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16 pages, 1384 KB

Open AccessArticle

Intravenous Lidocaine for Postoperative Pain and Recovery After Robotic Prostate Adenomectomy: A Retrospective Observational Cohort Study

by Georgiana Maria Popa, Simona-Alina Abu-Awwad, Ahmed Abu-Awwad, Carmen-Ioana Marta, Erika Bimbo-Szuhai, Mihaela Gabriela Bontea, Adrian Gheorghe Osiceanu, Anca Mihaela Bina, Cristian Mihai Moisa Cezar, Ciprian Dumitru Puscas and Mihai O. Botea

Medicina 2025, 61(11), 2045; https://doi.org/10.3390/medicina61112045 (registering DOI) - 16 Nov 2025

Abstract

Background and Objectives: Effective perioperative pain management remains a key goal of enhanced recovery protocols, especially in minimally invasive urologic surgery, where optimizing comfort while limiting opioid exposure is essential. Intravenous lidocaine has gained attention for its multimodal analgesic and anti-inflammatory properties, [...] Read more.

Background and Objectives: Effective perioperative pain management remains a key goal of enhanced recovery protocols, especially in minimally invasive urologic surgery, where optimizing comfort while limiting opioid exposure is essential. Intravenous lidocaine has gained attention for its multimodal analgesic and anti-inflammatory properties, yet evidence in robotic prostatectomy remains limited. This study evaluated whether intraoperative lidocaine infusion was associated with lower early postoperative pain scores and reduced opioid use in patients undergoing robotic-assisted radical prostatectomy. Materials and Methods: A retrospective, single-center analysis was conducted at Pelican Clinical Hospital, Oradea, Romania, including 112 patients operated on between January 2020 and December 2023. All procedures were performed by the same surgical and anesthetic teams using standardized ERAS-based protocols. Patients were divided into two groups: the Lidocaine Group (LG, n = 51), who received a bolus of 1.5 mg/kg lidocaine followed by an infusion of 1.5 mg/kg/h during surgery, and the Control Group (CG, n = 61), who received standard anesthesia without lidocaine. Postoperative pain was measured using the visual analog scale (VAS) at 0, 4, 12, and 24 h, and opioid use was converted into morphine milligram equivalents (MME). Secondary outcomes included time to ambulation, gastrointestinal recovery, oral intake, hospital stay, and complications. Results: Pain intensity was significantly lower in the lidocaine group at 4 h postoperatively (VAS 3.5 ± 1.1 vs. 4.3 ± 1.3; p = 0.01), with similar scores later. Total opioid use was reduced by about 18% in the lidocaine group (25.7 ± 9.4 vs. 31.2 ± 10.5 MME; p = 0.03). Recovery parameters and complication rates were comparable between groups, and no lidocaine-related adverse events were recorded. Conclusions: Intraoperative intravenous lidocaine was associated with lower early postoperative pain scores and reduced opioid requirements after robotic-assisted radical prostatectomy without affecting recovery or safety. Its favorable profile and low cost support its inclusion as a practical adjunct in multimodal analgesia within ERAS pathways. Full article

(This article belongs to the Special Issue Anesthesiology, Resuscitation, and Pain Management)

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15 pages, 1098 KB

Open AccessArticle

Quantifying Bilateral Synchrony and Asymmetry of Neurovascular Responses to Post-Occlusive Reactive Hyperemia

by Henrique Silva, Nicole Lavrador and Hugo Alexandre Ferreira

Appl. Sci. 2025, 15(22), 12142; https://doi.org/10.3390/app152212142 (registering DOI) - 16 Nov 2025

Abstract

Post-occlusive reactive hyperemia (PORH) is widely used to assess microvascular reactivity, but its systemic impact on contralateral neurovascular function remains unclear. This study quantified bilateral synchrony and asymmetry of cutaneous signals during unilateral PORH in healthy subjects using a novel multidimensional framework of [...] Read more.

Post-occlusive reactive hyperemia (PORH) is widely used to assess microvascular reactivity, but its systemic impact on contralateral neurovascular function remains unclear. This study quantified bilateral synchrony and asymmetry of cutaneous signals during unilateral PORH in healthy subjects using a novel multidimensional framework of inter-limb coherence. Twelve young adults underwent a standard suprasystolic occlusion (5 min at 200 mmHg) on the upper limb, while photoplethysmography (PPG), skin temperature, and electrodermal activity (EDA) were recorded bilaterally in the fingers. Coherence was characterized by profile similarity (Cross-Signal Similarity Index, CSSI), temporal lag (τ*), magnitude asymmetry (Bilateral Magnitude Difference Index, BDMI), directional concordance (Signal Direction Index, SDI; Directional Concordance Index, DCI), and integrated indices (IBIL, IBIS). At baseline, all signals showed high bilateral synchrony (CSSI ≈ 0.9; τ* < 20 ms). Occlusion markedly reduced CSSI for blood flow (0.89 to 0.07, p = 0.002) and temperature (0.93 to −0.03, p = 0.06), while EDA coherence remained preserved (0.95 to 0.82). Integrated indices decreased significantly (IBIL 0.84 to 0.17, p = 0.005; IBIS 0.84 to 0.18, p = 0.004) and recovered only partially during hyperemia (IBIL 0.20, p = 0.003). Directional concordance was heterogeneous: during hyperemia, 9 of 12 subjects showed concordant EDA changes but only 7 of 12 for perfusion. BDMI was largest for perfusion (≈0.8), moderate for temperature (≈0.5), and minimal for EDA (≈0.3). Unilateral PORH thus induces a marked loss of bilateral coherence in microvascular signals, whereas sympathetic-driven responses remain strongly synchronized. This dissociation reveals that occlusion evokes systemic autonomic adjustments beyond local hemodynamics. The proposed framework captures hidden aspects of neurovascular integration and may provide new markers for autonomic imbalance or perfusion asymmetry. Full article

