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Fermentation, Volume 8, Issue 3

2022 March - 45 articles

Cover Story: Guanidinoacetate is a naturally occurring amino acid derivative, which is widely used in feed additives and the pharmaceutical industry. The application of GAA is limited by the inefficient production of GAA by chemical synthesis. In this study, a biological approach is developed for food safety GAA production via whole-cell biocatalysis by the generally regarded as safe (GRAS) bacterium Bacillus subtilis. We introduced a heterologous arginine: glycine amidinotransferase (AgaT) into B. subtilis and optimized its expression level. In order to increase the production, we optimized the natural ornithine cycle in B. subtilis. Here, we provide a new method for the biosynthesis of GAA. View this paper.
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Articles (45)

  • Article
  • Open Access
7 Citations
3,921 Views
13 Pages

A thermotolerant Enterococcus faecium s6 strain exhibited homoferementative lactic acid (LA) production at high xylose concentration (≥50 g/L). In batch fermentation at 45 °C and controlled pH of 6.5, strain E. faecium s6 produced up to 72.9 g...

  • Article
  • Open Access
41 Citations
15,788 Views
16 Pages

The aim of the present study was to investigate the feasibly of using traditional milk kefir grains for the production of water kefir-like beverages and assess the changes in the physicochemical characteristics and the microbial populations of the fe...

  • Article
  • Open Access
11 Citations
3,644 Views
10 Pages

Revalorization of Cava (Spanish Sparkling Wine) Lees on Sourdough Fermentation

  • Alba Martín-Garcia,
  • Montserrat Riu-Aumatell and
  • Elvira López-Tamames

Cava lees are a sparkling wine by-product formed of dead microorganisms, tartaric acid and other inorganic compounds, with a potential for enhancing microbial growth. Lees are rich in antioxidant compounds as well as β-glucans and mannoproteins....

  • Article
  • Open Access
10 Citations
4,669 Views
13 Pages

Enhancement of Labneh Quality by Laser-Induced Modulation of Lactocaseibacillus casei NRRL B-1922

  • Fouad M. F. Elshaghabee,
  • Ahmed El-Hussein and
  • Mahmoud S. M. Mohamed

Labneh is positioned in the top ranking of the bestselling dairy products all over the world due to its health benefits and delicious taste. Labneh production depends mainly on probiotic bacteria in the fermentation of milk. Probiotic bacteria have m...

  • Article
  • Open Access
17 Citations
8,307 Views
22 Pages

Effect of Feed Supplement Containing Dried Kratom Leaves on Apparent Digestibility, Rumen Fermentation, Serum Antioxidants, Hematology, and Nitrogen Balance in Goats

  • Pin Chanjula,
  • Juraithip Wungsintaweekul,
  • Rawee Chiarawipa,
  • Adirek Rugkong,
  • Benjamad Khonkhaeng,
  • Chanon Suntara and
  • Anusorn Cherdthong

The objectives of the present study were to examine the influence of supplementation with dried kratom leaf (DKTL) on the performance, hematology, and nitrogen balance in goats. Four 12-month-old male crossbred (Thai Native x Anglo Nubian) goats with...

  • Article
  • Open Access
6 Citations
4,535 Views
11 Pages

Modelling of the Simultaneous Saccharification and Fermentation for a Pine Sawdust Biorefinery

  • Carolina Mónica Mendieta,
  • Julia Kruyeniski,
  • Fernando Esteban Felissia and
  • María Cristina Area

The decline in world oil reserves evidences the need to diversify the sources of equivalent raw materials. The use of biomass is one of the most explored alternatives. This work evaluates the second-generation bioethanol (2G) production from a pine s...

  • Article
  • Open Access
22 Citations
4,328 Views
20 Pages

Sustainable and Eco-Friendly Conversions of Olive Mill Wastewater-Based Media by Pleurotus pulmonarius Cultures

  • Ilias Diamantis,
  • Eirini-Maria Melanouri,
  • Marianna Dedousi,
  • Ioanna Panagopoulou,
  • Seraphim Papanikolaou,
  • Nikolaos G. Stoforos and
  • Panagiota Diamantopoulou

Pleurotus pulmonarius mushroom was cultivated on liquid cultures with olive mill wastewaters (OMWs) of initial phenolic compound concentrations of 0 (control), 1, 2 and 3 g/L and glucose at initial concentrations of 40 and 60 g/L. The ability of the...

  • Article
  • Open Access
34 Citations
7,685 Views
22 Pages

Health Benefits of Postbiotics Produced by E. coli Nissle 1917 in Functional Yogurt Enriched with Cape Gooseberry (Physalis peruviana L.)

