MDPI - Publisher of Open Access Journals

11 pages, 1143 KB

Open AccessCommunication

Development of Nanobody-Based Sandwich ELISA Resistant to SpA Interference for Sensitive Detection of Staphylococcal Enterotoxin A

by Chenghao Hu, Di Wang, Yangwei Ou, Ruoyu Li, Qi Chen and Peng Liu

Biosensors 2025, 15(10), 666; https://doi.org/10.3390/bios15100666 (registering DOI) - 3 Oct 2025

Abstract

Staphylococcus aureus is a major pathogen responsible for staphylococcal food poisoning (SFP), with its pathogenicity primarily dependent on staphylococcal enterotoxins (SEs). Among these, staphylococcal enterotoxin A (SEA) is a critical risk factor due to its high toxicity, high detection rate (accounting for 80% [...] Read more.

Staphylococcus aureus is a major pathogen responsible for staphylococcal food poisoning (SFP), with its pathogenicity primarily dependent on staphylococcal enterotoxins (SEs). Among these, staphylococcal enterotoxin A (SEA) is a critical risk factor due to its high toxicity, high detection rate (accounting for 80% of SFP cases), strong thermal stability, and resistance to hydrolysis. Traditional SEA immunoassays, such as enzyme-linked immunosorbent assay (ELISA), are prone to false-positive results caused by nonspecific binding interference from S. aureus surface protein A (SpA). In recent years, nanobodies (single-domain heavy-chain antibodies) have emerged as an ideal alternative to address SpA interference owing to their small molecular weight (15 kDa), high affinity, robust stability, and lack of Fc regions. In this study, based on a previously developed highly specific monoclonal antibody against SEA (mAb-4C6), four anti-SEA nanobodies paired with mAb-4C6 were obtained through two-part (four-round) of biopanning from a naive nanobody phage display library. Among these, SEA-4-20 and SEA-4-31 were selected as optimal candidates and paired with mAb-4C6 to construct double-antibody sandwich ELISAs. The detection limits for SEA were 0.135 ng/mL and 0.137 ng/mL, respectively, with effective elimination of SpA interference. This approach provides a reliable tool for rapid and accurate detection of SEA in food, clinical, and environmental samples. Full article

(This article belongs to the Special Issue Immunoassays and Biosensing (2nd Edition))

► Show Figures

Figure 1

13 pages, 7148 KB

Open AccessArticle

Cutting Performance of TiN/DLC-Coated Cemented Carbide Tool in Dry Cutting of Laser-Clad Cr-Ni-Based Steel

by Zixiang Xia, Wenlong Song, Hongjin Yu, Xing Li, Yijia Yin and Weidong Xie

Coatings 2025, 15(10), 1150; https://doi.org/10.3390/coatings15101150 (registering DOI) - 3 Oct 2025

Abstract

To improve the dry-machining performance of a traditional-coated cemented carbide tool when cutting the laser-clad Cr-Ni-based steel, TiN/DLC multilayer coatings were fabricated using physical vapor deposition (PVD). The coated tools were tested for their surface and cross-sectional morphology, roughness, and microhardness. Dry-cutting experiments [...] Read more.

To improve the dry-machining performance of a traditional-coated cemented carbide tool when cutting the laser-clad Cr-Ni-based steel, TiN/DLC multilayer coatings were fabricated using physical vapor deposition (PVD). The coated tools were tested for their surface and cross-sectional morphology, roughness, and microhardness. Dry-cutting experiments were conducted to compare the performance of a TiN monolayer-coated tool and a TiN/DLC multilayer-coated tool. The results indicated that the TiN/DLC multilayer coatings significantly improved the machining performance, lowered the cutting force and cutting temperature, decreased the average friction coefficient at the rake face, and reduced surface roughness compared to the TiN-coated tool. This improvement is mainly attributed to the low shear strength of the DLC layer, which effectively reduces surface friction and wear of the tool. The main failure modes were abrasive wear and adhesive wear. The results suggest that the composite coating offers a promising approach to improving traditional-coated tool life and enhancing machining efficiency in the dry cutting of laser-clad alloy components. Full article

(This article belongs to the Collection Hard Protective Coatings on Tools and Machine Elements)

► Show Figures

Figure 1

12 pages, 991 KB

Open AccessArticle

Associations Between Swimmers’ Dry-Land Lower- and Upper-Limb Measures and Butterfly Sprint Performance

by Maciej Hołub, Wojciech Głyk, Arkadiusz Stanula, Katja Weiss, Thomas Rosemann and Beat Knechtle

Sports 2025, 13(10), 346; https://doi.org/10.3390/sports13100346 (registering DOI) - 3 Oct 2025

Abstract

The aim of the study was to determine correlations between performance of vertical jumps and dolphin kick sprints, and between the results of a dry-land butterfly arm pull test and butterfly arms-only swimming. The study recruited competitive junior male swimmers (15.9 (0.7) years, [...] Read more.

The aim of the study was to determine correlations between performance of vertical jumps and dolphin kick sprints, and between the results of a dry-land butterfly arm pull test and butterfly arms-only swimming. The study recruited competitive junior male swimmers (15.9 (0.7) years, 179.3 (5.3) cm body height, 64.6 (4.3) kg body mass). On dry land, we measured jump height, lower-limb work and power, as well as peak velocity, power, and force in the butterfly arm pull test. In swimming tests, time, velocity, power, force, and work were assessed during the dolphin kick and butterfly arms-only trials. Pearson’s correlation coefficients and the coefficients of determination were calculated between measurements. The findings showed correlations between swimming velocity and power recorded during the dolphin kick test with jump height, work and power measured in the jump tests (maximum r = 0.90, r² = 0,81, p < 0.05). The best correlations between the results of the jump tests and swim variables were determined for the CJ30s test. The butterfly arm pull test was not associated with all parameters measured by the butterfly arms-only test. Our study demonstrates that targeted dry-land training programmes using exercises like vertical jumps can enhance competitive swimmers’ performance and offer coaches an accessible means of tracking athlete progress. Moreover, such simple drills may serve as a cost-effective approach for early evaluation of strength and power potential and for preventing musculoskeletal injuries, all without requiring pool access or specialized underwater equipment. However, the small and homogeneous sample (n = 12, junior males only) and the absence of reliability analyses limit the generalizability of the results. Full article

► Show Figures

Figure 1

23 pages, 15968 KB

Open AccessArticle

YOLOv8n-RMB: UAV Imagery Rubber Milk Bowl Detection Model for Autonomous Robots’ Natural Latex Harvest

by Yunfan Wang, Lin Yang, Pengze Zhong, Xin Yang, Chuanchuan Su, Yi Zhang and Aamir Hussain

Agriculture 2025, 15(19), 2075; https://doi.org/10.3390/agriculture15192075 (registering DOI) - 3 Oct 2025

Abstract

Natural latex harvest is pushing the boundaries of unmanned agricultural production in rubber milk collection via integrated robots in hilly and mountainous regions, such as the fixed and mobile tapping robots widely deployed in forests. As there are bad working conditions and complex [...] Read more.

