Advancing Open Science

This paper develops a mechanism of PF-deletion within a probe–goal system that incorporates C-to-T feature inheritance. I propose that the phase head C enters the derivation not only with an edge feature (EF) and agree (φ-)features but also with a delete-feature, which licenses the deletion of an element at PF (PF-deletion). When C-to-T feature inheritance applies, the target of PF-deletion is determined through φ-probing from T; when it does not, it is determined through EF-probing from C. By linking PF-deletion to phase-internal probing, this approach dispenses with pro, traditionally assumed to exist in the lexicon of null subject languages such as Italian, as a theoretical primitive. Crucially, it offers a unified account of the distribution of null arguments in both Italian (a pro-drop language) and German (a topic-drop language), two language types that have traditionally resisted unified analysis under the principles-and-parameters approach. In addition to the synchronic study of the distribution of null arguments, I further argue that diachronic evidence from old languages such as Old French and Old English lends additional support to the proposal, and conclude that whether C-to-T inheritance applies or not is a crucial factor in explaining crosslinguistic variation in null argument phenomena.

Human neuroglobin (Ngb) is a globin featuring a disulfide bond (Cys46–Cys55) and a redox-active cysteine residue (Cys120) and plays a dual role in cellular stress responses. In this study, we investigated how wild-type (WT) Ngb and its two mutants, C120S Ngb, in which Cys120 is replaced by serine, and A15C Ngb, which contains an engineered Cys15–Cys120 disulfide bridge, modulate oxidative stress in triple-negative breast cancer (MDAMB231) and hormone receptor-positive breast cancer (MCF-7) cells. In both cell lines, WT Ngb enhanced cell survival under H₂O₂-induced oxidative stress by scavenging reactive oxygen species (ROS) through oxidation of Cys120. In contrast, the C120S and A15C mutants lost this protective capacity and instead promoted apoptosis. Mass spectrometry analysis confirmed the oxidation of Cys120 to sulfenic acid in WT Ngb, whereas both mutants exhibited impaired redox activity, leading to elevated ROS levels, lipid peroxidation, and activation of caspase-9/3. AO/EB staining further revealed that WT Ngb attenuated DNA damage, while the mutants exacerbated apoptosis in both MDAMB231 and MCF-7 cells. These results demonstrate that Cys120 acts as a critical redox switch, dictating whether Ngb exerts cytoprotective or pro-apoptotic effects across different breast cancer cell types. Our findings suggest that WT Ngb may help protect normal tissues during cancer therapy, whereas engineered Ngb mutants could be used to selectively sensitize both triple-negative and hormone receptor-positive breast cancer cells to oxidative damage, offering a novel redox-targeted therapeutic strategy.

Although particulate matter (PM) is strongly associated with allergic reactions, the potential risk of the ability of PM derived from poultry houses to induce allergic reactions remains unclear. This study investigated the effects of duck housing PM on allergic reactions in mice. PM samples and fungi were collected from a duck farm. Ovalbumin (OVA) was used as a positive control, with ambient-level concentrations of PM, high-concentration PM (HPM), and fungal experimental groups. Aerosol exposure was performed on the mice. Serum IgE, allergic mediators (histamines and leukotrienes), cytokines, and pulmonary histopathology were analyzed. Furthermore, HPM-induced metabolic profiles in bronchoalveolar lavage fluid were measured. The results revealed that all the treatment groups of mice presented allergic symptoms, including sneezing and coughing; higher concentrations of IgE, His, and LTs in the serum; upregulation of allergic reaction-related cytokines, such as IL4, IL5, and IL33; and microscopic lesions of the lungs characterized by inflammatory cell infiltration were observed in all the treatment groups, indicating that PM and fungi can cause allergic reactions. Notably, allergic reactions were more pronounced in the HPM and fungal groups than in the PM group. In addition, metabolomics analyses revealed that HPM exposure caused metabolic disorders in mouse lungs. The key pathway with the highest correlation to metabolite differences was pyrimidine metabolism, which is associated with allergic reactions. In conclusion, this study demonstrated that exposure to PM in duck houses can cause allergic reactions in mice and significant metabolomic changes in the lungs, especially HPM. Moreover, the contribution of fungal components in the PM cannot be ignored. These findings highlight the potential health risks associated with PM from the poultry industry.

