Search Results (2,148)

This article presents a novel microstrip patch sensor featuring four rectangular rings represented by single complementary split-ring resonance (SC-SRR) to calculate the complex permittivity of saline solutions within the range of 0 ppt to 100 ppt. This sensor operates via the turbulence technique, utilizing its resonant properties as indicators to find the parameters of the liquid under test (LUT), which arise due to the variations in the salt concentration altering the complex permittivity. This alteration influences the resonant frequency (f_r), reflection coefficient (S₁₁), and quality factor (Q). The sensor was designed by using a high-frequency structure simulator (HFSS) and by using an FR-4 substrate and a Teflon box with a height of 1.4 mm and 13.7 mm, respectively. The values of S₁₁ at resonance frequency were −34.48 dB, and 2.1328 GHz, respectively. A computer numerical control (CNC) machine was used to fabricate the sensor and Teflon box, and the Teflon box was situated above the four rings to create a strong interaction between the induced electric field and the LUT, thereby achieving high sensitivity in a non-contacting and non-destructive manner. The measurement and simulation results were consistent and aligned with those of Klien and Meissner (in comparison to the theoretical values derived from the single and double Debye models). We derived numerical equations for the conductivity (S/m), dielectric constant permittivity, and concentrations (ppt) using curve fitting origin software, and the results are in good agreement. Due to its performance, we expect that the proposed sensor could be used in agricultural applications to identify freshwater and in medical applications to detect the concentration of salt in saliva or blood and to identify diseases, in addition to many other applications involving mixed liquids. Full article

Effective wastewater management is a critical environmental challenge, particularly in industrial regions like Faisalabad, where untreated textile effluents contribute to severe water pollution. This study evaluates the potential of phytoremediation using floating aquatic plants—Eichhornia crassipes (water hyacinth), Pistia stratiotes (water lettuce), and Lemna minor (common duckweed)—for the treatment of industrial textile wastewater. A controlled laboratory-scale experiment was conducted to assess pollutant removal efficiency over a 10-day retention period. The initial effluent concentrations of key parameters were measured before treatment to establish baseline conditions. The results demonstrated that Eichhornia crassipes exhibited the highest removal efficiency, achieving reductions of 36.12% (TDS), 36.14% (EC), 36.30% (salinity), 6.12% (pH), 34.30% (total hardness), and 44.52% (chloride). Furthermore, Pistia stratiotes and Lemna minor were particularly effective in removing nitrate (99.76%), ammonium (52.11%), and sodium adsorption ratio (46.29%), indicating species-specific phytoremediation potential. These findings highlight the viability of a low-cost, eco-friendly, and sustainable nature-based solution for wastewater treatment in industrial clusters, offering a practical alternative to conventional treatment technologies. Full article

The blood–brain barrier (BBB) is key to the regular functioning of the central nervous system. The dysfunction of the BBB has been described in various neurological disorders, including schizophrenia. Schizophrenia (SCZ) is a chronic psychiatric disorder described by hallucinations, delusions, and negative symptoms. The Olanzapine (OLZ) drug is an electroactive species, and its levels can be monitored using electrochemical sensors. The detection of OLZ was demonstrated previously by using electrochemical sensors, and this technique can be used to monitor the levels of OLZ in real time. The challenge is to identify the permeability of OLZ through the BBB, so a replica model was designed with the BBB based on a Transwell membrane seeded with endothelial cells. A microfabricated electrode consisting of a 3 mm Au disk was modified with platinum black; this enables higher selectivity of electrochemical signals from OLZ. The dose–response of OLZ was characterized in phosphate buffer saline solution (10 mM, pH 7.4) by adding 20–200 nM (in steps 20) of OLZ stock solution. The observed chronoamperometric electrochemical signals showed an increasing current at 0.45 V vs. Ag/AgCl with an increasing OLZ concentration. The controls for the experiments were performed in phosphate-buffered saline solution (10 mM, pH 7.4). The detection limit was calculated as 9.96 ± 7.35 × 10⁻⁶ nM from the calibration curve. The membrane permeability of the OLZ drug tested with five SCZ patients was monitored by studying the TEER measurements and permeability rate constant data. Full article

