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Fermentation, Volume 9, Issue 12

2023 December - 37 articles

Cover Story: Several emerging mosquito control technologies require mass releases of adult male mosquitoes. Brizzee et al. used the Cas-CLOVER system in combination with PiggyBac transposase to produce Saccharomyces cerevisiae strains with multiple integrated copies of a female-specific insecticidal shRNA expression cassette. Consumption of the engineered yeast killed female mosquito larvae during development, facilitating production of adult Culex male mosquitoes (shown on the cover, photo by Keshava Mysore). Successful piloting of kilogram-scale fermentations demonstrated that the yeast, which was subsequently heat-killed and dried, yielding a shelf-stable formulation, can be deployed globally to support mosquito mass-rearing facilities. View this paper
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Articles (37)

  • Article
  • Open Access
2 Citations
4,101 Views
22 Pages

16 December 2023

Amino acids have relevance in biorefining as fermentation nutrients but also as valued coproducts obtainable from plant biomass. Soy flour was studied as a representative low-cost protein source requiring hydrolysis to free primary amino acids for ut...

  • Review
  • Open Access
14 Citations
11,842 Views
38 Pages

16 December 2023

The natural fermentation of antibiotics, along with semi-synthetic and synthetic approaches, is one of the most important methods for their production. The majority of the antibiotic market comes from the fermentation of high-yielding (HY) fungal str...

  • Article
  • Open Access
9 Citations
4,540 Views
13 Pages

15 December 2023

Succinic acid (SA), a C4 tricarboxylic acid cycle intermediate, is used as raw material for bulk chemicals and specialty chemicals, such as tetrahydrofuran and 1,4-butanediol, as well as also being used to synthesize the biodegradable biopolymers PBS...

  • Article
  • Open Access
10 Citations
4,857 Views
17 Pages

15 December 2023

We investigated the effects of the moisture content and silage starter preparation on the fermentation quality, nutritional value, and in vitro digestibility of waxy corn processing byproducts and rice bran (WRB) mixed silage and waxy corn processing...

  • Article
  • Open Access
5 Citations
4,350 Views
17 Pages

15 December 2023

Soy (tofu) whey is one of the two side-streams from tofu processing, which has been shown to be suitable for microbial growth. In this work, probiotic Bifidobacterium animalis subsp. lactis Bl-04 and B94 were used to ferment soy whey with differ...

  • Article
  • Open Access
8 Citations
2,864 Views
16 Pages

15 December 2023

This work focuses on the establishment of analytical and sensory differences between young wines obtained from the same grape must fermented using different strategies. The main purpose is to provide winemakers with objective criteria to help them to...

  • Article
  • Open Access
3 Citations
2,180 Views
18 Pages

Effects of Metal Chloride Salt Pretreatment and Additives on Enzymatic Hydrolysis of Poplar

  • Chenbiao Zhao,
  • Shenyi Mai,
  • Meishan Fan,
  • Jun Xie and
  • Hongdan Zhang

14 December 2023

Metal chloride salt pretreatment was performed to isolate and convert cellulose to glucose from poplar. A glucose yield of 82.0% ± 0.7 was achieved after 0.05 mol/L AlCl3 pretreatment conducted at 180 °C for 20 min, ascribing to the remova...

  • Article
  • Open Access
9 Citations
5,813 Views
16 Pages

Torulaspora delbrueckii Improves Organoleptic Properties and Promotes Malolactic Fermentation in Carbonic Maceration Wines

  • Candela Ruiz-de-Villa,
  • Jordi Gombau,
  • Montse Poblet,
  • Albert Bordons,
  • Joan Miquel Canals,
  • Fernando Zamora,
  • Cristina Reguant and
  • Nicolas Rozès

14 December 2023

This study investigates the impact of inoculating Torulaspora delbrueckii (Td) strains during the initial phase of carbonic maceration (CM) vinification, aiming to enhance the fermentative process and unique characteristics of CM wines. CM is a winem...

