M4Ah
T is considered to represent a novel species of a new genus, for which the name
Peloplasma aerotolerans gen. nov., sp. nov. is proposed. The type strain of
Peloplasma aerotolerans is M4Ah
T (=DSM 112561
T = VKM B-3485
T = UQM 41475
T). This is the first representative of the order
Acholeplasmatales, isolated from a mud volcano.
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