After weaning, piglets no longer consume breast milk, and their immune system is not yet fully developed. At this time, if weaned piglets are infected with E. coli, their subsequent growth will be seriously affected. In the present study, 48 healthy 28-day-old weaned piglets (6.65 ± 1.19 kg, Duroc × Landrace × Large White) were randomly divided into an LPS group and control group. Piglets in the LPS group were intraperitoneally injected with an LPS solution (LPS was dissolved in sterile saline to form a solution of 100 μg/mL and injected at a dose of 1 mL per kilogram of body weight) for 13 consecutive days. Piglets in the control group were injected with the same volume of sterile saline. On days 1, 5, 9, and 13 of the experiment, six piglets from each group were randomly selected for dissection, the blood and heart samples were collected, and then cardiac function-related indicators were detected. A portion of the heart tissue was fixed in 4% paraformaldehyde and further used to make paraffin sections; then, hematoxylin–eosin (H&E) staining was performed. Masson staining was used to detect the changes in collagen fibers in the hearts. The other parts of the heart tissues were frozen in liquid nitrogen and stored in a refrigerator at −80 °C for the detection of tissue antioxidant indices. The mRNA expression levels of the toll-like receptor 4 (TLR4) signaling pathway, transforming growth factor-β (TGF-β) signaling pathway, and inflammatory cytokines in heart tissues were detected by real-time PCR. The results showed that catalase (CAT) and superoxide dismutase (SOD) contents in the heart tissue homogenates increased significantly on days 1 and 5 in LPS-induced piglets (p < 0.01, p < 0.05), while total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) contents decreased significantly on day 5 (p < 0.05). On day 5, the contents of serum cardiac function indicators lactate dehydrogenase (LDH), creatine kinase isoenzymes (CK-MB), and cardiac troponin I (cTn-I) were significantly increased in LPS-induced piglets (p < 0.01). On the 1st and 5th days, the heart tissue showed obvious pathological damage, which was manifested as the disordered arrangement of myocardial fibers, depression of myocardial cells, infiltration of inflammatory factors, congestion of capillaries, and significant increase in cardiac collagen fibers. On the 1st day, the mRNA expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) were significantly increased in LPS-induced piglets with heart injury (p < 0.01). On the 5th day, the mRNA expression levels of the TLR4 signaling pathway [TLR4, myeloid differentiation primary response gene 88 (MyD88), nuclear factor kappa-B (NF-κB)], TNF-α, and interleukin 10 (IL-10) were also significantly increased in LPS-induced piglets with heart injury (p < 0.01, p < 0.05). The mRNA expression levels of the TGF-β signaling pathway (TGF-β, Smad2, and Smad4) in cardiac fibrosis-related genes were significantly increased on days 5 and 9 (p < 0.01, p < 0.05). The mRNA expression levels of Smad3 and Smad7 in cardiac fibrosis-related genes were also significantly increased on day 9 (p < 0.01). These results indicate that oxidative stress occurs in the heart tissue of LPS-induced piglets on the 1st and 5th days, leading to cardiac tissue damage. However, on the 9th and 13th days, the degree of heart damage in the piglets was less than that on the 1st and 5th days, which may be due to the tolerance of piglets’ tissues and organs because of multiple same-dose LPS stimulations. Full article

This study investigated the effect of total substitution of NaCl with KCl and MgCl₂ on the physicochemical, microbiological, and textural characteristics of pickled apples during 35 days of fermentation. The results showed that the pH for all brine-pickled apples decreased significantly (p < 0.05) during the fermentation process. The highest quantity (1077.59 ± 17.56 mg lactic acid/100 g product) of lactic acid was detected on the 28th day for the samples fermented with NaCl. The concentration of metallic ions (Na⁺, K⁺, Mg²⁺) in the brine-pickled apple samples showed a peak on the 14th day of fermentation, followed by a decrease on the 21st day. The antioxidant activity for all types of saline solutions increased as fermentation progressed. The total LAB count increased rapidly until the seventh day for all the samples. At the end of the storage period, a decrease in LAB count was observed for all tested samples. The obtained results revealed that replacing NaCl with KCl or MgCl₂ led to small changes in the characteristics of the pickled apples, and is thus a promising option for dietary sodium reduction. Full article