  • Review
  • Open Access
86 Citations
36,516 Views
21 Pages

Traditional and New Microorganisms in Lactic Acid Fermentation of Food

  • Barbara Sionek,
  • Aleksandra Szydłowska,
  • Kübra Küçükgöz and
  • Danuta Kołożyn-Krajewska

14 December 2023

Lactic acid fermentation is one of the oldest and most commonly used methods of bioconservation. This process is widely used for food preservation and also for a production technique that relies on the metabolism of lactic acid bacteria (LAB) to conv...

  • Article
  • Open Access
3 Citations
3,005 Views
14 Pages

Xylitol Production by Debaryomyces hansenii in Extracted Olive Pomace Dilute-Acid Hydrolysate

  • Ana Rita C. Morais,
  • Luís C. Duarte,
  • Pedro Lourenço,
  • Ivone Torrado,
  • Teresa Brás,
  • Luísa A. Neves and
  • Florbela Carvalheiro

14 December 2023

The extracted olive pomace (EOP) is an industrial lignocellulosic by-product of olive pomace oil extraction, currently mainly used for energy production through combustion. In this work, the hemicellulosic fraction of EOP was selectively hydrolyzed b...

  • Article
  • Open Access
5 Citations
4,932 Views
16 Pages

High Production of Nattokinase via Fed-Batch Fermentation of the γ-PGA-Deficient Strain of Bacillus licheniformis

  • Xin Li,
  • Jing Yang,
  • Jun Liu,
  • Xiaohui Zhang,
  • Wei Wu,
  • Dazhong Yan,
  • Lihong Miao,
  • Dongbo Cai,
  • Xin Ma and
  • Shouwen Chen

13 December 2023

During the production of nattokinase (NK) by Bacillus species, certain by-products such as poly-γ-glutamic acid (γ-PGA) are simultaneously synthesized. The impact of γ-PGA synthesis on NK production remains unclear. In this study, w...

  • Article
  • Open Access
6 Citations
3,151 Views
12 Pages

Assessing the Impact of Simultaneous Co-Fermentation on Malolactic Bioconversion and the Quality of Cider Made with Low-Acidity Apples

  • Maria Luísa Cerri,
  • Tatiane Aparecida Gomes,
  • Matheus de Melo Carraro,
  • José Pedro Wojeicchowski,
  • Ivo Mottin Demiate,
  • Luiz Gustavo Lacerda,
  • Aline Alberti and
  • Alessandro Nogueira

13 December 2023

This study investigated the synergistic effects of combining Saccharomyces cerevisiae and Oenococcus oeni during the alcoholic fermentation of a low-acidity cider. The initial population of indigenous wild lactic acid bacteria (LAB) in the apple must...

  • Article
  • Open Access
5 Citations
2,733 Views
13 Pages

Immobilization of Phospholipase D for Production of Phosphatidylserine via Enzyme-Inorganic Hybrid Nanoflower Strategy

  • Shujing Zhang,
  • Hui Sun,
  • Zhiqi Huang,
  • Zhuoxuan Han,
  • Jiayi Hou,
  • Fuping Lu and
  • Yihan Liu

13 December 2023

Phosphatidylserine (PS) is a natural phospholipid with particular importance in the food, cosmetic, and pharmaceutical industries. Recently, the synthesis of PS mediated by phospholipase D (PLD) has drawn great attention. But the application of free...

  • Article
  • Open Access
3 Citations
4,202 Views
18 Pages

The Application of Adaptive Model Predictive Control for Fed-Batch Escherichia coli BL21 (DE3) Cultivation and Biosynthesis of Recombinant Proteins

  • Konstantins Dubencovs,
  • Arturs Suleiko,
  • Elina Sile,
  • Ivars Petrovskis,
  • Inara Akopjana,
  • Anastasija Suleiko,
  • Vytautas Galvanauskas,
  • Kaspars Tars and
  • Juris Vanags

12 December 2023

A model predictive control (MPC) method was investigated as a route to optimize and control the growth of E. coli BL21 (DE3) and biosynthesis of two different recombinant proteins (nerve growth factor NGF and coat protein of bacteriophage Qβ (Q&...