  • Mohamed Samir Darwish,
  • Longxin Qiu,
  • Mohamed A. Taher,
  • Ahmed A. Zaki,
  • Noha A. Abou-Zeid,
  • Dawood H. Dawood,
  • Ola M. A. K. Shalabi,
  • Ebtihal Khojah and
  • Asmaa A. Elawady

Changes in the activities of antimicrobial, antitumor, and antioxidant properties of postbiotics (YCG) are related to changes in the composition of phenolic compounds. Antimicrobial activity was found to be highest in postbiotic (YCG-7) against P. ae...

  • Editorial
  • Open Access
4 Citations
2,957 Views
3 Pages

In the food and beverage industries, implementing novel methods using digital technologies such as artificial intelligence (AI), sensors, robotics, computer vision, machine learning (ML), and sensory analysis using augmented reality (AR) has become c...

  • Article
  • Open Access
7 Citations
8,431 Views
20 Pages

Can Supplemented Skim Milk (SKM) Boost Your Gut Health?

  • Nelson Mota de Carvalho,
  • Diana Luazi Oliveira,
  • Célia Maria Costa,
  • Manuela Pintado and
  • Ana Raquel Madureira

The incorporation of functional ingredients, such as prebiotics and probiotics in food matrices, became a common practice in the human diet to improve the nutritional value of the food product itself. Worldwide, skim milk (SKM) is one of the most con...

  • Article
  • Open Access
31 Citations
10,082 Views
15 Pages

Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids

  • Kamran Jawed,
  • Victor Uhunoma Irorere,
  • Rajesh Reddy Bommareddy,
  • Nigel P. Minton and
  • Katalin Kovács

The facultative chemolithoautotroph Cupriavidus necator H16 is able to grow aerobically either with organic substrates or H2 and CO2 s and it can accumulate large amounts of (up to 90%) poly (3-hydroxybutyrate), a polyhydroxyalkanoate (PHA) biopolyme...

  • Article
  • Open Access
12 Citations
5,684 Views
22 Pages

Microbial Quality and Growth Dynamics in Shameta: A Traditional Ethiopian Cereal-Based Fermented Porridge

  • Daniel A. Kitessa,
  • Ketema Bacha,
  • Yetenayet B. Tola,
  • Mary Murimi,
  • Soressa Gershe and
  • Meseret Guta

Shameta is a traditional, Ethiopian, cereal-based fermented porridge exclusively prepared for lactating mothers. The aim of this study was to determine the microbial quality of Shameta samples collected from households of lactating mothers and to det...

  • Review
  • Open Access
18 Citations
8,583 Views
22 Pages

Looking inside Mexican Traditional Food as Sources of Synbiotics for Developing Novel Functional Products

  • Edgar Torres-Maravilla,
  • Vianey Méndez-Trujillo,
  • Natalia C. Hernández-Delgado,
  • Luis G. Bermúdez-Humarán and
  • Diana Reyes-Pavón

Currently, emerging alimentary alternatives are growing, leading to the consumption of natural products including bio, fermented, and traditional foods. The studies over functional properties of food matrices and their derived compounds have resulted...

  • Article
  • Open Access
18 Citations
4,538 Views
15 Pages

Postbiotics are functional bioactive substances manufactured during fermentation in a food matrix, which can be used to improve human health, but their influence on the adhesion potential and physicochemical cell surface of probiotics is still unclea...

  • Article
  • Open Access
6 Citations
3,550 Views
10 Pages

Recovery and Purification of Fumaric Acid from Fermented Oil Palm Empty Fruit Bunches Using a Simple Two-Stage Precipitation Procedure

  • Antika Boondaeng,
  • Nattaporn Khanoonkon,
  • Pilanee Vaithanomsat,
  • Waraporn Apiwatanapiwat,
  • Chanaporn Trakunjae,
  • Phornphimon Janchai and
  • Nanthavut Niyomvong

Oil palm empty fruit bunches (EFBs) are an attractive lignocellulosic material that can be used as a cheap renewable feedstock to produce organic acids and many other value-added products. This research is aimed at investigating the potential of stea...

  • Article
  • Open Access
18 Citations
6,902 Views
18 Pages

The Volatile Compounds and Aroma Description in Various Rhizopus oligosporus Solid-State Fermented and Nonfermented Rice Bran

  • Retno Dwi Astuti,
  • Dwi Larasatie Nur Fibri,
  • Dody Dwi Handoko,
  • Wahyudi David,
  • Slamet Budijanto,
  • Hitoshi Shirakawa and
  • Ardiansyah

Rice bran is known to have beneficial nutrients. Current studies suggest that solid-state fermentation affects the rice bran’s volatile profile. The aim of this study is to identify the volatile compounds and aroma description of fermented and...

