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17 pages, 1058 KB

Open AccessArticle

Collagen Hydrolysate–Cranberry Mixture as a Functional Additive in Sausages

by Yasin Uzakov, Aziza Aitbayeva, Madina Kaldarbekova, Madina Kozhakhiyeva, Arsen Tortay and Kadyrzhan Makangali

Processes 2025, 13(10), 3233; https://doi.org/10.3390/pr13103233 (registering DOI) - 10 Oct 2025

Abstract

Consumers increasingly seek clean-label meat products with improved nutrition and stability. We evaluated a collagen hydrolysate–cranberry mixture (CH-CR) as a functional additive in cooked sausages. Two formulations—control and CH-CR—were assessed for fatty acid profile; lipid and protein oxidation during storage; antioxidant capacity ferric-reducing [...] Read more.

Consumers increasingly seek clean-label meat products with improved nutrition and stability. We evaluated a collagen hydrolysate–cranberry mixture (CH-CR) as a functional additive in cooked sausages. Two formulations—control and CH-CR—were assessed for fatty acid profile; lipid and protein oxidation during storage; antioxidant capacity ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and half-maximal inhibitory concentration (IC50); amino acid composition; and instrumental color. Relative to the control, CH-CR produced a more favorable lipid profile: lower saturated fatty acids (SFAs) 23.9% vs. 28.0%, higher monounsaturated fatty acids (MUFAs) 53.2% vs. 49.3%, slightly higher polyunsaturated fatty acids (PUFAs) 23.3% vs. 22.7%, a higher PUFA/SFA ratio of 0.97 vs. 0.81, and a lower omega-6/omega-3 (n-6/n-3) ratio of 13.5 vs. 27.1, driven by higher alpha-linolenic acid (ALA) 1.6% vs. 0.8%, with trans fats <0.1%. Storage studies showed attenuated oxidation in CH-CR: lower peroxide value (PV) at day 10 8.1 ± 0.4 vs. 9.8 ± 0.5 meq/kg and lower thiobarbituric acid-reactive substances (TBARS) at day 6 0.042 ± 0.004 vs. 0.055 ± 0.006 mg MDA/kg and day 10 0.156 ± 0.016 vs. 0.590 ± 0.041 mg MDA/kg); the acid value at day 10 was similar. Antioxidant capacity increased with CH-CR FRAP 30.5 mg gallic acid equivalents (GAE)/g vs. not detected; DPPH inhibition was 29.88% vs. 10.23%; IC50 56.22 vs. 149.51 µg/mL. The amino acid profile reflected collagen incorporation—higher glycine+proline+hydroxyproline 2.37 vs. 1.38 g/100 g and a modest rise in indispensable amino acids (IAAs) 5.72 vs. 5.42 g/100 g, increasing the IAA/total amino acid (TAA) ratio to 0.411 vs. 0.380. CH-CR samples were lighter and retained redness better under light, with comparable overall color stability. Overall, CH-CR is a natural strategy to improve fatty acid quality and oxidative/color stability in sausages. Full article

(This article belongs to the Special Issue Food Processing and Ingredient Analysis)

21 pages, 1041 KB

Open AccessArticle

Effects of Alternate Wetting and Drying (AWD) Irrigation on Rice Growth and Soil Available Nutrients on Black Soil in Northeast China

by Chaoyin Dou, Chen Qian, Yuping Lv and Yidi Sun

Agronomy 2025, 15(10), 2372; https://doi.org/10.3390/agronomy15102372 (registering DOI) - 10 Oct 2025

Abstract

Extensive practice has demonstrated that the continuous pursuit of high yields in the black soil region of Northeast China resulted in imbalances in soil nutrients and declines in both soil quality and water use efficiency. Alternate wetting and drying (AWD) irrigation offers a [...] Read more.

Extensive practice has demonstrated that the continuous pursuit of high yields in the black soil region of Northeast China resulted in imbalances in soil nutrients and declines in both soil quality and water use efficiency. Alternate wetting and drying (AWD) irrigation offers a promising solution for increasing rice yield and maintaining soil fertility. However, the success of this irrigation method largely depends on its scheduling. This study examined the threshold effects of AWD on rice growth, yield, and soil nutrient availability in the Sanjiang Plain, a representative black soil region in Northeast China. A two-year trial was conducted from 2023 to 2024 at the Qixing National Agricultural Science and Technology Park. “Longjing 31,” a local cultivar, was selected as the experimental material. The lower limit of soil water content under AWD was set as the experimental factor, with three levels: −10 kPa (LA), −20 kPa (MA), and −30 kPa (SA). The local traditional irrigation practice, continuous flooding, served as the control treatment (CK). Indicators of rice growth and soil nutrient content were measured and analyzed at five growth stages: tillering, jointing, heading, milk ripening, and yellow ripening. The results showed that, compared to CK, AWD had minimal impact on rice plant height and tiller number, with no significant differences (p > 0.05). However, AWD affected leaf area index (LAI), shoot dry matter (SDM), yield, and soil nutrient availability. In 2023, control had little effect on rice plant height and tiller number among the different irrigation treatments. The LAI of LA was 11.1% and 22.5% higher than that of MA and SA, respectively, while SDM in LA was 10.5% and 17.2% higher than in MA and SA. Significant differences were found between LA and MA, as well as between LA and SA, whereas no significant differences were observed between MA and SA. The light treatment is beneficial to the growth and development of rice, while the harsh growth environment caused by the moderate and severe treatments is unfavorable to rice growth. The average contents of nitrate nitrogen (NO₃⁻-N), available phosphorus (AP), and available potassium (AK) in LA were 11.4%, 8.4%, and 9.3% higher than in MA, and 16.7%, 11.5%, and 15.0% higher than in SA, respectively. Significant differences were observed between LA and SA. This is because the light treatment facilitates the release of available nutrients in the soil, while the moderate and severe treatments hinder this process. Although panicle number per unit area and grain number per panicle in LA were 7.5% and 2.3% higher than in MA, and 10.8% and 2.2% higher than in SA, these differences were not statistically significant. Seed setting rate and thousand-grain weight showed little variation across irrigation treatments. The yield of LA was 10,233.3 kg hm⁻², 9.1% and 14.1% higher than that of MA and SA, respectively, with significant differences observed. Compared with the moderate and severe treatments, the light treatment increases indicators such as the number of panicles per unit area, grains per panicle, thousand-grain weight, and seed setting rate, resulting in significant differences among the treatments. Water use efficiency (WUE) decreased as the control level increased. The WUE of all AWD irrigation treatments was significantly higher than that of the control treatment (CK). Compared with CK, AWD reduces evaporation, percolation, and other water losses, leading to a significant decrease in water consumption. Meanwhile, the yield remains basically unchanged or even slightly increases, thus resulting in a higher WUE than CK. The trends in rice growth, soil nutrient indicators, and WUE in 2024 were generally consistent with those observed in 2023. In 2024, the yield of LA was 9832.7 kg hm⁻², 14.9% and 17.3% higher than that of MA and SA, respectively, with significant differences observed. Based on the results, the following conclusions are drawn: (1) AWD irrigation can affect the growth of rice, alter the status of available nutrients in the soil, and thereby cause changes in yield and WUE; (2) LA is the optimal treatment for increasing rice yield, improving the availability of soil available nutrients, and improving WUE; (3) Both MA and SA enhanced WUE; however, these practices negatively impacted rice growth and the concentration of soil available nutrients, leading to a concurrent decline in yield. To increase rice yield and maintain soil fertility, LA, with an irrigation upper limit of 30 mm and a soil water potential threshold of −10 kPa, is recommended for the Sanjiang Plain region. Full article