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24 pages, 3294 KB

Open AccessArticle

Ultrasound-Assisted Fibril Formation Enhances Complexation of Oat Globulin with Quercetin: Mechanism, Structure Evolution, Delivery Performance

by Jinzhao Xu, Xiao Zhao and Qingfeng Ban

Foods 2025, 14(22), 3916; https://doi.org/10.3390/foods14223916 (registering DOI) - 16 Nov 2025

Abstract

Amyloid fibrillization represents an effective strategy for extending and enhancing protein function, particularly for the delivery of hydrophobic active substances. In this study, oat globulin (OG) and its fibrils were complexed with quercetin (Que) to construct the delivery system, and ultrasonic pretreatment was [...] Read more.

Amyloid fibrillization represents an effective strategy for extending and enhancing protein function, particularly for the delivery of hydrophobic active substances. In this study, oat globulin (OG) and its fibrils were complexed with quercetin (Que) to construct the delivery system, and ultrasonic pretreatment was applied during fibril preparation to explore the promoter of complex formation. The results demonstrated that complexation with Que induced a dose-dependent static quenching of the intrinsic fluorescence of the protein/fibrils, with hydrophobic interactions and tryptophan residues being the primary interaction forces and the main fluorescence quenching groups, respectively. In comparison, OG fibrils prepared with ultrasound pretreatment (UOGF) exhibited the strongest encapsulation and loading capacity for Que, ranging from 97.16% at a mass ratio of 200:1 to 42.48% at a ratio of 25:1. Subsequently, complexes were prepared with a ratio of 50:1. Structural analysis revealed that Que primarily interacts with the protein/fibril carriers through hydrogen bonds and hydrophobic interactions, inducing structural changes and ultimately being encapsulated in an amorphous form within the composite material. Additionally, Que promoted the mutual aggregation and cross-linking of protein/fibril units, leading to increased hydrodynamic diameter and zeta-potential. Moreover, UOGF-Que showed the greatest improvement in the thermal stability and the photostability of Que, and enhancing the bioaccessibility. These findings provide valuable insights into using ultrasound as an auxiliary measure for fibril self-assembly to enhance the application potential of fibrils, especially the delivery of hydrophobic functional substances. Full article

(This article belongs to the Special Issue Effect of the Application of Novel Processes to Food Proteins on Structure, Physicochemical Properties and Modifications)

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12 pages, 2151 KB

Open AccessArticle

An Astaxanthin-Binding Ependymin-Related Purple Protein Responsible for the Coloration of a Marine Purple Sponge, Haliclona sp.

by Takayuki Kaneko, Tomomi Asano and Shinji Kawasaki

Mar. Drugs 2025, 23(11), 441; https://doi.org/10.3390/md23110441 (registering DOI) - 16 Nov 2025

Abstract

Although marine sponges display strikingly diverse colors, the molecular basis of this color diversity remains largely unknown. Recently, the blue coloration of Haliclona sp. was attributed to a water-soluble carotenoprotein that binds orange astaxanthin (AXT) and mytiloxanthin (MXT) and belongs to the ependymin [...] Read more.

Although marine sponges display strikingly diverse colors, the molecular basis of this color diversity remains largely unknown. Recently, the blue coloration of Haliclona sp. was attributed to a water-soluble carotenoprotein that binds orange astaxanthin (AXT) and mytiloxanthin (MXT) and belongs to the ependymin superfamily. Here, we investigated the coloration mechanism of a purple sponge, Haliclona sp. The purified purple protein was identified as a secreted glycoprotein, representing the second example of a color protein belonging to the ependymin superfamily. The blue and purple proteins were accordingly designated carotenoependymin (Cep)-Blue1 and Cep-Purple1. Cep-Blue1 binds orange AXT and MXT in a 1:1 ratio, whereas Cep-Purple1 binds only AXT, producing a smaller red shift than Cep-Blue1 in the 550–750 nm range. In vitro reconstitution of carotenoid-free apoproteins with their native carotenoids reproduced the original spectra. When the carotenoids bound to Cep-Blue1 and Cep-Purple1 were exchanged and reconstituted in vitro, Cep-Blue1 reconstituted with AXT exhibited a purplish-blue color, whereas Cep-Purple1 reconstituted with an equimolar mixture of AXT and MXT showed a preference for AXT and displayed an incomplete red shift. These results suggest that the subtle color variations among Haliclona species are determined by both species-specific carotenoid composition and the structural features of carotenoependymin proteins. Full article

(This article belongs to the Special Issue Marine Carotenoids: Properties, Health Benefits, and Applications)

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17 pages, 931 KB

Open AccessReview

The Effect of Maternal Stress on 11beta-Hydroxysteroid Dehydrogenase Activity During Pregnancy: Evidence for Potential Pregnancy Complications and Consequences on Fetal Development and Metabolism

by Polina Pavli, George Mastorakos, Makarios Eleftheriades and Georgios Valsamakis

Int. J. Mol. Sci. 2025, 26(22), 11071; https://doi.org/10.3390/ijms262211071 (registering DOI) - 16 Nov 2025

Abstract

Τhe intrauterine environment has a strong connection with the growing fetus and possible effects that can continue up to adulthood. Currently, stress is conceptualized as a modern teratogen. The overwhelming majority of studies indicate that maternal stress during pregnancy may have effects on [...] Read more.