  • Article
  • Open Access
18 Citations
4,447 Views
17 Pages

Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

  • Muhammad Hariadi Nawawi,
  • Khairul Izdihar Ismail,
  • Norazliza Sa’ad,
  • Rosfarizan Mohamad,
  • Paridah Md Tahir,
  • Ainun Zuriyati Asa’ari and
  • Wan Zuhainis Saad

An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central compos...

  • Article
  • Open Access
12 Citations
10,412 Views
10 Pages

Effects of Fermented Vegetable Consumption on Human Gut Microbiome Diversity—A Pilot Study

  • Kerstin Thriene,
  • Sina S. Hansen,
  • Nadine Binder and
  • Karin B. Michels

The interest in fermented food products has been increasing in recent years. Details about their microbial composition and the effects of their consumption on the human gut microbiome are of particular interest. However, evidence regarding their pote...

  • Review
  • Open Access
43 Citations
12,184 Views
29 Pages

Novel Developments on Stimuli-Responsive Probiotic Encapsulates: From Smart Hydrogels to Nanostructured Platforms

  • Andres J. Garcia-Brand,
  • Valentina Quezada,
  • Cristina Gonzalez-Melo,
  • Angie D. Bolaños-Barbosa,
  • Juan C. Cruz and
  • Luis H. Reyes

Biomaterials engineering and biotechnology have advanced significantly towards probiotic encapsulation with encouraging results in assuring sufficient bioactivity. However, some major challenges remain to be addressed, and these include maintaining s...

  • Review
  • Open Access
11 Citations
5,059 Views
18 Pages

Bio-Based Processes for Material and Energy Production from Waste Streams under Acidic Conditions

  • Zeynep Cetecioglu,
  • Merve Atasoy,
  • Adam Cenian,
  • Gaweł Sołowski,
  • Janja Trček,
  • Aysenur Ugurlu and
  • Jana Sedlakova-Kadukova

The revolutionary transformation from petrol-based production to bio-based production is becoming urgent in line with the rapid industrialization, depleting resources, and deterioration of the ecosystem. Bio-based production from waste-streams is off...

  • Article
  • Open Access
9 Citations
4,984 Views
11 Pages

Biosynthesis of Guanidinoacetate by Bacillus subtilis Whole-Cell Catalysis

  • Kun Yan,
  • Rongzhen Tian,
  • Linpei Zhang,
  • Xueqin Lv,
  • Long Liu and
  • Yanfeng Liu

Guanidinoacetate (GAA) is a naturally occurring amino acid derivative and the direct precursor of creatine, which is widely used in feed additives and the pharmaceutical industry. The current industrial synthesis of GAA is based on chemical methods,...

  • Article
  • Open Access
12 Citations
8,358 Views
12 Pages

Bioethanol Production from Spent Sugar Beet Pulp—Process Modeling and Cost Analysis

  • Damjan Vučurović,
  • Bojana Bajić,
  • Vesna Vučurović,
  • Rada Jevtić-Mučibabić and
  • Siniša Dodić

Global economic development has led to the widespread use of fossil fuels, and their extensive use has resulted in increased environmental pollution. As a result, significantly more attention is being paid to environmental issues and alternative rene...

  • Article
  • Open Access
8 Citations
3,454 Views
13 Pages

High antioxidant levels in food are gradually becoming popular because of enhanced risk of oxidative stress in humans. Bread wheat is rich in vital antioxidants, but a major portion of its bioactive compounds are not available to humans. This study w...

  • Article
  • Open Access
29 Citations
6,339 Views
13 Pages

Production of a Yogurt Drink Enriched with Golden Berry (Physalispubescens L.) Juice and Its Therapeutic Effect on Hepatitis in Rats

  • Magdy Ramadan Shahein,
  • El Sayed Hassan Atwaa,
  • Hanan A. Radwan,
  • Abdelmoneim Ahmed Elmeligy,
  • Amin A. Hafiz,
  • Ashraf Albrakati and
  • Ehab Kotb Elmahallawy

Fermented dairy products have been associated with multiple health benefits. The present study aimed to produce a functional yogurt drink fortified with golden berry juice and assess its therapeutic effect on hepatitis rats. Thirty male albino rats w...

  • Article
  • Open Access
2 Citations
3,898 Views
13 Pages

Stimulation of Toll-like receptor 2 (TLR2) on epidermal keratinocytes results in the tightening of cell–cell junctions between keratinocytes; therefore, appropriate agonists for TLR2 could be promising ingredients for cosmetics. However, a meth...