Vacuum membrane distillation (VMD) is a promising process for water desalination. However, it suffers some obstacles, such as fouling and wetting, due to the inadequate hydrophobicity of the membrane and high vacuum pressure on the permeate side. Therefore, improving surface hydrophobicity and roughness is important. In this study, the effect of 1H,1H,2H,2H-Perfluorodecyltriethoxysilane (PFTES) on the morphology and performance of CNM/PAC/PVDF membranes at various concentrations was investigated for the first time. Membrane characteristics such as FTIR, XRD, FE-SEM, EDX, contact angle, and hydrophobicity before and after modification were analyzed and tested using VMD for water desalination. The results showed that the membrane coated with 1 wt.% PFTES had a higher permeate flux and lower rejection than the membranes coated with the 2 wt.% PFTES. The 2 wt.% PFTES enhanced the contact angle to 117° and increased the salt rejection above 99.9%, with the permeate flux set to 23.2 L/m²·h and at a 35 g/L NaCl feed solution, 65 °C feed temperature, a 0.6 L/min feed flow rate, and 21 kPa (abs) vacuum pressure. This means that 2 wt.% PFTES-coated PVDF membranes exhibited slightly lower permeate flux with higher hydrophobicity, salt rejection, and stability over long-term operation. These outstanding results indicate the potential of the novel CNM/PAC/PVDF/PFTES membranes for saline water desalination. Moreover, this study presents useful guidance for the enhancement of membrane structures and physical properties in the field of saline water desalination using porous CNM/PAC/PVDF/PFTES membranes. Full article

The widespread use of gadolinium (Gd) in medical and industrial applications, especially as a contrast agent in magnetic resonance imaging (MRI), has led to its increasing presence in surface waters, disrupting natural geochemical cycles and posing risks to aquatic ecosystems. Addressing this challenge, recent studies have explored the potential of magnetic materials, such as spinel ferrite nanoparticles, in the removal of Gd from contaminated water sources. The present study specifically focused on the use of MnFe₂O₄ nanoparticles to remove Gd from contaminated solutions, employing response surface methodology (RSM) to optimize sorption conditions. Key variables evaluated included salinity (0–30 g/L), initial Gd concentration (1–5 μmol/L), and sorbent dose (20–180 mg/L), at a fixed pH of 6. The results revealed that salinity had a minimal impact on Gd sorption, likely due to the high sorbent mass used. Optimal conditions were identified as a sorbent dose of 165 mg/L, an initial Gd concentration of 1.3 μmol/L, and a salinity level of 13.4 g/L, at pH 6. The process was efficient and rapid, achieving over 90% Gd removal within 1 h in both freshwater and saline conditions, and over 75% removal in mineral water within 3 h. The high efficiency and celerity of this method suggest that MnFe₂O₄ nanoparticles are a promising solution for treating Gd-contaminated hospital effluents. Future research should focus on validating these results in real-world effluent matrices and addressing the environmental and economic aspects of large-scale implementation, thereby contributing to sustainable water remediation strategies. Full article