(This article belongs to the Special Issue Influence of Irrigation and Water Use on Agronomic Traits of Crops—2nd Edition)

23 pages, 2035 KB

Open AccessArticle

Virtual Inertia of Electric Vehicle Fast Charging Stations with Dual Droop Control and Augmented Frequency Support

by Nargunadevi Thangavel Sampathkumar, Anbuselvi Shanmugam Velu, Brinda Rajasekaran and Kumudini Devi Raguru Pandu

Sustainability 2025, 17(20), 8997; https://doi.org/10.3390/su17208997 (registering DOI) - 10 Oct 2025

Abstract

High penetration of Inverter-Based Resources (IBRs) into the power grid could diminish the rotational inertia offered by a traditional power system and thus impact frequency stability. Several techniques are adopted to provide virtual inertial support to the grid for a short duration in [...] Read more.

High penetration of Inverter-Based Resources (IBRs) into the power grid could diminish the rotational inertia offered by a traditional power system and thus impact frequency stability. Several techniques are adopted to provide virtual inertial support to the grid for a short duration in the presence of IBRs. This paper uses the combined inertia support of a Dual Active Bridge (DAB) and a Voltage Source Converter (VSC)-fed Electric Vehicle Fast Charging System (EVFCS) is used to provide virtual inertia support to the grid. The Voltage Source Converter is designed to provide DC bus voltage regulation. Coordinated control of DAB converters and VSCs for mitigating frequency oscillations using cascaded droop-integrated Proportional Integral (PI) controllers is proposed. An aggregated low-frequency model of a DAB converter is considered in this work. The inertia of the DC link capacitor of the VSCs and battery is sequentially extracted to offer grid frequency support. In this work, the single droop control, dual droop control, grid-forming and Augmented Frequency Support (AFS) modes are explored to provide virtual inertia support to the grid. Full article

14 pages, 1672 KB

Open AccessArticle

Increasing Light Intensity Enhances Bacillus amyloliquefaciens PMB05-Mediated Plant Immunity and Improves Biocontrol of Bacterial Wilt

by Sin-Hua Li, Ai-Ting Li, Ming-Qiao Shi, Yi-Xuan Lu, Li-Ya Hong, Hsing-Ying Chung and Yi-Hsien Lin

Agriculture 2025, 15(20), 2110; https://doi.org/10.3390/agriculture15202110 (registering DOI) - 10 Oct 2025

Abstract

Bacterial wilt is a highly destructive disease affecting a wide range of crops, with no effective chemical control methods currently available. Consequently, the development of microbial strategies for disease management has become increasingly important. Among these, plant immunity-intensifying microbes have demonstrated promising efficacy [...] Read more.

Bacterial wilt is a highly destructive disease affecting a wide range of crops, with no effective chemical control methods currently available. Consequently, the development of microbial strategies for disease management has become increasingly important. Among these, plant immunity-intensifying microbes have demonstrated promising efficacy in controlling bacterial wilt. However, the influence of environmental factors, particularly light intensity, on the effectiveness of these microbes remains unclear. Light intensity is a critical regulator of the photosynthetic system and plant biochemical functions, including defense responses. In this study, we specifically utilized Arabidopsis plants grown under distinct light intensities to systematically examine how light conditions affect the induction of plant immune responses and the occurrence of bacterial wilt. Our findings revealed that Arabidopsis grown under high light intensity exhibited significantly stronger immune responses and reduced disease severity, compared to plants grown under low light intensity. Further, application of Bacillus amyloliquefaciens PMB05, a plant immunity-intensifying strain, resulted in more pronounced immune signaling and disease control efficacy under high light conditions. Experiments using salicylic acid (SA)-deficient mutants demonstrated that disruption of the SA pathway abolished the enhanced suppression of bacterial wilt conferred by B. amyloliquefaciens PMB05 under high light intensity, indicating that the SA pathway is indispensable for PMB05-mediated disease resistance. Moreover, the validation experiments in tomato plants supported these results, with B. amyloliquefaciens PMB05 significantly reducing bacterial wilt development under high light intensity. Collectively, our study demonstrates that growing plants under varying light intensities provides critical insights into how environmental conditions modulate the effectiveness of plant immunity-intensifying microbes, offering a potential strategy for integrated disease management in crops. Full article

(This article belongs to the Special Issue Biocontrol Agents for Plant Pest Management)

16 pages, 8519 KB

Open AccessArticle

The Oxidation and Corrosion Resistance of AlCrNbSiTiN Multi-Principal Element Nitride Coatings

by Zhenbo Lan, Jiangang Deng, Heng Xu, Zhuolin Xu, Zhengqi Wen, Wei Long, Lei Zhang, Ruoxi Wang, Jie Liu and Yanming Chen

Materials 2025, 18(20), 4663; https://doi.org/10.3390/ma18204663 (registering DOI) - 10 Oct 2025

Abstract

Multi-principal element nitrides have great application potential in protective coatings. However, the investigation of the oxidation and corrosion resistance of multi-principal element nitride coatings is still insufficient. The synthesis and high-temperature performance of AlCrNbSiTiN multi-principal element nitride coatings fabricated through optimized arc ion [...] Read more.

Multi-principal element nitrides have great application potential in protective coatings. However, the investigation of the oxidation and corrosion resistance of multi-principal element nitride coatings is still insufficient. The synthesis and high-temperature performance of AlCrNbSiTiN multi-principal element nitride coatings fabricated through optimized arc ion plating (AIP) were explored. Leveraging the high ionization efficiency and ion kinetic energy characteristic of AIP, coatings with significantly fewer internal defects were obtained. These coatings demonstrate superior mechanical properties, including a maximum hardness of 36.5 GPa and critical crack propagation resistance (CPR) values approaching 2000 N². Optimal coatings exhibited exceptional water vapor corrosion resistance (5.15 at% O after 200 h). The coatings prepared at −150 V had the optimal corrosion resistance, with the coating resistance and corrosion current density being 1.68 × 10⁴ Ω·cm² and 0.79 μA·cm⁻², respectively. AlCrNbSiTiN coatings produced under these optimized AIP conditions exhibit remarkably high-temperature oxidation, highlighting their potential for use in demanding engineering applications. Full article

(This article belongs to the Special Issue Advanced Science and Technology of High Entropy Materials)

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14 pages, 815 KB

Open AccessArticle

Changes in Gonadal Sex Differentiation, Digestive Enzymes, and Growth-Related Hormone Contents in the Larval and Juvenile Black Scraper, Thamnaconus modestus

by Wengang Xu, Yan Liu, Jiulong Wang, Pei Yang, Yanqing Wu and Liming Liu

Biology 2025, 14(10), 1385; https://doi.org/10.3390/biology14101385 (registering DOI) - 10 Oct 2025

Abstract

To understand the changes in gonadal sex differentiation, digestive enzyme activity, and growth-related hormone levels in the larval and juvenile black scraper, Thamnaconus modestus, continuous sampling was conducted from 0 to 91 days post-hatching (dph). 17β-estradiol (E2) and testosterone (T) levels, six [...] Read more.