Τhe intrauterine environment has a strong connection with the growing fetus and possible effects that can continue up to adulthood. Currently, stress is conceptualized as a modern teratogen. The overwhelming majority of studies indicate that maternal stress during pregnancy may have effects on pregnancy outcomes and fetal development, with long-lasting consequences on child and adult vulnerability to disease. Glucocorticoids are essential for regulating fetal development, growth, and metabolism. The two isoforms of 11beta-hydroxysteroid dehydrogenase enzyme (11β-HSD) mediate and regulate glucocorticoid actions and biological activity. It has not yet been fully elucidated whether maternal stress during pregnancy affects 11β-HSD isoenzyme activity and expression and results in possible adverse effects on fetal development, metabolism, and pregnancy outcomes. This review examines a possible pathophysiological mechanism by which maternal stress during pregnancy affects placental 11β-HSD isoenzyme activity, thereby causing adverse effects on the physiological status of pregnancy, fetal development, and metabolism. Furthermore, the main outcome of the review is the following: chronic and acute maternal stress during pregnancy affects the activity and the expression of placental 11β-HSD isoenzymes and has possible subsequent unfavorable results on preeclampsia, preterm birth, and fetuses with intrauterine growth restriction (IUGR) or small for gestational age (SGA) fetuses. Full article

(This article belongs to the Special Issue Endocrinology of Pregnancy)

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15 pages, 16847 KB

Open AccessArticle

High-Power Laser Coherent Beam Combination Through Self-Imaging in Plasma Waveguides

by Yixuan Huang, Haitao Zhang, Zhuoyi Yang, Yanwei Wang, Yihang Huang, Xiaozheng Liu and Junyu Chen

Appl. Sci. 2025, 15(22), 12141; https://doi.org/10.3390/app152212141 (registering DOI) - 16 Nov 2025

Abstract

A novel approach for laser coherent beam combination (CBC) utilizing the self-imaging effect in plasma waveguides is presented in this study, which enables the transmission of ultrashort laser pulses at intensities above the bulk damage threshold of conventional solid optical waveguides. The feasibility [...] Read more.

A novel approach for laser coherent beam combination (CBC) utilizing the self-imaging effect in plasma waveguides is presented in this study, which enables the transmission of ultrashort laser pulses at intensities above the bulk damage threshold of conventional solid optical waveguides. The feasibility of self-imaging-based CBC in plasma waveguides was simulated and verified, demonstrating favorable combining efficiency and beam quality. This work explores the adaptive tuning of waveguide length via dynamic adjustment of plasma density, addressing the critical issue of fabrication tolerances in traditional waveguide systems. With CBC via plasma waveguide, this study offers support for the development of robust, high-power laser systems with enhanced beam quality and operational stability. Full article

(This article belongs to the Special Issue Advances in Fiber Lasers and Their Applications)

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16 pages, 1370 KB

Open AccessArticle

Evaluation of Immune Response Dynamics: Analyzing the Parameters of Complete Blood Count (CBC) in Experimental Borreliosis

by Diana Mihaela Alexandru, Diana Larisa Ancuţa and Cristin Coman

Life 2025, 15(11), 1758; https://doi.org/10.3390/life15111758 (registering DOI) - 16 Nov 2025

Abstract

The spirochete Borrelia is responsible for Lyme disease, a multisystemic infection and growing public health concern. This study aimed to evaluate host response dynamics to Borrelia bavariensis by analyzing hematological parameters as potential immuno-inflammatory markers in a murine model. Forty C3He/HeNCrl mice were [...] Read more.

The spirochete Borrelia is responsible for Lyme disease, a multisystemic infection and growing public health concern. This study aimed to evaluate host response dynamics to Borrelia bavariensis by analyzing hematological parameters as potential immuno-inflammatory markers in a murine model. Forty C3He/HeNCrl mice were inoculated intradermally with B. bavariensis (5 × 10⁵ spirochetes/100 µL/mouse) and monitored for 90 days. Samples were collected at defined intervals for microbiological examination, hematology, and qPCR. Microbiological and qPCR testing revealed infection between days 7–21; results were negative on days 28–42. At later stages (days 60 and 90), Borrelia was only detectable by qPCR, highlighting differences in diagnostic sensitivity. Hematological analysis showed that the neutrophil-to-lymphocyte ratio (NLR) and systemic immuno-inflammatory index (SII) peaked on day 7 (p < 0.0001), followed by gradual normalization until day 35. These markers reflected the intensity of the inflammatory response and defined three distinct phases of host reaction. Overall, results demonstrate the complexity of immune responses in B. bavariensis infection and underscore the value of monitoring hematological indices for understanding host–pathogen interactions. This approach supports the potential use of simple blood markers in diagnostic strategies with translational relevance for clinical practice. Full article

(This article belongs to the Special Issue From Animal Models to Clinical Innovations: Translating Research into Medicine)

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17 pages, 2257 KB

Open AccessArticle

Determination of UAV Flight Altitude and Time for Optimizing Variable-Rate Nitrogen Prescription Maps for Winter Wheat in the North China Plain

by Minne Zhang, Weixia Zhao and Jiusheng Li

Agronomy 2025, 15(11), 2627; https://doi.org/10.3390/agronomy15112627 (registering DOI) - 16 Nov 2025

Abstract

An unmanned aerial vehicle (UAV) multi-spectral system provides a monitoring platform to rapidly obtain crop spectral information that can reflect crop nitrogen status for the generation of dynamic variable-rate nitrogen (VRN). To improve the accuracy of VRN prescription maps, a method of generating [...] Read more.

