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Editor’s Choice Articles

Editor’s Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal.

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12 pages, 1864 KiB  
Article
Genetic Variability of Methane Production and Concentration Measured in the Breath of Polish Holstein-Friesian Cattle
by Mateusz Sypniewski, Tomasz Strabel and Marcin Pszczola
Animals 2021, 11(11), 3175; https://doi.org/10.3390/ani11113175 - 6 Nov 2021
Cited by 14 | Viewed by 3126
Abstract
The genetic architecture of methane (CH4) production remains largely unknown. We aimed to estimate its heritability and to perform genome-wide association studies (GWAS) for the identification of candidate genes associated with two phenotypes: CH4 in parts per million/day (CH4 [...] Read more.
The genetic architecture of methane (CH4) production remains largely unknown. We aimed to estimate its heritability and to perform genome-wide association studies (GWAS) for the identification of candidate genes associated with two phenotypes: CH4 in parts per million/day (CH4 ppm/d) and CH4 in grams/day (CH4 g/d). We studied 483 Polish Holstein-Friesian cows kept on two commercial farms in Poland. Measurements of CH4 and carbon dioxide (CO2) concentrations exhaled by cows during milking were obtained using gas analyzers installed in the automated milking system on the farms. Genomic analyses were performed using a single-step BLUP approach. The percentage of genetic variance explained by SNPs was calculated for each SNP separately and then for the windows of neighbouring SNPs. The heritability of CH4 ppm/d ranged from 0 to 0.14, with an average of 0.085. The heritability of CH4 g/d ranged from 0.13 to 0.26, with an average of 0.22. The GWAS detected potential candidate SNPs on BTA 14 which explained ~0.9% of genetic variance for CH4 ppm/d and ~1% of genetic variance for CH4 g/d. All identified SNPs were located in the TRPS1 gene. We showed that methane traits are partially controlled by genes; however, the detected SNPs explained only a small part of genetic variation—implying that both CH4 ppm/d and CH4 g/d are highly polygenic traits. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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12 pages, 1328 KiB  
Article
Optimal Standardized Ileal Digestible Total Sulfur Amino Acids to Lysine REQUIREMENTS Are Increased in Nursery Pigs Raised under Antibiotic-Free Feeding Regime
by Ping Ren, Ferdinando Almeida, Uislei Orlando, Marcio Gonçalves, Deana Hancock and Mercedes Vazquez-Añón
Animals 2021, 11(11), 3143; https://doi.org/10.3390/ani11113143 - 3 Nov 2021
Cited by 1 | Viewed by 2927
Abstract
This study aimed to investigate the effect of increasing the standardized ileal digestible (SID) total sulfur amino acid to lysine (TSAA:Lys) on the growth performance of nursery pigs raised with or without antibiotics (AGP) and to determine the optimal SID TSAA:Lys in nursery [...] Read more.
This study aimed to investigate the effect of increasing the standardized ileal digestible (SID) total sulfur amino acid to lysine (TSAA:Lys) on the growth performance of nursery pigs raised with or without antibiotics (AGP) and to determine the optimal SID TSAA:Lys in nursery pigs raised without AGP. In Exp. 1, 924 nursery pigs (7.9 ± 1.3 kg), blocked by initial BW and sex, were randomly allotted to one of six treatments, with seven pens per treatment and twenty-two pigs per pen. The treatments were arranged in a 2 × 3 factorial design, with two AGP levels (0 or 50 mg/kg Carbodox) and three levels of SID TSAA:Lys (51.0, 58.5 or 66.0%). In Exp. 2, 990 weaned piglets (5.1 ± 0.9 kg), blocked by initial BW and sex, were randomly allotted to one of five dietary treatments (SID TSAA:Lys at 51, 58, 65, 72 or 79%) in the absence of AGP, with nine pens per treatment and twenty-two pigs per pen. Competing heteroskedastic models including broken-line linear (BLL), broken-line quadratic (BLQ), and quadratic polynomial (QP) were fitted for the growth performance data to estimate the optimal TSAA:Lys. In Exp. 1, AGP supplementation increased (p < 0.05) ADG and ADFI during the 21 d period. Increasing SID TSAA:Lys in the diets with AGP did not affect growth performance; however, increasing SID TSAA:Lys in the diets without AGP resulted in a linear increase (p < 0.05) in ADG and G:F. In Exp. 2, the best-fitting models for ADG and G:F from d 0 to 21 post-weaning were BLL, which yielded the optimal SID TSAA:Lys of 62% and 72%, respectively. The best-fitting models for ADG and G:F from d 21 to 42 post-weaning were BLL, which yielded the optimal SID TSAA:Lys of 59% and 58%, respectively. In conclusion, SID TSAA to Lys requirements under an antibiotic-free feeding regime during the first 21 d post-weaning were 62% and 72% in terms of ADG and G:F, respectively, whereas an SID TSAA:Lys of approximately 58% was required to maximize ADG and G:F for the late nursery phase. Full article
(This article belongs to the Section Animal Nutrition)
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14 pages, 297 KiB  
Review
The Impact of Anthropogenic Climate Change on Egyptian Livestock Production
by Amira A. Goma and Clive J. C. Phillips
Animals 2021, 11(11), 3127; https://doi.org/10.3390/ani11113127 - 1 Nov 2021
Cited by 18 | Viewed by 4220
Abstract
Egypt is one of the hottest countries in the world, and extreme climate events are becoming more frequent, which is consistent with the warming of the planet. The impact of this warming on ecosystems is severe, including on livestock production systems. Under Egyptian [...] Read more.
Egypt is one of the hottest countries in the world, and extreme climate events are becoming more frequent, which is consistent with the warming of the planet. The impact of this warming on ecosystems is severe, including on livestock production systems. Under Egyptian conditions, livestock already suffer heat stress periods in summer. The predicted increases in temperature as result of climate change will affect livestock production by reducing growth and milk production because of appetite suppression and conception rate reductions and will increase animal welfare concerns. In severe cases, these effects can result in death. We review the heat stress effects on livestock behaviour, reproduction, and production in the context of predicted climate change for Egypt over the course of this century and offer alternative scenarios to achieve food security for a growing human population. As an example, we combine predictions for reduced milk production during heat stress and human population trajectories to predict that milk availability per person will decline from 61 kg/year in 2011 to 26 kg/year in 2064. Mitigation strategies are discussed and include the substitution of animal-based foods for plant-based foods and laboratory-grown animal products. Full article
(This article belongs to the Special Issue Environment, Food Production, Health and Welfare Challenges in the Nutrition of Ruminants)
8 pages, 1116 KiB  
Article
Analytical Validation of a New Immunoenzymatic Method for the Measurement of Feline Parathyroid Hormone in Cats with Chronic Kidney Disease
by Jari Zambarbieri, Pierangelo Moretti, Alessia Giordano and Paola Scarpa
Animals 2021, 11(11), 3100; https://doi.org/10.3390/ani11113100 - 29 Oct 2021
Cited by 3 | Viewed by 2611
Abstract
The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. [...] Read more.
The determination of parathyroid hormone (PTH) in cats could be of clinical utility in many metabolic disorders, such as renal diseases, hypercalcemia, or nutritional imbalances. However, the available methods for the measurement of feline PTH are limited, not widely available, and need radioimmunoassays. The aim of this study was to perform the analytical validation of a new immunoenzymatic method for the measurement of feline PTH. Thirty-eight cats affected with chronic kidney disease (CKD) were included. PTH was measured using a two-site immunoenzymatic method validated in humans and dogs (ST AIA-PACK® Intact PTH, Tosoh Bioscience, Tessenderlo, Belgium). The analytical validation provided the evaluation of precision (intra-assay and inter-assay), accuracy (linearity under dilution (LUD) and spike recovery test (SRT)), and the storage stability of serum samples at 20 °C, 4 °C, and −20 °C. The method showed good precision (intra-assay CVs (coefficient of variations) 3.19–9.61%; inter-assay CVs 9.26–15.28%). In both the intra- and inter-assays, the highest imprecision was found with the low concentration pool (9.61% and 15.28%) and accuracy (LUD and SRT r2 = 0.99, p < 0.001), while the stability was optimal up until 7 days at −20 °C (−7.7%). The method was successfully validated in cats, allowing its future use in diagnostic procedures. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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20 pages, 3504 KiB  
Article
Fibropapillomatosis and Chelonid Alphaherpesvirus 5 Infection in Kemp’s Ridley Sea Turtles (Lepidochelys kempii)
by Annie Page-Karjian, Liam Whitmore, Brian A. Stacy, Justin R. Perrault, Jessica A. Farrell, Donna J. Shaver, J. Shelby Walker, Hilary R. Frandsen, Elina Rantonen, Craig A. Harms, Terry M. Norton, Charles Innis, Kelsey Yetsko and David J. Duffy
Animals 2021, 11(11), 3076; https://doi.org/10.3390/ani11113076 - 28 Oct 2021
Cited by 6 | Viewed by 5433
Abstract
Fibropapillomatosis (FP), a debilitating, infectious neoplastic disease, is rarely reported in endangered Kemp’s ridley sea turtles (Lepidochelys kempii). With this study, we describe FP and the associated chelonid alphaherpesvirus 5 (ChHV5) in Kemp’s ridley turtles encountered in the United States during [...] Read more.
Fibropapillomatosis (FP), a debilitating, infectious neoplastic disease, is rarely reported in endangered Kemp’s ridley sea turtles (Lepidochelys kempii). With this study, we describe FP and the associated chelonid alphaherpesvirus 5 (ChHV5) in Kemp’s ridley turtles encountered in the United States during 2006–2020. Analysis of 22 case reports of Kemp’s ridley turtles with FP revealed that while the disease was mild in most cases, 54.5% were adult turtles, a reproductively valuable age class whose survival is a priority for population recovery. Of 51 blood samples from tumor-free turtles and 12 tumor samples from turtles with FP, 7.8% and 91.7%, respectively, tested positive for ChHV5 DNA via quantitative polymerase chain reaction (qPCR). Viral genome shotgun sequencing and phylogenetic analysis of six tumor samples show that ChHV5 sequences in Kemp’s ridley turtles encountered in the Gulf of Mexico and northwestern Atlantic cluster with ChHV5 sequences identified in green (Chelonia mydas) and loggerhead (Caretta caretta) sea turtles from Hawaii, the southwestern Atlantic Ocean, and the Caribbean. Results suggest an interspecific, spatiotemporal spread of FP among Kemp’s ridley turtles in regions where the disease is enzootic. Although FP is currently uncommon in this species, it remains a health concern due to its uncertain pathogenesis and potential relationship with habitat degradation. Full article
(This article belongs to the Special Issue Oncogenic Viruses in Animals)
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16 pages, 307 KiB  
Commentary
Ethical Challenges Posed by Advanced Veterinary Care in Companion Animal Veterinary Practice
by Anne Quain, Michael P. Ward and Siobhan Mullan
Animals 2021, 11(11), 3010; https://doi.org/10.3390/ani11113010 - 20 Oct 2021
Cited by 27 | Viewed by 12123
Abstract
Advanced veterinary care (AVC) of companion animals may yield improved clinical outcomes, improved animal welfare, improved satisfaction of veterinary clients, improved satisfaction of veterinary team members, and increased practice profitability. However, it also raises ethical challenges. Yet, what counts as AVC is difficult [...] Read more.