Natural latex harvest is pushing the boundaries of unmanned agricultural production in rubber milk collection via integrated robots in hilly and mountainous regions, such as the fixed and mobile tapping robots widely deployed in forests. As there are bad working conditions and complex natural environments surrounding rubber trees, the real-time and precision assessment of rubber milk yield status has emerged as a key requirement for improving the efficiency and autonomous management of these kinds of large-scale automatic tapping robots. However, traditional manual rubber milk yield status detection methods are limited in their ability to operate effectively under conditions involving complex terrain, dense forest backgrounds, irregular surface geometries of rubber milk, and the frequent occlusion of rubber milk bowls (RMBs) by vegetation. To address this issue, this study presents an unmanned aerial vehicle (UAV) imagery rubber milk yield state detection method, termed YOLOv8n-RMB, in unstructured field environments instead of manual watching. The proposed method improved the original YOLOv8n by integrating structural enhancements across the backbone, neck, and head components of the network. First, a receptive field attention convolution (RFACONV) module is embedded within the backbone to improve the model’s ability to extract target-relevant features in visually complex environments. Second, within the neck structure, a bidirectional feature pyramid network (BiFPN) is applied to strengthen the fusion of features across multiple spatial scales. Third, in the head, a content-aware dynamic upsampling module of DySample is adopted to enhance the reconstruction of spatial details and the preservation of object boundaries. Finally, the detection framework is integrated with the BoT-SORT tracking algorithm to achieve continuous multi-object association and dynamic state monitoring based on the filling status of RMBs. Experimental evaluation shows that the proposed YOLOv8n-RMB model achieves an AP@0.5 of 94.9%, an AP@0.5:0.95 of 89.7%, a precision of 91.3%, and a recall of 91.9%. Moreover, the performance improves by 2.7%, 2.9%, 3.9%, and 9.7%, compared with the original YOLOv8n. Plus, the total number of parameters is kept within 3.0 million, and the computational cost is limited to 8.3 GFLOPs. This model meets the requirements of yield assessment tasks by conducting computations in resource-limited environments for both fixed and mobile tapping robots in rubber plantations. Full article

(This article belongs to the Special Issue Plant Diagnosis and Monitoring for Agricultural Production)

► Show Figures

Figure 1

18 pages, 1406 KB

Open AccessArticle

The Value of the First Repetition: Force, Impulse, and Linear Velocity in Flywheel Deadlifts and Their Link to Maximal Free-Weight Strength

by Athanasios Tsoukos and Gregory C. Bogdanis

Sports 2025, 13(10), 345; https://doi.org/10.3390/sports13100345 (registering DOI) - 3 Oct 2025

Abstract

The purpose of this study was threefold: (a) to analyze differences in mean force, impulse, mean concentric and eccentric velocity, and peak concentric velocity across six repetitions of the flywheel deadlift exercise, with a particular focus on the first repetition initiated from zero [...] Read more.

The purpose of this study was threefold: (a) to analyze differences in mean force, impulse, mean concentric and eccentric velocity, and peak concentric velocity across six repetitions of the flywheel deadlift exercise, with a particular focus on the first repetition initiated from zero momentum; (b) to explore relationships between these kinetic and kinematic variables and one-repetition maximum (1-RM) performance in the free-weight deadlift; (c) to examine the effects of different flywheel inertial loads on the relationships among mean force (MF), impulse, time under tension (TUT), and velocity, with the aim of identifying the most valid and reliable parameter for flywheel load prescription. Thirteen resistance-trained men (24.7 ± 5.0 y; 82.2 ± 11.7 kg; 1-RM deadlift: 174 ± 24 kg) performed six repetitions of the flywheel deadlift against six inertial loads (0.025 to 0.145 kg∙m²) on a kBox 5 device. Results showed that although the first repetition had 25–30% lower mean concentric velocity and 7–11% lower mean force compared to subsequent repetitions (p < 0.001), it exhibited 4–8% higher impulse due to the 14–20% longer time under tension. MF, velocity, and impulse in the first repetition showed moderate-to-strong correlations with 1-RM (r = 0.58 to 0.85, p < 0.05), particularly at the two higher inertia loads. MF plateaued at moderate inertia loads, while impulse and TUT increased linearly with increasing inertial load and demonstrated the strongest and most consistent relationships with inertial load (r = 0.99 ± 0.01 and 0.97 ± 0.02, p < 0.001), enabling individualized flywheel training prescription. This study highlights the distinct value of the first repetition in flywheel deadlifts and its practical value for both assessment and training. Also, it suggests that impulse and TUT may be used as simple and practical flywheel exercise prescription variables. Full article

(This article belongs to the Special Issue Enhancing Health Through Physical Activity and Sports Science: Innovations in Applied Research)

► Show Figures

Figure 1

20 pages, 3021 KB

Open AccessArticle

Spot Volatility Measurement Using a Change-Point Duration Model in the High-Frequency Market

by Zhicheng Li, Haipeng Xing and Yan Wang

Int. J. Financial Stud. 2025, 13(4), 186; https://doi.org/10.3390/ijfs13040186 (registering DOI) - 3 Oct 2025

Abstract

Modeling high-frequency volatility is an important topic of market microstructure, as it provides the empirical tools to measure and analyze the rapid price movements. Yet, volatility at a high frequency often exhibits abrupt shifts driven by news and trading activity, making accurate estimation [...] Read more.

Modeling high-frequency volatility is an important topic of market microstructure, as it provides the empirical tools to measure and analyze the rapid price movements. Yet, volatility at a high frequency often exhibits abrupt shifts driven by news and trading activity, making accurate estimation challenging. This study develops a change-point duration (CPD) model to estimate spot volatility, in which price-change intensities remain constant between events but may shift at random change points. Using simulations and empirical analysis of Nasdaq limit order book data, we demonstrate that the CPD model achieves a favorable balance between responsiveness to sudden shocks and stability in volatility dynamics. Moreover, it outperforms benchmark approaches, including the classical autoregressive conditional duration model, nonparametric duration-based estimators, and candlestick-based measures. These findings highlight the CPD framework as an effective tool for volatility estimation in high-frequency trading environments. Full article

(This article belongs to the Special Issue Market Microstructure and Liquidity)

► Show Figures

Figure 1

9 pages, 2285 KB

Open AccessCase Report

Recurrent Cutaneous Leiomyosarcoma: A Histopathological Perspective and the Quest for Complete Excision

by Andreea Cătălina Tinca, Bianca Andreea Lazar, Andreea-Raluca Cozac-Szőke and Ovidiu Simion Cotoi

Dermato 2025, 5(4), 18; https://doi.org/10.3390/dermato5040018 (registering DOI) - 3 Oct 2025

Abstract

Cutaneous leiomyosarcoma is a rare, malignant tumor that arises from smooth muscle cells, accounting for less than 3% of all cutaneous sarcomas. Our case report details a 63-year-old male patient who presented with a rapidly growing, painful nodule in the popliteal region. The [...] Read more.