  • Article
  • Open Access
7 Citations
3,611 Views
25 Pages

Screening Bacterial Strains Capable of Producing 2,3-Butanediol: Process Optimization and High Diol Production by Klebsiella oxytoca FMCC-197

  • Anastasia Marina Palaiogeorgou,
  • Ermis Ioannis Michail Delopoulos,
  • Apostolis A. Koutinas and
  • Seraphim Papanikolaou

12 December 2023

In the present investigation, the potential of various newly isolated strains which belong to the Enterobacteriaceae family to produce 2,3-butanediol (BDO), an important bio-based compound, was studied. The most interesting strain, namely Klebsiella ...

  • Review
  • Open Access
16 Citations
8,974 Views
28 Pages

Oleaginous Yeast Biorefinery: Feedstocks, Processes, Techniques, Bioproducts

  • Teresa Lopes da Silva,
  • Afonso Fontes,
  • Alberto Reis,
  • Carla Siva and
  • Francisco Gírio

12 December 2023

The world climate crisis has triggered the search for renewable energy sources. Oleaginous yeasts are a potential renewable source of biofuels. However, the yeast-derived biofuels cost is still non-competitive with the fossil fuel prices. To improve...

  • Article
  • Open Access
2 Citations
2,598 Views
17 Pages

Effect of Yeast Derivatives and β-Glucanases on Ageing over Lees Process of Tempranillo Red Sparkling Wine

  • Raúl Moyano-Gracia,
  • Josefina Vila-Crespo,
  • Violeta Ruipérez,
  • José Manuel Rodríguez-Nogales and
  • Encarnación Fernández-Fernández

12 December 2023

This study focuses on improving the second fermentation and the in-bottle ageing over lees process for 9 and 21 months of a red sparkling wine. The aim of the study was to enhance wine quality and try to make it more pleasant for consumers. For this...

  • Article
  • Open Access
3 Citations
2,448 Views
13 Pages

The cultivation and overall quality of Litchi, a fruit of significant commercial value in China, are hindered by the presence of the oomycetes pathogen Peronophythora litchii. This pathogen is responsible for the occurrence of litchi downy blight, re...

  • Article
  • Open Access
6 Citations
3,793 Views
13 Pages

Sediment Microbial Fuel Cells with Algae-Assisted Cathodes for Electricity Generation and Bio-Treatment of Sewage Sludge

  • Lizheng Chen,
  • Hongyi Zhang,
  • Yongqi Li,
  • Chunxia Zhao,
  • Ling Liu,
  • Lipin Li,
  • Li Sun and
  • Hui Li

In this study, an algal–bacterial symbiotic consortium was integrated with the sediment microbial fuel cell (SMFC) to construct an algal–bacterial cathode SMFC (AC-SMFC) for excess sewage sludge treatment and electricity generation. A bac...

  • Article
  • Open Access
4 Citations
3,046 Views
17 Pages

With the addition of Tween 80, potassium hydroxide pretreatment and enzymatic hydrolysis were statistically optimized to maximize sugar yields from corn cob (CC). The results indicated that the sugar yields from CC could be influenced significantly b...

  • Article
  • Open Access
5 Citations
2,984 Views
12 Pages

Brewers’ spent grain (BSG) constitutes the primary by-product of the brewing industry. The valorization of BSG from a circular economy perspective is of high industrial interest. The objective of this study was the exploitation of BSG for the m...

  • Article
  • Open Access
4 Citations
2,554 Views
20 Pages

A continuing interest of scientists regarding the development of new β-carotene production technologies is due to the high biological activity of this compound and its wide application range. Bacteria are considered among the possible β-car...