Advanced veterinary care (AVC) of companion animals may yield improved clinical outcomes, improved animal welfare, improved satisfaction of veterinary clients, improved satisfaction of veterinary team members, and increased practice profitability. However, it also raises ethical challenges. Yet, what counts as AVC is difficult to pinpoint due to continuing advancements. We discuss some of the challenges in defining advanced veterinary care (AVC), particularly in relation to a standard of care (SOC). We then review key ethical challenges associated with AVC that have been identified in the veterinary ethics literature, including poor quality of life, dysthanasia and caregiver burden, financial cost and accessibility of veterinary care, conflicts of interest, and the absence of ethical review for some patients undergoing AVC. We suggest some strategies to address these concerns, including prospective ethical review utilising ethical frameworks and decision-making tools, the setting of humane end points, the role of regulatory bodies in limiting acceptable procedures, and the normalisation of quality-of-life scoring. We also suggest a role for retrospective ethical review in the form of ethics rounds and clinical auditing. Our discussion reenforces the need for a spectrum of veterinary care for companion animals. Full article
(This article belongs to the Special Issue Therapeutics and Advanced Veterinary Care)
17 pages, 809 KiB  
Article
Cyclosporine Treatment in Cats with Presumed Chronic Pancreatitis—A Retrospective Study
by Nina Hoeyrup, Thomas Spillmann and Linda Toresson
Animals 2021, 11(10), 2993; https://doi.org/10.3390/ani11102993 - 19 Oct 2021
Cited by 2 | Viewed by 7042
Abstract
Chronic pancreatitis (CP) is a common disease in middle-aged to older cats. Cyclosporine has been suggested as an alternative treatment when other immunosuppressive treatments are insufficient or contraindicated. However, no published studies have investigated its efficacy on feline CP. The aim of this [...] Read more.
Chronic pancreatitis (CP) is a common disease in middle-aged to older cats. Cyclosporine has been suggested as an alternative treatment when other immunosuppressive treatments are insufficient or contraindicated. However, no published studies have investigated its efficacy on feline CP. The aim of this retrospective study was to evaluate the efficacy of cyclosporine on supranormal serum feline pancreas-specific lipase (Spec fPL) concentrations in cats with presumed CP. Inclusion criteria were history and clinical signs suggestive of CP, serum Spec fPL concentrations above 5.3 μg/L (reference range 0–3.5 μg/L, equivocal range 3.6–5.3 μg/L) on at least two occasions and treatment with cyclosporine for at least three weeks. Serum Spec fPL was analyzed at Idexx Laboratories, Kornwestheim, Germany. Nineteen cats, aged 6.9–17.5 years (median 11.6), were included. No pancreatic biopsies were available. Median (range) serum Spec fPL concentration was 14.2 μg/L (6.1–43.3) at baseline and 6.7 μg/L (0.9–23.6) at follow-up. Cyclosporine treatment (5.0–7.9 mg/kg orally SID) was associated with a significant reduction in serum Spec fPL concentrations (p < 0.001) at follow-up after 23–206 days (median 35). Body weight decreased significantly between inclusion and follow-up (p = 0.013). Significant improvement of clinical signs could not be measured (p = 0.781). This study has several limitations, including unstandardized treatment length and dose, no control group and lack of pancreatic biopsies. Despite the limitations, our results suggest that cyclosporine treatment reduces supranormal serum Spec fPL concentrations in cats with presumed CP. Full article
(This article belongs to the Special Issue Advances in Small Animal Gastroenterology)
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10 pages, 1610 KiB  
Article
Mathematical Models for the Skin to Lumbosacral Epidural Distance in Dogs: A Cadaveric Computed-Tomography Study
by Tsim Christopher Sun, Mara Schier, Michelle Pui Yan Lau and Fernando Martinez-Taboada
Animals 2021, 11(10), 2974; https://doi.org/10.3390/ani11102974 - 15 Oct 2021
Viewed by 2763
Abstract
This study aimed to validate previously published computed tomography (CT) derived mathematical equations with the true skin to lumbosacral epidural distance (SLED) in dog cadavers. Phase 1: The lumbar region of 11 dog cadavers were scanned in sternal recumbency to determine the effect [...] Read more.
This study aimed to validate previously published computed tomography (CT) derived mathematical equations with the true skin to lumbosacral epidural distance (SLED) in dog cadavers. Phase 1: The lumbar region of 11 dog cadavers were scanned in sternal recumbency to determine the effect of cranial, neutral, and caudal pelvic limb positioning on the CT derived lumbosacral epidural distance (CLED). Phase 2: The epidural space was determined using contrast epidurography, and the SLED was analysed against the mathematical equations using a body condition score (BCS) and either the cadaveric occipital-coccygeal length (OCL) (Equation (1): = 7.3 + 0.05*OCL + 16.45*BCS) or the ilium wing distance (IWD) (Equation (2): = 3.5 + 0.56*IWD + 16.6*BCS). There were no differences detected between the pelvic limb positions and the CLED. Both equations demonstrated strong correlations (Equation (1): r = 0.7196; Equation (2): r = 0.7590) with the SLED. The level of agreement was greater for Equation (1) than with Equation (2) (concordance coefficient 0.6061 and 0.3752, respectively). Equation (1) also demonstrated a closer fit to the concordance line compared with Equation (2) (bias correction factor 0.8422 and 0.4960, respectively). Further studies in live anaesthetised dogs will help to determine the usefulness of the pre-procedural knowledge when performing lumbosacral epidurals. Full article
(This article belongs to the Special Issue Loco-Regional Anaesthesia in Veterinary Medicine)
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25 pages, 14567 KiB  
Article
Factors Associated with Fatality in Ontario Thoroughbred Racehorses: 2003–2015
by Peter Physick-Sheard, Amanda Avison and William Sears
Animals 2021, 11(10), 2950; https://doi.org/10.3390/ani11102950 - 13 Oct 2021
Cited by 5 | Viewed by 3243
Abstract
Ontario’s Alcohol and Gaming Commission records equine racing fatalities through its Equine Health Program. The present study examined all Thoroughbred fatalities from 2003 to 2015, inclusive, to identify associations. Official records and details of fatalities were combined in multivariable logistic regression modelling of [...] Read more.
Ontario’s Alcohol and Gaming Commission records equine racing fatalities through its Equine Health Program. The present study examined all Thoroughbred fatalities from 2003 to 2015, inclusive, to identify associations. Official records and details of fatalities were combined in multivariable logistic regression modelling of 236,386 race work-events (433 fatalities), and 459,013 workout work-events (252 fatalities). Fatality rates were 2.94/1000 race starts (all fatalities) and 1.96/1000 (breakdowns only) with an overall rate of 2.61% or 26.1 fatalities/1000 horses. Comparison with published reports reveals rates to be high. Musculoskeletal injury was the predominant complaint and there was a high incidence of horses dying suddenly. Liability was high for young horses early in the season with a differential according to sex and whether a male horse was gelded. Horses undertaking repeated workouts had a higher liability and liability was higher in workouts for horses switching from dirt/synthetic to turf racing and for young horses in sprints. Race distance was not significant but high fatality rates in some large field, distance races combined with effects of age and workload identified groups at particular risk. As field size increased, fatality liability increased for early-finishing horses. Findings suggest jockey strategy could be an important factor influencing fatalities. Probability of fatality declined over the study period. Findings indicate that rapid accumulation of workload in animals early in their preparation is likely to be damaging. Fatality fell toward the end of a season and for horses with a long career history of successful performance; however, horses not exhibiting this robustness and staying power represent the population of greatest concern. Associations may be characterised as representing sources of stress, current or cumulative, and identifying at-risk animals on this basis may be as productive as targeting specific, discrete mechanisms suspected to contribute to individual fatalities. Full article
(This article belongs to the Special Issue The Sustainable Equine)
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11 pages, 3015 KiB  
Article
A Modified Approach for the Ultrasound-Guided Quadratus Lumborum Block in Dogs: A Cadaveric Study
by Jaime Viscasillas, Jose Terrado, Reyes Marti-Scharfhausen, Diego Castiñeiras, Vicente Esteve, Niamh Clancy and Jose Ignacio Redondo
Animals 2021, 11(10), 2945; https://doi.org/10.3390/ani11102945 - 12 Oct 2021
Cited by 21 | Viewed by 5194
Abstract
Ultrasound-guided quadratus lumborum block (QLB) is a locoregional technique described in canine cadavers. The aim of this study was to assess a modified approach to QLB to minimise potential complications such as abdominal organ puncture. Nine canine cadavers were included and were positioned [...] Read more.
Ultrasound-guided quadratus lumborum block (QLB) is a locoregional technique described in canine cadavers. The aim of this study was to assess a modified approach to QLB to minimise potential complications such as abdominal organ puncture. Nine canine cadavers were included and were positioned in lateral recumbency. An ultrasound-guided QLB was performed on each side. The probe was placed in the transverse position over the lumbar muscles just caudal to the last rib, and a needle was advanced in-plane from a dorso-lateral to a ventro-medial. A volume of 0.2 mL kg−1 of a mixture of iomeprol and methylene blue was injected. Computed tomography (CT) and dissection were performed to evaluate the spreading. Success was defined as staining of the nerve with a length of more than 0.6 cm. Potential complications such as intra-abdominal, epidural, or intravascular spreading of the mixture were also assessed. The CT images showed a T13 to L7 vertebra distribution, with a median of 5 (3–6). Dissection showed staining of the nerves from T13 to L4, with a median of 3 (2–5). No complications were found. This modified approach to QLB is safe and shows similar results to the previous studies in canine carcass. Full article
(This article belongs to the Special Issue Loco-Regional Anaesthesia in Veterinary Medicine)
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18 pages, 715 KiB  
Review
Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?
by Teresa Cruz-Bustos, Anna Sophia Feix, Bärbel Ruttkowski and Anja Joachim
Animals 2021, 11(10), 2891; https://doi.org/10.3390/ani11102891 - 4 Oct 2021
Cited by 12 | Viewed by 7214
Abstract
The phylum Apicomplexa is a major group of protozoan parasites including gregarines, coccidia, haemogregarines, haemosporidia and piroplasms, with more than 6000 named species. Three of these subgroups, the coccidia, hemosporidia, and piroplasms, contain parasites that cause important diseases of humans and animals worldwide. [...] Read more.
The phylum Apicomplexa is a major group of protozoan parasites including gregarines, coccidia, haemogregarines, haemosporidia and piroplasms, with more than 6000 named species. Three of these subgroups, the coccidia, hemosporidia, and piroplasms, contain parasites that cause important diseases of humans and animals worldwide. All of them have complex life cycles involving a switch between asexual and sexual reproduction, which is key to their development. Fertilization (i.e., fusion of female and male cells) results in the formation of a zygote that undergoes meiosis, forming a new generation of asexual stages. In eukaryotes, sexual reproduction is the predominant mode of recombination and segregation of DNA. Sex is well documented in many protist groups, and together with meiosis, is frequently linked with transmission to new hosts. Apicomplexan sexual stages constitute a bottleneck in the life cycle of these parasites, as they are obligatory for the development of new transmissible stages. Consequently, the sexual stages represent attractive targets for vaccination. Detailed understanding of apicomplexan sexual biology will pave the way for the design and implementation of effective transmission-blocking strategies for parasite control. This article reviews the current knowledge on the sexual development of Apicomplexa and the progress in transmission-blocking vaccines for their control, their advantages and limitations and outstanding questions for the future. Full article
(This article belongs to the Special Issue Parasites and Parasitic Diseases)
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9 pages, 2425 KiB  
Article
Species-Level Gut Microbiota Analysis after Antibiotic-Induced Dysbiosis in Horses
by Rebecca Di Pietro, Luis G. Arroyo, Mathilde Leclere and Marcio Carvalho Costa
Animals 2021, 11(10), 2859; https://doi.org/10.3390/ani11102859 - 30 Sep 2021
Cited by 10 | Viewed by 4365
Abstract
All current studies have used Illumina short-read sequencing to characterize the equine intestinal microbiota. Long-read sequencing can classify bacteria at the species level. The objectives of this study were to characterize the gut microbiota of horses at the species level before and after [...] Read more.
All current studies have used Illumina short-read sequencing to characterize the equine intestinal microbiota. Long-read sequencing can classify bacteria at the species level. The objectives of this study were to characterize the gut microbiota of horses at the species level before and after trimethoprim sulfadiazine (TMS) administration and to compare results with Illumina sequencing. Nine horses received TMS (30 mg/kg) orally for 5 days twice a day to induce dysbiosis. Illumina sequencing of the V4 region or full-length PacBio sequencing of the 16S rRNA gene was performed in fecal samples collected before and after antibiotic administration. The relative abundance and alpha diversity were compared between the two technologies. PacBio failed to classify the equine intestinal microbiota at the species level but confirmed Bacteroidetes as the most abundant bacteria in the feces of the studied horses, followed by Firmicutes and Fibrobacteres. An unknown species of the Bacteroidales order was highly abundant (13%) and deserves further investigation. In conclusion, PacBio was not suitable to classify the equine microbiota species but detected greater richness and less unclassified bacteria. Further efforts in improving current databanks to be used in equine studies are necessary. Full article
(This article belongs to the Special Issue Equine Microbiota)
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18 pages, 875 KiB  
Review
A Systematic Review of Musculoskeletal Mobilization and Manipulation Techniques Used in Veterinary Medicine
by Kevin K. Haussler, Amie L. Hesbach, Laura Romano, Lesley Goff and Anna Bergh
Animals 2021, 11(10), 2787; https://doi.org/10.3390/ani11102787 - 24 Sep 2021
Cited by 14 | Viewed by 7612
Abstract
Mobilization and manipulation techniques are often used in small animal and equine practice; however, questions remain concerning indications, dosing and efficacy. A bibliographic search was performed to identify peer-reviewed publications from 1980 to 2020 that evaluated the clinical effects of musculoskeletal mobilization and [...] Read more.
