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  • Article
  • Open Access
12 Citations
3,968 Views
17 Pages

The principal objective of this study was to isolate and identify the microorganisms present in commercial kefir grains, a novel kefir-fermented coconut water (CWK) and a novel coconut water kefir-fermented sourdough using phenotypic identification a...

(This article belongs to the Special Issue Microbial Safety and Biotechnology in Food Production and Processing)
  • Article
  • Open Access
4 Citations
4,483 Views
16 Pages

Functional Properties of Microorganisms Isolated from Formulated Sourdough, Coconut Water Kefir, and Kefir

  • Mansi Jayantikumar Limbad,
  • Noemi Gutierrez Maddox,
  • Nazimah Hamid and
  • Kevin Kantono

Recently, there has been a renewed interest in the fermentation of kefir grains using fruit-based substrates, such as coconut water. Kefir grains contain a mixture of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeast, which have impor...

(This article belongs to the Special Issue Application of Lactic Acid Bacteria in Fermented Food)
  • Article
  • Open Access
10 Citations
8,239 Views
28 Pages

Synergistic Bioactive Potential of Combined Fermented Kombucha and Water Kefir

  • Chiara La Torre,
  • Roberta Pino,
  • Alessia Fazio,
  • Pierluigi Plastina and
  • Monica Rosa Loizzo

The rising interest in functional fermented beverages, such as kombucha and water kefir, has stimulated research into their health benefits. This study aimed to investigate the combined bioactive potential of kombucha and water kefir by fermenting a...

  • Article
  • Open Access
4 Citations
1,712 Views
28 Pages

Production and Characterization of Kefir Beverages by Fermentation of Whole Milk with Milk or Water Kefir Grains

  • Eduardo Balvis Outeiriño,
  • Marta Abajo Justel,
  • Cristina Pérez Novo,
  • Alberto Acuña Couñago and
  • Nelson Pérez Guerra

7 May 2026

This study evaluated the fermentation kinetics and properties of kefir beverages produced from whole milk using milk kefir grains (MKGs) or water kefir grains (WKGs) over 48 h. MKGs showed higher initial microbial loads and promoted rapid acidificati...

(This article belongs to the Special Issue Physicochemical Properties and Functional Performance of Dairy Products)
  • Review
  • Open Access
2 Citations
3,910 Views
17 Pages

Potential Characteristics of Water Kefir on Health Benefits

  • Alejandra Yerovi-López and
  • Amparo Gamero

Water kefir is a non-dairy fermented beverage that has received increasing scientific attention due to its complex microbial composition and its suitability for individuals with dietary restrictions. This review critically examines the current body o...

(This article belongs to the Section Tea, Coffee, Water, and Other Non-Alcoholic Beverages)
  • Article
  • Open Access
48 Citations
19,007 Views
16 Pages

The aim of the present study was to investigate the feasibly of using traditional milk kefir grains for the production of water kefir-like beverages and assess the changes in the physicochemical characteristics and the microbial populations of the fe...

(This article belongs to the Special Issue Fermented Foods: Traditional Processes, Innovative Strategies, and Challenges)
  • Article
  • Open Access
7 Citations
6,415 Views
9 Pages

The Impact of Kefir on Epidermal Water Homeostasis in Healthy Human Skin

  • Emília Alves,
  • João Gregório,
  • Patrícia Rijo,
  • Catarina Rosado and
  • Luís Monteiro Rodrigues

19 July 2022

Kefir, a symbiotic consortium of diverse bacteria and yeasts, is one of the most popular probiotic foods on the market. Its consumption has been referred to as beneficial in human skin health, namely in the reinforcement of skin’s barrier funct...

(This article belongs to the Special Issue Skin Homeostasis: Mechanisms, Communication Routes, and Imbalances)
  • Article
  • Open Access
33 Citations
5,486 Views
18 Pages

Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms

  • Mariana Gonda,
  • Gabriela Garmendia,
  • Caterina Rufo,
  • Ángela León Peláez and ,
  • Michael Wisniewski,
  • Samir Droby and
  • Silvana Vero
  • + 2 authors+ 0 author

The capacity of microorganisms from water kefir (WK) to control Aspergillus flavus growth during the aerobic phase of ensiled sorghum grains was determined. Sorghum inoculated with A. flavus was treated with filter-sterilized and non-sterilized water...