To understand the changes in gonadal sex differentiation, digestive enzyme activity, and growth-related hormone levels in the larval and juvenile black scraper, Thamnaconus modestus, continuous sampling was conducted from 0 to 91 days post-hatching (dph). 17β-estradiol (E2) and testosterone (T) levels, six digestive enzymes, as well as T3, T4, GH, and IGF-I were detected. The results showed that oogonia or spermatogonia was observed at 60 dph. During the sex differentiation to female or male, both E2 and T levels significantly increased (p < 0.05), suggesting that E2 and T may induce the sex differentiation to female or male in T. modestus, respectively. The amylase activity from 0 to 35 dph showed a slow upward trend, which may be due to the transition from endogenous to exogenous nutrition at this time. From 12 to 25 dph, alkaline protease activity significantly decreased (p < 0.05), while acid protease levels significantly increased (p < 0.05), suggesting that as organs in the digestive system continue to develop, acid protease plays an important role. T3 and T4 could already be detected at 0 dph, and the T4 content was always much higher than T3 throughout the stages, indicating that T4 may play more important roles than T3. Additionally, the changes in IGF-I and GH content followed a trend of an initial increase, a subsequent decrease, and then an increase, ultimately showing an overall upward trend. These results indicate that T4, IGF-I, and GH play crucial roles in growth and development in the juvenile fish. In conclusion, the results of this study provide useful information for growth, artificial reproduction, and sex regulation in T. modestus. Full article

(This article belongs to the Section Evolutionary Biology)

20 pages, 1203 KB

Open AccessReview

Central Roles of Glucosylceramide in Driving Cancer Pathogenesis

by Xueheng Zhao and Manoj Kumar Pandey

Int. J. Mol. Sci. 2025, 26(20), 9879; https://doi.org/10.3390/ijms26209879 (registering DOI) - 10 Oct 2025

Abstract

Glucosylceramide (GlcCer), a central glycosphingolipid derived from ceramide, is increasingly recognized as a bioactive lipid that intersects with key metabolic, inflammatory, and oncogenic pathways. While its dysregulation has long been associated with lysosomal storage disorders such as Gaucher disease (GD), growing evidence implicates [...] Read more.

Glucosylceramide (GlcCer), a central glycosphingolipid derived from ceramide, is increasingly recognized as a bioactive lipid that intersects with key metabolic, inflammatory, and oncogenic pathways. While its dysregulation has long been associated with lysosomal storage disorders such as Gaucher disease (GD), growing evidence implicates GlcCer in cancer initiation and progression, particularly within tumor-predisposing conditions. GlcCer modulates membrane microdomains, intracellular trafficking, and cell signaling, counteracting ceramide-induced apoptosis and promoting cellular survival. In cancer, aberrant upregulation of UDP-glucose ceramide glucosyltransferase (UGCG), the enzyme responsible for GlcCer synthesis, drives tumor growth, metastasis, and multidrug resistance through activation of pathways such as phosphoinositide 3-kinase/protein kinase B (PI3K/Akt), mitogen-activated protein kinase (MAPK), canonical Wnt pathway (Wnt/β-catenin), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways. Specific GlcCer species (e.g., C16:0, C18:0, C24:1) display tissue-dependent functions, adding structural specificity to their oncogenic potential. Moreover, emerging links between GlcCer metabolism and chronic inflammation, oxidative stress, and altered glucose utilization highlight its role as a metabolic node bridging inherited metabolic disorders and malignancy. This review integrates recent advances in GlcCer biology, emphasizing its roles in tumor-predisposing diseases and exploring its potential as a biomarker and therapeutic target in oncology. Full article

(This article belongs to the Section Molecular Oncology)

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19 pages, 1339 KB

Open AccessArticle

Differential Modulation of Maize Silage Odor: Lactiplantibacillus plantarum vs. Lactiplantibacillus buchneri Drive Volatile Compound Change via Strain-Specific Fermentation

by Shuyuan Xue, Jianfeng Wang, Jing Yang, Yunjie Li, Jian He, Jiyu Han, Hongyan Xu, Xun Zhu and Nasi Ai

Agriculture 2025, 15(20), 2109; https://doi.org/10.3390/agriculture15202109 (registering DOI) - 10 Oct 2025

Abstract

Volatile organic compounds (VOCs) are critical indicators of the metabolic status of whole-plant maize silage (WPMS). However, the impact of inoculating various strains of fermentation agents on VOC changes has not been systematically explored. This study aimed to determine how inoculation with Lactiplantibacillus [...] Read more.

Volatile organic compounds (VOCs) are critical indicators of the metabolic status of whole-plant maize silage (WPMS). However, the impact of inoculating various strains of fermentation agents on VOC changes has not been systematically explored. This study aimed to determine how inoculation with Lactiplantibacillus plantarum and Lentilactobacillus buchneri modulates the VOC profile and odor of WPMS after 90 days. VOCs were extracted by headspace solid-phase microextraction and analyzed by gas chromatography-mass spectrometry (HS-SPME-GC-MS). Key VOCs were screened using the variable importance in projection (VIP) and substantiated by relative odor activity values (rOAV) and odor descriptions. A total of 82 compounds were identified, including 22 esters, 19 alcohols, 3 acids, 9 aldehydes, 2 ethers, 6 hydrocarbons, 4 ketones, 10 phenols, and 8 terpenoids. L. plantarum enhanced green/fruity odors while strain L. buchneri significantly reduced undesirable phenolic and aldehydic compounds. Six key VOCs influencing the odor of WPMS were selected: 4-ethyl-2-methoxyphenol and benzaldehyde, which contribute smoky, bacon, and bitter almond aromas, and (E)-3-hexen-1-ol, benzyl alcohol, (E, E)-2,4-heptadienal and methyl salicylate, which impart green, fruity, and nutty aromas. These findings highlight the effects and contributions of various strain additives on VOCs in WPMS, providing new theoretical insights for regulating the flavor profile of WPMS. Full article

(This article belongs to the Section Farm Animal Production)

17 pages, 7345 KB

Open AccessArticle

Cattle Abortions and Congenital Malformations Due to Bluetongue Virus Serotype 3 in Southern Belgium, 2024

by Laurent Delooz, Nick De Regge, Ilse De Leeuw, Frédéric Smeets, Thierry Petitjean, Fabien Grégoire and Claude Saegerman

Viruses 2025, 17(10), 1356; https://doi.org/10.3390/v17101356 (registering DOI) - 10 Oct 2025

Abstract

In July 2024, bluetongue virus serotype 3 (BTV-3) was first detected in southern Belgium, marking the onset of a major epidemic wave. This study documents, for the first time in Belgium, the ability of BTV-3 to cross the placental barrier in cattle, causing [...] Read more.