Cutaneous leiomyosarcoma is a rare, malignant tumor that arises from smooth muscle cells, accounting for less than 3% of all cutaneous sarcomas. Our case report details a 63-year-old male patient who presented with a rapidly growing, painful nodule in the popliteal region. The patient underwent initial surgical excision in September 2021, followed by three subsequent resections until March 2022 due to local recurrence. Histopathological analysis of all specimens revealed a dermal neoplasm composed of spindle cells arranged in intersecting fascicles with storiform patterns. The immunohistochemistry profile showed strong positivity for the markers SMA and desmin, confirming the diagnosis. Despite early interventions, the deep surgical margins were positive, and further surgeries were required until tumor-free margins were achieved. This case emphasizes the morphological characteristics, clinical behavior, and therapeutic challenges in managing cutaneous leiomyosarcoma. A favorable prognosis is achieved with long-term follow-up and a multidisciplinary approach. Full article

(This article belongs to the Special Issue What Is Your Diagnosis?—Case Report Collection)

► Show Figures

Figure 1

4 pages, 133 KB

Open AccessEditorial

Underwater Acoustic Technologies for Sustainable Fisheries

by Jianfeng Tong, Yong Tang and Tohru Mukai

Fishes 2025, 10(10), 497; https://doi.org/10.3390/fishes10100497 (registering DOI) - 3 Oct 2025

Abstract

Underwater acoustic technologies have emerged as indispensable tools for advancing research, management, and conservation across aquatic ecosystems [...] Full article

(This article belongs to the Special Issue Underwater Acoustic Technologies for Sustainable Fisheries)

12 pages, 662 KB

Open AccessArticle

Accuracy of Patient Setup Using Surface Guided Radiotherapy (SGRT) for Abdominal Malignancies

by Varvara Sotiropoulou, Stefanos Kachris and Michalis Mazonakis

Methods Protoc. 2025, 8(5), 119; https://doi.org/10.3390/mps8050119 (registering DOI) - 3 Oct 2025

Abstract

This study aimed to evaluate the placement accuracy and reproducibility of Surface Guided Radiotherapy (SGRT) compared with the conventional tattoo/laser method in patients undergoing radiotherapy for abdominal malignancies. A retrospective analysis was conducted on 43 patients treated with either SGRT (Group A) or [...] Read more.

This study aimed to evaluate the placement accuracy and reproducibility of Surface Guided Radiotherapy (SGRT) compared with the conventional tattoo/laser method in patients undergoing radiotherapy for abdominal malignancies. A retrospective analysis was conducted on 43 patients treated with either SGRT (Group A) or the tattoo/laser technique (Group B). Patients in both groups underwent CBCT to quantify the positioning shifts in the vertical (Svrt), lateral (Slat) and longitudinal (Slng) axes, as well as the total shift. Statistical indicators including median, interquartile range (IQR), and range were calculated, and Mann–Whitney U tests were performed due to non-normal data distribution. Median values in all axes were same between groups: Svrt = 0.4 cm, Slat = 0.2 cm, Slng = 0.4 cm. Group A showed a higher total median shift equal to 0.8 cm versus 0.7 cm of Group B. However, IQRs were smaller in the Group B for all directions and total shift, indicating greater method consistency. Statistically significant differences (p < 0.05) were observed in all axes, except the vertical. These findings suggest that, while SGRT achieves comparable median alignment, its use in a highly variable anatomical region such as the abdomen may be associated with greater setup variability. Full article

(This article belongs to the Section Biomedical Sciences and Physiology)

► Show Figures

Figure 1

4 pages, 150 KB

Open AccessEditorial

Sustainable Innovations in Shrimp Aquaculture: Current Advances and Future Horizons

by Wujie Xu

Fishes 2025, 10(10), 498; https://doi.org/10.3390/fishes10100498 (registering DOI) - 3 Oct 2025

Abstract

With global seafood demand projected to increase by 38% by 2050 [...] Full article

(This article belongs to the Special Issue Advances in Shrimp Aquaculture: Management and Sustainability)

22 pages, 4284 KB

Open AccessArticle

Research on Denoising Methods for Magnetocardiography Signals in a Non-Magnetic Shielding Environment

by Biao Xing, Xie Feng and Binzhen Zhang

Sensors 2025, 25(19), 6096; https://doi.org/10.3390/s25196096 (registering DOI) - 3 Oct 2025

Abstract

Magnetocardiography (MCG) offers a noninvasive method for early screening and precise localization of cardiovascular diseases by measuring picotesla-level weak magnetic fields induced by cardiac electrical activity. However, in unshielded magnetic environments, geomagnetic disturbances, power-frequency electromagnetic interference, and physiological/motion artifacts can significantly overwhelm effective [...] Read more.

Magnetocardiography (MCG) offers a noninvasive method for early screening and precise localization of cardiovascular diseases by measuring picotesla-level weak magnetic fields induced by cardiac electrical activity. However, in unshielded magnetic environments, geomagnetic disturbances, power-frequency electromagnetic interference, and physiological/motion artifacts can significantly overwhelm effective magnetocardiographic components. To address this challenge, this paper systematically constructs an integrated denoising framework, termed “AOA-VMD-WT”. In this approach, the Arithmetic Optimization Algorithm (AOA) adaptively optimizes the key parameters (decomposition level K and penalty factor α) of Variational Mode Decomposition (VMD). The decomposed components are then regularized based on their modal center frequencies: components with frequencies ≥50 Hz are directly suppressed; those with frequencies <50 Hz undergo wavelet threshold (WT) denoising; and those with frequencies <0.5 Hz undergo baseline correction. The purified signal is subsequently reconstructed. For quantitative evaluation, we designed performance indicators including QRS amplitude retention rate, high/low frequency suppression amount, and spectral entropy. Further comparisons are made with baseline methods such as FIR and wavelet soft/hard thresholds. Experimental results on multiple sets of measured MCG data demonstrate that the proposed method achieves an average improvement of approximately 8–15 dB in high-frequency suppression, 2–8 dB in low-frequency suppression, and a decrease in spectral entropy ranging from 0.1 to 0.6 without compromising QRS amplitude. Additionally, the parameter optimization exhibits high stability. These findings suggest that the proposed framework provides engineerable algorithmic support for stable MCG measurement in ordinary clinic scenarios. Full article

(This article belongs to the Section Biomedical Sensors)

► Show Figures

Figure 1

15 pages, 1053 KB

Open AccessReview

Vernix Caseosa Peritonitis: A Scoping Review

by Goran Augustin, Mislav Herman, Zrinka Hrgović, Ante Krešo and Jure Krstulović

Medicina 2025, 61(10), 1786; https://doi.org/10.3390/medicina61101786 (registering DOI) - 3 Oct 2025

Abstract

Background and Objectives: Vernix caseosa peritonitis (VCP) is rare. Nonspecific symptoms of acute abdomen during early puerperium make preoperative diagnosis of VCP challenging. We aimed to identify risk factors, early diagnosis and treatment options, and the association between the timing and severity [...] Read more.

