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Plant Tissue Culture IV
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Plant Tissue Culture IV

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: closed (20 June 2024) | Viewed by 7198

Special Issue Editor

Dr. Iyyakkannu Sivanesan

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Guest Editor
Department of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea
Interests: plant tissue culture; secondary metabolites; hydroponics; environmental protection
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant tissue culture generally refers to the in vitro culture of explants on artificial media under aseptic conditions. In vitro culture procedures include a selection of explants, disinfection, media preparation, maintenance of culture conditions, callus formation, organogenesis, and hardening. It is a vital method for studying plants' morphogenesis, cell signaling, physiology, and molecular biology. This technique has various applications, such as producing artificial seeds, hybrids, disease- and stress-resistant plants, genetic resource conservation, secondary metabolites, and genetically uniform plants. This Special Issue aims to cover research on clonal propagation, cryopreservation, embryogenesis, organogenesis, somaclonal variation, temporary immersion systems, transgenics, protoplast culture, hairy root culture, and secondary metabolites production.

Dr. Iyyakkannu Sivanesan
Guest Editor

Manuscript Submission Information

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Keywords

  • secondary metabolites
  • cell suspension culture
  • genetic transformation
  • hairy root culture
  • germplasm conservation
  • micropropagation
  • somatic embryogenesis
  • mutation
  • protoplast culture
  • bioreactor
  • sterilization methods
  • light quality

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Published Papers (6 papers)

