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Analytical Techniques in Pharmaceutical and Biomedical Analysis II
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Analytical Techniques in Pharmaceutical and Biomedical Analysis II

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 3725

Special Issue Editors

Dr. Franciszek Główka

E-Mail Website
Guest Editor
Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, Poznań, Poland
Interests: bioanalysis of drugs; metabolites and endogenous compounds using different analytical techniques, especially HPLC; pharmacokinetics; clinical pharmacokinetics; bioavailability and bioequivalence study
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Marta Karaźniewicz-Łada

E-Mail Website
Guest Editor
Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 3 Rokietnicka Str., 60-806 Poznań, Poland
Interests: bioanalysis; HPLC-MS/MS technique; method validation; pharmacokinetics; pharmacogenetics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development and validation of new analytical and bioanalytical methods play a critical role in drug discovery and therapeutic drug monitoring. The most common challenges that arise during method development are the use of an appropriate sample pretreatment technique for isolating a substance from the matrix and the choice of analytical method for the separation and final determination of the target compounds on the basis of their physicochemical properties.

Therefore, it is our pleasure to invite authors and members of their research groups to submit an article for a Special Issue of Molecules titled “Analytical Techniques in Pharmaceutical and Biomedical Analysis II”, for which we serve as Guest Editors. This Special Issue aims to cover research trends in the development of analytical and bioanalytical methods that are relevant to the isolation, identification, and determination of substances in biomedical and pharmaceutical matrices. Original research papers and reviews on interesting developments related to sample preparation, separation techniques, and spectroscopic methods, as well as any novel techniques for small molecules, peptides, monoclonal antibodies, aptamers, etc., are very welcome.

The submission deadline is 31 October 2023, but we strongly encourage authors to respond to confirm their contributions with their proposed titles by 30 April 2023.

All manuscripts will undergo comprehensive review according to standard journal procedures and policies.

Thank you for considering this invitation.

Dr. Franciszek Główka
Dr. Marta Karaźniewicz-Łada
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioanalysis
  • biopharmaceutics
  • HPLC
  • GC
  • electrophoresis
  • mass spectrometry
  • pharmacokinetics
  • pharmaceutical analysis
  • pharmaceutical formulation
  • plant materials
  • spectroscopy
  • validation

Published Papers (3 papers)