Background and Objectives: Vernix caseosa peritonitis (VCP) is rare. Nonspecific symptoms of acute abdomen during early puerperium make preoperative diagnosis of VCP challenging. We aimed to identify risk factors, early diagnosis and treatment options, and the association between the timing and severity of VCP diagnosis and maternal outcomes. Materials and Methods: We searched PubMed, PubMed Central, and Google Scholar. Articles were analyzed according to the PRISMA guidelines. The search items included: ‘vernix caseosa peritonitis, ‘vernix caseosa granuloma, ‘maternal meconium peritonitis’, ‘maternal meconium granuloma’, ‘vernix caseosa’, ‘peritonitis’, ‘pregnancy’, ‘puerperium’, ‘postpartum’, and ‘gravid’. Additional studies were extracted by reviewing the reference lists of retrieved studies. Demographic, clinical, obstetric, diagnostic, and treatment parameters, and outcomes were collected. Results: Out of 55 published VCP case reports, 46 were available. Most involved term pregnancies (84.8%) and were delivered by Cesarean section (CS) (87%), with no difference in parity distribution (χ²(2) = 1.1875, p = 0.5523) or fetal sex (m: f = 53.3%: 46.7%). Common symptoms included abdominal pain and fever over 38 °C, while dyspnea or tachypnea was unexpectedly frequent (23.9%/15.2%). The interval from delivery to surgery ranged from 4 to 13 days (average 8 days), with no difference between CS and vaginal deliveries. Preoperative VCP was diagnosed in only 4.3% of cases, and intraoperative diagnosis occurred in 60.9%. Intraoperative microbiology and histopathology (vernix components) were positive in 6.5% and 89.1%, respectively. The birth weight was normal (3656 ± 509 g), with no maternal or neonatal deaths. Conclusions: VCP primarily develops in term pregnancies delivered by CS, without other risk factors. Despite extremely low preoperative and unexpectedly low intraoperative diagnosis and treatment delay of several days, there is no maternal or fetal mortality. The time to symptom onset is similar between women who delivered vaginally and those who had a CS. All women with VCP after vaginal delivery had previous deliveries. Abdominal pain with a fever over 38 °C and dyspnea or tachypnea in the early puerperium suggests VCP. Surgical lavage is the primary treatment, while corticosteroids have been reported to be beneficial in several cases, and antibiotics seem to have a limited role. Full article

(This article belongs to the Section Obstetrics and Gynecology)

► Show Figures

Figure 1

25 pages, 1745 KB

Open AccessArticle

On the Practical Philosophy of the Nuns’ Buddhist Academy at Mount Wutai Through “One-Week Intensive Buddha Retreats”

by Yong Li, Yi Zhang and Jing Wang

Religions 2025, 16(10), 1267; https://doi.org/10.3390/rel16101267 (registering DOI) - 3 Oct 2025

Abstract

The educational philosophy of the Nuns’ Buddhist Academy at Pushou Monastery, Mount Wutai, is based on the principles of “Hua Yan as the foundation, precepts as the practice, and Pure Land as the destination.” This philosophy draws upon Buddhist scriptures, integrating descriptions of [...] Read more.

The educational philosophy of the Nuns’ Buddhist Academy at Pushou Monastery, Mount Wutai, is based on the principles of “Hua Yan as the foundation, precepts as the practice, and Pure Land as the destination.” This philosophy draws upon Buddhist scriptures, integrating descriptions of the Pure Land practice found in the Avatamsaka Sūtra and the Amitābha Sūtra. This approach translates the textual teachings of Buddhist classics into real-life practice, expressing the concept of “the non-obstruction of principle and phenomenon” in the tangible activities of practitioners. It also allows for the experiential understanding of the spiritual realms revealed in the scriptures during theoretical learning and practice. The philosophy of the Nuns’ Academy embodies the practical emphasis of Chinese Buddhism, guiding all aspects of learning and practice. This paper argues that the pure land practice is living. In order to understand pure land practice, there should be a comprehensive viewpoint. It is needed to explore this way of practice through the analysis of textual analysis, figuring its root in Buddhis sūtra, as well as a sociological method to investigate its manifestation at the present society. Moreover, the spiritual dimension should not be neglected for a full-scale study. In this sense, the pure land school is living at present. Full article

► Show Figures

Figure 1

19 pages, 6403 KB

Open AccessArticle

Membrane Composition Modulates Vp54 Binding: A Combined Experimental and Computational Study

by Wenhan Guo, Rui Dong, Ayoyinka O. Okedigba, Jason E. Sanchez, Irina V. Agarkova, Elea-Maria Abisamra, Andrew Jelinsky, Wayne Riekhof, Laila Noor, David D. Dunigan, James L. Van Etten, Daniel G. S. Capelluto, Chuan Xiao and Lin Li

Pathogens 2025, 14(10), 1000; https://doi.org/10.3390/pathogens14101000 (registering DOI) - 3 Oct 2025

Abstract

The recruitment of peripheral membrane proteins is tightly regulated by membrane lipid composition and local electrostatic microenvironments. Our experimental observations revealed that Vp54, a viral matrix protein, exhibited preferential binding to lipid bilayers enriched in anionic lipids such as phosphatidylglycerol (PG) and phosphatidylserine [...] Read more.

The recruitment of peripheral membrane proteins is tightly regulated by membrane lipid composition and local electrostatic microenvironments. Our experimental observations revealed that Vp54, a viral matrix protein, exhibited preferential binding to lipid bilayers enriched in anionic lipids such as phosphatidylglycerol (PG) and phosphatidylserine (PS), compared to neutral phosphatidylcholine/phosphatidylethanolamine liposomes, and this occurred in a curvature-dependent manner. To elucidate the molecular basis of this selective interaction, we performed a series of computational analyses including helical wheel projection, electrostatic potential calculations, electric field lines simulations, and electrostatic force analysis. Our results showed that the membrane-proximal region of Vp54 adopted an amphipathic α-helical structure with a positively charged interface. In membranes containing PG or PS, electrostatic potentials at the interface were significantly more negative, enhancing attraction with Vp54. Field line and force analyses further confirmed that both the presence and spatial clustering of anionic lipids intensify membrane–Vp54 electrostatic interactions. These computational findings align with experimental binding data, jointly demonstrating that membrane lipid composition and organization critically modulate Vp54 recruitment. Together, our findings highlight the importance of electrostatic complementarity and membrane heterogeneity in peripheral protein targeting and provide a framework applicable to broader classes of membrane-binding proteins. Full article