(This article belongs to the Special Issue Non-conventional Yeasts: Genomics and Biotechnology)
  • Article
  • Open Access
43 Citations
9,967 Views
15 Pages

Influence of Substrate on the Fermentation Characteristics and Culture-Dependent Microbial Composition of Water Kefir

  • Emanuele Zannini,
  • Kieran M. Lynch,
  • Laura Nyhan,
  • Aylin W. Sahin and ,
  • Patrick O’ Riordan,
  • Daenen Luk and
  • Elke K. Arendt
  • + 2 authors+ 0 author

Water kefir is a sparkling fermented beverage produced by fermenting water kefir grains in a sucrose solution containing dried fruits or fruit extracts. The objective of this study was to investigate the influence of substrate composition on the ferm...

(This article belongs to the Special Issue Research Advances in Fermented Beverages)
  • Feature Paper
  • Review
  • Open Access
23 Citations
25,723 Views
13 Pages

Water Kefir: Review of Microbial Diversity, Potential Health Benefits, and Fermentation Process

  • Klinger Vinícius de Almeida,
  • Cíntia Tomaz Sant’ Ana,
  • Samarha Pacheco Wichello,
  • Gabriele Estofeles Louzada and ,
  • Silvani Verruck and
  • Luciano José Quintão Teixeira
  • + 1 author+ 0 author

17 March 2025

Water kefir is a non-dairy fermented beverage that ferments water kefir grains in a sucrose solution. These grains harbor a diverse microbiota, including lactic acid bacteria, acetic acid bacteria, and yeast species. The composition of water kefir is...

(This article belongs to the Special Issue Feature Reviews in Food Processing Technologies: Present Innovations and Future Opportunities)
  • Article
  • Open Access
7 Citations
3,114 Views
12 Pages

Modulation of the Toll-like Receptor 3-Mediated Intestinal Immune Response by Water Kefir

  • Stefania Dentice Maidana,
  • Ramiro Ortiz Moyano,
  • Mariano Elean,
  • Yoshiya Imamura and ,
  • Leonardo Albarracín,
  • Fu Namai,
  • Yoshihito Suda,
  • Keita Nishiyama,
  • Julio Villena and
  • Haruki Kitazawa
  • + 5 authors+ 0 author

Kefir has been associated with beneficial effects on its host’s health. The previous works examining the impact of kefir on the immune system focused on milk kefir or the exopolysaccharides and bacterial strains derived from it, while water kef...

  • Article
  • Open Access
24 Citations
9,364 Views
17 Pages

Microbial and Chemical Changes during Fermentation of Coconut Water Kefir Beverage

  • Mansi Limbad,
  • Noemi Gutierrez-Maddox,
  • Nazimah Hamid,
  • Kevin Kantono and ,
  • Tingting Liu and
  • Tim Young
  • + 1 author+ 0 author

18 June 2023

This study investigated changes in the microbial growth and chemical characteristics of coconut water kefir (CWK) during fermentation. The carbohydrate profile, in terms of glucose and sucrose consumption, production of carboxylic acids, and changes...

(This article belongs to the Section Food Science and Technology)
  • Article
  • Open Access
334 Views
21 Pages

Characterization and Evaluation of Apple Pomace Extract as a Fermentable Substrate in Water Kefir

  • Pável Padilla-Tamayo,
  • Armando Quintero-Ramos,
  • Miguel Ángel Sánchez-Madrigal,
  • José Carlos Espinoza-Hicks and ,
  • Ever Hernández-Olivas,
  • Iván Salmerón,
  • José Rafael Linares-Morales,
  • María del Cielo Valdez-Cárdenas and
  • Martha Graciela Ruiz-Gutiérrez
  • + 4 authors+ 0 author

Water kefir is a fermented beverage produced by a consortium of diverse microorganisms that can adapt to different substrate sources, modifying the beverage’s physicochemical and functional properties. Apple pomace is an agro-industrial by-prod...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
7 Citations
5,151 Views
13 Pages

The Production of Water Kefir Drink with the Addition of Dried Figs in the Horizontal Rotating Tubular Bioreactor

  • Mladen Pavlečić,
  • Mario Novak,
  • Antonija Trontel,
  • Nenad Marđetko and ,
  • Vlatka Petravić Tominac,
  • Ana Dobrinčić,
  • Monika Kralj and
  • Božidar Šantek
  • + 3 authors+ 0 author

6 September 2024

Water kefir is a product obtained through the fermentation of sucrose solution, usually with some kind of dried fruit addition, by a combined culture of micro-organisms which are contained within kefir grains. Its popularity is rising because of the...