In July 2024, bluetongue virus serotype 3 (BTV-3) was first detected in southern Belgium, marking the onset of a major epidemic wave. This study documents, for the first time in Belgium, the ability of BTV-3 to cross the placental barrier in cattle, causing abortions and congenital central nervous system malformations. Abortion cases from January to December 2024 were monitored through the national abortion protocol, which mandates reporting and laboratory investigation (i.e., the year of emergence and the three previous years as the baseline data set). Among 5,751 reported abortions, 903 foetuses were tested by PCR, revealing widespread BTV-3 circulation. The first malformed PCR-positive foetus was recorded in mid-August, four weeks after a sharp increase in abortion rates. Lesions such as hydranencephaly were confirmed in PCR-positive foetuses, with a malformation rate of 32.24% in affected herds from weeks 36 to 52 (i.e., 22 times higher than in previous years). Gestational stage analysis indicated that congenital lesions were most frequent following infection between 70 and 130 days of gestation. Based on the observed gross lesions and the timing of abortion, it was deduced that the earliest maternal infections likely occurred in February–March 2024, implying low-level winter BTV-3 circulation before the official detection of the epidemic wave. These findings highlight the epidemiological value of systematic abortion monitoring as an early warning system tool and highlight the inadequacy of relying solely on clinical surveillance in adult ruminants. The abrupt emergence of BTV-3 across the territory without a gradual spatial spread underscores the need for anticipatory control strategies. Strategic, multivalent vaccination campaigns and enhanced abortion surveillance are critical to mitigate similar reproductive and economic losses in future bluetongue outbreaks. Full article

(This article belongs to the Special Issue Arboviral Diseases in Livestock)

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18 pages, 597 KB

Open AccessArticle

Upper Cervical Manipulation and Manual Massage Do Not Modulate Sympatho-Vagal Balance or Blood Pressure in Women: A Randomized, Placebo-Controlled Clinical Trial

by Estêvão Rios Monteiro, Linda S. Pescatello, Gustavo Henrique Garcia, Alexandre Gonçalves de Meirelles, Francine de Oliveira, Rafael Cotta de Souza, Leandro Alberto Calazans Nogueira, Agnaldo José Lopes and Daniel Moreira-Gonçalves

Healthcare 2025, 13(20), 2554; https://doi.org/10.3390/healthcare13202554 (registering DOI) - 10 Oct 2025

Abstract

Objectives: To compare the acute effects of upper cervical manipulation (CM) and manual massage (MM) to simulated CM (Sham) and Control conditions (Control) on heart rate variability (HRV) and blood pressure (BP) responses in women with non-elevated BP. Methods: A single-blind, [...] Read more.

Objectives: To compare the acute effects of upper cervical manipulation (CM) and manual massage (MM) to simulated CM (Sham) and Control conditions (Control) on heart rate variability (HRV) and blood pressure (BP) responses in women with non-elevated BP. Methods: A single-blind, four-arm, parallel-group, randomized, crossover, placebo-controlled trial recruited 15 apparently healthy women with non-elevated BP who visited the lab on four occasions with 48 h intervals to ensure adequate washout between interventions. A Latin square randomization approach was employed to assign participants to one of four experimental conditions: (1) Control: Rest without intervention; (2) CM: Bilateral high-velocity, low-amplitude manipulation of the upper cervical spine (C0–C2); (3) MM: A single 120 s session of MM release applied unilaterally to the anterior and posterior thigh, posterior lower leg, and lumbar musculature; or (4) Sham: Mimicking the positioning used in CM without the application of thrust manipulation. In each experiment, HRV, systolic and diastolic BP were measured at rest (Baseline) and every 15 min for 60 min after each intervention. All procedures were performed in the morning to avoid any confounding circadian rhythm effect on HRV and BP. Results: We found significant increases within conditions for RMSSDms (Control: Post-0 (p = 0.032), Post-15 (p = 0.023); Sham: Post-15 (p = 0.014); CM: Post-15 (p = 0.027)); SDNNms (Control: Post-45 (p = 0.037); CM: Post-45 (p = 0.014) and Post-60 (p = 0.019)); PNN50% (CM: Post-0 (p = 0.044), Post-15 (p = 0.044) and Post-45 (p = 0.019)); LF Power (ms2) (CM: Post-60 (p = 0.001)), and LF/HF ratio (MM: Post-60 (p = 0.022). Conclusions: Although no statistically significant between-condition differences were detected, within-condition changes with moderate-to-large effect sizes suggest potential clinical relevance of CM and MM. These preliminary findings emphasize the importance of effect sizes and may indicate greater translational significance in populations with non-elevated cardiovascular risk. Full article

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21 pages, 6028 KB

Open AccessArticle

Computer-Aided Molecular Design Meets Network Toxicology and Molecular Docking: A Joint Strategy to Explore Common Molecular Mechanisms of Phthalates on Human Breast Cancer and Structure–Activity Relationship

by Xinyu Yang, Zijun Bai, Xiaoyun Yan, Yu Zhou, Caiyun Zhong and Jieshu Wu

Int. J. Mol. Sci. 2025, 26(20), 9878; https://doi.org/10.3390/ijms26209878 (registering DOI) - 10 Oct 2025

Abstract

Distinct PAEs are implicated in breast cancer progression through multiple molecular pathways. This study aims to elucidate the potential mechanisms in common by which PAEs promote breast cancer progression. Dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), and diethylhexyl phthalate (DEHP) were selected as [...] Read more.

Distinct PAEs are implicated in breast cancer progression through multiple molecular pathways. This study aims to elucidate the potential mechanisms in common by which PAEs promote breast cancer progression. Dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), and diethylhexyl phthalate (DEHP) were selected as representative PAE compounds. Network toxicology guided the construction of a regulatory network centered on five key transcription factor-associated genes: TP53, CTNNB1, PPARA, ESR1, and CDKN2A. Differential expression and survival analyses confirmed the significant impact of these hub genes on breast cancer (p < 0.05). Molecular docking results revealed direct interactions between the three PAEs and hub targets, while BBP had the strongest PAE-hub gene interaction and DEHP had the weakest one. Computer-aided molecular design (CAMD), combined with molecular docking, found the importance of alkyl chains and phenyl in PAE-hub gene interaction. A group addition/subtraction controlled experiment revealed that the binding affinities of modified BBP variants to hub genes are all weaker than the unmodified parent. The drop was significant whether the C17 alkyl chain was lengthened to match DEHP (p = 0.026) or the phenyl group was removed (p = 0.022). The findings provide novel insights into the mechanism in common of PAE-promoting breast cancer and offer a foundation for the unified intervention strategies and the design of safer plasticizer alternatives. Full article

(This article belongs to the Section Molecular Toxicology)

22 pages, 1670 KB

Open AccessArticle

Evolution of Size, Mass, and Density of Galaxies Since Cosmic Dawn

by Rajendra P. Gupta

Galaxies 2025, 13(5), 115; https://doi.org/10.3390/galaxies13050115 - 10 Oct 2025

Abstract

The formation and evolution of galaxies and other astrophysical objects have become of great interest, especially since the launch of the James Webb Space Telescope in 2021. The mass, size, and density of objects in the early universe appear to be drastically different [...] Read more.