Mobilization and manipulation techniques are often used in small animal and equine practice; however, questions remain concerning indications, dosing and efficacy. A bibliographic search was performed to identify peer-reviewed publications from 1980 to 2020 that evaluated the clinical effects of musculoskeletal mobilization and manipulation techniques in dogs, cats and horses. The search strategy identified 883 papers for review. Inclusion and exclusion criteria were applied. The clinical indications, dosages, outcome parameters, and reported efficacy within each publication were recorded and categorized for comparison with scientific quality assessed according to a standardized grading system. Fourteen articles were included in this systematic review of which 13 were equine and one was a canine study. Seven of these were cohort studies and seven were randomized controlled clinical trials. The canine study involved carpal immobilization-remobilization and all equine studies focused on the effects of passive mobilization (n = 5) or manipulation (n = 8) of the axial skeleton. Study quality was low (n = 4), moderate (n = 7), and high (n = 3) and included a wide array of outcome parameters with varying levels of efficacy and duration of therapeutic effects, which prevented further meta-analysis. Therefore, it was difficult to draw firm conclusions despite all studies reporting positive effects. Optimal technique indications and dosages need to be determined to improve the standardization of these treatment options. Full article
(This article belongs to the Collection Veterinary Rehabilitation and Sports Medicine)
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25 pages, 120914 KiB  
Article
Fascioliasis in Llama, Lama glama, in Andean Endemic Areas: Experimental Transmission Capacity by the High Altitude Snail Vector Galba truncatula and Epidemiological Analysis of Its Reservoir Role
by Santiago Mas-Coma, Maria Mercedes Cafrune, Ilra Renata Funatsu, Atilio Jose Mangold, Rene Angles, Paola Buchon, Maria Cecilia Fantozzi, Patricio Artigas, Maria Adela Valero and Maria Dolores Bargues
Animals 2021, 11(9), 2693; https://doi.org/10.3390/ani11092693 - 14 Sep 2021
Cited by 11 | Viewed by 4107
Abstract
South American camelids are definitive hosts of Fasciola hepatica. However, their capacity to participate in the transmission and epidemiology of fascioliasis has never been appropriately studied. Therefore, an F. hepatica isolate from Argentine llama is for the first time analyzed using Galba [...] Read more.
South American camelids are definitive hosts of Fasciola hepatica. However, their capacity to participate in the transmission and epidemiology of fascioliasis has never been appropriately studied. Therefore, an F. hepatica isolate from Argentine llama is for the first time analyzed using Galba truncatula lymnaeids from Bolivia. Experimental follow-up studies included egg embryogenesis, miracidial infection of lymnaeid snails, intramolluscan larval development, cercarial production, chronobiology of cercarial shedding, vector survival to infection, and metacercarial infectivity of mammal host. Shorter prepatent and patent periods were leading to markedly lower cercarial production, shorter cercarial shedding, and a higher negative impact on snail survival. The usually low liver fluke prevalences and intensities and low daily fecal outputs indicate that llamas do not substantially contribute to fascioliasis transmission. The defecating behavior in dung piles far from freshwater collections prevents lymnaeid infection by eggs shed by this camelid. All results suggest the reservoir role of the llama to be negligible and, therefore, no priority within control measures in endemic areas. However, llamas may play a disease-spreading role if used as pack animals in rural areas. In the Northern Bolivian Altiplano human hyperendemic area, neither llamas nor alpacas should be considered for control measures within a One Health action. Full article
(This article belongs to the Special Issue Host-Parasite Relationships in Veterinary Parasitology: Get to Know Your Enemy before Fighting it)
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13 pages, 222 KiB  
Article
Dental Problems and Prophylactic Care in Cats—Knowledge and Perceptions among Swedish Cat Owners and Communication by Veterinary Care Staff
by Kristin Oskarsson, Louise Axelsson Puurtinen and Johanna Christina Penell
Animals 2021, 11(9), 2571; https://doi.org/10.3390/ani11092571 - 31 Aug 2021
Cited by 4 | Viewed by 4842
Abstract
Dental problems are increasingly recognised in cats although many problems are preventable by tooth brushing. However, the knowledge level and preventive measures performed by owners are unclear. Additionally, there is a shortage of information on the communication by veterinary care staff to owners [...] Read more.
Dental problems are increasingly recognised in cats although many problems are preventable by tooth brushing. However, the knowledge level and preventive measures performed by owners are unclear. Additionally, there is a shortage of information on the communication by veterinary care staff to owners on dental health and prophylaxis in cats. The aim was to describe the knowledge and perceptions among Swedish cat owners and the communication by veterinary care staff on dental problems and prevention in cats. We distributed two electronic surveys; to cat owners and care staff, respectively. Of the cat owners, n = 407, 2/3 stated that they have knowledge about dental disease, listing modified eating behaviour as the most common sign followed by gingivitis, halitosis, pain and dental calculus. The main source of information was the internet followed by veterinary care staff, and 47% of the owners reported that they perform preventive oral health measures. The single most frequently stated preventive measure was tooth brushing, which was applied by 28% of the cat owners but with low frequency (daily n = 15, every second day n = 14). Veterinary care staff, n = 179, reported that they inform (47%) or sometimes inform (42%) cat owners on prophylactic dental care, daily or 3–7 times a week (combined 39%) most frequently by oral communication, with tooth brushing as the most common measure recommended. This study suggests that cat owners have relevant knowledge on dental health in cats, but the performed preventive measures are too infrequent to support good oral health in cats. There is room to develop strategies such as improvement in education and communication to increase welfare in cats. This includes consideration of the motivation and practical options for owners of cats who may not tolerate tooth brushing. Full article
(This article belongs to the Special Issue Veterinary Nursing)
19 pages, 4011 KiB  
Article
Effect of Infant Presence on Social Networks of Sterilized and Intact Wild Female Balinese Macaques (Macaca fascicularis)
by Gwennan Giraud, Sebastian Sosa, Alain Hambuckers, Stefan Deleuze, I Nengah Wandia, Marie-Claude Huynen, Pascal Poncin and Fany Brotcorne
Animals 2021, 11(9), 2538; https://doi.org/10.3390/ani11092538 - 29 Aug 2021
Cited by 7 | Viewed by 4354
Abstract
Contraception is increasingly used to control wild animal populations. However, as reproductive condition influences social interactions in primates, the absence of new offspring could influence the females’ social integration. We studied two groups of wild macaques (Macaca fascicularis) including females recently [...] Read more.
Contraception is increasingly used to control wild animal populations. However, as reproductive condition influences social interactions in primates, the absence of new offspring could influence the females’ social integration. We studied two groups of wild macaques (Macaca fascicularis) including females recently sterilized in the Ubud Monkey Forest, Indonesia. We used social network analysis to examine female grooming and proximity networks and investigated the role of infant presence on social centrality and group connectivity, while controlling for the fertility status (sterilized N = 14, intact N = 34). We compared the ego networks of females experiencing different nursing conditions (young infant (YI) vs. old infant (OI) vs. non-nursing (NN) females). YI females were less central in the grooming network than other females while being more central in proximity networks, suggesting they could keep proximity within the group to protect their infant from hazards, while decreasing direct grooming interactions, involving potential risks such as kidnapping. The centrality of sterilized and intact females was similar, except for the proximity network where sterilized females had more partners and a better group connectivity. These results confirm the influence of nursing condition in female macaque social networks and did not show any negative short-term effects of sterilization on social integration. Full article
(This article belongs to the Special Issue Use of Social Network Analysis to Evaluate Animal Welfare)
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17 pages, 628 KiB  
Article
Genetic Parameters for Maternal Performance Traits in Commercially Farmed New Zealand Beef Cattle
by Franziska Weik, Rebecca E. Hickson, Stephen T. Morris, Dorian J. Garrick and Jason A. Archer
Animals 2021, 11(9), 2509; https://doi.org/10.3390/ani11092509 - 26 Aug 2021
Cited by 9 | Viewed by 4357
Abstract
Maternal performance is a major driver of profitability in cow-calf beef cattle enterprises. The aim of this research was to evaluate the inheritance of maternal performance traits and examine the intercorrelation among reproduction, live weight, hip height, body condition and maternal contribution to [...] Read more.
Maternal performance is a major driver of profitability in cow-calf beef cattle enterprises. The aim of this research was to evaluate the inheritance of maternal performance traits and examine the intercorrelation among reproduction, live weight, hip height, body condition and maternal contribution to calf weaning weight in 15-month-old heifers, 2-year-old cows and mature cows in New Zealand beef herds. Data were collected on a total of 14,241 cows and their progeny on five commercial New Zealand hill country farms. Heritabilities were low for reproductive traits in heifers and mature cows (0–0.06) but were greater in 2-year-old cows (0.12–0.21). Body condition scores were lowly (0.15–0.26) and live weights (0.42–0.48) and hip heights (0.47–0.65) highly heritable in heifers, 2-year-old cows and mature cows. Results indicate that 2-year-old cows with higher genetic potential for rebreeding ability may have greater genetic merit for live weight, hip height and body condition as heifers (rg = 0.19–0.54) but are unlikely to be larger cows at maturity (rg = −0.27–−0.10). The maternal genetic effect on weaning weight had a heritability of 0.20 and was negatively genetically correlated with body condition score in lactating cows (rg = −0.55–−0.40) but positively genetically correlated with rebreeding performance (rg = 0.48). Full article
(This article belongs to the Section Animal Genetics and Genomics)
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22 pages, 3178 KiB  
Article
Evolutionary Comparisons of Chelonid Alphaherpesvirus 5 (ChHV5) Genomes from Fibropapillomatosis-Afflicted Green (Chelonia mydas), Olive Ridley (Lepidochelys olivacea) and Kemp’s Ridley (Lepidochelys kempii) Sea Turtles
by Liam Whitmore, Kelsey Yetsko, Jessica A. Farrell, Annie Page-Karjian, Whitney Daniel, Donna J. Shaver, Hilary R. Frandsen, Jennifer Shelby Walker, Whitney Crowder, Caitlin Bovery, Devon Rollinson Ramia, Brooke Burkhalter, Elizabeth Ryan and David J. Duffy
Animals 2021, 11(9), 2489; https://doi.org/10.3390/ani11092489 - 25 Aug 2021
Cited by 10 | Viewed by 4960
Abstract
The spreading global sea turtle fibropapillomatosis (FP) epizootic is threatening some of Earth’s ancient reptiles, adding to the plethora of threats faced by these keystone species. Understanding this neoplastic disease and its likely aetiological pathogen, chelonid alphaherpesvirus 5 (ChHV5), is crucial to understand [...] Read more.