(This article belongs to the Section Food Engineering and Technology)
  • Article
  • Open Access
20 Citations
5,857 Views
29 Pages

Antioxidant, Antithrombotic and Anti-Inflammatory Properties of Amphiphilic Bioactives from Water Kefir Grains and Its Apple Pomace-Based Fermented Beverage

  • Dimitra Papadopoulou,
  • Vasiliki Chrysikopoulou,
  • Aikaterini Rampaouni,
  • Christos Plakidis and ,
  • Anna Ofrydopoulou,
  • Katie Shiels,
  • Sushanta Kumar Saha and
  • Alexandros Tsoupras
  • + 3 authors+ 0 author

29 January 2025

Kefir-based fermentation products exhibit antioxidant and anti-inflammatory effects against oxidative stress, inflammation, platelet activation and aggregation, and other related manifestations, thereby preventing the onset and development of several...

(This article belongs to the Special Issue Antioxidants from Sustainable Food Sources)
  • Article
  • Open Access
11 Citations
6,153 Views
23 Pages

5 February 2025

Today, the global trend toward plant-based beverages has grown for sustainability, health-related, lifestyle, and dietary reasons. Among them, drinks produced from almonds have been recognized as a concentrated nutrient source. Commercial almond milk...

(This article belongs to the Section Food Chemistry)
  • Article
  • Open Access
1 Citations
1,633 Views
23 Pages

Preliminary Evaluation of the Gut Microbiota Modulatory Potential of Malaysian Kefir Water in Ageing Mice

  • Muganti Rajah Kumar,
  • Aaron Opoku Amankwaa,
  • Nurulain Syahirah Razali,
  • Nurul Elyani Mohamad and ,
  • Melati Khalid,
  • Janna Ong Abdullah,
  • Mas Jaffri Masarudin,
  • Mohd. Azuraidi Osman,
  • Nik Mohd Afizan Nik Abd Rahman and
  • Noorjahan Banu Alitheen
  • + 5 authors+ 0 author

11 November 2025

Ageing is often accompanied by gut microbiota alterations that contribute to dysbiosis—a recognised hallmark of ageing and a risk factor for neurodegenerative diseases. Probiotic interventions offer a promising approach to restore microbial hom...

(This article belongs to the Special Issue The Regulation of Gut Microbiota by Probiotics and Dietary Nutrients in Foods to Improve Human Health)
  • Article
  • Open Access
11 Citations
7,799 Views
15 Pages

The Impact of Production Techniques on the Physicochemical Properties, Microbiological, and Consumer’s Acceptance of Milk and Water Kefir Grain-Based Beverages

  • Martina Arapović,
  • Leona Puljić,
  • Nikolina Kajić,
  • Marija Banožić and ,
  • Brankica Kartalović,
  • Kristina Habschied and
  • Krešimir Mastanjević
  • + 2 authors+ 0 author

The increasing focus on a healthy lifestyle has emphasized a connection between gut microbiome and health. Consumers face the choice between consuming traditional dairy kefirs or more trendy fruit-based fermented drinks. Also, the aim of this study i...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
11 Citations
3,858 Views
16 Pages

Modulation of the Gut–Lung Axis by Water Kefir and Kefiran and Their Impact on Toll-like Receptor 3-Mediated Respiratory Immunity

  • Stefania Dentice Maidana,
  • Julio Nicolás Argañaraz Aybar,
  • Leonardo Albarracin,
  • Yoshiya Imamura and ,
  • Luciano Arellano-Arriagada,
  • Fu Namai,
  • Yoshihito Suda,
  • Keita Nishiyama,
  • Julio Villena and
  • Haruki Kitazawa
  • + 5 authors+ 0 author

17 November 2024

The beneficial effect of milk kefir on respiratory heath has been previously demonstrated; however, water kefir and kefiran in the context of respiratory viral infections have not been investigated. Water kefir and kefiran could be alternatives to mi...