The formation and evolution of galaxies and other astrophysical objects have become of great interest, especially since the launch of the James Webb Space Telescope in 2021. The mass, size, and density of objects in the early universe appear to be drastically different from those predicted by the standard cosmology—the ΛCDM model. This work shows that the mass–size–density evolution is not surprising when we use the CCC+TL cosmology, which is based on the concepts of covarying coupling constants in an expanding universe and the tired light effect contributing to the observed redshift. This model is consistent with supernovae Pantheon+ data, the angular size of the cosmic dawn galaxies, BAO, CMB sound horizon, galaxy formation time scales, time dilation, galaxy rotation curves, etc., and does not have the coincidence problem. The effective radii r_e of the objects are larger in the new model by r_e∝ (1 + z)^0.93. Thus, the object size evolution in different studies, estimated as r_e ∝ (1 + z)^s with s = −1.0±0.3, is modified to r_e∝ (1 + z)^s+0.93, the dynamical mass by (1 + z)^0.93, and number density by (1 + z)^−2.80. The luminosity modification increases slowly with z to 1.8 at z = 20. Thus, the stellar mass increase is modest, and the luminosity and stellar density decrease are mainly due to the larger object size in the new model. Since the aging of the universe is stretched in the new model, its temporal evolution is much slower (e.g., at z = 10, the age is about a dex longer); stars, black holes, and galaxies do not have to form at unrealistic rates. Full article

20 pages, 1071 KB

Open AccessArticle

Power-Based Statistical Detection of Substance Accumulation in Constrained Places Using a Contact-Less Passive Magnetoelastic Sensor

by Ioannis Kalyvas and Dimitrios Dimogianopoulos

Vibration 2025, 8(4), 64; https://doi.org/10.3390/vibration8040064 (registering DOI) - 10 Oct 2025

Abstract

A contactless passive magnetoelastic sensing setup, recently proposed for detecting pest/substance accumulation in confined spaces (labs, museum reserves), is optimized for enhanced low-frequency performance. The setup uses a short flexible polymer slab, clamped at one end. There, a short Metglas^® 2826MB magnetoelastic [...] Read more.

A contactless passive magnetoelastic sensing setup, recently proposed for detecting pest/substance accumulation in confined spaces (labs, museum reserves), is optimized for enhanced low-frequency performance. The setup uses a short flexible polymer slab, clamped at one end. There, a short Metglas^® 2826MB magnetoelastic ribbon is fixed upon the slab’s surface. The opposite end receives excitation by a remotely controlled module of ultra-low amplitude vibration. When vibrating (with the slab), the ribbon generates magnetic flux, which depends on (and reflects) the slab’s dynamics. This changes when loads accumulate on its surface. The flux induces voltage in a contactless manner in a low-cost pick-up coil suspended above the ribbon. Voltage monitoring allows for evaluation of the vibrating slab’s real-time dynamics and, consequently, the detection of load-induced changes. This work innovates by introducing a low-cost passive circuit for real-time voltage processing, thus achieving an accurate representation of the low-frequency dynamics of the magnetic flux. Furthermore, it introduces an algorithm, which statistically detects load-induced changes using the voltage’s low-frequency power characteristics. Both additions enable load detection at relatively low frequencies, thus addressing a principal issue of passive contactless sensing setups. Extensive testing at different occasions demonstrates promising load detection performance under various conditions, especially given its cost-efficient hardware and operation. Full article

19 pages, 1457 KB

Open AccessArticle

Development and Evaluation of Hyaluronic Acid-Chitosan Coated Liposomes for Enhanced Delivery of Resveratrol to Breast Cancer Cells

by Yin Yin Myat, Khin Khin Gyi, Pornthida Riangjanapatee, Chuda Chittasupho, Songyot Anuchapreeda and Siriporn Okonogi

Polysaccharides 2025, 6(4), 93; https://doi.org/10.3390/polysaccharides6040093 (registering DOI) - 10 Oct 2025

Abstract

Resveratrol (RES), a naturally occurring polyphenolic compound with well-documented anticancer potential, is limited in clinical application due to its poor aqueous solubility and low bioavailability. This study aimed to develop RES-loaded liposomes coated sequentially with chitosan (CS) and hyaluronic acid-chitosan (HA) (RES-HA-CS-Lip) to [...] Read more.

Resveratrol (RES), a naturally occurring polyphenolic compound with well-documented anticancer potential, is limited in clinical application due to its poor aqueous solubility and low bioavailability. This study aimed to develop RES-loaded liposomes coated sequentially with chitosan (CS) and hyaluronic acid-chitosan (HA) (RES-HA-CS-Lip) to enhance RES stability, delivery, and anticancer efficacy in breast cancer cells. HA-CS-coated liposomes were prepared using a thin-film hydration technique. Their physicochemical characteristics were thoroughly investigated through dynamic light scattering, transmission electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The optimized RES-HA-CS-Lip exhibited spherical morphology with an average particle size of 212 nm, a narrow polydispersity index (<0.4), a zeta potential of +9.04 ± 1.0 mV, and high entrapment efficiency of 82.16%. Stability studies demonstrated superior retention of size, surface charge, and encapsulation efficiency over 28 days at both 4 °C and 25 °C. In vitro release profiles at physiological and acidic pH revealed sustained drug release, with enhanced release under acidic conditions mimicking the tumor microenvironment. Antioxidant activity, assessed via DPPH and ABTS radical-scavenging assays, indicated that RES retained its radical-scavenging potential upon encapsulation. Cytotoxicity assays demonstrated markedly improved anticancer activity against MCF-7 breast cancer cells, with an IC₅₀ of 13.08 μg/mL at 48 h, while maintaining high biocompatibility toward normal HaCaT keratinocytes. RES-HA-CS-Lip demonstrated excellent stability against degradation and aggregation. Overall, these findings highlight HA-CS-coated liposomes as a promising polysaccharide-based nanocarrier that enhances stability, bioactivity, and therapeutic efficacy of RES, representing a potential strategy for targeted breast cancer therapy. Full article

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26 pages, 2716 KB

Open AccessArticle

Infrared Target Detection Based on Image Enhancement and an Improved Feature Extraction Network

by Peng Wu, Zhen Zuo, Shaojing Su and Boyuan Zhao

Drones 2025, 9(10), 695; https://doi.org/10.3390/drones9100695 (registering DOI) - 10 Oct 2025

Abstract

Small unmanned aerial vehicles (UAVs) pose significant security challenges due to their low detectability in infrared imagery, particularly when appearing as small, low-contrast targets against complex backgrounds. This paper presents a novel infrared target detection framework that addresses these challenges through two key [...] Read more.

Small unmanned aerial vehicles (UAVs) pose significant security challenges due to their low detectability in infrared imagery, particularly when appearing as small, low-contrast targets against complex backgrounds. This paper presents a novel infrared target detection framework that addresses these challenges through two key innovations: an improved Gaussian filtering-based image enhancement module and a hierarchical feature extraction network. The proposed image enhancement module incorporates a vertical weight function to handle abnormal feature values while preserving edge information, effectively improving image contrast and reducing noise. The detection network introduces the SODMamba backbone with Deep Feature Perception Modules (DFPMs) that leverage high-frequency components to enhance small target features. Extensive experiments on the custom SIDD dataset demonstrate that our method achieves superior detection performance across diverse backgrounds (urban, mountain, sea, and sky), with mAP@0.5 reaching 96.0%, 74.1%, 92.0%, and 98.7%, respectively. Notably, our model maintains a lightweight profile with only 6.2M parameters and enables real-time inference, which is crucial for practical deployment. Real-world validation experiments confirm the effectiveness and efficiency of the proposed approach for practical UAV detection applications. Full article

21 pages, 1074 KB

Open AccessReview

Bidirectional Regulation of Nitric Oxide and Endothelin-1 in Cerebral Vasospasm: Mechanisms and Therapeutic Perspectives

by Katrin Becker and Kaihui Lu

Future Pharmacol. 2025, 5(4), 59; https://doi.org/10.3390/futurepharmacol5040059 (registering DOI) - 10 Oct 2025

Abstract

Cerebral vasospasm (CVS) following a subarachnoid hemorrhage (SAH) is a critical complication driven by imbalances between vasodilators and vasoconstrictors. This review explores the bidirectional interplay between nitric oxide (NO) and endothelin-1 (ET-1) in CVS pathogenesis. NO, a potent vasodilator mainly produced by endothelial [...] Read more.