The spreading global sea turtle fibropapillomatosis (FP) epizootic is threatening some of Earth’s ancient reptiles, adding to the plethora of threats faced by these keystone species. Understanding this neoplastic disease and its likely aetiological pathogen, chelonid alphaherpesvirus 5 (ChHV5), is crucial to understand how the disease impacts sea turtle populations and species and the future trajectory of disease incidence. We generated 20 ChHV5 genomes, from three sea turtle species, to better understand the viral variant diversity and gene evolution of this oncogenic virus. We revealed previously underappreciated genetic diversity within this virus (with an average of 2035 single nucleotide polymorphisms (SNPs), 1.54% of the ChHV5 genome) and identified genes under the strongest evolutionary pressure. Furthermore, we investigated the phylogeny of ChHV5 at both genome and gene level, confirming the propensity of the virus to be interspecific, with related variants able to infect multiple sea turtle species. Finally, we revealed unexpected intra-host diversity, with up to 0.15% of the viral genome varying between ChHV5 genomes isolated from different tumours concurrently arising within the same individual. These findings offer important insights into ChHV5 biology and provide genomic resources for this oncogenic virus. Full article
(This article belongs to the Special Issue Oncogenic Viruses in Animals)
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27 pages, 3338 KiB  
Article
DNA Multi-Marker Genotyping and CIAS Morphometric Phenotyping of Fasciola gigantica-Sized Flukes from Ecuador, with an Analysis of the Radix Absence in the New World and the Evolutionary Lymnaeid Snail Vector Filter
by Maria Dolores Bargues, Maria Adela Valero, Gabriel A. Trueba, Marco Fornasini, Angel F. Villavicencio, Rocío Guamán, Alejandra De Elías-Escribano, Ignacio Pérez-Crespo, Patricio Artigas and Santiago Mas-Coma
Animals 2021, 11(9), 2495; https://doi.org/10.3390/ani11092495 - 25 Aug 2021
Cited by 19 | Viewed by 5347
Abstract
Fascioliasis is a disease caused by Fasciola hepatica worldwide transmitted by lymnaeid snails mainly of the Galba/Fossaria group and F. gigantica restricted to parts of Africa and Asia and transmitted by Radix lymnaeids. Concern has recently risen regarding the high pathogenicity [...] Read more.
Fascioliasis is a disease caused by Fasciola hepatica worldwide transmitted by lymnaeid snails mainly of the Galba/Fossaria group and F. gigantica restricted to parts of Africa and Asia and transmitted by Radix lymnaeids. Concern has recently risen regarding the high pathogenicity and human infection capacity of F. gigantica. Abnormally big-sized fasciolids were found infecting sheep in Ecuador, the only South American country where F. gigantica has been reported. Their phenotypic comparison with F. hepatica infecting sheep from Peru, Bolivia and Spain, and F. gigantica from Egypt and Vietnam demonstrated the Ecuadorian fasciolids to have size-linked parameters of F. gigantica. Genotyping of these big-sized fasciolids by rDNA ITS-2 and ITS-1 and mtDNA cox1 and nad1 and their comparison with other countries proved the big-sized fasciolids to belong to F. hepatica. Neither heterozygotic ITS position differentiated the two species, and no introgressed fragments and heteroplasmic positions in mtDNA were found. The haplotype diversity indicates introductions mainly from other South American countries, Europe and North America. Big-sized fasciolids from Ecuador and USA are considered to be consequences of F.gigantica introductions by past livestock importations. The vector specificity filter due to Radix absence should act as driving force in the evolution in such lineages. Full article
(This article belongs to the Special Issue Host-Parasite Relationships in Veterinary Parasitology: Get to Know Your Enemy before Fighting it)
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16 pages, 2370 KiB  
Article
Improved Oral Health and Adaptation to Treatment in Dogs Using Manual or Ultrasonic Toothbrush or Textile of Nylon or Microfiber for Active Dental Home Care
by Lena Olsén, Anna Brissman, Sara Wiman, Fanny Eriksson, Camilla Kaj and Karolina Brunius Enlund
Animals 2021, 11(9), 2481; https://doi.org/10.3390/ani11092481 - 24 Aug 2021
Cited by 7 | Viewed by 10968
Abstract
Poor oral health is a common problem in dogs causing pain and impaired welfare. The gold standard to maintain good oral health and prevent periodontitis is daily tooth brushing. However, many dog owners find it difficult, and dogs often show aversive behavior. There [...] Read more.
Poor oral health is a common problem in dogs causing pain and impaired welfare. The gold standard to maintain good oral health and prevent periodontitis is daily tooth brushing. However, many dog owners find it difficult, and dogs often show aversive behavior. There are several different tools available, and in this intra-subject study, four different active dental home care treatment options were examined: manual and ultrasonic toothbrush, a nylon glove, and a microfiber finger cloth. The treatments were evaluated for efficacy against gingivitis, plaque, and calculus in beagle dogs. Each dog received two treatments on the buccal sides of the teeth once daily, either two types of toothbrushes (N = 10) or two types of textiles (N = 11), on separate sides of the mouth. The dogs’ teeth were assessed before and after the five-week treatment period, with the assessor blinded by additional, similar dogs (N = 7). The study also assessed the dogs’ stress levels during treatment using a fear, anxiety, stress (FAS) protocol. All four treatments improved dog oral health, reducing gingivitis and plaque. Furthermore, the amount of calculus also decreased in some individuals. Adaptation to dental care occurred in the dogs over time. This information can be used to encourage dog owners to brush their dogs’ teeth. Full article
(This article belongs to the Special Issue Veterinary Nursing)
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16 pages, 1471 KiB  
Review
Role of Two-Dimensional Speckle-Tracking Echocardiography in Early Detection of Left Ventricular Dysfunction in Dogs
by Lina Hamabe, Ahmed S. Mandour, Kazumi Shimada, Akiko Uemura, Zeki Yilmaz, Kentaro Nagaoka and Ryou Tanaka
Animals 2021, 11(8), 2361; https://doi.org/10.3390/ani11082361 - 10 Aug 2021
Cited by 16 | Viewed by 5488
Abstract
Two-dimensional speckle-tracking echocardiography (2D–STE) is an advanced echocardiographic technique based on deformation imaging that allows comprehensive evaluation of the myocardial function. Clinical application of 2D–STE holds great potential for its ability to provide valuable information on both global and regional myocardial function and [...] Read more.
Two-dimensional speckle-tracking echocardiography (2D–STE) is an advanced echocardiographic technique based on deformation imaging that allows comprehensive evaluation of the myocardial function. Clinical application of 2D–STE holds great potential for its ability to provide valuable information on both global and regional myocardial function and to quantify cardiac rotation and synchronicity, which are not readily possible with the conventional echocardiography. It has gained growing importance over the past decade, especially in human medicine, and its application includes assessment of myocardial function, detection of subclinical myocardial dysfunction and serving as a prognostic indicator. This review illustrates the fundamental concepts of deformation analysis and gives an overview of the current understanding and its clinical application of this technique in veterinary medicine, with a focus on early detection of left ventricular (LV) dysfunction in dogs. Full article
(This article belongs to the Special Issue Early Detection of Heart Failure in Animals)
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15 pages, 2682 KiB  
Article
Calcinosis in Alpaca Crias (Vicugna pacos) Due to Vitamin D Intoxication—Clinical, Laboratory and Pathological Findings with a Focus on Kidney Function
by Matthias Gerhard Wagener, Carina Helmer, Patricia Kammeyer, Sven Kleinschmidt, Teresa Maria Punsmann, Johanna Maria Meilwes, Cornelia Schwennen, Alexandra von Altrock, Mirja Wilkens, Barbara Schwert, Nicole von Keyserlingk-Eberius and Martin Ganter
Animals 2021, 11(8), 2332; https://doi.org/10.3390/ani11082332 - 7 Aug 2021
Cited by 7 | Viewed by 4472
Abstract
Alpacas kept in Central Europe are often deficient in vitamin D3, which is supplemented orally or by injection by the owners or veterinarians. Vitamin D3 can be specified in two different units (IU and µg), which differ by a factor [...] Read more.
Alpacas kept in Central Europe are often deficient in vitamin D3, which is supplemented orally or by injection by the owners or veterinarians. Vitamin D3 can be specified in two different units (IU and µg), which differ by a factor of 40. By mixing up these units, an overdosage can be induced. In this study, three alpaca crias were examined after vitamin D3 intoxication, with particular reference to kidney function. All three animals developed non-specific clinical alterations 1–2 weeks after a vitamin D3 overdose of approximately 40 times. Plasma of the animals revealed several alterations. The main findings were severe azotemia, hypercalcemia and hyperphosphatemia, 15 days after treatment. Kidney function analysis (endogenous creatinine clearance) in two of the crias revealed severe glomerular damage. All crias died despite intensive treatment within 23 days after vitamin D3 treatment. Necropsy revealed calcification in different organs, mainly the kidneys, lungs and liver. Since nine other crias in the same group were treated with comparable doses of vitamin D3 and no clinical signs were observed in these animals, it is concluded that individual animals show different levels of sensitivity to vitamin D3. Full article
(This article belongs to the Collection Clinical Pathology in Animals)
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10 pages, 1858 KiB  
Article
High Drug Resistance in Feline Mammary Carcinoma Cell Line (FMCm) and Comparison with Human Breast Cancer Cell Line (MCF-7)
by Ana Salomé Correia, Rita Matos, Fátima Gärtner, Irina Amorim and Nuno Vale
Animals 2021, 11(8), 2321; https://doi.org/10.3390/ani11082321 - 6 Aug 2021
Cited by 2 | Viewed by 3916
Abstract
Drug repurposing and drug combination are important therapeutic approaches in cancer therapy. Drug repurposing aims to give new indications to drugs, rather than the original indication, whereas drug combination presupposes that the effect that is obtained should be more beneficial than the effect [...] Read more.
Drug repurposing and drug combination are important therapeutic approaches in cancer therapy. Drug repurposing aims to give new indications to drugs, rather than the original indication, whereas drug combination presupposes that the effect that is obtained should be more beneficial than the effect obtained by the individual drugs. Previously, drug repurposing and the combination of different drugs was evaluated in our research group against human breast cancer cells (MCF-7 cells). Our results demonstrated that the response obtained through the combination of drugs, when compared with the single drugs, led to more synergic responses. Therefore, using potential drugs for repurposing, combined with a reference drug in breast cancer (5-Fluorouracil), was the major aim of this project, but for the first time using the feline mammary carcinoma cell line, FMCm. Surprisingly, the feline neoplastic cells demonstrated considerable resistance to the drugs tested in isolation, and the combination was not effective, which contrasted with the obtained MCF-7 cells’ response. Full article
(This article belongs to the Special Issue Canine and Feline Tumors: Research Advances and Diagnostic Innovations)
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14 pages, 1608 KiB  
Article
Bacterial Contamination of Equine Dentistry Equipment—Effect of Cleaning and Disinfection
by Todd Alsing-Johansson, Anja Pedersen, Karin Bergström, Susanna Sternberg-Lewerin, Johanna Penell and Anna Bergh
Animals 2021, 11(8), 2320; https://doi.org/10.3390/ani11082320 - 5 Aug 2021
Cited by 1 | Viewed by 6024
Abstract
Equine dentistry has developed immensely and human dental equipment, such as handpieces, are often used. Measures to avoid the spread of infectious microorganisms are important, but this is challenging since handpieces are difficult to decontaminate. Thus, it is necessary to develop effective IPC [...] Read more.
Equine dentistry has developed immensely and human dental equipment, such as handpieces, are often used. Measures to avoid the spread of infectious microorganisms are important, but this is challenging since handpieces are difficult to decontaminate. Thus, it is necessary to develop effective IPC measures in equine dentistry. The aim of this study was to contribute to the evidence needed for future evidence-based guidelines on IPC by investigating hygiene in equine dentistry. Used handpieces and dummies (i.e., handpieces not used during dental procedure, reflecting environmental bacterial contamination) and the head support were sampled each day before the first patient, for each patient after treatment, and after decontamination. All equipment was sampled with 3M TM Swab Samplers and the head support additionally sampled with dip slides. After dental procedures, the detected bacterial load was often high on used handpieces, dummies, and the head support. After decontamination, handpieces did not meet the criteria for high-level disinfected equipment. In all but one case decontamination of the head support resulted in a lowered bacterial load. There is a great need for evidence-based guidelines on hygiene in equine dentistry, including IPC measures, to decrease the risk of spreading infectious microorganisms between patients, facilities, and stables. Full article
(This article belongs to the Special Issue Veterinary Nursing)
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27 pages, 423 KiB  
Review
Adaptation of Livestock to New Diets Using Feed Components without Competition with Human Edible Protein Sources—A Review of the Possibilities and Recommendations
by Marinus F. W. te Pas, Teun Veldkamp, Yvette de Haas, André Bannink and Esther D. Ellen
Animals 2021, 11(8), 2293; https://doi.org/10.3390/ani11082293 - 3 Aug 2021
Cited by 18 | Viewed by 5789
Abstract
Livestock feed encompasses both human edible and human inedible components. Human edible feed components may become less available for livestock. Especially for proteins, this calls for action. This review focuses on using alternative protein sources in feed and protein efficiency, the expected problems, [...] Read more.
