Fermentation, Volume 9, Issue 12
2023 December - 37 articles
Cover Story: Several emerging mosquito control technologies require mass releases of adult male mosquitoes. Brizzee et al. used the Cas-CLOVER system in combination with PiggyBac transposase to produce Saccharomyces cerevisiae strains with multiple integrated copies of a female-specific insecticidal shRNA expression cassette. Consumption of the engineered yeast killed female mosquito larvae during development, facilitating production of adult Culex male mosquitoes (shown on the cover, photo by Keshava Mysore). Successful piloting of kilogram-scale fermentations demonstrated that the yeast, which was subsequently heat-killed and dried, yielding a shelf-stable formulation, can be deployed globally to support mosquito mass-rearing facilities. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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