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Research

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15 pages, 1733 KiB  
Article
A First Approach for the In Vitro Cultivation, Storage, and DNA Barcoding of the Endangered Endemic Species Euonymus koopmannii
by Balnur Kali, Sara Bekkuzhina, Dilnur Tussipkan and Shuga Manabayeva
Plants 2024, 13(16), 2174; https://doi.org/10.3390/plants13162174 - 6 Aug 2024
Viewed by 507
Abstract
Euonymus koopmannii is a rare and protected species in Kazakhstan, valued for its ecological role in soil stabilization and its ornamental properties. This study presents the first use of micropropagation and phylogenetic analysis for the endemic plant E. koopmannii. Seedlings of E. koopmannii [...] Read more.
Euonymus koopmannii is a rare and protected species in Kazakhstan, valued for its ecological role in soil stabilization and its ornamental properties. This study presents the first use of micropropagation and phylogenetic analysis for the endemic plant E. koopmannii. Seedlings of E. koopmannii proved to be more effective than internodes as primary explants for plant micropropagation of in vitro culture, with a multiplication coefficient of 28.5 from seedlings and 6.1 from internodes. On MSR I medium supplemented with 0.5 mg/L IBA and 0.05 mg/L IAA, a higher success rate of 67% was achieved for root formation of test tube-grown E. koopmannii plants. Using mannitol as an osmotic agent at a concentration of 8 mg/L prolonged the storage time of E. koopmannii under slow growth conditions when compared to CCC and abscisic acid. Phylogenetic relationships and species identification were analyzed using four DNA-barcoding markers, comparing E. koopmannii with species from NCBI. All candidate barcoding markers showed sufficient levels of interspecific genetic variation among Euonymus species. In addition, ITS region and rbcL gene sequences effectively distinguished E. koopmannii from other species. These results provide fundamental information that will be valuable for future biotechnological and molecular studies. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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10 pages, 1043 KiB  
Article
Effect of Light Quality on the seed Germination and Development of Coffee Seedlings (Coffea arabica)
by Ana Julia Cardoso-Magaña, Mayra Itzcalotzin Montero-Cortés, Joaquin Alejandro Qui-Zapata, Arturo Moises Chávez-Rodríguez, Norma Alejandra Mancilla-Margalli, Julio Cesar López-Velázquez, Vania Sbeyde Farias-Cervantes, Isaac Andrade-González, Diana Maria Rivera-Rodríguez and Fabiola Bejarano-Rebolledo
Plants 2024, 13(13), 1772; https://doi.org/10.3390/plants13131772 - 27 Jun 2024
Viewed by 1218
Abstract
Coffee (Coffea arabica) cultivation is vital to the global economic, social and cultural life of farmers. However, senescent and disease-susceptible plantations affect coffee productivity. Therefore, it is crucial to improve biotechnological strategies such as micropropagation to increase the number of plants [...] Read more.
Coffee (Coffea arabica) cultivation is vital to the global economic, social and cultural life of farmers. However, senescent and disease-susceptible plantations affect coffee productivity. Therefore, it is crucial to improve biotechnological strategies such as micropropagation to increase the number of plants for replanting. In this study, the dark condition (T1) and different light qualities (T0-white light 400–700 nm; T2-red light 660 nm and T3-blue light 460 nm) were evaluated to optimize the in vitro propagation of 4 and 9 month-old coffee seeds. The results showed that red light had the highest percentage, an outstanding germination rate index, which may suggest that in the case of coffee seeds could be involved phytochromes that promote germination in a red light quality. In summary, the ideal conditions for in vitro micropropagation of coffee are under white and red light condition. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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7 pages, 1222 KiB  
Communication
Micropropagation and Shoot Tip Cryopreservation of ‘Sunny Gold’ Freesia
by Jinjoo Bae, Jae-Young Song, Young-Yi Lee, Ye-ji Lee, Youn Jung Choi, Oh-Keun Kwon, Sung-Hee Nam, Ho-sun Lee, Seok Cheol Kim and Ji-Won Han
Plants 2024, 13(12), 1655; https://doi.org/10.3390/plants13121655 - 14 Jun 2024
Viewed by 738
Abstract
Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for ‘Sunny Gold’ Freesia, starting from effective in vitro initiation and proliferation using various plant [...] Read more.
Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for ‘Sunny Gold’ Freesia, starting from effective in vitro initiation and proliferation using various plant growth regulator combinations. We also assess the impact of subculture on regrowth rates after cryopreservation. The shoot tips were successfully initiated in vitro after sterilization. The shoots were multiplied an average of three times in media containing N6-benzyladenine and kinetin. The regrowth rates of non-cryopreserved shoot tips excised from different subculture cycles did not differ significantly, with rates of 44% observed for plants from more than five subcultures and 47% for those from three subcultures. However, only the shoot tips excised from cultures subjected to three subculture cycles were able to recover after cryopreservation, with a regrowth rate of 31%. Our findings lay the groundwork for the development of an efficient cryopreservation protocol for freesias in the future. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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13 pages, 2081 KiB  
Article
Meta-Topolin as an Effective Benzyladenine Derivative to Improve the Multiplication Rate and Quality of In Vitro Axillary Shoots of Húsvéti Rozmaring Apple Scion
by Neama Abdalla and Judit Dobránszki
Plants 2024, 13(11), 1568; https://doi.org/10.3390/plants13111568 - 6 Jun 2024
Cited by 1 | Viewed by 760
Abstract
In vitro mass propagation of apple plants plays an important role in the rapid multiplication of genetically uniform, disease-free scions and rootstocks with desired traits. Successful micropropagation of apple using axillary shoot cultures is influenced by several factors, the most critical of which [...] Read more.
In vitro mass propagation of apple plants plays an important role in the rapid multiplication of genetically uniform, disease-free scions and rootstocks with desired traits. Successful micropropagation of apple using axillary shoot cultures is influenced by several factors, the most critical of which is the cytokinin included in the culture medium. The impact of medium composition from single added cytokinins on shoot proliferation of apple scion Húsvéti rozmaring cultured on agar-agar gelled Murashige and Skoog medium fortified with indole butyric acid and gibberellic acid was investigated. The optimum concentration for efficient shoot multiplication differs according to the type of cytokinin. The highest significant multiplication rate (5.40 shoots/explant) was achieved using 2.0 μM thidiazuron while the longest shoots (1.80 cm) were observed on the medium containing benzyladenine at a concentration of 2.0 μM. However, application of either thidiazuron or benzyladenine as cytokinin source in the medium resulted in shoots of low quality, such as stunted and thickened shoots with small leaves. In the case of benzyladenine riboside, the 8 μM concentration was the most effective in increasing the multiplication rate (4.76 shoots/explant) but caused thickened stem development with tiny leaves. In the present study, meta-topolin was shown to be the most effective cytokinin that could be applied to induce sufficient multiplication (3.28 shoots/explant) and high-quality shoots along with shoot lengths of 1.46 cm when it was applied at concentrations of 4 μM. However, kinetin was the least active cytokinin; it practically did not induce the development of new shoots. The superior cytokinin for in vitro axillary shoot development of apple scion Húsvéti rozmaring with high-quality shoots was the meta-topolin, but it may be different depending on the variety/genotype under study. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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13 pages, 1395 KiB  
Article
Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.
by Yaowapha Jirakiattikul, Srisopa Ruangnoo, Kanokwan Sangmukdee, Kornkanok Chamchusri and Panumart Rithichai
Plants 2024, 13(11), 1450; https://doi.org/10.3390/plants13111450 - 23 May 2024
Viewed by 673
Abstract
Plumbago indica L. contains a valuable bioactive compound called plumbagin. Elicited regenerated shoots grown in vitro could be another source of high-yielding plumbagin. The purpose of this investigation was to examine the effects of elicitor type and concentration, as well as elicitation period, [...] Read more.
Plumbago indica L. contains a valuable bioactive compound called plumbagin. Elicited regenerated shoots grown in vitro could be another source of high-yielding plumbagin. The purpose of this investigation was to examine the effects of elicitor type and concentration, as well as elicitation period, on plumbagin content in in vitro-regenerated shoots of P. indica. Nodal explants were cultured on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine (BA) in combination with 0–150 mg/L yeast extract or 50–150 µM salicylic acid for four weeks. Plumbagin levels of 3.88 ± 0.38% and 3.81 ± 0.37% w/w g dry extract were achieved from the 50 and 100 mg/L yeast extract-elicited shoots, which were higher than the value obtained for the control. However, the addition of salicylic acid did not increase the plumbagin content. In the elicitation period experiment, nodal explants were cultured on MS medium supplemented with 1 mg/L BA and 50 mg/L yeast extract for durations of three, four and five weeks. The 4-week yeast extract-elicited shoot had a maximum plumbagin content of 3.22 ± 0.12% w/w g dry extract, greater than that of the control. In summary, the plumbagin content of the in vitro P. indica shoots was enhanced by 4-week elicitation using 50 mg/L yeast extract. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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Review