An unmanned aerial vehicle (UAV) multi-spectral system provides a monitoring platform to rapidly obtain crop spectral information that can reflect crop nitrogen status for the generation of dynamic variable-rate nitrogen (VRN). To improve the accuracy of VRN prescription maps, a method of generating VRN prescription maps on the basis of the vegetation index was proposed, and the effects of UAV flight time and altitude on VRN prescription maps were analyzed. The experimental site was located in Dacaozhuang, Hebei Province, China, and the experimental crop was winter wheat (Lunxuan 145). The flight altitudes of the UAV system were set to 50, 70 and 90 m. The flight times were set to 8:00 a.m., 11:00 a.m., 2:00 p.m. and 5:00 p.m. local time. The flight area was 1.18 ha with a 60° rotation angle under a three-span center pivot irrigation system with an overhang. UAV flight missions were executed during the jointing, heading, and grain filling phases of winter wheat. There were 90 management zones with pie shapes in total, which were composed of a 10° angle in the rotation direction and 4 sprinklers along the lateral direction. The vegetation indices (VIs) which are closely related to crop nutrient status were selected and used to generate distribution maps, which were superimposed with the management zones to generate VRN prescription maps. The results demonstrated that the red-edge soil adjusted vegetation index (RESAVI) was relatively more sensitive to the nitrogen status of winter wheat than the other VIs were. The RESAVI distributions were stable during periods with a solar elevation angle greater than 50° (11:00 a.m.–2:00 p.m. local time), and the VRN prescription maps were similar, with the overlap percentage of the same fertilization grade being greater than 80% and the relative error of the fertilization amount being less than 5%. Compared with that at 2:00 p.m., the overlap percentage of the same fertilization grade was 56.6% in both seasons at 8:00 a.m., whereas flights at 5:00 p.m. exhibited overlaps of 70.9% and 44.6% in the 2023 and 2024 seasons, respectively. Conversely, the flight altitude had little influence on the fertilizer amount and VRN prescription maps. The difference in the amount of fertilizer used was less than 3% at different flight altitudes. The required time is half of that for a 50 m flight when the flight altitude is 70 m and one third of that when the flight altitude is 90 m. Our study recommended operating the UAV multi-spectral system at solar elevation angles greater than 50° when generating VRN prescription maps of winter wheat, and the flight height can be adjusted according to the field area and the endurance time of the UAV. Full article

(This article belongs to the Section Precision and Digital Agriculture)

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21 pages, 23269 KB

Open AccessArticle

Wavelet-Guided Zero-Reference Diffusion for Unsupervised Low-Light Image Enhancement

by Yuting Peng, Xiaojun Guo, Mengxi Xu, Bing Ding, Bei Sun and Shaojing Su

Electronics 2025, 14(22), 4460; https://doi.org/10.3390/electronics14224460 (registering DOI) - 16 Nov 2025

Abstract

Low-light image enhancement (LLIE) remains a challenging task due to the scarcity of paired training data and the complex signal-dependent noise inherent in low-light scenes. To address these issues, this paper proposes a fully unsupervised framework named Wavelet-Guided Zero-Reference Diffusion (WZD) for natural [...] Read more.

Low-light image enhancement (LLIE) remains a challenging task due to the scarcity of paired training data and the complex signal-dependent noise inherent in low-light scenes. To address these issues, this paper proposes a fully unsupervised framework named Wavelet-Guided Zero-Reference Diffusion (WZD) for natural low-light image restoration. WZD leverages an ImageNet-pre-trained diffusion prior and a multi-scale representation of the Discrete Wavelet Transform (DWT) to restore natural illumination from a single dark image. Specifically, the input low-light image is first processed by a Practical Exposure Corrector (PEC) to provide an initial robust luminance baseline. It is then converted from the RGB to the YCbCr color space. The Y channels of the input image and the current diffusion estimate are decomposed into four orthogonal sub-bands—LL, LH, HL, and HH—and fused via learnable, step-wise weights while preserving structural integrity. An exposure control loss and a detail consistency loss are jointly employed to suppress over/under-exposure and preserve high-frequency details. Unlike recent approaches that rely on complex supervised training or lack physical guidance, our method integrates wavelet guidance with a zero-reference learning framework, incorporates the PEC module as a physical prior, and achieves significant improvements in detail preservation and noise suppression without requiring paired training data. Comprehensive experiments on the LOL-v1, LOL-v2, and LSRW datasets demonstrate that WZD achieves a superior or competitive performance, surpassing all referenced unsupervised methods. Ablation studies confirm the critical roles of the PEC prior, YCbCr conversion, wavelet-guided fusion, and the joint loss function. WZD also enhances the performance of downstream tasks, verifying its practical value. Full article

(This article belongs to the Topic International Conference on Autonomous Unmanned Systems (5th ICAUS 2025))

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11 pages, 1210 KB

Open AccessArticle

A Class-F High-Power Rectifier Circuit Based on Admittance Matching

by Xiao-Ning Li, Long-Feng Tian, Lin-Feng Du, Tong Wu, Guang-Chuan Zhai and Yong-Qiang Chen

Electronics 2025, 14(22), 4461; https://doi.org/10.3390/electronics14224461 (registering DOI) - 16 Nov 2025

Abstract

This paper proposes a novel high-efficiency microwave rectifier circuit with a three-stage harmonic control network based on admittance matching technology. The microwave rectifier circuit is mainly composed of a three-stage Class-F harmonic control network, an admittance matching structure, and a DC filtering structure. [...] Read more.