Uniaxial compression tests were conducted on coal samples subjected to different dry–wet cycling treatments to investigate the damage and degradation mechanisms of coal samples under the dry–wet cyclic action of acidic, high-salinity solutions. The damage process of the coal samples was monitored in situ using acoustic emission (AE). The degradation evolution of the mechanical parameters and macroscopic failure modes with the number of cycles was analyzed. Based on the AE ringing parameters, the RA-AF distribution and the AE fractal dimension’s variation characteristics were studied. Additionally, scanning electron microscopy (SEM) was used to observe the microstructure of the coal samples. The results showed that with the increase in the number of dry–wet cycles, both the peak strength and elastic modulus of the coal samples exhibited varying degrees of degradation, and the failure mode gradually shifted from tensile failure to shear failure. AE ringing counts decreased progressively, while the proportion of shear cracks based on the RA-AF classification increased. At the same time, the mean AE fractal dimension of the coal samples increased, and the fractal dimension decreased with an increase in AE ringing counts. The sharp drop in fractal dimensions could serve as an early warning signal for a major failure in the coal samples. Furthermore, under the influence of dry–wet cycling in acidic, high-salinity solutions, defects such as pores and cracks in the microstructure of the coal samples became more pronounced, and the degradation effect continuously intensified. Full article

The ability to substitute peat use in horticulture with potentially more sustainable alternatives hinges on the local availability of suitable biomass resources and whether these resources can be easily processed to achieve similar agronomic effectiveness to peat. This review estimates potential biomass availability in Ireland by reviewing production statistics and industry reports and identifying current uses and hypothetical processed biomass quantities. Annual estimates of the major biomass resources available in Ireland are 488,935 m³ of woody residues (mainly Sitka spruce pine) and 789,926 m³ of arable straws (from oats, wheat, barley, oil seed rape). The potential major processing pathways for the available biomass are mechanical (extruded, thinscrew, hammer milled, disc refined), carbonization (pyrolysis and hydrothermal carbonization) and composting. This review of the literature indicates that the major challenges to pyrolyzed alternatives in growth media include high alkalinity, high salinity and low water holding capacity. When biomass is processed into fibers, it requires additional processing to address nutrient immobilization (nitrogen and calcium) and the presence of phytotoxic compounds. We discuss possible solutions to these challenges in terms of agronomic management (altering fertigation, irrigation rates etc.), biomass conversion process optimization (changing conditions of processes and applying additives) and novel growth media formulations with various material inputs that complement each other. We conclude that while national alternative biomass resources are available in sufficient volumes to potentially meet growing media requirements, significant further research and demonstration are required to convert these materials to growth media acceptable to both commercial and retail sectors. Research needs to focus on transforming these materials into growth media, and how they will impact agronomic management of crops. Furthermore to this, the optimization of biomass conversion processes and novel formulations incorporating multiple types of biomass need to be the focus as we transition from peat products in professional horticulture. Full article

The rehabilitation of post-mining sites is crucial due to the severe environmental impacts of mining, including land degradation, heavy metal pollution, and loss of biodiversity. Effective reclamation strategies are essential to reverse these impacts and enable sustainable land use. This study presents the possibility of the rehabilitation of a post-mining area in Velenje, Slovenia, using artificial soils made from combustion by-products amended with lignite and organic compost, and explores the potential of lignite mine water for irrigation. This approach introduces an innovative solution that differs from the traditional methods of rehabilitating degraded areas. Physicochemical and phytotoxicity tests were conducted to determine the quality of the soil substitutes. The analysis revealed that the pH, salinity, and chemical composition of soils positively impacted Sinapis alba growth as a test plant, with the most promising compositions containing 20–30% of lignite by weight as a replacement for organic compost. Irrigation water quality parameters, such as electrical conductivity (0.87 dS/m), the sodium absorption ratio (2.09 meq/L), and boron content (0.05 mg/L), indicated a low soil dispersion risk, while the residual sodium carbonate (3.02 meq/L) suggested a medium risk. Although, the concentration of toxic elements did not exceed the threshold limits; the long-term irrigation with mine water requires the monitoring of the molybdenum levels. These results suggest the potential for using artificial soils and mine water in post-mining land reclamation but highlight the need for the monitoring of their quality. Full article

