Special Issue "Advances in Liquid Separation Techniques for Food and Pharmaceutical Analysis"
Deadline for manuscript submissions: 31 August 2018
Today, the demand for highly efficient, selective, sensitive, reproducible and green analytical methodologies for complex sample preparation, fractionation and/or extraction of bioactive compounds, analysis and chemical characterization, requires continuous advances in separation media, adsorbent materials and detection techniques. All these aspects belong to analytical methodologies where liquid separation techniques play the major role.
The main aims of the present Special Issue on "Advances in Liquid Separation Techniques for Food and Pharmaceutical Analysis" belong to both fundamental studies and real case multidisciplinary investigations. Contributions to this issue, both in the form of original research or review articles, have the broad scope to illustrate recent and future trends of liquid chromatography and hyphenated techniques offering a clear vision and insights for new methodologies, improving instrumental performances and providing sensitivity and specificity.
Innovative approaches in the field of pharmaceutical sciences, cosmetics, nutraceutics, food chemistry, finalized to the extraction, characterization, purification and validation of chiral or achiral active pharmaceutical ingredients, and natural bioactive compounds from plants, are particularly welcome. Also of interest would be studies concerning molecules that are obtained from waste and re-used as secondary raw materials. Studies that involve UPLC, SFC and their hyphenation with mass spectrometry are also strongly encouraged.
Dr. Nicola Marchetti
Dr. Anna Laura Capriotti
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- HPLC-, UPLC-, SFC-MS
- Sample preparation
- Food bioactive compounds
- Active pharmaceutical ingredients
- Purification/extraction techniques
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: The way to ultrafast, high-throughput enantioseparations of bioactive compounds in liquid and supercritical fluid chromatography
Article type: Review
Author: Prof. Alberto Cavazzini （University of Ferrara, Italy）
Abstract：Until less than 10 years ago, chiral separations were carried out with columns packed with 5 of 3 μm fully porous particles (FPPs). Times required to risolve enantiomeric mixtures were routinely larger than 30 minutes or so. Pushed especially by stringent requirements from medicinal and pharmaceutical industries, during the last years the field of chiral separations by liquid chromatography has undergone a true revolution.
With the purpose of developing ever faster and efficient method of separations, indeed, very efficient particle formats, such as superficially porous particles (SPPs) or sub-2μm FPPs, have been functionalized with chiral selectors and employed in ultrafast applications. Thanks to the use of short column (1-2 cm long), packed with these new generation chiral stationary phases (CSPs), operated at very high flow rates (5-8 mL/min), resolution of racemates could be accomplished in very short time, in many cases smaller than 1 sec. in normal-, reversed-phase and HILIC conditions.
Additionally, these CSPs have been found to be extremely promising also to carry out high-throughput separations under supercritical fluid chromatography (SFC) conditions.
In this manuscript, we will review the most important results that have been recently achieved in terms of ultrafast, high-throughput enantioseparations both in liquid and supercritical fluid chromatography with particular attention to the very important field of bioactive chiral compounds. Attention will be focused not only on the latest introduced CSPs and their applications, but also on instrumental modifications which are required in some cases in order to fully exploit the intrinsic potential of new generation chiral columns.
Title: Variation in the carotenoid composition and in their bioaccessibility from Delica (Cucurbita maxima) and Violina (Cucurbita moschata) pumpkins with food processing
Article type: Article
Author: Prof. Dr. Nicola Marchetti（University of Ferrara, Italy）
Abstract：In the last years, a great attention has been dedicated to the consumption of fresh vegetables and fruits since they have antioxidant and anticarcinogenic properties. Carotenoids are a widespread group of fat soluble pigments and their major nutritional importance comes from their pro–vitamin A activity.
In this study, two different cultivars of pumpkin (Cucurbita maxima, also named ‘Delica’ and Cucurbita moschata, known as ‘Violina’) from southern Po Delta area were studied in terms of carotenoids content and the influence of food processing on compositional changes and carotenoids bioaccessibility.
Qualitative investigation and quantitative determination have been performed on sample extracts by C3o HPLC with UV-Vis detector. In agreement with literature studies on selectivity, two main classes of compounds can be separated: early eluted epoxy– and hydroxy–carotenoids; more retained hydrocarbon–derivatives or carotenes. Major qualitative differences are visible in the chromatographic profiles: C. maxima presented 10 well separated peaks while it was smaller for C. moschata (6 peaks). The identification of chromatographic bands was tentatively obtained by means of (i) the chromatographic parameters, (ii) the UV–Vis spectra and relative maxima intensities, (iii) MS/MS spectra. Quantification of target carotenoids was performed with external calibration through analytical standards. The concentration of lutein and β-carotene was higher in C. maxima than in the other variety C. moschata.