► Show Figures

Graphical abstract

16 pages, 491 KB

Open AccessArticle

Lactate Thresholds and Performance in Young Cross-Country Skiers Before and After the Competitive Season: Insights from Laboratory Roller-Ski Tests in Normoxic and Hypoxic Conditions

by Jesús Torres-Pérez, Eneko Fernández-Peña, Alexa Callovini and Aitor Pinedo-Jauregi

Sports 2025, 13(10), 344; https://doi.org/10.3390/sports13100344 (registering DOI) - 3 Oct 2025

Abstract

Cross-country (XC) skiing imposes high physiological demands under hypoxic conditions at altitude. Lactate thresholds such as Onset Blood Lactate Accumulation at 4 mmol/L (OBLA4) and lactate plus 1 mmol/L above baseline (Bsln+1.0) are crucial for tracking performance. This study investigates physiological responses in [...] Read more.

Cross-country (XC) skiing imposes high physiological demands under hypoxic conditions at altitude. Lactate thresholds such as Onset Blood Lactate Accumulation at 4 mmol/L (OBLA4) and lactate plus 1 mmol/L above baseline (Bsln+1.0) are crucial for tracking performance. This study investigates physiological responses in junior XC skiers under normoxic and hypoxic conditions before (PreCs) and after (PosCs) the competitive season. Nine national-level XC skiers performed a Graded Exercise Test (GXT) on a treadmill using roller skis under both normoxic and hypoxic conditions in PreCS and PosCS. Heart rate, slope (treadmill inclination), and lactate thresholds (Bsln+1.0 and OBLA4) were measured. Significant differences were found between PreCs and PosCs under hypoxia for maximum heart rate (p < 0.05). Estimated slopes at Bsln+1.0 and OBLA4 were lower under hypoxia compared to normoxia in PreCs (p = 0.005, d = −1.29 for Bsln+1.0 and p = 0.013, d = −1.06 for OBLA4). In PosCs, a lower impairment effect of hypoxia exposure under slope at OBLA4 was found (p = 0.02, d = −0.95). Positive correlations were found between heart rate and slope for Bsln+1.0 and OBLA4 in PreCs under normoxia and hypoxia, becoming stronger at PosCs, especially under hypoxia. Delta values showed that the higher the slope at Bsln+1.0 and OBLA 4 under normoxia was, the greater the decrease between normoxia and hypoxia was. Physiological changes in junior XC skiers after training and competition in normoxic and hypoxic conditions highlight the importance of hypoxic environments for assessing and monitoring performance throughout the season. Full article

► Show Figures

Figure 1

23 pages, 4359 KB

Open AccessArticle

Use of Inertial Measurement Units for Detection of the Support Phases in Discus Throwing

by José Sánchez-Moreno, David Moreno-Salinas and Juan Carlos Álvarez-Ortiz

Sensors 2025, 25(19), 6095; https://doi.org/10.3390/s25196095 (registering DOI) - 3 Oct 2025

Abstract

Photogrammetry applied to sports provides precise data on athlete positions and time instants, especially with digital motion capture systems. However, detecting and identifying specific events in athletic movements such as discus throwing can be challenging when using only images. For example, with high-speed [...] Read more.

Photogrammetry applied to sports provides precise data on athlete positions and time instants, especially with digital motion capture systems. However, detecting and identifying specific events in athletic movements such as discus throwing can be challenging when using only images. For example, with high-speed video, it is difficult to pinpoint the exact frame when events like foot touchdown or takeoff occur, as contact between shoe and ground may span several frames. Inertial measurement units (IMUs) can detect maxima and minima in linear accelerations and angular velocities, helping to accurately determine these specific events in throwing movements. As a result, comparing photogrammetry data with IMU data becomes challenging because of the differences in the methods used to detect events. Even if comparisons can be made with IMU data from other sports researchers, variations in methodologies can invalidate the comparison. To address this, the paper proposes a simple methodology for detecting the five phases of a discus throw using three IMUs located on the thrower’s wrist and on the instep or ankle of the feet. Experiments with three elite male discus throwers are conducted and the results are compared with existing data in the literature. The findings demonstrate that the proposed methodology is effective (100% of phases detected in the experiments without false positives) and reliable (results validated with professional coaches), offering a practical and time- and cost-effective solution for accurately detecting key moments in athletic movements. Full article

(This article belongs to the Special Issue Sports Sensors for Athlete Motion Tracking and Physiological Monitoring: Second Edition)

► Show Figures

Figure 1

20 pages, 6167 KB

Open AccessArticle

ICU Readmission and In-Hospital Mortality Rates for Patients Discharged from the ICU—Risk Factors and Validation of a New Predictive Model: The Worse Outcome Score (WOScore)

by Eleftherios Papadakis, Athanasia Proklou, Sofia Kokkini, Ioanna Papakitsou, Ioannis Konstantinou, Aggeliki Konstantinidi, Georgios Prinianakis, Stergios Intzes, Marianthi Symeonidou and Eumorfia Kondili

J. Pers. Med. 2025, 15(10), 479; https://doi.org/10.3390/jpm15100479 (registering DOI) - 3 Oct 2025

Abstract

Background: Intensive Care Unit (ICU) readmission and in-hospital mortality are critical indicators of patient outcomes following ICU discharge. Patients readmitted to the ICU often face worse prognosis, higher healthcare costs, and prolonged hospital stays. Identifying high-risk patients is essential for optimizing post-ICU [...] Read more.

Background: Intensive Care Unit (ICU) readmission and in-hospital mortality are critical indicators of patient outcomes following ICU discharge. Patients readmitted to the ICU often face worse prognosis, higher healthcare costs, and prolonged hospital stays. Identifying high-risk patients is essential for optimizing post-ICU care and resource allocation. Methods: This two-phase study included the following: (1) a retrospective analysis of ICU survivors in a mixed medical–surgical ICU to identify risk factors associated with ICU readmission and in-hospital mortality, and (2) a prospective validation of a newly developed predictive model: the Worse Outcome Score (WOScore). Data collected included demographics, ICU admission characteristics, severity scores (SAPS II, SAPS III, APACHE II, SOFA), interventions, complications and discharge parameters. Results: Among 1.190 ICU survivors, 126 (10.6%) were readmitted to the ICU, and 192 (16.1%) died in hospital after ICU discharge. Key risk factors for ICU readmission included Diabetes Mellitus, SAPS III on admission, and ICU-acquired infections (Ventilator-Associated Pneumonia (VAP) and Catheter-Related Bloodstream Infection, (CRBSI)). Predictors of in-hospital mortality were identified: medical admission, high SAPS III score, high lactate level on ICU admission, tracheostomy, reduced GCS at discharge, blood transfusion, CRBSI, and Acute Kidney Injury (AKI) during ICU stay. The WOScore, developed based on the results above, demonstrated strong predictive ability (AUC: 0.845 derivation, 0.886 validation). A cut-off of 20 distinguished high-risk patients (sensitivity: 88.1%, specificity: 73.0%). Conclusions: ICU readmission and in-hospital mortality are influenced by patient severity, underlying comorbidities, and ICU-related complications. The WOScore provides an effective, easy-to-use risk stratification tool that can guide clinicians in identifying high-risk patients at ICU discharge and guide post-ICU interventions, potentially improving patients’ outcomes and optimizing resource allocation. Further multi-center studies are necessary to validate the model in diverse healthcare settings. Full article