Cerebral vasospasm (CVS) following a subarachnoid hemorrhage (SAH) is a critical complication driven by imbalances between vasodilators and vasoconstrictors. This review explores the bidirectional interplay between nitric oxide (NO) and endothelin-1 (ET-1) in CVS pathogenesis. NO, a potent vasodilator mainly produced by endothelial and neuronal nitric oxide synthase (eNOS/nNOS) under normal physiological conditions, is scavenged early after SAH by hemoglobin derivatives, leading to microcirculatory dysfunction, pericyte constriction, and impaired neurovascular coupling. Conversely, ET-1 exacerbates vasoconstriction by suppressing NO synthesis via ROS-dependent eNOS uncoupling and Rho-kinase activation. The NO/ET-1 axis further influences delayed cerebral ischemia (DCI) through mechanisms like 20-HETE-mediated cGMP suppression and oxidative stress. Emerging therapies—including NO donors, NOS gene therapy, and ET-1 receptor antagonists—aim to restore this balance. Understanding these pathways offers translational potential for mitigating CVS and improving outcomes post-SAH. Full article

16 pages, 1069 KB

Open AccessSystematic Review

Negative Pressure Wound Therapy for Surgical Site Infection Prevention Following Pancreaticoduodenectomy: A Systematic Review and Meta-Analysis

by Musaed Rayzah, Nasser A. N. Alzerwi, Bandar Idrees, Ahmed A. Alhumaid, Yaser Baksh, Afnan Alsultan and Fares Rayzah

Surgeries 2025, 6(4), 88; https://doi.org/10.3390/surgeries6040088 (registering DOI) - 10 Oct 2025

Abstract

Background/Objectives: Surgical site infections (SSIs) following pancreaticoduodenectomy contribute to significant morbidity and healthcare costs. Negative pressure wound therapy (NPWT) has emerged as a potential preventive intervention; however, evidence regarding its efficacy in pancreatic surgery remains limited. This systematic review and meta-analysis aimed to [...] Read more.

Background/Objectives: Surgical site infections (SSIs) following pancreaticoduodenectomy contribute to significant morbidity and healthcare costs. Negative pressure wound therapy (NPWT) has emerged as a potential preventive intervention; however, evidence regarding its efficacy in pancreatic surgery remains limited. This systematic review and meta-analysis aimed to evaluate the efficacy of NPWT compared to conventional dressings in preventing SSI following pancreaticoduodenectomy. Methods: PubMed, Scopus, BASE, Cochrane CENTRAL, and ClinicalTrials.gov were systematically searched from their inception to 2 April 2025. Randomized clinical trials and observational studies comparing NPWT with conventional dressings in patients undergoing pancreaticoduodenectomy were included. Two independent reviewers extracted the data and assessed the methodological quality. Random-effects meta-analysis was performed to calculate the pooled relative risks (RRs) with 95% CIs. The primary outcome was the incidence of SSI. The secondary outcomes included pancreatic fistula, seroma formation, incisional hernia, and readmission rates. Results: Nine studies (three randomized clinical trials and six observational studies) comprising 1247 patients were included. NPWT was associated with a significant reduction in SSI compared with conventional dressings (RR, 0.61; 95% CI, 0.41–0.90). Subgroup analysis revealed varying effects by study design: retrospective cohort studies showed a nonsignificant trend toward SSI reduction (RR, 0.53; 95% CI, 0.19–1.48), randomized clinical trials demonstrated a nonsignificant trend favoring NPWT (RR, 0.67; 95% CI, 0.37–1.23), and the single prospective cohort study showed significant SSI reduction (RR, 0.48; 95% CI, 0.28–0.84). No significant differences were observed in pancreatic fistula rates between the NPWT and conventional dressing groups. Prophylactic NPWT application, longer duration (≥5 days), and higher negative pressure settings (−125 mmHg) appeared more effective than therapeutic application, shorter duration, and lower-pressure settings, respectively. Conclusions: This systematic review and meta-analysis suggests that NPWT is associated with a reduced SSI risk following pancreaticoduodenectomy. The greatest benefit may be achieved with prophylactic application in high-risk patients, longer therapy duration, and higher negative pressure settings. These findings support the consideration of NPWT as part of SSI prevention strategies in pancreatic surgery, particularly for patients with identified risk factors. Full article

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12 pages, 1061 KB

Open AccessArticle

On the Possible Nature of White Holes

by Mikhail Pekker and Mikhail N. Shneider

Astronomy 2025, 4(4), 18; https://doi.org/10.3390/astronomy4040018 (registering DOI) - 10 Oct 2025

Abstract

This paper considers non-singular black holes. It discusses the observation of particles falling onto ordinary and non-singular black holes from the perspective of a distant observer. It is demonstrated that, during a stage in the evolution of non-singular black holes, powerful energy fluxes [...] Read more.

This paper considers non-singular black holes. It discusses the observation of particles falling onto ordinary and non-singular black holes from the perspective of a distant observer. It is demonstrated that, during a stage in the evolution of non-singular black holes, powerful energy fluxes can be emitted. Distant observers may interpret these fluxes as white holes. Full article

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22 pages, 2880 KB

Open AccessArticle

Tailoring Rheological, Viscoelastic, and Starch Structural Properties in Plant-Based Beverages via Homolactic Fermentation of Quinoa and Chickpea Flour Blends

by John Hurtado-Murillo, Wendy Franco and Ingrid Contardo

Polysaccharides 2025, 6(4), 92; https://doi.org/10.3390/polysaccharides6040092 (registering DOI) - 10 Oct 2025

Abstract

This study investigated the effects of homolactic fermentation on the rheological, viscoelastic, and starch structural properties of quinoa–chickpea flour-based beverages. Three formulations with increasing proportions of chickpea flour (10, 25, and 50%) were fermented for 10 h with Lactobacillus acidophilus LA-5. Apparent viscosity, [...] Read more.