(This article belongs to the Section Natural and Bio-derived Molecules)
  • Article
  • Open Access
21 Citations
5,424 Views
16 Pages

29 April 2023

Water kefir grains cannot grow in milk. Therefore, the aim of this study was to investigate whether water kefir grains can show activity in demineralized whey, an environment containing lactose as a carbon source. The physicochemical, microbiological...

(This article belongs to the Special Issue Recent Advances in Cheese and Fermented Milk Production)
  • Article
  • Open Access
17 Citations
3,351 Views
17 Pages

Multi-Component Biodegradable Materials Based on Water Kefir Grains and Yeast Biomasses: Effect of the Mixing Ratio on the Properties of the Films

  • Agustina Lago,
  • Juan F. Delgado,
  • Guillermo D. Rezzani,
  • Celeste Cottet and ,
  • Yuly A. Ramírez Tapias,
  • Mercedes A. Peltzer and
  • Andrés G. Salvay
  • + 2 authors+ 0 author

7 June 2023

The use of biopolymeric materials is restricted for some applications due to their deficient properties in comparison to synthetic polymers. Blending different biopolymers is an alternative approach to overcome these limitations. In this study, we de...

(This article belongs to the Section Biobased and Biodegradable Polymers)
  • Article
  • Open Access
14 Citations
5,903 Views
16 Pages

Microbial Composition of Water Kefir Grains and Their Application for the Detoxification of Aflatoxin B1

  • Weidong Ouyang,
  • Zhenlin Liao,
  • Ximiao Yang,
  • Xiao Zhang and ,
  • Xiaoxuan Zhu,
  • Qingping Zhong,
  • Li Wang,
  • Xiang Fang and
  • Jie Wang
  • + 4 authors+ 0 author

15 February 2024

Water kefir grains (WKGs), the starter used to develop a traditional beverage named water kefir, consist of a symbiotic mixture of probiotics with diverse bioactivities, but little is known about their abilities to remove mycotoxins that have serious...

(This article belongs to the Section Mycotoxins)
  • Article
  • Open Access
3 Citations
1,059 Views
21 Pages

Formation Mechanism of Key Flavor Compounds During the Fermentation of Strawberry Juice with Water Kefir Grains

  • Linlin Yin,
  • Shunchang Pu,
  • Qianqian Tong,
  • Zhina Chen and ,
  • Tao Ye and
  • Shoubao Yan
  • + 1 author+ 0 author

10 April 2026

Water kefir grains are complex probiotic granules that can efficiently ferment fruit and vegetable juices and significantly improve product flavor. However, the mechanisms of flavor formation remain unclear, which limits the process optimization of t...

(This article belongs to the Special Issue Food Brewing Technology and Brewing Microorganisms (Second Edition))
  • Article
  • Open Access
33 Citations
10,892 Views
24 Pages

24 August 2020

There is a recognized need for formulating functional food products using selected lactic acid bacteria (LAB) starter cultures from various sources such as kefir, yoghurt or kombucha that have health benefits. The principle objective of this study wa...

(This article belongs to the Section Sensory and Consumer Sciences)
  • Article
  • Open Access
40 Citations
11,392 Views
18 Pages

Water Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In Vitro

  • Marta Calatayud,
  • Rosa Aragao Börner,
  • Jonas Ghyselinck,
  • Lynn Verstrepen and ,
  • Jelle De Medts,
  • Pieter Van den Abbeele,
  • Claire L. Boulangé,
  • Sarah Priour,
  • Massimo Marzorati and
  • Sami Damak
  • + 5 authors+ 0 author

29 October 2021

Fermentation is an ancient food preservation process, and fermented products have been traditionally consumed in different cultures worldwide over the years. The interplay between human gut microbiota, diet and host health is widely recognized. Diet...