  • Article
  • Open Access
1 Citations
2,483 Views
17 Pages

Microbial Diversity of Traditional Livno Cheese from Bosnia and Herzegovina

  • Tarik Dizdarević,
  • Svijetlana Sakić-Dizdarević,
  • Davide Porcellato,
  • Zlatan Sarić,
  • Mersiha Alkić-Subašić,
  • Roger K. Abrahamsen and
  • Judith A. Narvhus

Traditional dairy products, especially cheeses, represent part of the cultural food heritage of many countries. In addition, these cheeses constitute microbiological “reservoirs”, of which many have been lost due to the introduction of th...

  • Article
  • Open Access
5 Citations
3,202 Views
20 Pages

Effect of Mixed Lactiplantibacillus plantarum- and Bacillus subtilis-Fermented Feed on Growth, Immunity, and Intestinal Health of Weaner Pigs

  • Jun Chen,
  • Guofang Wu,
  • Huili Pang,
  • Jiyun Hua,
  • Yifei Guan,
  • Miao Zhang,
  • Yaoke Duan,
  • Guangyong Qin,
  • Lei Wang and
  • Zhongfang Tan
  • + 1 author

Bamei pigs, an eximious local breed reared on the Tibetan Plateau of China, are facing problems such as feed shortages, weaning stress, and antibiotic abuse. This study aimed to improve the quality of feed, growth performance, intestinal microbiota,...

  • Article
  • Open Access
6 Citations
4,724 Views
13 Pages

29 November 2023

Recently, the extensive spread of some algae along coastlines has surged into unmanageable thick decomposition layers. This study aimed to demonstrate the use of Sargassum horneri as a biomass resource for ethanol production through the continuous hy...

  • Article
  • Open Access
9 Citations
5,104 Views
16 Pages

29 November 2023

High production cost is one of the major factors that limit the market growth of polyhydroxyalkanoates (PHAs) as a biopolymer. Improving PHA synthesis performance and utilizing low-grade feedstocks are two logical strategies for reducing costs. As an...

  • Article
  • Open Access
1 Citations
6,342 Views
17 Pages

Optimization of a Bacterial Cultivation Medium via a Design-of-Experiment Approach in a Sartorius Ambr® 15 Fermentation Microbioreactor System

  • Antonio Baccante,
  • Pasquale Petruccelli,
  • Giovanni Saudino,
  • Elena Ragnoni,
  • Erik Johansson,
  • Vito Di Cioccio and
  • Kleanthis Mazarakis

27 November 2023

In the evolving landscape of sustainable biopharmaceutical process development, the utilization of bacteria in the production of various compounds via fermentation has attracted extensive attention from scientists. A successful fermentation process a...

  • Article
  • Open Access
3 Citations
2,930 Views
16 Pages

27 November 2023

Trans-2-decenoic acid has a wide range of applications, including those in medicine, food, and health care. Therefore, the industrial production of trans-2-decenoic acid is particularly important. However, few studies have focused on medium-chain uns...

  • Review
  • Open Access
1 Citations
4,557 Views
14 Pages

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

  • Wenhu Chen,
  • Qian Liu,
  • Meng Liu,
  • Hongling Liu,
  • Di Huang,
  • Yi Jiang,
  • Tengfei Wang and
  • Haibo Yuan

25 November 2023

Vitamin C, a water-soluble vitamin with strong reducing power, cannot be synthesized by the human body and participates in a variety of important biochemical reactions. Vitamin C is widely used in the pharmaceutical, food, health care, beverage, cosm...

  • Article
  • Open Access
3,099 Views
13 Pages

Generation of a Culex Male Mosquito Sex-Separation RNAi Yeast Strain Using Cas-CLOVER and Super PiggyBac Engineering in Saccharomyces cerevisiae

  • Corey Brizzee,
  • Keshava Mysore,
  • Teresia M. Njoroge,
  • Seth McConnell,
  • Jack Crawford and
  • Molly Duman-Scheel

Several emerging mosquito control technologies require mass releases of adult male mosquitoes. Previous studies resulted in the generation of a laboratory female-specific larvicidal yeast strain targeting the GGT gene, which facilitated the laborator...