This paper proposes a novel high-efficiency microwave rectifier circuit with a three-stage harmonic control network based on admittance matching technology. The microwave rectifier circuit is mainly composed of a three-stage Class-F harmonic control network, an admittance matching structure, and a DC filtering structure. The three-stage Class-F harmonic control network, featuring a simple structure, not only achieves the control of the second and third harmonics but also performs impedance control on the fourth harmonic to further improve efficiency, while also realizing the impedance matching function. The DC filtering structure eliminates traditional LC components to reduce losses; meanwhile, it uses fan-shaped microstrip lines to achieve filtering and completes admittance matching with the three-stage harmonic control network. This paper presents the simulation, fabrication and measurement of a high-power rectifier circuit. The results of the measurement show that at 2.55 GHz, with an input power of 32.5 dBm, the rectifier circuit achieves a maximum rectification efficiency of 76.9%, exhibiting excellent high-power performance. Additionally, it addresses the difficulty of impedance-matching technology being relatively complex for parallel circuits. The use of admittance for matching provides valuable reference significance for reducing the complexity of parallel circuit matching and enhancing the intuitiveness of matching in related research. Full article

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5 pages, 159 KB

Open AccessEditorial

Innovative Design and Application of Modern Agricultural Machinery Systems in Cropping Systems

by Chung-Liang Chang and Mustafa Ucgul

Agriculture 2025, 15(22), 2371; https://doi.org/10.3390/agriculture15222371 (registering DOI) - 16 Nov 2025

Abstract

The recent increase in extreme weather events and the variety of crop types and planting patterns have shifted agricultural machinery research toward achieving consistent, high-quality performance in the field [...] Full article

(This article belongs to the Special Issue Innovative Design and Application of Modern Agricultural Machinery Systems in Cropping Systems)

26 pages, 2147 KB

Open AccessArticle

Differential Features of Cholecystokinin-Releasing Peptides Derived from Food Proteins: Peptide Length, Amino Acid Composition and Primary Structure; Analysis of Currently Identified Peptide Sequences

by Giovanni Tulipano

Int. J. Mol. Sci. 2025, 26(22), 11065; https://doi.org/10.3390/ijms262211065 (registering DOI) - 15 Nov 2025

Abstract

The mouse enteroendocrine cell line STC-1 has been widely used to investigate the effects of dietary protein-derived peptides on cholecystokinin (CCK) secretion. The studies have also addressed the question of whether specific structural features of a given peptide chain may be related to [...] Read more.

The mouse enteroendocrine cell line STC-1 has been widely used to investigate the effects of dietary protein-derived peptides on cholecystokinin (CCK) secretion. The studies have also addressed the question of whether specific structural features of a given peptide chain may be related to higher secretagogue activity with respect to others, but a detailed structure–activity relationship in CCK-releasing peptides has not yet been reported. The aim of this study was to list the currently available CCK-releasing peptide sequences; to draw conclusions about the role played by peptide length, peptide amino acid composition and peptide amino acid sequence in differentiating their secretagogue activity; and to highlight the physicochemical properties and sequence motifs shared by the active peptides, and any possible differential feature between CCK-releasing peptides and ineffective peptides. To this end, a method was applied consisting of the fractionation of peptide sets into subsets and the comparison between paired subsets of active and inactive peptides. A few distinctive structural features related to CCK-releasing activity were highlighted for each subset. Actually, minor changes in the primary structure can make the difference between active and inactive peptides, as suggested by previous studies. Hence, the chance of predicting the activity of a peptide that has never been tested in vitro using reference structures must still be considered to be low. Full article

(This article belongs to the Special Issue Bioactive Peptides: Synthesis, Functions, and Medicinal Chemistry Applications)

19 pages, 1342 KB

Open AccessArticle

Multimodal Rehabilitation in Spinal Cord Lesion: Comparative Outcomes of Vojta Therapy and Lokomat Training

by Anamaria Gherle, Carmen Delia Nistor-Cseppento, Liviu Lazar, Ștefania Deac, Mirela Elena Bodea, Florin Mihai Marcu, Sebastian Tirla and Mariana Lidia Cevei

Medicina 2025, 61(11), 2041; https://doi.org/10.3390/medicina61112041 (registering DOI) - 15 Nov 2025

Abstract

Background and Objectives: Spinal cord lesion is a severe disorder of the central nervous system, leading to partial or complete interruption of nerve impulse transmission between the brain and the periphery and causing severe neurological and functional deficits. Conventional rehabilitation offers limited [...] Read more.

Background and Objectives: Spinal cord lesion is a severe disorder of the central nervous system, leading to partial or complete interruption of nerve impulse transmission between the brain and the periphery and causing severe neurological and functional deficits. Conventional rehabilitation offers limited outcomes, while robotic gait training (Lokomat^®) and Vojta Therapy have shown benefits individually. Evidence on their combined effect remains scarce. To evaluate the combined effect of Vojta Therapy and Lokomat-assisted gait training on motor recovery, functional independence, and quality of life in SCL patients. Materials and Methods: A retrospective clinical study was conducted on 205 patients with traumatic and non-traumatic SCL. Patients were allocated to four groups: (F)—conventional rehabilitation; (V)—conventional + Vojta; (L)—conventional + Lokomat; (VL)—conventional + Vojta + Lokomat. Assessments included the ASIA Impairment Scale (AIS), ASIA motor/sensory scores, spasticity (Modified Ashworth Scale, MAS), functional independence (Functional Independence Measure, FIM), and health-related quality of life (EQ-5D), performed at admission and discharge. Statistical analyses comprised paired t-tests, Wilcoxon signed-rank tests, chi-square tests, Kruskal–Wallis with Dunn’s post hoc corrections, and linear regression. Results: The most frequent lesion levels were C7 (21%) and L1 (20%). All groups showed improvement in FIM scores, with the greatest gains in the VL group (from 79.25 to 84.79, p < 0.05). Post hoc analysis confirmed significantly higher FIM outcomes in VL compared with L. Regression analysis identified the ASIA motor score as the strongest predictor of functional independence (β = 0.76, p < 0.001), with VL group membership adding +10.3 points (p = 0.004). EQ-5D indicated persistent deficits in mobility and self-care, especially in VL patients, consistent with higher lesion severity. Conclusions: Combining Vojta Therapy with Lokomat training provides additional functional benefits compared with Lokomat or Vojta alone. Multimodal individualized rehabilitation appears promising for patients with spinal cord lesions. Prospective randomized controlled trials with long-term follow-up are warranted. Full article