(This article belongs to the Section Nutrition and Public Health)
  • Article
  • Open Access
5 Citations
4,286 Views
20 Pages

7 September 2022

Microorganism biomass is a sustainable and innovative source of biopolymers, such as proteins and polysaccharides, that is suitable for the development of biodegradable films. The aim of this research was to evaluate the synthesis, morphology, rheolo...

(This article belongs to the Special Issue Multifunctional Hybrid Materials Based on Polymers: Design and Performance, Volume II)
  • Article
  • Open Access
564 Views
17 Pages

Fortification of Water Kefir with Gallic Acid: Physicochemical Effects and Antioxidant Capacity in an In Vitro Digestion Model

  • Uriel Felipe Valdez-Olmos,
  • José Carlos Rodríguez Figueroa,
  • Ariadna Thalía Bernal-Mercado,
  • Fridha Viridiana Villalpando-Vargas and ,
  • Esther Sendra-Nadal and
  • Carmen Lizette Del-Toro-Sánchez
  • + 1 author+ 0 author

1 June 2026

Today, consumers are interested in healthier food. Fortifying water kefir (WK) with gallic acid (GA) could enhance kefir’s health benefits. The objective of this study was to evaluate the effects of GA-fortified WK (WK-GA) and pasteurization on...

(This article belongs to the Section Food Nutrition)
  • Article
  • Open Access
2,073 Views
16 Pages

Water kefir production requires the fermentation of sweetened water with polysaccharide starter culture embedded with bacteria and yeast, which determines the finished product’s sensory, microbial, and chemical profile. The culture self-propaga...

(This article belongs to the Section Beverage Technology Fermentation and Microbiology)
  • Article
  • Open Access
19 Citations
5,885 Views
14 Pages

Kombucha and Water Kefir Grains Microbiomes’ Symbiotic Contribution to Postbiotics Enhancement

  • Marina Pihurov,
  • Bogdan Păcularu-Burada,
  • Mihaela Cotârleț,
  • Leontina Grigore-Gurgu and ,
  • Daniela Borda,
  • Nicoleta Stănciuc,
  • Maciej Kluz and
  • Gabriela Elena Bahrim
  • + 3 authors+ 0 author

2 July 2023

Wild artisanal cultures, such as a symbiotic culture of bacteria and yeasts (SCOBY) and water kefir grains (WKG), represent a complex microorganism consortia that is composed of yeasts and lactic and acetic acid bacteria, with large strains of divers...

(This article belongs to the Special Issue Current Research on Probiotics and Fermented Products)
  • Article
  • Open Access
11 Citations
3,605 Views
13 Pages

Physical Characterization of a Novel Carrot Juice Whey-Enriched Beverage Fermented with Milk or Water Kefir Starter Cultures

  • Anita Rejdlová,
  • Richardos Nikolaos Salek,
  • Zuzana Míšková,
  • Eva Lorencová and ,
  • Vendula Kůrová,
  • Richard Adámek and
  • Daniela Sumczynski
  • + 2 authors+ 0 author

8 September 2023

The purpose of this work was to evaluate the selected physicochemical, rheological, and sensory properties of a new whey-enriched carrot juice beverage (carrot juice: whey ratios of 100:0; 95:5; 85:15; 75:25; 65:35) fermented with milk or water kefir...

(This article belongs to the Special Issue Recent Advances in Cheese and Fermented Milk Production)
  • Article
  • Open Access
43 Citations
5,865 Views
19 Pages

Improving the Functionality of Lentil–Casein Protein Complexes through Structural Interactions and Water Kefir-Assisted Fermentation

  • Mohammad Alrosan,
  • Thuan-Chew Tan,
  • Azhar Mat Easa,
  • Muhammad H. Alu’datt and ,
  • Carole C. Tranchant,
  • Ali Madi Almajwal,
  • Sana Gammoh,
  • Sofyan Maghaydah,
  • Mohammed Ali Dheyab and
  • Ali Al-Qaisi
  • + 6 authors+ 1 author

Highly nutritious lentil proteins (LP) have recently attracted interest in the food industry. However, due to their low solubility, extensive application of LP is severely limited. This study describes a new and successful method for overcoming this...