This study investigated the effects of homolactic fermentation on the rheological, viscoelastic, and starch structural properties of quinoa–chickpea flour-based beverages. Three formulations with increasing proportions of chickpea flour (10, 25, and 50%) were fermented for 10 h with Lactobacillus acidophilus LA-5. Apparent viscosity, deformation capacity, storage modulus (G′), and pasting behavior were measured along with FTIR-based analysis of the starch molecular structure. All fermented samples reached pH values < 4.5 and exhibited improved rheological properties with significant increases in viscosity and storage modulus (G′), particularly in the 50:50 blend. These enhancements were attributed to the synergistic effects of homolactic fermentation and inherent properties of chickpea starch, particularly its high amylose content, large granule size, and long amylopectin chains. FTIR analysis revealed that the short-range molecular order of starches was preserved after fermentation in all beverages, except for the 50:50 blend, as evidenced by the increased degree of order (DO) and double helix (DD) ratios. Overall, these findings demonstrate that integrating chickpea flour and controlled homolactic fermentation is an effective strategy for tailoring the viscosity and stability of plant-based probiotic beverages, providing a theoretical basis for the development of clean-label and functional fermented plant-based systems. Full article

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12 pages, 502 KB

Open AccessArticle

Substitution of Fossil-Based Solvents in Organic Coatings

by Elias Rippatha, Hector Rolando Mendez Rossal, Bernhard Strauß and Clemens Schwarzinger

Clean Technol. 2025, 7(4), 88; https://doi.org/10.3390/cleantechnol7040088 (registering DOI) - 10 Oct 2025

Abstract

In this work a multi-criteria analysis and an optimization tool were developed, which allows the substitution of fossil-based solvents with bio-based alternatives based on Hansen solubility parameters and various physical parameters, such as the boiling point, evaporation rate, viscosity or wetting behavior. The [...] Read more.

In this work a multi-criteria analysis and an optimization tool were developed, which allows the substitution of fossil-based solvents with bio-based alternatives based on Hansen solubility parameters and various physical parameters, such as the boiling point, evaporation rate, viscosity or wetting behavior. The proof of concept was achieved by formulating two different paints used in coil coatings using the bio-based solvents, and they performed equally as well as their fossil-based counterparts. A potential decrease in CO₂ emissions was determined by a life cycle assessment and cradle-to-grave analysis of bio- and fossil-based solvents, which showed a large sustainability bonus when using solvents based on biomass. The introduced methodology provides initial insights into substituting currently used solvents systematically. Overall, implementing bio-based solvents is a viable drop-in method to decrease the environmental impact of paints and coatings, while maintaining the same performance. Full article

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17 pages, 1903 KB

Open AccessArticle

Evaluation of Reuse of Spent Mushroom Substrate for New Pleurotus ostreatus Crop Cycle

by Wagner Gonçalves Vieira Junior, Lucas da Silva Alves, Jadson Belém de Moura, Adriano Taffarel Camargo de Paula, Marcos Antônio da Silva Freitas, Manuel Álvarez Orti, Francisco José Gea Alegría and Diego Cunha Zied

AgriEngineering 2025, 7(10), 342; https://doi.org/10.3390/agriengineering7100342 (registering DOI) - 10 Oct 2025

Abstract

Although considered relatively sustainable, mushroom production generates significant waste at the end of cultivation. This study investigated the reuse of Spent Mushroom Substrate (SMS) to formulate new substrates for Pleurotus ostreatus cultivation. Substrates with high (higher bran content) and low (lower bran content) [...] Read more.

Although considered relatively sustainable, mushroom production generates significant waste at the end of cultivation. This study investigated the reuse of Spent Mushroom Substrate (SMS) to formulate new substrates for Pleurotus ostreatus cultivation. Substrates with high (higher bran content) and low (lower bran content) nitrogen levels were prepared and supplemented with 5%, 10%, or 20% SMS across three successive cycles P. ostreatus crops. Cultivation performance was evaluated based on biological efficiency, number of mushrooms, fresh weight, and number of clusters. Substrates were chemically characterized for total nitrogen, carbon, C/N ratio, electrical conductivity, and pH. The inclusion of SMS, along with reduced bran content, did not improve P. ostreatus yield and led to lower productivity compared to control substrates. No consistent correlations were observed between chemical variables and yield, although high-N substrates generally performed better. SMS reuse, under these conditions, is not viable, but results encourage further research. Full article

(This article belongs to the Section Sustainable Bioresource and Bioprocess Engineering)

17 pages, 2603 KB

Open AccessArticle

The Effect of Visual Attention Dispersion on Cognitive Response Time

by Yejin Lee and Kwangtae Jung

J. Eye Mov. Res. 2025, 18(5), 52; https://doi.org/10.3390/jemr18050052 (registering DOI) - 10 Oct 2025

Abstract

In safety-critical systems like nuclear power plants, the rapid and accurate perception of visual interface information is vital. This study investigates the relationship between visual attention dispersion measured via heatmap entropy (as a specific measure of gaze entropy) and response time during information [...] Read more.

In safety-critical systems like nuclear power plants, the rapid and accurate perception of visual interface information is vital. This study investigates the relationship between visual attention dispersion measured via heatmap entropy (as a specific measure of gaze entropy) and response time during information search tasks. Sixteen participants viewed a prototype of an accident response support system and answered questions at three difficulty levels while their eye movements were tracked using Tobii Pro Glasses 2. Results showed a significant positive correlation (r = 0.595, p < 0.01) between heatmap entropy and response time, indicating that more dispersed attention leads to longer task completion times. This pattern held consistently across all difficulty levels. These findings suggest that heatmap entropy is a useful metric for evaluating user attention strategies and can inform interface usability assessments in high-stakes environments. Full article

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19 pages, 6468 KB

Open AccessArticle

Assessment of the Permanent Gully Morphology Measurement by Unmanned Aerial Vehicle Photogrammetry with Different Flight Schemes in Dry–Hot Valley of Southwest China

by Ji Yang, Yifan Dong, Jiangcheng Huang, Xiaoli Wen, Guanghai Wang and Xin Zhao

Drones 2025, 9(10), 696; https://doi.org/10.3390/drones9100696 (registering DOI) - 10 Oct 2025

Abstract

Unmanned Aerial Vehicle (UAV) photogrammetry technique offers significant potential for generating highly detailed digital surface models (DSM) of gullies. However, different flight schemes can considerably influence measurement accuracy. The objectives were (i) to evaluate the influences of flight altitude, photo overlap, Ground Control [...] Read more.

Unmanned Aerial Vehicle (UAV) photogrammetry technique offers significant potential for generating highly detailed digital surface models (DSM) of gullies. However, different flight schemes can considerably influence measurement accuracy. The objectives were (i) to evaluate the influences of flight altitude, photo overlap, Ground Control Points (GCPs), and other environmental factors on the accuracy of the UAV-derived DSMs and (ii) to analyze the main factors affecting the accuracy of UAV gully monitoring and explore flight schemes that balance accuracy and efficiency. The results indicated that DSM accuracy improved markedly as the number of GCPs increased from 0 to 3, with consideration given to both horizontal and vertical distribution. However, further increases in the number of GCPs did not lead to significant improvements. The accuracy of DSMs increased with a decrease in the flight altitude, but was not substantially affected by photo overlap when it exceeded 50%/40% The accuracy of DSM was significantly reduced by shadows, and flight altitude rather than slope gradient was identified as the key factor leading to high-error checkpoints (error > 0.1 m). The proportion of point clouds penetrating tree canopies decreased when the flight altitude was 150 m or higher, which could help reduce the influence of vegetation on the accuracy of DSMs. In general, with a reasonable spatial distribution of GCPs, flight altitude is the primary factor affecting monitoring accuracy. However, when balancing accuracy and efficiency, the optimal flight scheme was determined to be a flight altitude of 70 m, photo overlap of 80%/70%, and nine GCPs. Full article

(This article belongs to the Section Drones in Ecology)

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27 pages, 1767 KB

Open AccessArticle

AppER: Design and Validation of a Mobile Application for Caregivers of Patients with Duchenne Muscular Dystrophy and Their Families in Spain and Latin America

by Jaume Barrera, Imanol Amayra, David Contreras, Alicia Aurora Rodríguez, Nicole Passi, Javiera Ortega and Óscar Martínez

Muscles 2025, 4(4), 43; https://doi.org/10.3390/muscles4040043 (registering DOI) - 10 Oct 2025

Abstract

Aim: The study developed and validated AppER, an mHealth tool for informal caregivers of children with Duchenne Muscular Dystrophy, and examined differences between app users and non-users. Methods: Four phases were followed: (1) focus groups with experts and caregivers to identify care-related domains; [...] Read more.