(This article belongs to the Special Issue Clinical Applications of Modern Technologies in Neurosurgery and Spine Surgery)

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13 pages, 844 KB

Open AccessArticle

Triple rAAV9 Vector Combinations Encoding Broadly Neutralizing Antibodies Effectively Suppress HIV-1 Infection in Humanized Mice

by Danila S. Leontyev, Felix A. Urusov, Dina V. Glazkova, Boris V. Belugin, Anastasia A. Mitiushina, Galina M. Tsyganova, Sergey M. Yudin, Elena V. Bogoslovskaya and German A. Shipulin

Int. J. Mol. Sci. 2025, 26(22), 11051; https://doi.org/10.3390/ijms262211051 (registering DOI) - 15 Nov 2025

Abstract

This study investigated the protective efficacy of two distinct combinations of three recombinant adeno-associated virus serotype 9 (rAAV9) vectors encoding broadly neutralizing antibodies against HIV-1—CombiMab-1 and CombiMab-2—in mice humanized with primary CD4⁺ T-lymphocytes. We demonstrated that mice preventively treated with CombiMab-1 or [...] Read more.

This study investigated the protective efficacy of two distinct combinations of three recombinant adeno-associated virus serotype 9 (rAAV9) vectors encoding broadly neutralizing antibodies against HIV-1—CombiMab-1 and CombiMab-2—in mice humanized with primary CD4⁺ T-lymphocytes. We demonstrated that mice preventively treated with CombiMab-1 or CombiMab-2 did not develop viremia and maintained human CD4+ T-lymphocyte counts following viral challenge, in contrast to control animals. These results demonstrate the significant protective capacity of CombiMab-1 and CombiMab-2 against HIV-1 challenge. Full article

(This article belongs to the Special Issue HIV Infection, Pathogenesis and Treatment)

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17 pages, 4449 KB

Open AccessArticle

Effect of Aluminum Content on the Corrosion Behavior of Fe-Mn-Al-C Structural Steels in Marine Environments

by Suotao Wang, Zhidong Sun, Dongjie Li, Qiang Yu and Qingfeng Wang

Metals 2025, 15(11), 1249; https://doi.org/10.3390/met15111249 (registering DOI) - 15 Nov 2025

Abstract

Fe-Mn-Al-C lightweight steel is an alternative to traditional low-alloy structural steels. It is lightweight and can be used to reduce the weight of structures without increasing their density. However, in the marine environment, traditional low-alloy structural steels can be damaged by chloride ions, [...] Read more.

Fe-Mn-Al-C lightweight steel is an alternative to traditional low-alloy structural steels. It is lightweight and can be used to reduce the weight of structures without increasing their density. However, in the marine environment, traditional low-alloy structural steels can be damaged by chloride ions, which shortens their service life. We do not yet understand how aluminum, an important alloying element in lightweight steel, affects the process of corrosion. In this study, we examined Fe-Mn-Al-C lightweight steels with different amounts of aluminum. We used full-immersion simulated marine corrosion tests and multi-dimensional characterization techniques, such as microstructure observation and electrochemical measurements, to explore the relationship between aluminum content and the steel’s corrosion rate, corrosion product structure, and corrosion resistance. The results showed that, compared with CS, the weight loss and rate of corrosion of steels that contain aluminum were a lot lower. While the corrosion rate of CS is approximately 0.068 g·h⁻¹·m⁻², that of 7Al steel is reduced to 0.050 g·h⁻¹·m⁻². The stable phases α-FeOOH and FeAl₂O₄ are formed in the corrosion products when Al is added. As the Al content increases, so does the relative content of these phases. Furthermore, FeAl₂O₄ acts as a nucleation site that refines corrosion product grains, reduces pores and cracks, and significantly improves the compactness of corrosion products. It also forms a dense inner rust layer that blocks the penetration of corrosive ions such as Cl⁻. This study confirmed that aluminum improves the corrosion resistance of steel synergistically by regulating the structure of the corrosion products, optimizing the phase composition, and improving the electrochemical properties. The optimal aluminum content for lightweight steel in marine environments is 7%, within a range of 5–9%. Full article

14 pages, 6209 KB

Open AccessArticle

Biomechanical and Bio-Inspired Perspectives on Root Amputation in Maxillary Molars: An FEA Study

by Öznur Küçük Keleş and Öznur Eraslan

Biomimetics 2025, 10(11), 778; https://doi.org/10.3390/biomimetics10110778 (registering DOI) - 15 Nov 2025

Abstract

This study aimed to evaluate the biomechanics of maxillary first molar teeth following palatal, disto-buccal, and mesio-buccal root amputation. An intact maxillary molar underwent root canal treatment using Reciproc R25 files (VDW, Munich, Germany). The canals were obturated with gutta-percha (DiaDent, Seoul, Republic [...] Read more.