(This article belongs to the Special Issue Fermentation Processes: Modeling, Optimization and Control)
  • Article
  • Open Access
5 Citations
3,472 Views
17 Pages

12 December 2022

Nowadays, the use of biomass to produce cathode materials for lithium–sulfur (Li-S) batteries is an excellent alternative due to its numerous advantages. Generally, biomass-derived materials are abundant, and their production processes are envi...

(This article belongs to the Special Issue Advanced Electrode Materials for Batteries)
  • Article
  • Open Access
24 Citations
6,188 Views
22 Pages

Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells

  • Muganti Rajah Kumar,
  • Swee Keong Yeap,
  • Han Chung Lee,
  • Nurul Elyani Mohamad and ,
  • Muhammad Nazirul Mubin Aziz,
  • Melati Khalid,
  • Mas Jaffri Masarudin,
  • Adam Thean Chor Leow,
  • Janna Ong Abdullah and
  • Noorjahan Banu Alitheen
  • + 5 authors+ 0 author

Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six diffe...

(This article belongs to the Special Issue Antioxidants and Anti-inflammatory Effects in Neurodegenerative Diseases)
  • Review
  • Open Access
584 Views
24 Pages

The development of plant-based functional beverages is often limited by inherent matrix defects, particularly undesirable off-flavors, astringency, and colloidal instability. Water kefir (WK), a highly resilient multispecies symbiotic consortium, off...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
5 Citations
4,441 Views
26 Pages

Co-Cultivation of Potential Probiotic Strains Isolated from Water Kefir for Fermented Green Tea Beverage

  • Ameni Abdi,
  • Emna Gatri,
  • Justine Guilbaud,
  • Hassib Bouallagui and ,
  • Khaled Fadhlaoui,
  • Ghislain Garrait and
  • Lamia Ayed
  • + 2 authors+ 0 author

This study aimed to isolate and characterize microorganisms from water kefir beverage for their functional properties. Five lactic acid bacteria (LAB) strains were isolated: three Leuconostoc citreum strains (LB4, LB6, LB13) and two Lactococcus lacti...

  • Proceeding Paper
  • Open Access
6 Citations
6,236 Views
8 Pages

Evaluation of Polyphenol Content and Antioxidant Activity of Standard Water Kefir

  • Ecaterina-Andreea Constantin,
  • Ioana Popa-Tudor,
  • Florentina Matei,
  • Diana Constantinescu-Aruxandei and
  • Florin Oancea

This study focuses on the polyphenol levels and antioxidant activity in the water kefir beverage after 72 and 96 h of fermentation. The exopolysaccharides (dextran) were extracted from water kefir granules and characterized using Fourier-transform in...

(This article belongs to the Proceedings of Exploratory Workshop "Innovative Cross-Sectoral Technologies", Vth Edition, Secvent Project Meeting)
  • Article
  • Open Access
371 Views
37 Pages

4 October 2026

Orange juice was investigated as a substrate for the production of novel kefir-like beverages using milk kefir grains (MKG) and water kefir grains (WKG). Fermentation was monitored for 48 h to characterize changes in physicochemical parameters, subst...

(This article belongs to the Special Issue Emerging Trends in Food Microbiology and Food Safety)
  • Article
  • Open Access
2 Citations
2,251 Views
19 Pages

Comparative Study of Water and Milk Kefir Grains as Biopolymeric Adsorbents for Copper(II) and Arsenic(V) Removal from Aqueous Solutions

  • Balázs György,
  • Marek Bujdoš,
  • Hana Vojtková,
  • Pavel Diviš and ,
  • Michal Slaný,
  • Peter Matúš and
  • Martin Urík
  • + 2 authors+ 0 author

28 November 2024

This study investigates the biosorption capabilities of kefir grains, a polysaccharide-based byproduct of the fermentation process, for removing copper(II) and arsenic(V) from contaminated water. Unlike traditional heavy-metal removal methods, which...