(This article belongs to the Section Personalized Medical Care)

► Show Figures

Figure 1

9 pages, 4015 KB

Open AccessCase Report

A Rare Case Presentation of Intraoral Palatal Myoepithelioma

by Abdullah Saeidi, Albraa Alolayan, Hattan Zaki, Emad Essa, Shadi Alzahrani, Wamiq Fareed and Shadia Elsayed

Reports 2025, 8(4), 196; https://doi.org/10.3390/reports8040196 (registering DOI) - 3 Oct 2025

Abstract

Background and Clinical Significance: Palatal swellings may originate from various pathological disorders. These swellings may include congenital or acquired factors. The posterior hard palate, which contains many minor salivary glands, is a common site for such swellings. Case Presentation: We present a rare [...] Read more.

Background and Clinical Significance: Palatal swellings may originate from various pathological disorders. These swellings may include congenital or acquired factors. The posterior hard palate, which contains many minor salivary glands, is a common site for such swellings. Case Presentation: We present a rare case of intraoral palatal myoepithelioma in a 45-year-old Egyptian male with a significant history of smoking. Detailed clinical, radiographic, and operative findings are discussed alongside histopathological evaluation, surgical management, and postoperative outcomes. This case highlights the importance of considering myoepithelioma lesions in the differential diagnosis of posterior palatal swelling. Conclusions: Palatal myoepithelioma is a rare but important benign salivary gland tumor that may resemble multiple other intraoral lesions. A complete clinical, radiographic, and histological investigation is required for a definitive diagnosis. Complete surgical excision achieved a favorable outcome. Increased awareness and reporting of this unusual pathology are critical for deepening knowledge and guiding clinical decisions. Full article

► Show Figures

Figure 1

14 pages, 44018 KB

Open AccessArticle

Arc Fault Detection for Photovoltaic Systems Using Independent Component Analysis Technique and Dynamic Time-Warping Algorithm

by Jiazi Xu, Shuo Ding, Guoli Li and Qunjing Wang

Sensors 2025, 25(19), 6094; https://doi.org/10.3390/s25196094 (registering DOI) - 3 Oct 2025

Abstract

Arc fault detection in photovoltaic systems is crucial, since it may cause incidents like fires and explosions. So far, most existing methods rely on an arc’s local features and do not characterize arc faults globally, which may lead to detection failure in noisy [...] Read more.

Arc fault detection in photovoltaic systems is crucial, since it may cause incidents like fires and explosions. So far, most existing methods rely on an arc’s local features and do not characterize arc faults globally, which may lead to detection failure in noisy environments. In this paper, a fundamentally different method is proposed that relies on an arc’s global features instead of local ones. The core idea of the method is that the physical mechanisms of the arc fault signals and the normal signals are so different that they are thought to be generated by two independent sources. Based on this insight, independent component analysis (ICA) is introduced to decompose the photovoltaic system’s DC currents. By using ICA, the DC current signals with an arc fault can be decomposed into two independent signals, while the normal signals without an arc fault cannot be decomposed into two such independent signals. This indicates that arc faults can be detected by using the concept of “independence”. Then, the dynamic time warping algorithm was used to determine the independence level of the ICA outputs so as to realize end-to-end arc fault detection. Experimental results showed that our method has better performance than traditional methods in terms of detection accuracy and robustness against environmental disturbances. Full article

(This article belongs to the Special Issue Recent Progress on Sensors in Power Industry: System, Signal Processing, and Data Analysis)

► Show Figures

Figure 1

44 pages, 80926 KB

Open AccessArticle

Spatial Organization Patterns and Their Impact on Evacuation Efficiency: Evidence from Primary School Teaching Buildings

by Sen Cao, Wenjia Liu and Jiantao Zhang

Buildings 2025, 15(19), 3560; https://doi.org/10.3390/buildings15193560 - 2 Oct 2025

Abstract

Primary school teaching buildings represent a typical category of densely populated public architecture, where the safe evacuation of occupants is essential to ensuring their safety. The spatial organizational structure plays a pivotal role in determining overall evacuation efficiency. However, systematic research linking spatial [...] Read more.

Primary school teaching buildings represent a typical category of densely populated public architecture, where the safe evacuation of occupants is essential to ensuring their safety. The spatial organizational structure plays a pivotal role in determining overall evacuation efficiency. However, systematic research linking spatial organization with evacuation performance remains limited. This study addresses this gap by analyzing 102 real-world cases of primary school teaching buildings, identifying common spatial organizational patterns, and developing a spatial structural framework based on fundamental units and their organizational relationships. A hybrid methodology integrating weighted network analysis and evacuation simulation is employed to quantitatively evaluate the relationship between spatial organization types and evacuation performance, ultimately proposing three design principles—Integrity, Balance, and Stability—to guide evacuation efficiency optimization. The findings provide a methodological reference for evacuation research in public buildings and offer practical design guidance for optimizing primary school facility layouts. Full article

(This article belongs to the Section Building Energy, Physics, Environment, and Systems)

► Show Figures

Figure 1

13 pages, 1595 KB

Open AccessArticle

Involvement of Vitamin D Receptor Gene Polymorphism in Increased Cardiovascular Risk Disease in the Algerian Population

by Assia Galleze, Fatma Zohra Djaballah-Ider, Ines Gouaref, Sara Mimi Atmani, Karima Allal, Chafia Touil-Boukoffa and Houda Belguendouz

Int. J. Mol. Sci. 2025, 26(19), 9627; https://doi.org/10.3390/ijms26199627 (registering DOI) - 2 Oct 2025

Abstract

Cardiovascular diseases (CVDs) cover various pathologies including heart failure (HF). Furthermore, vitamin D is involved in the regulation of the cardiovascular system. This study aimed to assess the association between the vitamin D receptor (VDR) genotypes and the occurrence of cardiovascular disorders in [...] Read more.