Osteoporosis is a prevalent metabolic bone disorder characterized by reduced bone mass, compromised microarchitecture, and increased fracture risk. Its pathogenesis extends beyond simple bone mineral density (BMD) loss and reflects complex disruptions in bone remodeling governed by osteoblast–osteoclast coupling and systemic metabolic factors. This review lays particular emphasis on diabetes mellitus-related osteoporosis (DOP) and estrogen deficiency-induced osteoporosis (EDOP), discussing bone remodeling between osteoclastogenesis and osteoblast differentiation regulated by key signaling pathways, including the RANKL/RANK/OPG, Wnt/β-catenin, BMP–Smad, Hedgehog, and inflammatory cytokine networks. This review then explores how chronic hyperglycemia, insulin deficiency or resistance, oxidative stress, ferroptosis, advanced glycation end products, and low-grade inflammation disrupt bone homeostasis in diabetes, resulting in impaired bone quality and elevated fracture risk, particularly in type 2 diabetes. In parallel, we discuss the genomic and non-genomic actions of estrogen in maintaining skeletal integrity and elucidate how estrogen deficiency accelerates bone resorption and suppresses bone formation through altered cytokine signaling, oxidative stress, and impaired mechanotransduction. Advances in diagnostic strategies beyond BMD, including trabecular bone score, high-resolution peripheral quantitative computed tomography, and emerging biomarkers, are reviewed. Finally, this review summarizes current and emerging therapeutic approaches tailored to DOP and EDOP, emphasizing the need for mechanism-based, individualized management. A deeper understanding of these shared and distinct pathways may facilitate improved risk stratification and the development of targeted interventions for osteoporosis.

Bullying is not a dyadic interaction between victim and perpetrator, but a relational phenomenon involving multiple group networks: those who exercise physical, psychological, or symbolic violence; those who encourage it; those who suffer it; and those who, while aware of it, remain on the sidelines. Preventing bullying, or stopping it once it emerges, requires undermining the support base that sustains it: no one should play the role of cheerleader, and those who remain passive must become involved in defending those targeted. It is also necessary to foster in those who are bullied the strength and capacity to confront the situation. From a Freirean perspective, this implies weaving alliances between those who are kindred and those who are different, and even with outsiders, to oppose those who act antagonistically. Such a task demands debate, reflection, and the collective formulation of measures among the diverse group realities in schools, given that bullying is grounded in the refusal to recognize certain others as part of “us”, though we are all “others” to one another. This article sets out arguments for the need to address these diverse group realities and presents the phases and main contents of a participatory process for designing and implementing a School Coexistence Plan, drawing on the Participatory Construction of Peaceful Coexistence method as a framework for addressing bullying.

Flag leaf angle (FLA) in rice (Oryza sativa L.) is one of the important traits affecting F₁ seed production by mechanization. Here, we report the map-based cloning and functional characterization of the FLA1 (FLAG-LEAF-ANGLE 1) gene, which resides at a major-effect quantitative trait locus (QTL). Through cell morphological observations and exogenous hormone treatment assays, we demonstrate that gibberellin (GA) modulates rice FLA by altering both the number of cell layers and cell length. Combining genetic and molecular biological analyses with genetic complementation and gene overexpression assays, we elucidated and validated the biological function of FLA1. In addition, we found that FLA1 is constitutively expressed and encodes a protein localized to both the cell membrane and nucleus. Via RT-qPCR assays, we further demonstrated that the FLA1^fla-R allele from the rice accession fla-R enhances GA biosynthesis by upregulating the expression of CLA1 and GA20ox2. Furthermore, yeast two-hybrid assays revealed that auxin-repressed protein 1 (ARP1) interacts with FLA1, suggesting a potential role of this interaction in the modulation of rice FLA. Collectively, our results demonstrate that optimizing rice FLA via molecular manipulation of FLA1 can resolve the problem of flag leaf shearing during F₁ hybrid rice seed production without compromising F₁ hybrid seed yield, thereby facilitating mechanized F₁ hybrid rice seed production.

The fallopian tubes are critical segments of the female reproductive tract and are essential for transporting gametes and embryos. It creates a conducive environment necessary for successful fertilization, early embryo development, and embryo transport. The cellular composition and function of the fallopian tube are tightly regulated by the sex hormones estradiol and progesterone. Therefore, any pathological/ metabolic condition or exposure to exogenous agents with the potential to alter endocrine levels can have a significant impact on fallopian tube function and health. This review summarizes the effects of medications, infections, pathological conditions, lifestyle choices, and environmental factors that can significantly impact the morphology, histology, cellularity, and functionality of the fallopian tube.

To address the insufficient bearing capacity and severe deformation of narrow coal pillars in deep gob-side entries under the influence of residual dynamic loading and hydraulic punching of the coal mass, this study investigates the plastic-damage evolution mechanism of narrow pillars and proposes a novel “grip-anchoring (GA)” collaborative support system. A physical model testing system for narrow coal pillars reinforced by double-pull cable bolts was established based on similarity theory, and six support schemes were designed for comparative experiments. Digital image correlation was employed to analyze the displacement field and the evolution of plastic failure, and an industrial-scale field test was carried out to verify the reliability of the proposed support technology. The results indicate that the double-pull cable bolts, through a “dual-tensioning and synergistic locking” procedure, can effectively solve the support challenges of narrow coal pillars under asynchronous excavation. The dense double-row double-pull cable-bolt scheme maintained overall structural stability even under a 2.5p overload, with only localized damage occurring at the roof- and floor-corner zones of the pillar. This scheme exhibited the smallest deformation and the highest peak load among all tested configurations, demonstrating its significant advantage in enhancing structural stability.