Livestock feed encompasses both human edible and human inedible components. Human edible feed components may become less available for livestock. Especially for proteins, this calls for action. This review focuses on using alternative protein sources in feed and protein efficiency, the expected problems, and how these problems could be solved. Breeding for higher protein efficiency leading to less use of the protein sources may be one strategy. Replacing (part of) the human edible feed components with human inedible components may be another strategy, which could be combined with breeding for livestock that can efficiently digest novel protein feed sources. The potential use of novel protein sources is discussed. We discuss the present knowledge on novel protein sources, including the consequences for animal performance and production costs, and make recommendations for the use and optimization of novel protein sources (1) to improve our knowledge on the inclusion of human inedible protein into the diet of livestock, (2) because cooperation between animal breeders and nutritionists is needed to share knowledge and combine expertise, and (3) to investigate the effect of animal-specific digestibility of protein sources for selective breeding for each protein source and for precision feeding. Nutrigenetics and nutrigenomics will be important tools. Full article
(This article belongs to the Section Animal Nutrition)
14 pages, 304 KiB  
Review
Current Status on Canine Foetal Fluid and Adnexa Derived Mesenchymal Stem Cells
by Eleonora Iacono, Romina Marcoccia and Barbara Merlo
Animals 2021, 11(8), 2254; https://doi.org/10.3390/ani11082254 - 30 Jul 2021
Cited by 2 | Viewed by 3250
Abstract
Effective standards of care treatment guidelines have been developed for many canine diseases. However, a subpopulation of patients is partially or completely refractory to these protocols, so their owners seek novel therapies such as treatments with MSCs. Although in dogs, as with human [...] Read more.
Effective standards of care treatment guidelines have been developed for many canine diseases. However, a subpopulation of patients is partially or completely refractory to these protocols, so their owners seek novel therapies such as treatments with MSCs. Although in dogs, as with human medicine, the most studied MSCs sources have been bone marrow and adipose tissue, in recent years, many researchers have drawn attention towards alternative sources, such as foetal adnexa and fluid, since they possess many advantages over bone marrow and adipose tissue. Foetal adnexa and fluid could be considered as discarded material; therefore, sampling is non-invasive, inexpensive and free from ethical considerations. Furthermore, MSCs derived from foetal adnexa and fluid preserve some of the characteristics of the primitive embryonic layers from which they originate and seem to present immune-modulatory properties that make them a good candidate for allo- and xenotransplantation. The aim of the present review is to offer an update on the state of the art on canine MSCs derived from foetal adnexa and fluid focusing on the findings in their clinical setting. Full article
(This article belongs to the Collection Stem Cells in Domestic Animals: Applications in Health and Production)
22 pages, 1221 KiB  
Review
Etiology of Colitis-Complex Diarrhea in Growing Pigs: A Review
by Farhad M. Panah, Charlotte Lauridsen, Ole Højberg and Tina Skau Nielsen
Animals 2021, 11(7), 2151; https://doi.org/10.3390/ani11072151 - 20 Jul 2021
Cited by 15 | Viewed by 6612
Abstract
Colitis-complex diarrhea (CCD) in pigs can be defined as a type of diarrhea, which is associated with colonic inflammation and disrupted colonic gut barrier functionality in growing pigs (4–16 weeks post-weaning). It is a challenge for the pig industry as it is associated [...] Read more.
Colitis-complex diarrhea (CCD) in pigs can be defined as a type of diarrhea, which is associated with colonic inflammation and disrupted colonic gut barrier functionality in growing pigs (4–16 weeks post-weaning). It is a challenge for the pig industry as it is associated with the high use of antibiotics, reduced animal welfare, and depressed growth rate. The exact etiology of CCD is still unclear; however, pathogens including Brachyspira (B.) hyodysenteriae, B. pilosicoli, and swine whipworms such as Trichuris (T.) suis have been involved in specific colitis (SC). In the absence of specific pathogens, dietary factors, such as high levels of protein, pelleted feedstuffs, and lack of sufficient antioxidants, can result in non-specific colitis (NSC). On the other hand, supplement of polyunsaturated fatty acids (PUFA) and polyphenols, sufficient supply of essential amino acids (e.g., threonine, cysteine, and proline), short-chain fatty acids (SCFA; especially butyrate), and resistant starch have shown to confer preventing/ameliorating effects on CCD. Different putative biomarkers associated with CCD have been presented. It is anticipated that a comprehensive picture of the possible causes of CCD and potential dietary interventions could cast light on the direction of future studies aimed at developing preventive and curative strategies against CCD in growing pigs. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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18 pages, 7301 KiB  
Article
First Data on the Helminth Community of the Smallest Living Mammal on Earth, the Etruscan Pygmy Shrew, Suncus etruscus (Savi, 1822) (Eulipotyphla: Soricidae)
by María Teresa Galán-Puchades, Santiago Mas-Coma, María Adela Valero and Màrius V. Fuentes
Animals 2021, 11(7), 2074; https://doi.org/10.3390/ani11072074 - 12 Jul 2021
Cited by 5 | Viewed by 6691
Abstract
Suncus etruscus is the smallest living mammal on Earth by mass. Most adults weigh 1.8–3 g with a body length of 35–48 mm. Catching it in small mammal traps in nature is extremely difficult due to its minute size, and therefore special trapping [...] Read more.
Suncus etruscus is the smallest living mammal on Earth by mass. Most adults weigh 1.8–3 g with a body length of 35–48 mm. Catching it in small mammal traps in nature is extremely difficult due to its minute size, and therefore special trapping methods must be used. We had the unique opportunity of studying, for the first time, the helminth parasites of 166 individuals of S. etruscus, part of the largest collection in the world, which belonged to the French scientist Dr Roger Fons (1942–2016). A total of 150 individuals were captured in the Banyuls-Cerbère area (France) and 16 in the island of Corsica (France). We found seven helminth species, specifically, the cestodes Joyeuxiella pasqualei larvae, Mesocestoides sp. larvae, Staphylocystis claudevaucheri, S. banyulsensis, S. cerberensis, and Pseudhymenolepis sp., and the nematodes Aonchotheca sp. and Nematoda gen. sp. larvae. Neither trematodes nor acanthocephalans were detected. We provide prevalences, infracommunity compositions, and helminth associations. The adult helminth community of S. etruscus seems to be highly specific, i.e., oioxenous, and linked to its insectivore diet. Due to its small size, S. etruscus has undergone numerous physiological adaptations that have probably influenced its helminth spectrum as well as its helminth specificity. Full article
(This article belongs to the Special Issue Helminths of Small Mammals)
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14 pages, 2704 KiB  
Article
Effects of Allogeneic Mesenchymal Stem Cell Transplantation in Dogs with Inflammatory Bowel Disease Treated with and without Corticosteroids
by José Ignacio Cristóbal, Francisco Javier Duque, Jesús María Usón-Casaús, Patricia Ruiz, Esther López Nieto and Eva María Pérez-Merino
Animals 2021, 11(7), 2061; https://doi.org/10.3390/ani11072061 - 10 Jul 2021
Cited by 16 | Viewed by 5235
Abstract
Mesenchymal stem cells have proven to be a promising alternative to conventional steroids to treat canine inflammatory bowel disease (IBD). However, their administration requires a washout period of immunosuppressive drugs that can lead to an exacerbation of the symptoms. Therefore, the feasibility and [...] Read more.
Mesenchymal stem cells have proven to be a promising alternative to conventional steroids to treat canine inflammatory bowel disease (IBD). However, their administration requires a washout period of immunosuppressive drugs that can lead to an exacerbation of the symptoms. Therefore, the feasibility and effects of the combined application of stem cells and prednisone in IBD-dogs without adequate response to corticosteroids was evaluated for the first time in this study over a long- term follow up. Two groups of dogs with IBD, one without treatment and another with prednisone treatment, received a single infusion of stem cells. The clinical indices, albumin and cobalamin were determined prior to the infusion and after one, three, six and 12 months. In both groups, all parameters significantly improved at each time point. In parallel, the steroid dosage was gradually reduced until it was suppressed in all patients a year after the cell therapy. Therefore, cell therapy can significantly and safely improve the disease condition in dogs with IBD receiving or not receiving prednisone. Furthermore, the steroid dosage can be significantly reduced or cancelled after the stem cell infusion. Their beneficial effects are stable over time and are long lasting. Full article
(This article belongs to the Special Issue Advances in Small Animal Gastroenterology)
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15 pages, 2345 KiB  
Article
GPS Coordinates for Modelling Correlated Herd Effects in Genomic Prediction Models Applied to Hanwoo Beef Cattle
by Beatriz Castro Dias Cuyabano, Gabriel Rovere, Dajeong Lim, Tae Hun Kim, Hak Kyo Lee, Seung Hwan Lee and Cedric Gondro
Animals 2021, 11(7), 2050; https://doi.org/10.3390/ani11072050 - 9 Jul 2021
Cited by 4 | Viewed by 4492
Abstract
It is widely known that the environment influences phenotypic expression and that its effects must be accounted for in genetic evaluation programs. The most used method to account for environmental effects is to add herd and contemporary group to the model. Although generally [...] Read more.
It is widely known that the environment influences phenotypic expression and that its effects must be accounted for in genetic evaluation programs. The most used method to account for environmental effects is to add herd and contemporary group to the model. Although generally informative, the herd effect treats different farms as independent units. However, if two farms are located physically close to each other, they potentially share correlated environmental factors. We introduce a method to model herd effects that uses the physical distances between farms based on the Global Positioning System (GPS) coordinates as a proxy for the correlation matrix of these effects that aims to account for similarities and differences between farms due to environmental factors. A population of Hanwoo Korean cattle was used to evaluate the impact of modelling herd effects as correlated, in comparison to assuming the farms as completely independent units, on the variance components and genomic prediction. The main result was an increase in the reliabilities of the predicted genomic breeding values compared to reliabilities obtained with traditional models (across four traits evaluated, reliabilities of prediction presented increases that ranged from 0.05 ± 0.01 to 0.33 ± 0.03), suggesting that these models may overestimate heritabilities. Although little to no significant gain was obtained in phenotypic prediction, the increased reliability of the predicted genomic breeding values is of practical relevance for genetic evaluation programs. Full article
(This article belongs to the Collection Advances in Cattle Breeding, Genetics and Genomics)
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17 pages, 706 KiB  
Review
An Overview of the Use of Genotyping Techniques for Assessing Genetic Diversity in Local Farm Animal Breeds
by Anna Olschewsky and Dirk Hinrichs
Animals 2021, 11(7), 2016; https://doi.org/10.3390/ani11072016 - 6 Jul 2021
Cited by 27 | Viewed by 6256
Abstract
Globally, many local farm animal breeds are threatened with extinction. However, these breeds contribute to the high amount of genetic diversity required to combat unforeseen future challenges of livestock production systems. To assess genetic diversity, various genotyping techniques have been developed. Based on [...] Read more.
Globally, many local farm animal breeds are threatened with extinction. However, these breeds contribute to the high amount of genetic diversity required to combat unforeseen future challenges of livestock production systems. To assess genetic diversity, various genotyping techniques have been developed. Based on the respective genomic information, different parameters, e.g., heterozygosity, allele frequencies and inbreeding coefficient, can be measured in order to reveal genetic diversity between and within breeds. The aim of the present work was to shed light on the use of genotyping techniques in the field of local farm animal breeds. Therefore, a total of 133 studies across the world that examined genetic diversity in local cattle, sheep, goat, chicken and pig breeds were reviewed. The results show that diversity of cattle was most often investigated with microsatellite use as the main technique. Furthermore, a large variety of diversity parameters that were calculated with different programs were identified. For 15% of the included studies, the used genotypes are publicly available, and, in 6%, phenotypes were recorded. In conclusion, the present results provide a comprehensive overview of the application of genotyping techniques in the field of local breeds. This can provide helpful insights to advance the conservation of breeds. Full article
(This article belongs to the Special Issue Genomics Applied to Conservation of Farm Animal Genetic Diversity)
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28 pages, 1647 KiB  
Article
Effectiveness of Two Plant-Based In-Feed Additives against an Escherichia coli F4 Oral Challenge in Weaned Piglets
by Daniel Montoya, Matilde D’Angelo, Susana M. Martín-Orúe, Agustina Rodríguez-Sorrento, Mireia Saladrigas-García, Coralie Araujo, Thibaut Chabrillat, Sylvain Kerros and Lorena Castillejos
Animals 2021, 11(7), 2024; https://doi.org/10.3390/ani11072024 - 6 Jul 2021
Cited by 9 | Viewed by 6477
Abstract
This study evaluates the efficacy of two plant-based feed supplementations to fight colibacillosis in weanlings. A total of 96 piglets (32 pens) were assigned to four diets: a control diet (T1) or supplemented with ZnO (2500 ppm Zn) (T2) or two different plant [...] Read more.