(This article belongs to the Special Issue Advanced Polymers for Wastewater Treatment and Toxicant Removal)
  • Article
  • Open Access
4 Citations
1,563 Views
16 Pages

Shelf Life and Sensory Profile of Potentially Probiotic Mead Obtained by Co-Fermentation of Water Kefir and Saccharomyces boulardii

  • Handray Fernandes de Souza,
  • Ricardo Donizete Teixeira,
  • Fabiano Vaquero Silva Junior,
  • Felipe Donizete Teixeira and ,
  • Karina Nascimento Pereira,
  • Adriano Gomes da Cruz,
  • Igor Viana Brandi and
  • Eliana Setsuko Kamimura
  • + 3 authors+ 0 author

29 December 2025

Mead is an alcoholic beverage obtained through the action of yeast in a diluted solution of honey and water. According to the literature, recent studies have demonstrated the potential of the probiotic yeast Saccharomyces cerevisiae var. boulardii in...

(This article belongs to the Section Food Microbiology)
  • Article
  • Open Access
5 Citations
6,183 Views
25 Pages

Development, Characterization, and Stability of Flavored Water Kefir: Impact of Fermentation and Storage

  • Samarha Pacheco Wichello,
  • Kamila Ferreira Chaves,
  • Wallaf Costa Vimercati,
  • Sérgio Henriques Saraiva and
  • Luciano Jose Quintão Teixeira

The increasing demand for functional beverages sparked greater interest in health-promoting craft drinks, highlighting the need to optimize production parameters and assess their stability. This study aimed to develop, optimize, and characterize a gr...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Feature Paper
  • Review
  • Open Access
13 Citations
7,821 Views
31 Pages

Production of Kefir and Kefir-like Beverages: Fundamental Aspects, Advances, and Future Challenges

  • Marta Abajo Justel,
  • Eduardo Balvis Outeiriño and
  • Nelson Pérez Guerra

25 December 2025

Nowadays, consumer demand for functional foods with health benefits has grown significantly. In response to this trend, a variety of potentially probiotic foods have been developed—most notably kefir and kefir-like beverages, which are highly a...

(This article belongs to the Section Food Process Engineering)
  • Proceeding Paper
  • Open Access
1,117 Views
8 Pages

12 February 2026

Water kefir is a non-dairy fermented water drink which includes lactic acid bacteria, acetic acid bacteria and yeasts which provide probiotic as well as antioxidant properties. Prunus armeniaca (apricot) is a promising raw material to develop a funct...

(This article belongs to the Proceedings of The 4th International Electronic Conference on Processes)
  • Article
  • Open Access
22 Citations
7,205 Views
17 Pages

Non-Conventional Sucrose-Based Substrates: Development of Non-Dairy Kefir Beverages with Probiotic Potential

  • Pedro Paulo Lordelo Guimarães Tavares,
  • Clariane Teixeira Pessoa Mamona,
  • Renata Quartieri Nascimento,
  • Emanuele Araújo dos Anjos and ,
  • Carolina Oliveira de Souza,
  • Rogéria Comastri de Castro Almeida,
  • Maria Eugênia de Oliveira Mamede and
  • Karina Teixeira Magalhães-Guedes
  • + 3 authors+ 0 author

There is a scarcity of studies evaluating the influence of different commonly marketed sugars in water kefir beverage production. Therefore, this study aimed to evaluate the fermentation of water kefir grains in different sugary solutions: brown, dem...

(This article belongs to the Section Probiotic Strains and Fermentation)
  • Article
  • Open Access
15 Citations
4,766 Views
25 Pages

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

  • Monalisa de Alencar Lucena,
  • Igor Frederico da Silveira Ramos,
  • Maurycyo Silva Geronço,
  • Ricardo de Araújo and ,
  • Francisco Lopes da Silva Filho,
  • Luís Manuel Lopes Rodrigues da Silva,
  • Rayran Walter Ramos de Sousa,
  • Paulo Michel Pinheiro Ferreira,
  • Josy Anteveli Osajima and
  • Marcilia Pinheiro da Costa
  • + 8 authors+ 3 authors

17 June 2022

The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were st...

(This article belongs to the Special Issue Polymers in Biomedical Applications)
  • Article
  • Open Access
10 Citations
7,961 Views
31 Pages

Production and Optimisation of Fermented Pumpkin-Based Mature Coconut Water Kefir Beverage Using Response Surface Methodology

  • Wee Yin Koh,
  • Xiao Xian Lim,
  • Ban Hock Khor,
  • Babak Rasti and ,
  • Thuan Chew Tan,
  • Rovina Kobun and
  • Utra Uthumporn
  • + 2 authors+ 0 author

Fermentation of pumpkin puree and mature coconut water using water kefir grains is a potential method for producing a novel functional non-dairy-based probiotic drink. In the present study, response surface methodology based on Box–Behnken desi...