  • Article
  • Open Access
5 Citations
4,117 Views
20 Pages

Despite the importance of the biodegradability of lignocellulose biomass, few studies have evaluated the lignocellulose biomass digestion kinetics and modeling of the process. Anaerobic digestion (AD) is a mature energy production technique in which...

  • Article
  • Open Access
5 Citations
3,495 Views
12 Pages

Condensed tannins (CT) have been observed to reduce enteric CH4 production when added to ruminant diets. However, high concentrations of CT in forages such as sericea lespedeza (SL; Lespedeza cuneata (Dum. Cours.) G. Don) may depress nutrient digesti...

  • Article
  • Open Access
13 Citations
6,455 Views
12 Pages

Polysaccharide Extracts Derived from Defloration Waste of Fruit Pitaya Regulates Gut Microbiota in a Mice Model

  • Bao-Hong Lee,
  • Kung-Ting Hsu,
  • You-Zuo Chen,
  • You-Lin Tain,
  • Chih-Yao Hou,
  • Yong-Chong Lin and
  • Wei-Hsuan Hsu

Flower thinning is often used during the planting of fruit trees to improve fruit quality and promote large fruit. Flower buds become an agricultural by-product of the planting process. Pitaya (Hylocereus undatus) is a popular fruit in many tropical...

  • Article
  • Open Access
9 Citations
3,937 Views
22 Pages

Pyrolytic aqueous condensate (PAC) might serve as a cost-effective substrate for microbial malic acid production, as it is an unused side stream of the fast pyrolysis of lignocellulosic biomass that contains acetol and acetate as potential carbon sou...

  • Article
  • Open Access
23 Citations
5,240 Views
14 Pages

A Natural Technology for Vacuum-Packaged Cooked Sausage Preservation with Potentially Postbiotic-Containing Preservative

  • Aloizio Lemos de Lima,
  • Carlos Alberto Guerra,
  • Lucas Marques Costa,
  • Vanessa Sales de Oliveira,
  • Wilson José Fernandes Lemos Junior,
  • Rosa Helena Luchese and
  • André Fioravante Guerra

In this study, a potentially postbiotic-containing preservative (PPCP) was produced in an axenic fermentation system with Lacticaseibacillus paracasei DTA 83 as a natural technology alternative for vacuum-packaged cooked sausage preservation. Cooked...

  • Article
  • Open Access
3 Citations
2,896 Views
11 Pages

This study focused on applying a patented medium-chain fatty acids (MCFAs) mixture at the end of alcoholic fermentation and monitoring its residues. MCFAs are a promising agent that has the potential to increase the efficiency of sulfur dioxide and u...

  • Article
  • Open Access
11 Citations
5,056 Views
16 Pages

Characterization of Green Manure Sunn Hemp Crop Silage Prepared with Additives: Aerobic Instability, Nitrogen Value, and In Vitro Rumen Methane Production

  • Chatchai Kaewpila,
  • Waroon Khota,
  • Pongsatorn Gunun,
  • Piyawit Kesorn,
  • Thachawech Kimprasit,
  • Chaweng Sarnklong and
  • Anusorn Cherdthong

Sunn hemp (SH, Crotalaria juncea, L.) is a tropical multiple-purpose legume. The green manure SH (GMSH) crop might display protein ecology in sustaining ruminants; however, its silage features remain unclear. To efficiently prepare GMSH crop silage,...

  • Article
  • Open Access
20 Citations
4,274 Views
12 Pages

To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an in vitro cumulative gas production experiment was applied with mixed rumen microorganism to compare rumen fermentation...

  • Article
  • Open Access
11 Citations
6,134 Views
29 Pages

Investigating a Stirred Bioreactor: Impact of Evolving Fermentation Broth Pseudoplastic Rheology on Mixing Mechanisms

  • M. Constanza Sadino-Riquelme,
  • José Rivas,
  • David Jeison,
  • Andrés Donoso-Bravo and
  • Robert E. Hayes

The culture medium in many fermentations is a non-Newtonian fluid. In bacterial alginate batch production, the broth becomes more pseudoplastic as the alginate concentration increases, which impairs the mixing process. This work characterizes the eff...

  • Article
  • Open Access
3 Citations
3,819 Views
22 Pages

Cytoprotective Effects of Lactobacilli on Mouse Epithelial Cells during Salmonella Infection

  • Guangzhi Zhang,
  • Abdul Raheem,
  • Xintao Gao,
  • Jianwei Zhang,
  • Lijun Shi,
  • Mingyan Wang,
  • Ming Li,
  • Yajie Yin,
  • Shaohan Li and
  • Tong Qin
  • + 4 authors

Treatment of common pathogens, such as Salmonella species, Escherichia coli, Staphylococcus aureus, etc., is a big challenge for a practitioner. Antibiotics’ side effects during their application for the treatment of infectious diseases sh...