Aim: The study developed and validated AppER, an mHealth tool for informal caregivers of children with Duchenne Muscular Dystrophy, and examined differences between app users and non-users. Methods: Four phases were followed: (1) focus groups with experts and caregivers to identify care-related domains; (2) prototype development and validity testing (CVR, I-CVI, I-FVI) using the MARS scale; (3) implementation of the final app; and (4) psychosocial profiling of 88 caregivers (42 users and 46 non-users) measuring quality of life, dependency, somatic symptoms, and coping strategies. Results: AppER showed high content and face validity, surpassing reference thresholds. In the psychosocial analysis, users reported lower perceived quality of life than non-users, despite no significant differences in dependency, somatic symptoms, or coping strategies. Conclusions: Employment patterns differed: more users were dedicated to household tasks, while more non-users were self-employed, suggesting economic factors may affect app uptake and quality of life perceptions. Findings indicate AppER is a valid, well-rated support tool, and that caregivers with lower perceived quality of life may be more inclined to adopt digital health solutions, potentially to address complex care demands. Designing targeted digital interventions may be particularly valuable for those in less favorable socioeconomic contexts. Because of the small sample and between-group imbalances, results are exploratory and warrant confirmation in larger, balanced samples. Full article

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11 pages, 272 KB

Open AccessArticle

Bayesian Bell Regression Model for Fitting of Overdispersed Count Data with Application

by Ameer Musa Imran Alhseeni and Hossein Bevrani

Stats 2025, 8(4), 95; https://doi.org/10.3390/stats8040095 (registering DOI) - 10 Oct 2025

Abstract

The Bell regression model (BRM) is a statistical model that is often used in the analysis of count data that exhibits overdispersion. In this study, we propose a Bayesian analysis of the BRM and offer a new perspective on its application. Specifically, we [...] Read more.

The Bell regression model (BRM) is a statistical model that is often used in the analysis of count data that exhibits overdispersion. In this study, we propose a Bayesian analysis of the BRM and offer a new perspective on its application. Specifically, we introduce a G-prior distribution for Bayesian inference in BRM, in addition to a flat-normal prior distribution. To compare the performance of the proposed prior distributions, we conduct a simulation study and demonstrate that the G-prior distribution provides superior estimation results for the BRM. Furthermore, we apply the methodology to real data and compare the BRM to the Poisson and negative binomial regression model using various model selection criteria. Our results provide valuable insights into the use of Bayesian methods for estimation and inference of the BRM and highlight the importance of considering the choice of prior distribution in the analysis of count data. Full article

(This article belongs to the Section Computational Statistics)

24 pages, 1149 KB

Open AccessReview

Shaping Architecture with Generative Artificial Intelligence: Deep Learning Models in Architectural Design Workflow

by Socrates Yiannoudes

Architecture 2025, 5(4), 94; https://doi.org/10.3390/architecture5040094 (registering DOI) - 10 Oct 2025

Abstract

Deep-learning generative AI promises to transform architectural design, yet its potential employment and ready-to-use capacity for professional workflows are unclear. This study presents a systematic review conducted in accordance with PRISMA 2020 guidelines, synthesizing peer-reviewed work from 2015 to 2025 to assess how [...] Read more.

Deep-learning generative AI promises to transform architectural design, yet its potential employment and ready-to-use capacity for professional workflows are unclear. This study presents a systematic review conducted in accordance with PRISMA 2020 guidelines, synthesizing peer-reviewed work from 2015 to 2025 to assess how GenAI methods align with architectural practice. A total of 1566 records were initially retrieved across databases, of which 42 studies met eligibility criteria after structured screening and selection. Each was evaluated using five indicators with a three-tier rubric: Output Representation Type, Pipeline Integration, Workflow Standardization, Tool Readiness, and Technical Skillset. Results show that most outputs are raster images or non-editable objects, with only a minority producing CAD/BIM-ready geometry. Workflow pipelines are often fragmented with manual hand-offs and most GenAI methods map only onto the early conceptual design stage. Prototypes frequently require bespoke coding and advanced expertise. These findings indicate a persistent gap between experimentation with ideation-oriented GenAI and the pragmatism of CAD/BIM-centered delivery. By framing the proposed rubric as a workflow maturity model, this review contributes a replicable benchmark for assessing practice readiness and identifying pathways toward mainstream adoption. For GenAI to move from prototypes to mainstream architectural design practice, it is essential to address not only technical barriers, but also cultural issues such as professional skepticism and reliability concerns, as well as ecosystem challenges of data sharing, authorship, and liability. Full article

(This article belongs to the Special Issue Shaping Architecture with Computation)

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11 pages, 855 KB

Open AccessArticle

Estimating Whale Shark, Rhincodon typus, Length Using Multi-Stereo-Image Measurement

by Hiroto Yamamoto, Akira Sasaki, Tomoki Kanna, Yasushi Mitsunaga and Shinsuke Torisawa

Fishes 2025, 10(10), 513; https://doi.org/10.3390/fishes10100513 (registering DOI) - 10 Oct 2025

Abstract

The whale shark Rhincodon typus is the largest known extant omnivorous fish species, reaching up to 17 m in length. Because of its slow growth and late maturity, R. typus is particularly vulnerable to human activities and is listed as endangered on the [...] Read more.

The whale shark Rhincodon typus is the largest known extant omnivorous fish species, reaching up to 17 m in length. Because of its slow growth and late maturity, R. typus is particularly vulnerable to human activities and is listed as endangered on the IUCN Red List. Understanding its biological characteristics, such as growth rate, is essential for their conservation. Non-invasive methods, including stereo-image measurements, have been used to measure the body length of the species over the years, which aggregates in coastal areas during specific life stages. This method enables us to estimate fish length by recording the target using a stereo camera, which commonly consists of two cameras. However, measurement errors increase in the setup as the target moves away from the camera. Therefore, we conducted a multi-stereo video shoot of a free-swimming whale shark in an aquarium tank and compared the performance of stereo cameras using two, three, and four cameras. The setups with three and four cameras outperformed the traditional two-camera stereo setup in terms of precision and accuracy, suggesting that a multi-stereo camera system can effectively estimate the body length of large animals such as whale sharks from a considerable distance. Full article

(This article belongs to the Special Issue New Technologies for Improving Fisheries and Aquaculture Production and Management)

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