  • Article
  • Open Access
2 Citations
5,280 Views
10 Pages

Testosterone Biosynthesis from 4-Androstene-3,17-Dione Catalyzed via Bifunctional Ketoreductase

  • Yi Wei,
  • Guangyao Mei,
  • Jinlin Zhao,
  • Shaoyang Zhang,
  • Wenping Qin,
  • Qing Sheng and
  • Zhongyi Yang

Testosterone (TS) is an important androgen drug and a precursor of steroid drug synthesis. Ketoreductase 2 (KR-2) (GenBank accession no. ABP64403.1) is observed to stereo-selectively catalyze the bioreduction of 4-androstene-3,17-dione (4-AD) to test...

  • Article
  • Open Access
5 Citations
3,036 Views
19 Pages

The clinical antibiotic gentamicin is a mixture of several difficult-to-separate components, the minor group of which is gentamicin C1a, a precursor for the synthesis of the high-efficacy and low-toxicity antibiotic etimicin. This study aimed to achi...

  • Article
  • Open Access
4 Citations
3,253 Views
18 Pages

Whole-Genome Sequencing of Lactiplantibacillus plantarum YY-112 and Investigation of Its Immune-Modulating Abilities In Vivo

  • Mengfan Luo,
  • Wanyi Zhou,
  • Wenyang Tao,
  • Jianrong Xing,
  • Jingrui Li,
  • Ying Yang and
  • Yuxing Guo

A potentially novel probiotic strain, YY-112, was previously isolated and identified as Lactiplantibacillus pentosus using 16S rDNA sequencing. The whole genome analysis showed that strain YY-112 has the potential to metabolize carbohydrates in the g...

  • Article
  • Open Access
5 Citations
3,488 Views
16 Pages

The commercial potential of Trametes versicolor laccases in the degradation of various persistent contaminants is significant. Despite numerous attempts through rational metabolic engineering to enhance the properties of laccases, the outcomes have p...

  • Article
  • Open Access
3 Citations
2,509 Views
11 Pages

Effect of Yogurt Acid Whey on the Quality of Maize Silage

  • Irida Palamidi,
  • Vasileios V. Paraskeuas,
  • Basiliki Kotsampasi,
  • Ioannis Hadjigeorgiou,
  • Ioannis Politis and
  • Konstantinos C. Mountzouris

The increasing popularity of Greek yogurt generates large amounts of acid whey worldwide. The use of yogurt acid whey in animal nutrition is limited. The aim of this study was to determine the effect of a yogurt acid whey powder (YAWP) addition to ma...

  • Article
  • Open Access
6 Citations
4,370 Views
14 Pages

Anaerobic Co-Digestion of Cattle Manure and Brewer’s Residual Yeast: Process Stability and Methane and Hydrogen Sulfide Production

  • Luana Alves Akamine,
  • Roberta Passini,
  • João Antônio Silva Sousa,
  • Aline Fernandes and
  • Maria Joselma de Moraes

Anaerobic co-digestion (AcoD) of animal waste and agro-industrial by-products has been widely studied and employed to increase biogas production potential and enhance process stability. This study evaluated the AcoD of cattle manure (CM) and brewer’s...

  • Article
  • Open Access
10 Citations
2,550 Views
14 Pages

Valorization of Macauba (Acromia aculeata) for Integrated Production of Lipase by Yarrowia lipolytica and Biodiesel Esters

  • Filipe Smith Buarque,
  • Marcelle A. Farias,
  • Júlio Cesar Soares Sales,
  • Adriano Carniel,
  • Bernardo Dias Ribeiro,
  • Verônica Regina de Oliveira Lopes,
  • Aline Machado Castro and
  • Maria Alice Zarur Coelho

Enzymatic biodiesel production is a potential eco-friendly alternative to the conventional chemical route which requires extensive study to reduce the costs associated with the application of commercial enzymes. Thus, this study aimed to develop a bi...

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Fermentation - ISSN 2311-5637