(This article belongs to the Topic Advances in Analysis of Food and Beverages)
  • Article
  • Open Access
8 Citations
2,434 Views
10 Pages

Blends of Chitosan and Water Kefir Grain Biomass Incorporated with Nanosilica

  • Carolina Oliveira Marinho,
  • Luís Marangoni Júnior,
  • Ricardo Rodrigo Ramos Cecci and
  • Roniérik Pioli Vieira

18 February 2023

The use of by-products from the food industry for the development of bioplastics represents an outstanding strategy in meeting current demands for material circularity. In this work, films based on chitosan (CH) and water kefir grain (WKG) biomass in...

(This article belongs to the Special Issue Research on Food Packaging and Storage)
  • Article
  • Open Access
1,565 Views
12 Pages

Water Kefir and Olive Leaf Polyphenols Attenuate Body Weight Gain and Excessive Adiposity in Diet-Induced Obese Rats

  • Miguel Lopez-Yoldi,
  • Amaia Huguet-Casquero,
  • Paula Aranaz,
  • José Ignacio Riezu-Boj and ,
  • Marian Fernández-Fernández,
  • Gorka Alkorta-Aranburu,
  • Dante Fratebianchi,
  • Eusebio Gainza and
  • Fermin I. Milagro
  • + 4 authors+ 0 author

Fermented foods and prebiotics are increasingly studied for their potential therapeutic roles in metabolic disorders. In this study, 52 male Wistar rats maintained on a high-fat, high-sucrose (HFS) diet were supplemented for 8 weeks with either water...

(This article belongs to the Topic Functional Foods and Nutraceuticals in Health and Disease)
  • Review
  • Open Access
43 Citations
9,920 Views
26 Pages

Novel Insights for Metabiotics Production by Using Artisanal Probiotic Cultures

  • Marina Pihurov,
  • Bogdan Păcularu-Burada,
  • Mihaela Cotârleţ,
  • Mihaela Aida Vasile and
  • Gabriela Elena Bahrim

Wild probiotic consortia of microorganisms (bacteria and yeasts) associated in the artisanal cultures’ microbiota (milk kefir grains, water kefir grains and kombucha) are considered valuable promoters for metabiotics (prebiotics, probiotics, postbiot...

(This article belongs to the Special Issue Microbial Diversity of Fermented Food)
  • Article
  • Open Access
641 Views
19 Pages

Microbial Diversity and Chemical Dynamics in Karanda Juice Kefir During Fermentation

  • Ramon Akkharapreechanont,
  • Pipat Macharoen,
  • Wanilada Rungrassamee and
  • Awanwee Petchkongkaew

Water kefir is a non-dairy fermented beverage widely recognised for its health benefits. The supplementation of fruits can further diversify product offerings while enhancing nutritional and functional value. In this study, karanda juice, derived fro...

(This article belongs to the Special Issue Fermented Foods in a Sustainable Future: Safety, Quality and Innovation)
  • Article
  • Open Access
4 Citations
1,857 Views
20 Pages

Optimising a Functional Beverage from Quinoa and Cherimoya Mixtures Fermented with Water Kefir Grains

  • Abigail E. Palacios-Castillo,
  • Tatiana N. Campoverde-Quilca,
  • Jimmy Núñez-Pérez,
  • Jhomaira L. Burbano-García and ,
  • Holger M. Pineda-Flores,
  • Rosario C. Espín-Valladares,
  • Santiago Zárate-Baca and
  • José-Manuel Pais-Chanfrau
  • + 3 authors+ 0 author

10 October 2025

Functional beverages enhance the nutritional value of their ingredients by increasing the levels of bioactive components, such as probiotics. To achieve consumer acceptance, functional beverages must be both palatable and nutritious. This study inves...

(This article belongs to the Topic Fermented Food: Health and Benefit)

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