  • Article
  • Open Access
27 Citations
9,363 Views
10 Pages

Agricultural food waste is rich in cellulosic and non-cellulosic fermentable substance. In this study, we investigated the bioconversion of pineapple waste cell wall sugars into bioethanol by simultaneous saccharification and fermentation using Sacch...

  • Article
  • Open Access
6 Citations
3,482 Views
16 Pages

Printing and dyeing wastewater is characterized with complex water quality and poor biodegradability. In this study, a pilot-scale anaerobic baffled reactor (ABR) with packing was verified to effectively degrade the complex organic pollutants in the...

  • Article
  • Open Access
2 Citations
2,884 Views
16 Pages

Immune Response and Fatty Acid Profile of Eggs from Laying Hens Fed Fermented Feed Rich in Polyunsaturated Fatty Acids

  • Viera Karaffová,
  • Dagmar Mudroňová,
  • Boris Semjon,
  • Tatiana Klempová,
  • Ondrej Slaný,
  • Milan Čertík,
  • Jozef Nagy,
  • Martin Bartkovský and
  • Slavomír Marcinčák

Western diets are dominated by the consumption of chemically modified foods, characterized by a deficiency of n-3 polyunsaturated fatty acids (PUFAs). Lack of n-3 PUFAs is also present in livestock feed, which negatively affects livestock health, inc...

  • Article
  • Open Access
7 Citations
10,611 Views
12 Pages

Background: Soy sauce is applied as a seasoning in daily life in East Asia. High amounts of salt in soy sauce can inhibit microbial growth in its production and preservation. However, the elevated salt content in food might increase the health risk....

  • Article
  • Open Access
8 Citations
5,056 Views
10 Pages

Acid tolerance of the lactic acid bacterium (LAB) is crucially important for the production of free lactic acid as a chemical monomer by simplified purification steps. This study conducts both metabolic modification and adaptive evolution approaches...

  • Article
  • Open Access
3 Citations
3,094 Views
17 Pages

The emerging elicitor protein PeBA1, extracted from Bacillus amyloliquefaciens NC6, was tested against the cabbage aphid (Brevicoryne brassicae) for its biocontrol potential. Its effects on the survival, lifespan, immature development, adult reproduc...

  • Article
  • Open Access
11 Citations
3,347 Views
24 Pages

Using the Mixed Culture of Fodder Mallow (Malva verticillata L.) and White Sweet Clover (Melilotus albus Medik.) for Methane Production

  • Antonín Kintl,
  • Igor Huňady,
  • Jiří Holátko,
  • Tomáš Vítěz,
  • Tereza Hammerschmiedt,
  • Martin Brtnický,
  • Vladěna Ondrisková and
  • Jakub Elbl

The ever-growing number of biogas plants also leads to an increasing demand for suitable, alternative plant substrates. A currently dominant plant substrate is maize silage. However, intensive cultivation of maize (Zea mays L.) as an energy crop in m...

  • Review
  • Open Access
40 Citations
9,429 Views
19 Pages

A wine’s aroma profile is an important part of the criteria affecting wine acceptability by consumers. Its characterisation is complex because volatile molecules usually belong to different classes such as alcohols, esters, aldehydes, acids, te...

  • Article
  • Open Access
31 Citations
6,077 Views
20 Pages

Oenological Characterization of Native Hanseniaspora uvarum Strains

  • Stojan Mančić,
  • Sandra Stamenković Stojanović,
  • Bojana Danilović,
  • Natalija Djordjević,
  • Marko Malićanin,
  • Miodrag Lazić and
  • Ivana Karabegović

The utilization of native yeast strains associated with a distinct terroir for autochthonous grape types represents a novel trend in winemaking, contributing to the production of unique wines with regional character. Hence, this study aimed to isolat...

  • Article
  • Open Access
62 Citations
15,664 Views
11 Pages

Single Cell Protein Production through Multi Food-Waste Substrate Fermentation

  • Alessia Tropea,
  • Antonio Ferracane,
  • Ambrogina Albergamo,
  • Angela Giorgia Potortì,
  • Vincenzo Lo Turco and
  • Giuseppa Di Bella

Today, food valorization represents an important challenge to environmental sustainability. Food waste can be used as a substrate for single cell protein production suitable for animal feed. In this study, animal and agricultural food waste, represen...

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Fermentation - ISSN 2311-5637