This study evaluates the efficacy of two plant-based feed supplementations to fight colibacillosis in weanlings. A total of 96 piglets (32 pens) were assigned to four diets: a control diet (T1) or supplemented with ZnO (2500 ppm Zn) (T2) or two different plant supplements, T3 (1 kg/t; based on essential oils) and T4 (T3 + 1.5 kg/t based on non-volatile compounds). After one week, animals were challenged with ETEC F4, and 8 days after, one animal per pen was euthanized. Performance, clinical signs, microbial analysis, inflammatory response, intestinal morphology, and ileal gene expression were assessed. ZnO improved daily gains 4 days after challenge, T3 and T4 showing intermediate values (96, 249, 170, and 157 g/d for T1, T2, T3, and T4, p = 0.035). Fecal lactobacilli were higher with T3 and T4 compared to ZnO (7.55, 6.26, 8.71, and 8.27 cfu/gFM; p = 0.0007) and T3 increased the lactobacilli/coliforms ratio (p = 0.002). T4 was associated with lower levels of Pig-MAP (p = 0.07) and increases in villus/crypt ratio (1.49, 1.90, 1.73, and 1.84; p = 0.009). Moreover, T4 was associated with an upregulation of the REG3G gene (p = 0.013; pFDR = 0.228) involved in the immune response induced by enteric pathogens. In conclusion, both plant supplements enhanced animal response in front of an ETEC F4 challenge probably based on different modes of action. Full article
(This article belongs to the Special Issue Plant-Based Alternatives to Antimicrobial in Animal Feed)
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18 pages, 944 KiB  
Article
Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique
by Matthias Schilde, Dirk von Soosten, Liane Hüther, Susanne Kersten, Ulrich Meyer, Annette Zeyner and Sven Dänicke
Animals 2021, 11(6), 1784; https://doi.org/10.3390/ani11061784 - 15 Jun 2021
Cited by 9 | Viewed by 5514
Abstract
Methane (CH4) from ruminal feed degradation is a major pollutant from ruminant livestock, which calls for mitigation strategies. The purpose of the present 4 × 2 factorial arrangement was to investigate the dose–response relationships between four doses of the CH4 [...] Read more.
Methane (CH4) from ruminal feed degradation is a major pollutant from ruminant livestock, which calls for mitigation strategies. The purpose of the present 4 × 2 factorial arrangement was to investigate the dose–response relationships between four doses of the CH4 inhibitor 3-nitrooxypropanol (3-NOP) and potential synergistic effects with low (LC) or high (HC) concentrate feed proportions (CFP) on CH4 reduction as both mitigation approaches differ in their mode of action (direct 3-NOP vs. indirect CFP effects). Diet substrates and 3-NOP were incubated in a rumen simulation technique to measure the concentration and production of volatile fatty acids (VFA), fermentation gases as well as substrate disappearance. Negative side effects on fermentation regarding total VFA and gas production as well as nutrient degradability were observed for neither CFP nor 3-NOP. CH4 production decreased from 10% up to 97% in a dose-dependent manner with increasing 3-NOP inclusion rate (dose: p < 0.001) but irrespective of CFP (CFP × dose: p = 0.094). Hydrogen gas accumulated correspondingly with increased 3-NOP dose (dose: p < 0.001). In vitro pH (p = 0.019) and redox potential (p = 0.066) varied by CFP, whereas the latter fluctuated with 3-NOP dose (p = 0.01). Acetate and iso-butyrate (mol %) decreased with 3-NOP dose, whereas iso-valerate increased (dose: p < 0.001). Propionate and valerate varied inconsistently due to 3-NOP supplementation. The feed additive 3-NOP was proven to be a dose-dependent yet effective CH4 inhibitor under conditions in vitro. The observed lack of additivity of increased CFP on the CH4 inhibition potential of 3-NOP needs to be verified in future research testing further diet types both in vitro and in vivo. Full article
(This article belongs to the Section Animal Nutrition)
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18 pages, 1411 KiB  
Article
Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets
by Matteo Dell’Anno, Serena Reggi, Valentina Caprarulo, Monika Hejna, Carlo Angelo Sgoifo Rossi, Maria Luisa Callegari, Antonella Baldi and Luciana Rossi
Animals 2021, 11(6), 1693; https://doi.org/10.3390/ani11061693 - 6 Jun 2021
Cited by 22 | Viewed by 6419
Abstract
The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). [...] Read more.
The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (p < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased Prevotella and Fibrobacter genera presence (p < 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (p < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota. Full article
(This article belongs to the Special Issue Plant-Based Alternatives to Antimicrobial in Animal Feed)
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20 pages, 7309 KiB  
Article
Bidimensional and Contrast-Enhanced Ultrasonography of the Spleen in Dogs Affected by Leishmaniosis
by Massimo De Majo, Giulia Donato, Marisa Masucci, Cyndi Mangano, Maria Flaminia Persichetti, Luigi Liotta, Giuseppe Mazzullo, Rosanna Visalli, Marco Quartuccio, Nicola Maria Iannelli, Santo Cristarella and Maria Grazia Pennisi
Animals 2021, 11(5), 1437; https://doi.org/10.3390/ani11051437 - 17 May 2021
Cited by 2 | Viewed by 4555
Abstract
Canine leishmaniosis (CanL) is responsible for splenic pathological changes. The main features detectable from ultrasound examination are splenomegaly and diffuse alterations of the echostructure. The study aimed to highlight whether these ultrasound changes are related to the severity of the disease or to [...] Read more.
Canine leishmaniosis (CanL) is responsible for splenic pathological changes. The main features detectable from ultrasound examination are splenomegaly and diffuse alterations of the echostructure. The study aimed to highlight whether these ultrasound changes are related to the severity of the disease or to a modification of splenic microvascularization that can be detected in vivo through contrast-enhanced ultrasonography (CEUS). Twenty-five adult dogs tested for CanL were enrolled in this prospective, controlled study and staged according to LeishVet guidelines. Bidimensional ultrasonography revealed that splenomegaly was seen in 50% of the affected dogs, and diffuse parenchymal changes were seen in more than 60% of dogs with splenomegaly, showing a positive correlation with severity of the disease; therefore, splenomegaly could be of prognostic significance. CEUS showed that a persistent heterogeneous distribution pattern appeared only in spleens with diffuse echostructure alterations. The evaluation of quantitative CEUS parameters regarding the volume and velocity of flow in three regions of interest did not show differences between affected and control dogs. Diffuse spleen microvascular modifications evidenced by CEUS were reported for the first time in dogs with CanL. In endemic areas, CanL could be included in the differential diagnoses list when detecting splenic alterations in dogs. Full article
(This article belongs to the Special Issue Contrast-Enhanced Ultrasound (CEUS) in Small and Large Animals)
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17 pages, 7868 KiB  
Article
Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart
by Alexandros Hardas, Alejandro Suárez-Bonnet, Sam Beck, William E. Becker, Gustavo A. Ramírez and Simon L. Priestnall
Animals 2021, 11(5), 1409; https://doi.org/10.3390/ani11051409 - 14 May 2021
Cited by 5 | Viewed by 5492
Abstract
Canine gastric carcinoma (CGC) affects both sexes in relatively equal proportions, with a mean age of nine years, and the highest frequency in Staffordshire bull terriers. The most common histological subtype in 149 CGC cases was the undifferentiated carcinoma. CGCs were associated with [...] Read more.
Canine gastric carcinoma (CGC) affects both sexes in relatively equal proportions, with a mean age of nine years, and the highest frequency in Staffordshire bull terriers. The most common histological subtype in 149 CGC cases was the undifferentiated carcinoma. CGCs were associated with increased chronic inflammation parameters and a greater chronic inflammatory score when Helicobacter spp. were present. Understanding the molecular pathways of gastric carcinoma is challenging. All markers showed variable expression for each subtype. Expression of the cell cycle regulator 14-3-3σ was positive in undifferentiated, tubular and papillary carcinomas. This demonstrates that 14-3-3σ could serve as an immunohistochemical marker in routine diagnosis and that mucinous, papillary and signet-ring cell (SRC) carcinomas follow a 14-3-3σ independent pathway. p16, another cell cycle regulator, showed increased expression in mucinous and SRC carcinomas. Expression of the adhesion molecules E-cadherin and CD44 appear context-dependent, with switching within tumor emboli potentially playing an important role in tumor cell survival, during invasion and metastasis. Within neoplastic emboli, acinar structures lacked expression of all markers, suggesting an independent molecular pathway that requires further investigation. These findings demonstrate similarities and differences between dogs and humans, albeit further clinicopathological data and molecular analysis are required. Full article
(This article belongs to the Special Issue Comparative Pathology and Immunohistochemistry of Veterinary Species)
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13 pages, 1284 KiB  
Article
Bovine Intelectin 2 Expression as a Biomarker of Paratuberculosis Disease Progression
by Cristina Blanco Vázquez, Ana Balseiro, Marta Alonso-Hearn, Ramón A. Juste, Natalia Iglesias, Maria Canive and Rosa Casais
Animals 2021, 11(5), 1370; https://doi.org/10.3390/ani11051370 - 12 May 2021
Cited by 8 | Viewed by 3092
Abstract
Paratuberculosis (PTB), a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is responsible for important economic losses in the dairy industry. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) [...] Read more.
Paratuberculosis (PTB), a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is responsible for important economic losses in the dairy industry. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) samples of animals with focal (log2 fold-change = 10.6) and diffuse (log2 fold-change = 6.8) PTB-associated lesions compared to animals without lesions. This study analyzes the potential use of ITLN2, a protein that has been described as fundamental in the innate immune response to infections, as a biomarker of MAP infection. The presence of ITLN2 was investigated by quantitative immunohistochemical analysis of ICV samples of 20 Holstein Friesian cows showing focal (n = 5), multifocal (n = 5), diffuse (n = 5) and no histological lesions (n = 5). Significant differences were observed in the mean number of ITLN2 immunostained goblet and Paneth cells between the three histopathological types and the control. The number of immunolabelled cells was higher in the focal histopathological type (116.9 ± 113.9) followed by the multifocal (108.7 ± 140.5), diffuse (76.5 ± 97.8) and control types (41.0 ± 81.3). These results validate ITLN2 as a post-mortem biomarker of disease progression. Full article
(This article belongs to the Special Issue Paratuberculosis in Livestock: Innovative Approaches for Early Diagnosis and Disease Control)
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17 pages, 4803 KiB  
Article
Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase
by Maria Morini, Angelo Peli, Riccardo Rinnovati, Giuseppe Magazzù, Noemi Romagnoli, Alessandro Spadari and Marco Pietra
Animals 2021, 11(5), 1376; https://doi.org/10.3390/ani11051376 - 12 May 2021
Cited by 2 | Viewed by 3445
Abstract
Severe equine asthma (EA) syndrome is a chronic obstructive disease characterized by exaggerated contraction, inflammation, and structural alteration of the airways in adult horses, when exposed to airborne molds and particulate material. However, little is known about the relationship between the degree and [...] Read more.