Background/Objective: The endothelial glycocalyx is a gel-like layer on the vascular endothelial surface that is crucial for maintaining vascular homeostasis. Massive bleeding leads to the shedding of the glycocalyx, which can lead to vascular leakage during fluid administration. Recently, the beneficial effect of hydrogen inhalation in the treatment of hemorrhagic shock has been reported. However, the efficacy of hydrogen-rich saline in protecting the glycocalyx remains unclear. In this study, we investigated the effects of hydrogen-rich saline on glycocalyx degeneration. Methods: Rats under general anesthesia were divided into five groups: the sham, hemorrhagic shock, normal saline, colloid solution, and hydrogen-rich saline groups (n = 6 for each group). Blood was withdrawn, and blood pressure was maintained at 30–35 mmHg for 60 min. After inducing hemorrhagic shock in this way, each infusion product was administered intravenously to maintain blood pressure at 80 mmHg for 60 min. Glycocalyx thickness was assessed using the GlycoCheck system. Results: The use of hydrogen-rich saline significantly improved the survival rate (p < 0.05), and glycocalyx degeneration was significantly suppressed (p < 0.001), indicating the protective effect of hydrogen on the glycocalyx. Conclusion: Intravenous administration of hydrogen-rich saline in hemorrhagic shock attenuates glycocalyx degeneration compared to conventional fluid resuscitation, which can improve survival rates. Full article

In electrospun scaffolds, coaxial electrospinning is gaining increased attention due to its potential for biocomponent encapsulation and controlled delivery. However, the encapsulation of biocomponents, such as liposomes, remains challenging because of their low stability in commonly used electrospinning solvents. This study, therefore, aims to develop a novel coaxial electrospinning formulation for crafting a liposome-encapsulated, rapid-release coaxial fiber. Liposomes demonstrated desirable stability in fish gelatin/phosphate-buffered saline (PBS) solutions, which remain liquid at room temperature and exhibit exceptional spinnability at concentrations exceeding 80 w/v% due to the reduction in surface tension. Fluorescent labelling examinations confirmed the successful encapsulation of liposomes within coaxial fibers electrospun from a 160 w/v% gelatin/PBS core and a 20 w/v% PCL/chloroform/N,N-dimethylformamide (DMF) shell. The gelatin/PBS core solution formed solid ends at the tips of the core-shell fiber post-spinning, while maintaining a liquid state within the shell, thereby enabling the encapsulation of liposomes within the PCL coaxial fiber. Upon exposure to medium, the solid ends dissolve, enabling the rapid release of liposomes. The successful development of this liposome-loaded electrospun coaxial fiber, using fish gelatin, highlights its potential for creating advanced liposome delivery systems. Full article

The physiological functions of newborn calves are undeveloped, especially the immune system, making them susceptible to infections. In recent years, the theory of trained immunity has attracted attention and provided new strategies to prevent unknown infections in animals. This study investigated the effects of feeding yeast β-glucan on the intestinal and respiratory health of calves during the suckling period. Newborn Holstein calves (average birth weight: 36.18 ± 0.61 kg, mean ± SE) were randomly assigned to two groups: the PO (Per Os) group (n = 22) and the CON (Control) group (n = 22). Calves in the PO group were fed a yeast β-glucan solution (0.1 g/mL, 65 mg/kg body weight) at 3 and 6 days of age, respectively, while calves in the CON group received equal volumes of sterile saline orally at the same time. Blood and fecal samples were collected at 7 and 30 days of age, respectively. The results showed that (1) Compared to the CON group, being fed yeast β-glucan resulted in an inflammatory response after 24 h of the second administration, including increased gene expression of interleukin-6 (IL-6, p < 0.01), interleukin-1 beta (IL-1β, p < 0.01), and malonaldehyde (MDA, p < 0.001) content. Also, stimulation with β-glucan increased the concentrations of secreted immunoglobulin A (sIgA, p < 0.01) and defensins (p < 0.05) in the rectal feces. (2) Pre-stimulation with yeast β-glucan effectively reduced the incidence of diarrhea (p < 0.05) and bovine respiratory disease (BRD, p < 0.05) from day 31 to day 60. (3) At 30 days of age, the pre-stimulated calves had significantly lower serum DAO (p < 0.001) and MDA levels (p < 0.05), while they had higher levels of serum IL-6 (p < 0.01) and fecal slgA (p < 0.05) than calves in the CON group. (4) Pre-stimulation with yeast β-glucan altered the intestinal bacterial community; the Beta diversity results showed that the CON group and the PO group were clustered separately in the principal coordinate analysis (PCoA) graph. Obviously, the PO group sample points were more clustered. In conclusion, this study highlights the potential of yeast β-glucan-induced trained immunity to improve calf health during the suckling period. The findings offer new insights into the prevention of intestinal and respiratory infections in calves. Full article