Carotenoids are susceptible to degradation (isomerization and oxidation) during food processing (i.e., cooking) and the concentration of lutein and β-carotene were monitored in oven–cooked and steam– cooked pumpkins. Steam cooking process resulted to be superior to the other in terms of limiting the loss of carotenoids. A complete functional profile of pumpkins as a source of carotenoids was gained with the evaluation of their in vitro bioaccessibility and their bioavailability after intake during human digestion. Bioaccessibility of lutein and β-carotene were estimated by in vitro static digestion model that involves salivary, gastric and duodenal phases. Bioaccessibility values progressively increase from salivary to duodenal phase for both pumpkin variety and cooking method. At the duodenal stage bioaccessibility of lutein was always three times lower than β-carotene and no specific differences in the bioaccessibility were evidenced between the two cultivars for all the three digestion phases.
Keywords: carotenoids, pumpkins, HPLC – DAD, C30 column, bioaccessibility
Title: New Findings in Prunus padus l. Fruits as Source of Natural Compounds: Characterization of Metabolite Profiles and Antioxidant Activity
Article type: Article
Authors: Donno D., Mellano M.G., De Biaggi M., Riondato I., Rakotoniaina E.N., Beccaro G.L.
Affiliation: Università degli Studi di Torino, Italy
Abstract：In recent years, wild food plants have become very attractive to the food industry, prompting their use as replacements for synthetic chemicals and nutraceuticals, but neglected and underutilized natural food resources are suffering from less attention and research, and their nutritional, economic and socio-cultural potentials are not fully exploited. A global trend for the revival of interest in the use of traditional plants in daily menu has been observed. European bird cherry (Prunus padus L.) has been known since Middle Age for its medical/food use and high health-promoting value. This study aimed to assess the potential of these fruits as potential source of bioactive compounds by characterizing its physicochemical traits, nutraceutical properties, phytochemical composition by HPLC fingerprint, and antioxidant capacity. Fully ripened fruits (0.5 kg for each biological replication) of a cultivated genotype of Prunus padus L. (Colorata cv) were collected in the in mid-July 2017, in Chieri (45°1’0”N, 7°49’0”E, at 305 m A.S.L.), Piedmont (north-western Italy), in a germplasm repository of the Department of Agricultural, Forest and Food Sciences, University of Turin (voucher specimens were deposited). In this research the used methods allowed an accurate and rapid measurement of total monomeric anthocyanins and total polyphenolic content. The TPC (194.22±32.83 mgGAE/100gFW) and TAC (147.42±0.58 mgC3G/100gFW) values were obtained from the analyzed extracts. The most important phytochemical class was organic acids (48.62±2.31%), followed by polyphenols (35.34±1.80%), monoterpenes (9.36±0.64%), and vitamin C (6.68±0.22%). In this research the most important flavonols selected as marker were quercitrin (16.37±3.51 mg/100 gFW) and quercetin (11.86 ±2.36 mg/100 gFW), followed by hyperoside (7.38±0.41 mg/100 gFW) and rutin (2.67 ±1.02 mg/100 gFW). Moreover, the presence of cinnamic acids, in particular chlorogenic acid (10.48±0.28 mg/100 gFW), may be important when fruits are processed, as these compounds are considered a preferential substrate for the catecholase activity of polyphenol oxidase. Bird cherry fruits showed a total vitamin C content (76.07±13.34 mg/100 gFW) similar to the most common fruits, as kiwifruit (74.56±9.84 mg/100 gFW) and orange (71.12±1.96 mg/100 gFW). Data were reported based on fresh weight. Moreover, fresh fruits showed a mean antioxidant activity value of 17.78±0.84 mmol Fe2+•kg−1. Despite the seeds and leaves contain cyanogenic glycosides, this study showed that these fruits could be a potential natural source of bioactive compounds with high antioxidant properties, due to the contents of organic and phenolic acids, catechins, and a synergetic effect of vitamin C and flavonoids.
Keywords: bird cherry, fruit healthy properties, biodiversity, phytochemicals, traditional food, chromatographic fingerprinting, antioxidants
Title: Chromatographic strategies for separation of bioactive compounds in food matrices
Article type: Review
Author: Prof. Dr. Anna Laura Capriotti (University of Rome, Italy)
Title: Tracing the Thermal History of Seafood Products Through Lysophospholipid Analysis by Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization and Fourier-Transform Mass Spectrometry
Article type: Article
Authors: Dr. Ilario Losito, Dr. Laura Facchini, Dr. Rosa Catucci and Prof. Tommaso Cataldi and Francesco Palmisano (University of Bari "Aldo Moro", Italy)
Title: A toolbox of methods for characterization of phospholipids and their oxidized derivatives in food and biological samples
Article type: Review
Authors: Dr. Agnieszka Bartoszek
Title: ODAP content in Lathyrus sativus (grass pea): implementation and validation of a simple and fast LC-MS/MS method
Article type: Article
Authors: Dr. Andreia Bento da Silva (Food Functionality and Bioactives Lab, Portugal)
Title: Chiral Separation of Indapamide on Polysaccharide Stationary Phase
Article type: Article