Severe equine asthma (EA) syndrome is a chronic obstructive disease characterized by exaggerated contraction, inflammation, and structural alteration of the airways in adult horses, when exposed to airborne molds and particulate material. However, little is known about the relationship between the degree and type of inflammation on one hand, and the severity of the disease and the response to treatment on the other. Furthermore, to date, very few studies evaluate the diagnostic value of histology and immunohistochemical features of endoscopic biopsies on subjects with severe equine asthma. To investigate the expression of two inflammatory markers (NKA and IL-8) before, during, and after the exacerbation of severe EA, a histological and immunohistochemical study was carried out on a series of biopsy samples collected by bronchoscopy from six EA-affected horses subjected to process exacerbation through environmental stimuli and then to pharmacological treatment. The application of a histological biopsy scoring system revealed a significant difference between control cases and the EA-affected horses in all experimental phases (asymptomatic, early exacerbation phase, late exacerbation phase, and remission phase). For immunohistochemistry (IHC), only the intensity of NKA positivity increases significantly between control horses and the EA horses at late exacerbation and remission phases. In EA-affected horses, a difference was detected by comparing histology between asymptomatic and remission phase, meanwhile, NKA and IL-8 showed no differences between the experimental phases. Based on these results we can assert that: (1) The endoscopic biopsies generate reliable and homogeneous samples in the entire bronchial tree; (2) the clinical improvement associated with treatment is characterized by a significant worsening of the histological findings; and (3) the NKA immunopositivity seems to increase significantly rather than decrease, as one would have expected, after pharmacological treatment. Further studies are necessary both to implement the number of samples and to use other markers of inflammation to characterize the potential role of cytokines in the diagnosis and therapeutic approach of severe equine asthma. Full article
(This article belongs to the Special Issue Comparative Pathology and Immunohistochemistry of Veterinary Species)
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8 pages, 1646 KiB  
Article
Maneuverability of the Scope and Instruments within Three Different Single-Incision Laparoscopic Ports: An Experimental Pilot Study
by Georg Haider, Ursula Schulz, Nikola Katic, Christian Peham and Gilles Dupré
Animals 2021, 11(5), 1242; https://doi.org/10.3390/ani11051242 - 26 Apr 2021
Cited by 1 | Viewed by 2627
Abstract
Single-port access systems (SPASs) are currently used in human and veterinary surgeries. However, they pose technical challenges, such as instrument crowding, intra- and extracorporeal instrument collision, and reduced maneuverability. Studies comparing the maneuverability of the scopes and instruments in different SPASs are lacking. [...] Read more.
Single-port access systems (SPASs) are currently used in human and veterinary surgeries. However, they pose technical challenges, such as instrument crowding, intra- and extracorporeal instrument collision, and reduced maneuverability. Studies comparing the maneuverability of the scopes and instruments in different SPASs are lacking. This study aimed to compare the maneuverability of three different SPASs: the Covidien SILS-port, Storz Endocone, and glove port. A clear acrylic box with artificial skin placed at the bottom was used to mimic the abdominal wall and cavity. The three SPASs were placed from below, and a 10-mm endoscope and 5-mm instrument were introduced. A motion analysis system consisting of 18 cameras and motion analysis software were used to track the movement of the endoscope and instrument, to determine the volume of the cone-shaped, three-dimensional figures over which movement was possible, with higher values indicating greater maneuverability. The Mann–Whitney U test was used for the analysis. The maneuverability of the endoscope alone was significantly higher in the glove port system than in the other two SPASs. When inserting an additional instrument, the maneuverability significantly decreased in the SILS-port and Endocone, but not in the glove port. The highest maneuverability overall was found in the glove port. Full article
(This article belongs to the Special Issue Veterinary Minimally Invasive Surgery)
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13 pages, 608 KiB  
Article
Economic Feasibility of Mixed-Species Grazing to Improve Rangeland Productivity
by Kayla Hintze, Courtney Bir and Derrell Peel
Animals 2021, 11(5), 1226; https://doi.org/10.3390/ani11051226 - 23 Apr 2021
Cited by 4 | Viewed by 3710
Abstract
Pasture and grazing land in the southern and central Great Plains is being invaded by woody species, especially eastern redcedar. As a result of woody plant encroachment, cattle production on native rangeland is becoming less profitable because stocking rates must be decreased. Eastern [...] Read more.
Pasture and grazing land in the southern and central Great Plains is being invaded by woody species, especially eastern redcedar. As a result of woody plant encroachment, cattle production on native rangeland is becoming less profitable because stocking rates must be decreased. Eastern redcedar encroachment can be controlled by grazing management, herbicide use, prescribed fire, mechanical control and mixed species grazing. This study utilizes traditional management practices, prescribed fire and three types of mixed species grazing operations to determine the most economically feasible way to manage redcedar encroachment on rangeland. The cost-benefit analysis in this study found that the source of redcedar management on rangeland with the highest net present value was the use of a breeding goat operation in which goats were grazed alongside cattle with the use of prescribed fire. This suggests that producers who are fighting redcedar encroachment will likely be able to implement a mixed species grazing operation with breeding goats to better manage their land and increase returns. Full article
(This article belongs to the Special Issue Data-Driven Decision Making in Animal Industries)
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21 pages, 4279 KiB  
Review
Challenges and Tendencies of Automatic Milking Systems (AMS): A 20-Years Systematic Review of Literature and Patents
by Alessia Cogato, Marta Brščić, Hao Guo, Francesco Marinello and Andrea Pezzuolo
Animals 2021, 11(2), 356; https://doi.org/10.3390/ani11020356 - 31 Jan 2021
Cited by 46 | Viewed by 7171
Abstract
Over the last two decades, the dairy industry has adopted the use of Automatic Milking Systems (AMS). AMS have the potential to increase the effectiveness of the milking process and sustain animal welfare. This study assessed the state of the art of research [...] Read more.
Over the last two decades, the dairy industry has adopted the use of Automatic Milking Systems (AMS). AMS have the potential to increase the effectiveness of the milking process and sustain animal welfare. This study assessed the state of the art of research activities on AMS through a systematic review of scientific and industrial research. The papers and patents of the last 20 years (2000–2019) were analysed to assess the research tendencies. The words appearing in title, abstract and keywords of a total of 802 documents were processed with the text mining tool. Four clusters were identified (Components, Technology, Process and Animal). For each cluster, the words frequency analysis enabled us to identify the research tendencies and gaps. The results showed that focuses of the scientific and industrial research areas complementary, with scientific papers mainly dealing with topics related to animal and process, and patents giving priority to technology and components. Both scientific and industrial research converged on some crucial objectives, such as animal welfare, process sustainability and technological development. Despite the increasing interest in animal welfare, this review highlighted that further progress is needed to meet the consumers’ demand. Moreover, milk yield is still regarded as more valuable compared to milk quality. Therefore, additional effort is necessary on the latter. At the process level, some gaps have been found related to cleaning operations, necessary to improve milk quality and animal health. The use of farm data and their incorporation on herd decision support systems (DSS) appeared optimal. The results presented in this review may be used as an overall assessment useful to address future research. Full article
(This article belongs to the Special Issue Innovations in Livestock Farms)
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27 pages, 3344 KiB  
Review
A Review on Mitigating Fear and Aggression in Dogs and Cats in a Veterinary Setting
by Stefanie Riemer, Carmen Heritier, Ines Windschnurer, Lydia Pratsch, Christine Arhant and Nadja Affenzeller
Animals 2021, 11(1), 158; https://doi.org/10.3390/ani11010158 - 12 Jan 2021
Cited by 83 | Viewed by 34331
Abstract
A high proportion of dogs and cats are fearful during veterinary visits, which in some cases may escalate into aggression. Here, we discuss factors that contribute to negative emotions in a veterinary setting and how these can be addressed. We briefly summarise the [...] Read more.
A high proportion of dogs and cats are fearful during veterinary visits, which in some cases may escalate into aggression. Here, we discuss factors that contribute to negative emotions in a veterinary setting and how these can be addressed. We briefly summarise the available evidence for the interventions discussed. The set-up of the waiting area (e.g., spatial dividers; elevated places for cat carriers), tailoring the examination and the treatment to the individual, considerate handling (minimal restraint when possible, avoiding leaning over or cornering animals) and offering high-value food or toys throughout the visit can promote security and, ideally, positive associations. Desensitisation and counterconditioning are highly recommended, both to prevent and address existing negative emotions. Short-term pain from injections can be minimised by using tactile and cognitive distractions and topical analgesics, which are also indicated for painful procedures such as ear cleanings. Recommendations for handling fearful animals to minimise aggressive responses are discussed. However, anxiolytics or sedation should be used whenever there is a risk of traumatising an animal or for safety reasons. Stress-reducing measures can decrease fear and stress in patients and consequently their owners, thus strengthening the relationship with the clients as well as increasing the professional satisfaction of veterinary staff. Full article
(This article belongs to the Section Animal Welfare)
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16 pages, 866 KiB  
Article
SARS-CoV-2 in Danish Mink Farms: Course of the Epidemic and a Descriptive Analysis of the Outbreaks in 2020
by Anette Boklund, Anne Sofie Hammer, Michelle Lauge Quaade, Thomas Bruun Rasmussen, Louise Lohse, Bertel Strandbygaard, Charlotte Sværke Jørgensen, Ann Sofie Olesen, Freja Broe Hjerpe, Heidi Huus Petersen, Tim Kåre Jensen, Sten Mortensen, Francisco F. Calvo-Artavia, Stine Kjær Lefèvre, Søren Saxmose Nielsen, Tariq Halasa, Graham J. Belsham and Anette Bøtner
Animals 2021, 11(1), 164; https://doi.org/10.3390/ani11010164 - 12 Jan 2021
Cited by 83 | Viewed by 8966
Abstract
SARS-CoV-2 infection is the cause of COVID-19 in humans. In April 2020, SARS-CoV-2 infection in farmed mink (Neovision vision) occurred in the Netherlands. The first outbreaks in Denmark were detected in June 2020 in three farms. A steep increase in the [...] Read more.
SARS-CoV-2 infection is the cause of COVID-19 in humans. In April 2020, SARS-CoV-2 infection in farmed mink (Neovision vision) occurred in the Netherlands. The first outbreaks in Denmark were detected in June 2020 in three farms. A steep increase in the number of infected farms occurred from September and onwards. Here, we describe prevalence data collected from 215 infected mink farms to characterize spread and impact of disease in infected farms. In one third of the farms, no clinical signs were observed. In farms with clinical signs, decreased feed intake, increased mortality and respiratory symptoms were most frequently observed, during a limited time period (median of 11 days). In 65% and 69% of farms, virus and sero-conversion, respectively, were detected in 100% of sampled animals at the first sampling. SARS-CoV-2 was detected, at low levels, in air samples collected close to the mink, on mink fur, on flies, on the foot of a seagull, and in gutter water, but not in feed. Some dogs and cats from infected farms tested positive for the virus. Chickens, rabbits, and horses sampled on a few farms, and wildlife sampled in the vicinity of the infected farms did not test positive for SARS-CoV-2. Thus, mink are highly susceptible to infection by SARS-CoV-2, but routes of transmission between farms, other than by direct human contact, are unclear. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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17 pages, 1098 KiB  
Review
The Educational Efficacy of Humane Teaching Methods: A Systematic Review of the Evidence
by Miriam A. Zemanova and Andrew Knight
Animals 2021, 11(1), 114; https://doi.org/10.3390/ani11010114 - 7 Jan 2021
Cited by 22 | Viewed by 8078
Abstract
Humane alternatives to harmful educational animal use include ethically-sourced cadavers, models, mannequins, mechanical simulators, videos, computer and virtual reality simulations, and supervised clinical and surgical experiences. In many life and health sciences courses, however, traditional animal use persists, often due to uncertainty about [...] Read more.
