Silver oxide (Ag
2O) particles are wonderful candidates due to their unique properties, and their use in a wide range of research, industrial and biomedical applications is rapidly increasing. This makes it fundamental to develop simple, environmentally friendly methods with possible scaling. Herein, sodium borohydride and
Datura innoxia leaf extract were applied as chemical and biological stabilizing and reducing agents to develop Ag
2O particles. The primary aim was to evaluate the anticancer and antiviral activity of Ag
2O particles prepared via two methods. XRD, UV-visible and SEM analyses were used to examine the crystallite structure, optical properties and morphology, respectively. The resulting green-synthesized Ag
2O particles exhibited small size, spherically agglomerated shape, and high anticancer and antiviral activities compared to chemically synthesized Ag
2O particles. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium-bromide) assay of green-synthesized Ag
2O particles showed high anticancer activity against MCF-7 cells with IC
50 = 17.908 µg/mL compared to chemically synthesized Ag
2O particles with IC
50 = 23.856 µg/mL. The antiviral activity of green-synthesized Ag
2O particles and chemically synthesized Ag
2O particles was also evaluated by a plaque-forming assay, and green-synthesized Ag
2O particles showed higher antiviral ability with IC
50 = 0.618 µg/mL as compared to chemically synthesized Ag
2O particles with IC
50 = 6.129 µg/mL. We propose the use of green-synthesized Ag
2O particles in cancer treatment and drug delivery.
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