Water scarcity has prompted researchers to intensify studies on the optimal use of saline water in irrigating agricultural crops to improve the efficiency of exploiting available water resources. Therefore, this study aimed to use selected spectral vegetation indices to investigate the potential of grafting in mitigating the effect of salinity on the growth of tomato plants grown under a hydroponic system. Three commercial tomato cultivars (Forester-F1, Feisty-Red, and Ghandowra-F1,) and five tomato hybrid rootstocks (Beaufort, Maxifort, Dynafort, Unifort, and Vivifort) were investigated using nutrient solutions at three salinity levels, namely, 2.5 dS m⁻¹ (S1, low salinity level), 6.0 dS m⁻¹ (S2, medium salinity level), and 9.5 dS m⁻¹ (S3, high salinity level). The results showed that Ghandowra-F1 had the best growth performance under hydroponics compared to the other two varieties. The increase in the salinity of the nutrient solution negatively affected the vegetation growth of tomato plants. Low and medium salinity did not show any significant effect on the three tomato varieties, unlike high salinity, which showed a significant negative effect on the vegetative growth of the plant. Thus, it is possible to successfully grow tomatoes in hydroponics using nutrient solutions with a salt concentration of up to 6.0 dS m⁻¹. Although there was a slight improvement in the vegetative growth of grafted tomato plants, all the studied rootstocks showed no significant differences compared to non-grafted tomato plants. This study could greatly contribute to strategies targeting the improvement of tomato production in hydroponics. Full article

Long-term human residence on the Moon is an inevitable trend in lunar exploration, necessitating the development of Bioregenerative Life Support Systems (BLSSs). In BLSSs, plant cultivation serves as the core functional unit, requiring substantial amounts of cultivation substrates. Lunar soil has potential as a cultivation substrate, but its suitability for plant growth must be improved to meet life-support requirements. As a fine-grained, organics-free, in situ resource, lunar soil’s high compaction significantly restricts crops’ root access to oxygen, water, and nutrients. While the addition of organic solid waste—a byproduct of BLSSs—could alleviate compaction, issues such as salinization, incomplete decomposition, and the presence of pathogens pose risks to crop health. In this study, we introduced earthworms into wheat cultivation systems to gradually digest, transfer (as vermicompost), and mix solid waste with a lunar soil simulant substrate. We set five experimental groups: a positive control group using vermiculite (named as V) as the optimal growth substrate, a negative control group using pure lunar soil simulant (LS), and three treatment groups using lunar soil simulant with solid waste and 15 (LS+15ew), 30 (LS+30ew), and 45 (LS+45ew) earthworms added. Our results demonstrated significant improvements in both compaction (e.g., bulk density, hydraulic conductivity) and salinization (e.g., salinity, electrical conductivity), likely due to the improved soil aggregate structures, which increased the porosity and ion adsorption capacity of the soil. Additionally, the microbial community within the substrate shifted toward a cooperative pattern dominated by significantly enriched plant probiotics. Consequently, the cultivated wheat achieved approximately 80% of the growth parameters (including production) compared to the control group grown in vermiculite with nutrient solution (representing ideal cultivation conditions), indicating sufficient nutrient supply from the mineralized waste. We can conclude that the earthworms “complementarily” improved the lunar soil simulant and organic waste by addressing compaction and salinization, respectively, leading to comprehensive improvements in key parameters, including the microbial environment. This study proposes a conceptual framework for improving lunar soil for crop cultivation, and it innovatively introduces earthworms as a preliminary yet effective solution. These findings provide a feasible and inspiring foundation for future lunar agriculture. Full article