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19 pages, 1408 KiB  
Review
A Comprehensive Review Uncovering the Challenges and Advancements in the In Vitro Propagation of Eucalyptus Plantations
by Vikas Sharma, Ankita, Arun Karnwal, Shivika Sharma, Barkha Kamal, Vikash S. Jadon, Sanjay Gupta and Iyyakkannu Sivanasen
Plants 2023, 12(17), 3018; https://doi.org/10.3390/plants12173018 - 22 Aug 2023
Cited by 3 | Viewed by 2072
Abstract
The genus Eucalyptus is a globally captivated source of hardwood and is well known for its medicinal uses. The hybrid and wild species of Eucalyptus are widely used as exotic plantations due to their renowned potential of adapting to various systems and sites, [...] Read more.
The genus Eucalyptus is a globally captivated source of hardwood and is well known for its medicinal uses. The hybrid and wild species of Eucalyptus are widely used as exotic plantations due to their renowned potential of adapting to various systems and sites, and rapid large-scale propagation of genetically similar plantlets, which further leads to the extensive propagation of this species. Tissue culture plays a crucial role in the preservation, propagation, and genetic improvement of Eucalyptus species. Despite unquestionable progression in biotechnological and tissue culture approaches, the productivity of plantations is still limited, often due to the low efficiency of clonal propagation from cuttings. The obtained F1 hybrids yield high biomass and high-quality low-cost raw material for large-scale production; however, the development of hybrid, clonal multiplication, proliferation, and post-developmental studies are still major concerns. This riveting review describes the problems concerning the in vitro and clonal propagation of Eucalyptus plantation and recent advances in biotechnological and tissue culture practices for massive and rapid micropropagation of Eucalyptus, and it highlights the Eucalyptus germplasm preservation techniques. Full article
(This article belongs to the Special Issue Plant Tissue Culture IV)
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