Herein, we report the synthesis and characterization of a novel Schiff base ligand, (Z)-5-((pyren-1-ylmethylene)amino)-2,4-dihydro-3H-pyrazol-3-one (
PMDP). The characterization of ligand
PMDP was carried out using ESI-MS,
1H NMR, and UV–Visible spectroscopic techniques. As a probe,
PMDP displayed a detectable, colorimetric colour
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Herein, we report the synthesis and characterization of a novel Schiff base ligand, (Z)-5-((pyren-1-ylmethylene)amino)-2,4-dihydro-3H-pyrazol-3-one (
PMDP). The characterization of ligand
PMDP was carried out using ESI-MS,
1H NMR, and UV–Visible spectroscopic techniques. As a probe,
PMDP displayed a detectable, colorimetric colour shift in the presence of Cu
2+ and Fe
2+ ions. The solution was seen to have a light brown colour and to exhibit a fluorometric “turn off” response when Cu
2+ and Fe
2+ ions were present in a DMSO solution (HEPES 0.01 M, pH = 7.4) at room temperature. Job’s plot revealed that the
PMDP binding ratio to Cu
2+ and Fe
2+ ions was in 1:2 ratio. In contrast to the other metal ions (Cd
2+, Mn
2+, Co
2+, Na
+, Ni
2+, Cu
+, Fe
3+, Hg
2+, Mg
2+, Zn
2+, K
+, and V
5+), the synthesised probe showed exceptional sensitivity and selectivity for detecting Cu
2+ and Fe
2+ metal ions. The results indicate that the detection limits for Cu
2+ and Fe
2+ are 0.42 μM and 0.51 μM, respectively. Furthermore,
PMDP was efficiently utilised for the quantitative analysis of Cu
2+ and Fe
2+ in real water samples, RGB colour values in smart phones, logic gate construction, and cell imaging in HeLa cells.
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