Cardiovascular diseases (CVDs) cover various pathologies including heart failure (HF). Furthermore, vitamin D is involved in the regulation of the cardiovascular system. This study aimed to assess the association between the vitamin D receptor (VDR) genotypes and the occurrence of cardiovascular disorders in the Algerian population. VDR gene polymorphisms were identified using the PCR-RFLP method. Moreover, plasma concentrations of 25-hydroxyvitamin-D were assessed by a chemiluminescent immunoassay method and plasma NT-proBNP levels were determined in vitro by immunoenzymatic analysis. Interestingly, our results indicate that the genotypic frequencies of ApaI polymorphism of the VDR gene were significantly higher in CVD patients compared to the control group. Moreover, higher numbers of AA genotypes and A alleles were found in the CVD group. Our data indicate that the group of CVD patients with HF compared to those without HF showed the same genotype and allele distribution. Furthermore, low vitamin D rates and high N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels according to the VDR rs7975232 genotype were noted in CVD patients compared to healthy controls. Our results indicate that ApaI polymorphism of the VDR gene and lower vitamin D level may be associated with increased cardiovascular risk. These findings indicate that the ApaI AA genotype could be considered as a new HF risk marker in the Algerian population. Full article

(This article belongs to the Section Molecular Genetics and Genomics)

► Show Figures

Figure 1

15 pages, 26587 KB

Open AccessArticle

Effects of a Natural Polyherbal Extract on Alleviating Scopolamine-Induced Memory Deficits in C57BL/6 Mice via Enhancing Cholinergic Function

by Hyeokjin Kwon, Min Ho Kwon, Myeongguk Jeong, Yeeun Kim, Hae-Gyung Yoon, Yeongdon Ju, Kyung-Yae Hyun and Go-Eun Choi

Curr. Issues Mol. Biol. 2025, 47(10), 817; https://doi.org/10.3390/cimb47100817 - 2 Oct 2025

Abstract

Alzheimer’s disease (AD) is a progressive neurological condition with limited effective pharmaceutical treatments, often accompanied by side effects. This has increased interest in plant-based alternatives. This study examined the cognitive effects of a Natural Polyherbal Extract (NPX) on scopolamine-induced memory deficits in mice. [...] Read more.

Alzheimer’s disease (AD) is a progressive neurological condition with limited effective pharmaceutical treatments, often accompanied by side effects. This has increased interest in plant-based alternatives. This study examined the cognitive effects of a Natural Polyherbal Extract (NPX) on scopolamine-induced memory deficits in mice. Male C57BL/6 mice (10 weeks old, n = 36) were divided into four groups: control (saline), scopolamine (1 mg/kg, i.p.), tacrine (10 mg/kg, oral), and NPX (1000 mg/kg, oral). NPX and tacrine were administered daily by oral gavage for two weeks. Cognitive function was assessed weekly using the Y-maze task. Brain tissues were collected for biochemical analysis, including AChE activity and immunohistochemical detection of neurodegeneration-related markers. Results: Mice treated with NPX demonstrated improved spontaneous alternation behavior compared to the scopolamine group. NPX also significantly reduced acetylcholinesterase activity. Immunohistochemistry revealed decreased expression of amyloid-beta (Aβ) and caspase-3, with enhanced choline acetyltransferase levels. These outcomes were comparable to those observed in the tacrine-treated group. Conclusions: NPX alleviated scopolamine-induced memory impairment through enhancement of cholinergic signaling and mitigation of neurodegenerative markers. The findings suggest that NPX may serve as a promising plant-derived candidate for managing memory-related disorders, including AD. Full article

(This article belongs to the Special Issue Advances in Bioinformatics and Molecular Research on Alzheimer’s Disease)

► Show Figures

Figure 1

20 pages, 2995 KB

Open AccessArticle

Investigating the Preferences for Hospital Landscape Design: Results of a Pilot Study from Poland

by Monika Trojanowska, Joanna Matuszewska and Maciej Brosz

Architecture 2025, 5(4), 91; https://doi.org/10.3390/architecture5040091 - 2 Oct 2025

Abstract

One of the sometimes-neglected fields is the landscape design of hospital premises. This study focuses on the perception and preferences of responders regarding hospital site design. The objective was to determine if people are aware of the benefits of restorative contact with nature [...] Read more.

One of the sometimes-neglected fields is the landscape design of hospital premises. This study focuses on the perception and preferences of responders regarding hospital site design. The objective was to determine if people are aware of the benefits of restorative contact with nature and if there were preferences for any specific landscape type. The online questionnaire with color figures was distributed using emails and social media from 4th May to 2nd August 2024. Some 110 respondents returned the questionnaire. Most of the respondents were women under 25. Most respondents declared that the surroundings of the healthcare building influence the health and well-being of patients (96%) and health personnel (86%). The results confirmed the awareness of the importance of contact with nature (89%). Moreover, this study demonstrated a preference for calm garden compositions, stimulating physical and mental recovery with trees, flowers, and water features, as well as stabilized paths and sheltered sitting places. The results confirm previous studies and demonstrate the importance of landscape architecture design of hospital premises for the well-being of patients. The findings may influence urban landscape planning and the design of hospital sites. Full article

21 pages, 469 KB

Open AccessArticle

Development and Content Validation of a Person-Centered Care Instrument for Healthcare Providers

by Krishan Soriano, Sora Nakatani, Kaito Onishi, Hirokazu Ito, Youko Nakano, Yoshiyuki Takashima, Yueren Zhao, Allan Paulo Blaquera, Ryuichi Tanioka, Feni Betriana, Gil Platon Soriano, Yuko Yasahura, Kyoko Osaka, Matsuko Kataoka, Misao Miyagawa, Masashi Akaike, Minoru Irahara and Tetsuya Tanioka

Nurs. Rep. 2025, 15(10), 355; https://doi.org/10.3390/nursrep15100355 - 2 Oct 2025

Abstract

Background/Objectives: Despite the increasing recognition of person-centered care (PCC), existing evaluation tools often have profession-specific limitations, lacking broad applicability across interdisciplinary contexts. This study aimed to develop and validate the Person-Centered Care Instrument (PCCI), designed to assess the competence of healthcare providers [...] Read more.