This study aimed to evaluate the biomechanics of maxillary first molar teeth following palatal, disto-buccal, and mesio-buccal root amputation. An intact maxillary molar underwent root canal treatment using Reciproc R25 files (VDW, Munich, Germany). The canals were obturated with gutta-percha (DiaDent, Seoul, Republic of Korea) and 2Seal sealer (VDW, Munich, Germany), and the access cavity was restored with composite resin. A high-resolution CBCT scan of an intact maxillary first molar was obtained using a Planmeca Promax 3D Max system (Planmeca Oy, Helsinki, Finland) at 75 kVp and 10 mA. The acquired data were processed in 3D Slicer software (v5.8.0, BSD license, Boston, MA, USA) to segment enamel, dentin, and pulp based on pixel density variations using the three-point cloud method. A baseline intact model and three root-resected models (palatal, disto-buccal, mesio-buccal) were reconstructed in SolidWorks 2021, with resected roots simulated as being sealed with MTA. Finite element analysis was conducted in CosmosWorks to evaluate von Mises stress distribution under a 300 N static occlusal load. Maximum von Mises stresses were detected at occlusal force application sites. Among root dentin tissues, stress values ranked highest after palatal root resection, followed by the mesio-buccal, disto-buccal, and non-resected models. Conclusions: Palatal root amputation of maxillary first molars generated the highest von Mises stresses in root dentin, suggesting a higher biomechanical risk than disto-buccal or mesio-buccal resections. Full article

(This article belongs to the Section Development of Biomimetic Methodology)

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24 pages, 4646 KB

Open AccessArticle

Experimental Analysis of Granular Flow Behavior for Sustainable Landslide Risk Management and Community Resilience

by Daniel Camilo Roman Quintero, Mauricio Alberto Tapias Camacho and Gustavo Chio Cho

Sustainability 2025, 17(22), 10236; https://doi.org/10.3390/su172210236 (registering DOI) - 15 Nov 2025

Abstract

Sustainable landslide risk management is critical for achieving resilient communities and supporting the United Nations Sustainable Development Goals, particularly in vulnerable mountainous regions of developing countries. This study presents experimental evidence supporting dimensionless analysis approaches for characterizing granular flow behavior, contributing to cost-effective [...] Read more.

Sustainable landslide risk management is critical for achieving resilient communities and supporting the United Nations Sustainable Development Goals, particularly in vulnerable mountainous regions of developing countries. This study presents experimental evidence supporting dimensionless analysis approaches for characterizing granular flow behavior, contributing to cost-effective landslide hazard assessment frameworks. We designed a 4 m experimental flume to investigate the influence of particle characteristics on flow velocity and runout distance, using two materials with contrasting shapes but similar density (~460 kg/m³) and nominal size (~5 mm): uniform crystal beads (φ = 25.2°) and non-uniform crushed granite particles (φ = 36.9°). High-resolution imaging (30 fps, 2336 × 1752 pixels) captured 30 flow experiments from initiation to deposition. Results demonstrate significant differences in flow behavior: crystal beads achieved 50% longer runout distances and 46% higher maximum velocities (380 cm/s vs. 260 cm/s) compared to granite particles. The Savage number (

N_{s a v}

) effectively captured fundamental flow-regime differences, with granite particles exhibiting values seven times lower than crystal beads (3.69 vs. 23.91, p < 0.001), indicating greater frictional energy dissipation relative to collisional energy transfer. The Bagnold number confirmed inertially dominated regimes (

N_{B a g}

> 10⁶) with negligible viscous effects in both materials. These findings demonstrate that accessible material characterization using standard triaxial testing and dimensionless analysis can significantly improve landslide runout prediction accuracy, supporting evidence-based decision-making for sustainable territorial planning and community protection. This research supports the development of practical risk assessment methodologies implementable in resource-limited settings, promoting sustainable development through improved natural hazard management. Full article

(This article belongs to the Section Sustainable Urban and Rural Development)

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17 pages, 2290 KB

Open AccessArticle

Comparative Analysis of Amino Acid, Sugar, Acid and Volatile Compounds in 4-CPA-Treated and Oscillator-Pollinated Cherry Tomato Fruits During Ripe Stage

by Zhimiao Li, Sihui Guan, Meiying Ruan, Zhuping Yao, Chenxu Liu, Hongjian Wan, Qingjing Ye, Yuan Cheng and Rongqing Wang

Foods 2025, 14(22), 3914; https://doi.org/10.3390/foods14223914 (registering DOI) - 15 Nov 2025

Abstract

4-Chlorophenoxyacetic acid (4-CPA) is an auxin-type plant growth regulator widely used in fruit and vegetable production. However, its influence on the nutritional and sensory qualities of horticultural crops remains insufficiently characterized. This study investigated the influence of 4-CPA application and oscillator-mediated pollination on [...] Read more.

4-Chlorophenoxyacetic acid (4-CPA) is an auxin-type plant growth regulator widely used in fruit and vegetable production. However, its influence on the nutritional and sensory qualities of horticultural crops remains insufficiently characterized. This study investigated the influence of 4-CPA application and oscillator-mediated pollination on the metabolic composition of fully ripe fruits of Solanum lycopersicum var. cerasiforme cv. ‘Zheyingfen No. 1’. Two concentrations of 4-CPA (16 mg/L and 8 mg/L) were applied during flowering, and their effects on amino acids, soluble sugars, organic acids, and volatile compounds (VOCs) were comparatively analyzed. The results indicated that treatment with 8 mg/L 4-CPA treatment significantly increased the total amino acid content in ripe fruits compared with the control and the 16 mg/L treatment. Among the 17 amino acids identified, the contents of umami-related amino acids, including glutamic acid (Glu) and aspartic acid (Asp), were markedly enhanced. In particular, Glu content in the C8 treatment was the highest and accounted for more than 50% of the total amino acid content. The accumulation of sugars was not significantly affected by 4-CPA treatment, while the C8 treatment resulted in the lowest level of total organic acids, which are crucial for flavor development at the ripening stage. A 29.35% increase in VOCs was observed” for conciseness in 4-CPA-treated fruits compared with the control. Analysis of relative odor activity values (rOAVs) showed that although 4-CPA treatment reduced the number of aroma-active compounds, it promoted the accumulation of β-ionone, thereby shifting the tomato fruit aroma profile toward floral, woody, sweet, and fruity notes. In summary, 4-CPA treatment regulated the nutritional and flavor quality of ripe cherry tomato fruits by increasing the content of Glu and other amino acids, enhancing the diversity of VOCs, and promoting the formation of key aroma-active substances such as β-ionone. Full article