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Research

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20 pages, 902 KiB  
Article
Development and Validation of a UPLC-MS/MS Method for Therapeutic Drug Monitoring, Pharmacokinetic and Stability Studies of First-Line Antituberculosis Drugs in Urine
by Mohamed Abouzid, Katarzyna Kosicka-Noworzyń, Marta Karaźniewicz-Łada, Prakruti Rao, Nisha Modi, Yingda L. Xie, Scott K. Heysell, Anna Główka and Leonid Kagan
Molecules 2024, 29(2), 337; https://doi.org/10.3390/molecules29020337 - 9 Jan 2024
Cited by 1 | Viewed by 1020
Abstract
Tuberculosis (TB) remains one of the leading global causes of mortality. Several methods have been established to detect anti-TB agents in human plasma and serum. However, there is a notable absence of studies analyzing TB drugs in urine. Thus, our objective was to [...] Read more.
Tuberculosis (TB) remains one of the leading global causes of mortality. Several methods have been established to detect anti-TB agents in human plasma and serum. However, there is a notable absence of studies analyzing TB drugs in urine. Thus, our objective was to validate a method for quantifying first-line anti-TB agents: isoniazid (INH), pyrazinamide (PZA), ethambutol (ETH), and rifampicin (RIF), along with its metabolite 25-desacetylrifampicin, and degradation products: rifampicin quinone and 3-formyl-rifampicin in 10 µL of urine. Chromatographic separation was achieved using a Kinetex Polar C18 analytical column with gradient elution (5 mM ammonium acetate and acetonitrile with 0.1% formic acid). Mass spectrometry detection was carried out using a triple-quadrupole tandem mass spectrometer operating in positive ion mode. The lower limit of quantification (LLOQ) was 0.5 µg/mL for INH, PZA, ETH, and RIF, and 0.1 µg/mL for RIF’s metabolites and degradation products. The method was validated following FDA guidance criteria and successfully applied to the analysis of the studied compounds in urine of TB patients. Additionally, we conducted a stability study of the anti-TB agents under various pH and temperature conditions to mimic the urine collection process in different settings (peripheral clinics or central laboratories). Full article
(This article belongs to the Special Issue Analytical Techniques in Pharmaceutical and Biomedical Analysis II)
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15 pages, 2988 KiB  
Article
Preanalytical Quality Evaluation of Citrate Evacuated Blood Collection Tubes—Ultraviolet Molecular Absorption Spectrometry Confronted with Ion Chromatography
by Nataša Gros and Tjaša Stopar
Molecules 2023, 28(23), 7735; https://doi.org/10.3390/molecules28237735 - 23 Nov 2023
Viewed by 926
Abstract
We previously enabled a direct insight into the quality of citrate anticoagulant tubes before their intended use for specimen collection by introducing an easy-to-perform UV spectrometric method for citrate determination on a purified water model. The results revealed differences between the tubes of [...] Read more.
We previously enabled a direct insight into the quality of citrate anticoagulant tubes before their intended use for specimen collection by introducing an easy-to-perform UV spectrometric method for citrate determination on a purified water model. The results revealed differences between the tubes of three producers, Greiner BIO-ONE (A), LT Burnik (B), and BD (C). It became apparent that tubes C contain an additive, which absorbs light in the ultraviolet range and prevents reliable evaluation of citrate anticoagulant concentration with the suggested method. In this research, we re-evaluate the quality of citrate-evacuated blood collection tubes by complementing UV spectrometry with ion chromatography. (1) Comparable results were obtained for tubes B at 220 nm. (2) Citrate concentrations determined with ion chromatography were lower for tubes A and C. Chromatograms reveal additional peaks for both. (3) Influences of heparin on absorption spectra and chromatograms of citrate were studied. Some similarities with the shape of the anticoagulant spectra of tubes A and C were observed, and the lithium heparin peak in chromatograms is close to them, but a confident judgment was not possible. (4) Contamination of anticoagulant solution with potassium, magnesium, and calcium was confirmed for all the brands, and contamination with lithium for B and C. Full article
(This article belongs to the Special Issue Analytical Techniques in Pharmaceutical and Biomedical Analysis II)
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32 pages, 975 KiB  
Review
Analytical and Clinical Validation of Assays for Volumetric Absorptive Microsampling (VAMS) of Drugs in Different Blood Matrices: A Literature Review
by Rhea Veda Nugraha, Vycke Yunivita, Prayudi Santoso, Aliya Nur Hasanah, Rob E. Aarnoutse and Rovina Ruslami
Molecules 2023, 28(16), 6046; https://doi.org/10.3390/molecules28166046 - 14 Aug 2023
Cited by 2 | Viewed by 1350
Abstract
Volumetric absorptive microsampling (VAMS) is the newest and most promising sample-collection technique for quantitatively analyzing drugs, especially for routine therapeutic drug monitoring (TDM) and pharmacokinetic studies. This technique uses an absorbent white tip to absorb a fixed volume of a sample (10–50 µL) [...] Read more.
Volumetric absorptive microsampling (VAMS) is the newest and most promising sample-collection technique for quantitatively analyzing drugs, especially for routine therapeutic drug monitoring (TDM) and pharmacokinetic studies. This technique uses an absorbent white tip to absorb a fixed volume of a sample (10–50 µL) within a few seconds (2–4 s), is more flexible, practical, and more straightforward to be applied in the field, and is probably more cost-effective than conventional venous sampling (CVS). After optimization and validation of an analytical method of a drug taken by VAMS, a clinical validation study is needed to show that the results by VAMS can substitute what is gained from CVS and to justify implementation in routine practice. This narrative review aimed to assess and present studies about optimization and analytical validation of assays for drugs taken by VAMS, considering their physicochemical drug properties, extraction conditions, validation results, and studies on clinical validation of VAMS compared to CVS. The review revealed that the bio-analysis of many drugs taken with the VAMS technique was optimized and validated. However, only a few clinical validation studies have been performed so far. All drugs that underwent a clinical validation study demonstrated good agreement between the two techniques (VAMS and CVS), but only by Bland–Altman analysis. Only for tacrolimus and mycophenolic acid were three measurements of agreement evaluated. Therefore, VAMS can be considered an alternative to CVS in routine practice, especially for tacrolimus and mycophenolic acid. Still, more extensive clinical validation studies need to be performed for other drugs. Full article
(This article belongs to the Special Issue Analytical Techniques in Pharmaceutical and Biomedical Analysis II)
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