Humane alternatives to harmful educational animal use include ethically-sourced cadavers, models, mannequins, mechanical simulators, videos, computer and virtual reality simulations, and supervised clinical and surgical experiences. In many life and health sciences courses, however, traditional animal use persists, often due to uncertainty about the educational efficacy of humane alternatives. The most recent comprehensive reviews assessing learning outcomes of humane teaching methods, in comparison to harmful animal use, were published more than 10 years ago. Therefore, we aimed to collate and analyse the combined evidence from recent and older studies about the efficacy of humane teaching methods. Using specific search terms, we systematically searched the Web of Science, SCOPUS, and EMBASE databases for relevant educational studies. We extracted information on publication years, the country in which the study was conducted, field, humane teaching methods, form of learning outcome assessment, and the learning outcome of the humane teaching methods, in comparison with harmful animal use. We found 50 relevant studies published from 1968–2020, primarily stemming from the USA, UK, and Canada. Humane teaching methods produced learning outcomes superior (30%), equivalent (60%), or inferior (10%) to those produced by traditional harmful animal use. In conclusion, a wide-spread implementation of humane teaching methods would not only preserve learning outcomes, but may in fact be beneficial for animals, students, educators, and institutions. Full article
(This article belongs to the Section Animal Welfare)
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12 pages, 1234 KiB  
Article
Increasing Hepatitis E Virus Seroprevalence in Domestic Pigs and Wild Boar in Bulgaria
by Katerina Takova, Tsvetoslav Koynarski, Ivan Minkov, Zdravka Ivanova, Valentina Toneva and Gergana Zahmanova
Animals 2020, 10(9), 1521; https://doi.org/10.3390/ani10091521 - 28 Aug 2020
Cited by 24 | Viewed by 5269
Abstract
(1) Background: Hepatitis E virus (HEV) is a causative agent of acute viral hepatitis, predominantly transmitted by the fecal–oral route. In developed countries, HEV is considered to be an emerging pathogen since the number of autochthonous cases is rising. Hepatitis E is a [...] Read more.
(1) Background: Hepatitis E virus (HEV) is a causative agent of acute viral hepatitis, predominantly transmitted by the fecal–oral route. In developed countries, HEV is considered to be an emerging pathogen since the number of autochthonous cases is rising. Hepatitis E is a viral disease with a proven zoonotic potential for some of its genotypes. The main viral reservoirs are domestic pigs and wild boar. Consumption of undercooked meat, as well as occupational exposure, are key factors for the spread of HEV. In order to evaluate the risks of future viral evolution, a detailed examination of the ecology and distribution of the virus is needed. The aim of the present study is to investigate the prevalence of anti-HEV IgG Ab in domestic pigs and wild boar in Bulgaria; (2) Methods: In this study, during the period of three years between 2017 and 2019, 433 serum samples from 19 different pig farms and 1 slaughterhouse were collected and analyzed. In addition, 32 samples from wild boar were also collected and analyzed during the 2018–2019 hunting season. All samples were analyzed by commercial indirect ELISA; (3) Results: Overall, HEV seroprevalence was 60% (95% CI 42.7–77.1) in domestic pigs and 12.5% (4/32) in wild boar. The observed seroprevalence of the slaughter-aged pigs was 73.65% (95% Cl 58.7–87.3). Prevalence in domestic pigs was significantly higher in the samples collected during 2019 (98% (95% Cl 96.1–99.9)) compared to those collected during 2017 (45.33% (95% CI 2.7–87.3)) and 2018 (38.46% (95% CI 29.1–49.7.); (4) Conclusions: Our findings suggest that domesticated pigs and wild boar might be the reason for the increased HEV transmission across Bulgaria. The genotypic characterization of HEV found in pigs, wild boar and humans will give a more accurate view of the zoonotic transmission of this virus. Full article
(This article belongs to the Special Issue Zoonoses: Wild and Domestic Animal Interaction)
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10 pages, 1266 KiB  
Article
Behavioural Evaluation of a Leash Tension Meter Which Measures Pull Direction and Force during Human–Dog On-Leash Walks
by Hao-Yu Shih, Fillipe Georgiou, Robert A. Curtis, Mandy B. A. Paterson and Clive J. C. Phillips
Animals 2020, 10(8), 1382; https://doi.org/10.3390/ani10081382 - 10 Aug 2020
Cited by 13 | Viewed by 10671
Abstract
Leash tension forces exerted by dog and handler during walks affect their welfare. We developed a novel ambulatory measurement device using a load cell and a tri-axial accelerometer to record both the tension and direction of forces exerted on the leashes. Data were [...] Read more.
Leash tension forces exerted by dog and handler during walks affect their welfare. We developed a novel ambulatory measurement device using a load cell and a tri-axial accelerometer to record both the tension and direction of forces exerted on the leashes. Data were relayed telemetrically to a laptop for real time viewing and recording. Larger and heavier dogs exerted higher leash tension but had a lower pulling frequency than their smaller and lighter conspecifics. This pattern was observed in the reactional forces of handlers. Young dogs pulled more frequently during walks, which was also mirrored in handlers’ pulling. Well-behaved dogs created lower leash tension, but handlers did not respond with lower forces. This novel method of recording leash tension will facilitate real-time monitoring of the behaviour of dogs and their handlers during walks. Full article
(This article belongs to the Special Issue Companion Animal Cognition, Communication, and Behavior)
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21 pages, 1037 KiB  
Article
Dogs Don’t Die Just in Hot Cars—Exertional Heat-Related Illness (Heatstroke) Is a Greater Threat to UK Dogs
by Emily J. Hall, Anne J. Carter and Dan G. O’Neill
Animals 2020, 10(8), 1324; https://doi.org/10.3390/ani10081324 - 31 Jul 2020
Cited by 16 | Viewed by 21420
Abstract
Heat-related illness will affect increasing numbers of dogs as global temperatures rise unless effective mitigation strategies are implemented. This study aimed to identify the key triggers of heat-related illness in dogs and investigate canine risk factors for the most common triggers in UK [...] Read more.
Heat-related illness will affect increasing numbers of dogs as global temperatures rise unless effective mitigation strategies are implemented. This study aimed to identify the key triggers of heat-related illness in dogs and investigate canine risk factors for the most common triggers in UK dogs. Using the VetCompassTM programme, de-identified electronic patient records of 905,543 dogs under primary veterinary care in 2016 were reviewed to identify 1259 heat-related illness events from 1222 dogs. Exertional heat-related illness was the predominant trigger (74.2% of events), followed by environmental (12.9%) and vehicular confinement (5.2%). Canine and human risk factors appear similar; young male dogs had greater odds of exertional heat-related illness, older dogs and dogs with respiratory compromise had the greatest odds of environmental heat-related illness. Brachycephalic dogs had greater odds of all three types of heat-related illness compared with mesocephalic dogs. The odds of death following vehicular heat-related illness (OR 1.47, p = 0.492) was similar to that of exertional heat-related illness. In the UK, exertional heat-related illness affects more dogs, and kills more dogs, than confinement in a hot vehicle. Campaigns to raise public awareness about heat-related illness in dogs need to highlight that dogs don’t die just in hot cars. Full article
(This article belongs to the Section Companion Animals)
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16 pages, 307 KiB  
Article
Potential of Recycling Cauliflower and Romanesco Wastes in Ruminant Feeding: In Vitro Studies
by Trinidad de Evan, Andrea Vintimilla, Eduarda Molina-Alcaide, María José Ranilla and María Dolores Carro
Animals 2020, 10(8), 1247; https://doi.org/10.3390/ani10081247 - 22 Jul 2020
Cited by 11 | Viewed by 4006
Abstract
The nutritive values for ruminants of cauliflower (CAU) and Romanesco (ROM) wastes (leaves, stems and sprouts) were assessed by analyzing their chemical composition, in vitro ruminal fermentation, and in vitro intestinal digestibility. In addition, the in vitro ruminal fermentation of diets containing increasing [...] Read more.
The nutritive values for ruminants of cauliflower (CAU) and Romanesco (ROM) wastes (leaves, stems and sprouts) were assessed by analyzing their chemical composition, in vitro ruminal fermentation, and in vitro intestinal digestibility. In addition, the in vitro ruminal fermentation of diets containing increasing amounts of CAU was studied. The dry matter (DM) content of leaves, stems and sprouts of both vegetables was lower than 10%, but they contained high crude protein (CP; 19.9 to 33.0%) and sugar (16.3 to 28.7%) levels, and low neutral detergent fiber (21.6 to 32.3%). Stems and sprouts were more rapidly and extensively fermented in the rumen than leaves, but there were only minor differences the fermentation profiles of both vegetables. The estimated metabolizable energy content ranged from 9.3 (leaves) to 10.8 (sprouts) MJ/kg DM. The CP rumen degradability (12-h in situ incubations) was greater than 80.0% for all fractions, and the in vitro intestinal digestibility of CP ranged from 85.7 to 93.2%. The inclusion of up to 24% of dried CAU in the concentrate of a mixed diet (40:60 alfalfa hay:concentrate) increased the in vitro rumen fermentation of the CAU diet, but did not affect methane (CH4) production, indicating the lack of antimethanogenic compounds in CAU. Full article
(This article belongs to the Section Animal Nutrition)
10 pages, 415 KiB  
Article
Effects of Dietary β-Mannanase Supplementation on Growth Performance, Apparent Total Tract Digestibility, Intestinal Integrity, and Immune Responses in Weaning Pigs
by Jae-Cheol Jang, Kwang Ho Kim, Young Dal Jang and Yoo Yong Kim
Animals 2020, 10(4), 703; https://doi.org/10.3390/ani10040703 - 17 Apr 2020
Cited by 10 | Viewed by 3876
Abstract
The experiment aimed to investigate the effects of dietary β-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of nutrients, intestinal integrity, and the immunological and oxidative stress parameters in weaning pigs. A total of 64 newly weaning pigs (initial body weight: [...] Read more.
The experiment aimed to investigate the effects of dietary β-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of nutrients, intestinal integrity, and the immunological and oxidative stress parameters in weaning pigs. A total of 64 newly weaning pigs (initial body weight: 6.96 ± 0.70 kg) were allotted to two dietary treatments in eight replicates per treatment with four pigs per pen based on body weight and sex. Dietary treatments were 1.) CON (control: corn-soybean meal based basal diet) and 2.) β-mannanase (basal diet +0.06% β-mannanase). The β-mannanase supplementation did not affect growth performance, concentrations of acute phase protein, superoxide dismutase and glutathione peroxidase. However, the pigs fed the β-mannanase-supplemented diet had greater ATTD of ether extract, jejunum villus height, and villus height-to-crypt depth ratio, and lower crypt depth compared with those fed the CON diet (p < 0.05). The pigs fed the β-mannanase-supplemented diet tended to have the lower count of E. coli in cecum than those fed the CON diet (p = 0.08). In conclusion, dietary β-mannanase supplementation did not affect growth performance, immune response and oxidative stress of weaning pigs, whereas it increased fat digestibility and had positive effects on intestinal integrity and cecum microflora by reducing the count of E.coli. Full article
(This article belongs to the Section Animal Nutrition)
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12 pages, 1075 KiB  
Article
Picture Perfect Pups: How Do Attributes of Photographs of Dogs in Online Rescue Profiles Affect Adoption Speed?
by Mizuho Nakamura, Navneet Dhand, Bethany J. Wilson, Melissa J. Starling and Paul D. McGreevy
Animals 2020, 10(1), 152; https://doi.org/10.3390/ani10010152 - 16 Jan 2020
Cited by 9 | Viewed by 8352
Abstract
To increase the public’s awareness of and exposure to animals needing homes, PetRescue, Australia’s largest online directory of animals in need of adoption, lists all currently available animals from rescue and welfare shelters nationwide. The current study examined the photographs in the PetRescue [...] Read more.
To increase the public’s awareness of and exposure to animals needing homes, PetRescue, Australia’s largest online directory of animals in need of adoption, lists all currently available animals from rescue and welfare shelters nationwide. The current study examined the photographs in the PetRescue online profiles of the three most common breeds within these data, namely, Staffordshire bull terriers (n = 3988), Labrador retrievers (n = 2246), and Jack Russell terriers (n = 2088), to identify the inferred preferences of potential adopters. By investigating the attributes of these photographs, we were able to identify visual risk factors associated with protracted lengths of stay (LOS). The longest stays were associated with dogs with erect ears and those photographed in a natural environment, i.e., 18.32 days and 19.57 days, respectively. Dogs photographed in a kennel and with mouths closed had the shortest LOS, i.e., 11.54 d and 14.44 d, respectively. Heightened awareness of the roles of photographic attributes in generating interest among potential adopters may increase the speed of adoption by guiding the creation of online profiles and selection of photos to optimise the promotion of dogs at risk of long stays. Full article
(This article belongs to the Section Companion Animals)
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