Background/Objectives: Despite the increasing recognition of person-centered care (PCC), existing evaluation tools often have profession-specific limitations, lacking broad applicability across interdisciplinary contexts. This study aimed to develop and validate the Person-Centered Care Instrument (PCCI), designed to assess the competence of healthcare providers from diverse professions. Methods: Using a two-round modified Delphi technique, ten experts validated an initial pool of 63 items. The process assessed both face validity (overall appropriateness) and content validity using a 9-point Likert scale and the Item-level Content Validity Index (I-CVI). Items with a median rating of 6 or higher and an I-CVI of ≥0.70 were retained. Results: The final PCCI consists of 37 items, with a scale-level content validity index of 0.65. Three items achieved universal agreement among the experts (I-CVI = 1.0). For the final 37-item PCCI, the Scale-level Content Validity Index (S-CVI) was 0.65, and the index based on universal agreement was 0.22. Conclusions: The developed PCCI demonstrated good face and content validity, making it a valid and broadly applicable tool for assessing competence in delivering PCC. This instrument can support quality improvement initiatives and help promote a culture of empathy and respect in healthcare. Full article

► Show Figures

Figure 1

18 pages, 1628 KB

Open AccessPatent Summary

Manual Resin Gear Drive for Fine Adjustment of Schlieren Optical Elements

by Emilia Georgiana Prisăcariu and Iulian Vlăducă

Inventions 2025, 10(5), 89; https://doi.org/10.3390/inventions10050089 - 2 Oct 2025

Abstract

High-precision angular positioning mechanisms are essential across diverse scientific and industrial applications, from optical instrumentation to automated mechanical systems. Conventional bronze–steel gear reduction units, while reliable, are often heavy, costly, and unsuitable for chemically aggressive or vacuum environments, limiting their use in advanced [...] Read more.

High-precision angular positioning mechanisms are essential across diverse scientific and industrial applications, from optical instrumentation to automated mechanical systems. Conventional bronze–steel gear reduction units, while reliable, are often heavy, costly, and unsuitable for chemically aggressive or vacuum environments, limiting their use in advanced research setups. This work introduces a novel 1:360 gear reduction system manufactured by resin-based additive manufacturing, designed to overcome these limitations. The compact worm–gear assembly translates a single crank rotation into a precise one-degree indicator displacement, enabling fine and repeatable angular control. A primary application is the alignment of parabolic mirrors in schlieren systems, where accurate tilt adjustment is critical to correct optical alignment; however, the design is broadly adaptable to other precision positioning tasks in laboratory and industrial contexts. Compared with conventional assemblies, the resin-based reducer offers reduced weight, chemical and vacuum compatibility, and lower production cost. Its three-stage reduction design further enhances load-bearing capacity, achieving approximately double the theoretical torque transfer of equivalent commercial systems. These features establish the device as a robust, scalable, and automation-ready solution for high-accuracy angular adjustment, contributing both to specialized optical research and general-purpose precision engineering. Full article

(This article belongs to the Section Inventions and Innovation in Advanced Manufacturing)

13 pages, 2617 KB

Open AccessArticle

Kilowatt-Level EUV Regenerative Amplifier Free-Electron Laser Enabled by Transverse Gradient Undulator in a Storage Ring

by Changchao He, Nanshun Huang, Tao Liu, Changliang Li, Bo Liu and Haixiao Deng

Photonics 2025, 12(10), 983; https://doi.org/10.3390/photonics12100983 - 2 Oct 2025

Abstract

High-average-power extreme ultraviolet (EUV) sources are essential for large-scale nanoscale chip manufacturing, yet commercially available laser-produced plasma sources face challenges in scaling to the kilowatt level. We propose a novel scheme that combines the high repetition rate of a diffraction-limited storage ring with [...] Read more.

High-average-power extreme ultraviolet (EUV) sources are essential for large-scale nanoscale chip manufacturing, yet commercially available laser-produced plasma sources face challenges in scaling to the kilowatt level. We propose a novel scheme that combines the high repetition rate of a diffraction-limited storage ring with a regenerative amplifier free-electron laser (RAFEL) employing a transverse gradient undulator (TGU). By introducing dispersion in the storage ring, electrons of different energies are directed into corresponding magnetic field strengths of the TGU, thereby satisfying the resonance condition under a large energy spread and increasing the FEL gain. Simulations show that at equilibrium, the average EUV power exceeds 1 kW, with an output pulse energy reaching ∼2.86

μ

J, while the energy spread stabilizes at ∼0.45%. These results demonstrate the feasibility of ring-based RAFEL with TGU as a promising route toward kilowatt-level EUV sources. Full article

(This article belongs to the Special Issue Next-Generation X-Ray Optical Technologies and Applications)

20 pages, 703 KB

Open AccessArticle

Fast Trace Detection of Chlorpyrifos Vapors Using a Handheld Ion Mobility Spectrometer Operated near Ambient Temperature

by Victor Bocoș-Bințințan, Ancuța-Maria Dodea, Tomáš Rozsypal, Adrian Pătruț, Gheorghe Roșian, Aurel-Vasile Martiniuc, Alin-Gabriel Moraru, Simina Vasc and Maria-Paula Bocoș-Bințințan

Toxics 2025, 13(10), 843; https://doi.org/10.3390/toxics13100843 - 2 Oct 2025

Abstract

Chlorpyrifos CPF (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate), known also as Chlorpyrifos-ethyl, is one of the most utilized organophosphorus pesticides worldwide. Additionally, CPF could be used as a chemical warfare agent surrogate. Although its acute toxicity is not high, it is responsible for both a large [...] Read more.

Chlorpyrifos CPF (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate), known also as Chlorpyrifos-ethyl, is one of the most utilized organophosphorus pesticides worldwide. Additionally, CPF could be used as a chemical warfare agent surrogate. Although its acute toxicity is not high, it is responsible for both a large number of intoxications and chronic, delayed neurological effects. In this work, it is reported for the first time the qualitative and quantitative response produced by CPF vapors, using a pocket-held Time-of-Flight Ion Mobility Spectrometer (ToF IMS) with a non-radioactive ionization source and ammonia doping, model LCD-3.2E (Smiths Detection Ltd.), operated near ambient temperature (below 30 °C). Spectra of CPF in positive ion mode included two distinct product ion peaks; thus, identification of CPF vapors by IMS relies on these peaks—the monomer M·NH₄⁺ with reduced ion mobility K₀ = ca. 1.76 cm²V⁻¹s⁻¹ and the dimer M₂·NH₄⁺ with K₀ = ca. 1.47 cm²V⁻¹s⁻¹ (where M may be assignable to CPF molecule)—and positive reactant ions (Pos RIP) have K₀ = ca. 2.25 cm²V⁻¹s⁻¹. Excellent sensitivity, with a limit of detection LOD of 0.72 ppb_v (10.5 μg m⁻³) and a limit of quantification LOQ of 2.41 ppb_v (35.1 μg m⁻³), has been noticed; linear response was up to 100 ppb_v, while saturation occurs over ca. 1000 ppb_v (14.6 mg m⁻³). Our results demonstrate that this method provides a robust tool for both off-site and on-site detecting and quantifying CPF vapors at trace levels, which has strong implications for either industrial hygiene or forensic investigations concerning the pesticide Chlorpyrifos, as well as for monitoring of environmental contamination by organophosphorus pesticides. Full article

(This article belongs to the Section Agrochemicals and Food Toxicology)

► Show Figures

Graphical abstract

Recent Articles

Open Access Journals

Highly Accessed Articles

Latest Books

News

Blog Posts

Topics

Selected Special Issues

Selected Collections

Journals by Subject

Further Information

Guidelines

MDPI Initiatives

Follow MDPI