(This article belongs to the Special Issue Advanced Chromatographic and Spectroscopic Techniques in Food Analysis and Quality Control: 2nd Edition)

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30 pages, 14503 KB

Open AccessArticle

Conjugate Study on Thermal–Hydraulic Performance of Topology-Optimized Lattice-Filled Cooling Channel for Thermal Management of Solid-Oxide Fuel Cells

by Kirttayoth Yeranee, Yuli Cheng and Yu Rao

Energies 2025, 18(22), 6001; https://doi.org/10.3390/en18226001 (registering DOI) - 15 Nov 2025

Abstract

Integrated additional cooling channels offer precise thermal management for solid-oxide fuel cells (SOFCs), mitigating temperature gradients. This research studies the thermal–hydraulic performance of cooling channels integrated between SOFC interconnectors, including a Diamond-type triply periodic minimal surface (TPMS), a conventional topology-optimized structure, and a [...] Read more.

Integrated additional cooling channels offer precise thermal management for solid-oxide fuel cells (SOFCs), mitigating temperature gradients. This research studies the thermal–hydraulic performance of cooling channels integrated between SOFC interconnectors, including a Diamond-type triply periodic minimal surface (TPMS), a conventional topology-optimized structure, and a topology-optimized lattice-filled structure. A conjugate heat transfer analysis is employed to investigate the influences of flow rate within the range of Reynolds numbers from 300 to 5000, and the effects of coolant type, including air and liquid metals, as well as the impacts of structural material. The results demonstrate that the topology-optimized lattice-filled structure, generating high turbulence mixing, achieves superior temperature uniformity, especially at high flow rates, despite having higher thermal resistance and pressure loss than the conventional topology-optimized design. The coolant types show the largest influence on thermal–hydraulic performance, and the use of liquid gallium in the conventional optimized design obtains the best temperature uniformity, yielding differences between the maximum and minimum temperatures of less than 5 K. Moreover, the higher-thermal-conductivity material improves temperature uniformity, even at low flow rates. Overall, the optimized-baffle designs in the conventional topology-optimized model, utilizing high-conductivity coolant and structural materials, could be the most suitable for thermal management of the SOFC. Full article

(This article belongs to the Special Issue Heat Transfer Analysis: Recent Challenges and Applications—2nd Edition)

13 pages, 3020 KB

Open AccessArticle

RrLBD40 Enhances Salt Tolerance in Rosa rugosa via Promoting Root Development

by Mengjuan Bai, Yue Wang, Yuqing Shi, Jianwen Wang and Liguo Feng

Horticulturae 2025, 11(11), 1379; https://doi.org/10.3390/horticulturae11111379 (registering DOI) - 15 Nov 2025

Abstract

LATERAL ORGAN BOUNDARIES DOMAIN (LBD) genes encode plant specific transcription factors that regulate various developmental processes and abiotic stresses. In this study, we characterized RrLBD40 from Rosa rugosa as a nucleus-localized transcription factor harboring a conserved LOB domain. We generated RrLBD40-overexpressing Rosa [...] Read more.

LATERAL ORGAN BOUNDARIES DOMAIN (LBD) genes encode plant specific transcription factors that regulate various developmental processes and abiotic stresses. In this study, we characterized RrLBD40 from Rosa rugosa as a nucleus-localized transcription factor harboring a conserved LOB domain. We generated RrLBD40-overexpressing Rosa rugosa plants and compared them with control plants in terms of physiological indices, root architecture, and Na⁺ homeostasis. The results showed that RrLBD40 overexpression significantly increased peroxidase activity and reduced malondialdehyde content in the roots, indicating enhanced antioxidant capacity under salt stress. Furthermore, RrLBD40 overexpression markedly promoted root growth and development, a similar phenotype consistently observed in RrLBD40 transgenic Arabidopsis plants. Propidium iodide staining and analysis of the Na⁺ flux rates of root tips revealed that the barrier function of the Casparian strip was compromised in both the RrLBD40-overexpression and control plants under salt stress. This disruption of endodermal selectivity permitted Na⁺ influx into the vascular cylinder. Furthermore, neither plants exhibited significant Na⁺ efflux capacity. Taken together, these findings demonstrate that RrLBD40 enhances salt tolerance in Rosa rugosa by primarily promoting root growth and development, rather than modulating Na⁺ homeostasis. Full article

(This article belongs to the Special Issue Stress Response, Development, and Quality Regulation in Horticultural Plants)

18 pages, 1880 KB

Open AccessArticle

Evaluations of Quinone/Hydroquinone Couples Acting as Two Hydrogen Atoms Antioxidants, Radical Quenchers, and Hydrogen Atom Abstractors

by Xiaotang Chen, Jun-Ke Wang, Xiao-Qing Zhu and Guang-Bin Shen

Biomolecules 2025, 15(11), 1606; https://doi.org/10.3390/biom15111606 (registering DOI) - 15 Nov 2025

Abstract

Quinone/hydroquinone couples play a crucial role in a variety of biochemical processes and chemical syntheses. Extending from our previous work, a practical dataset including the thermodynamic driving forces of 12 chemical processes for 118 quinone/hydroquinone couples accepting or releasing two hydrogen atoms in [...] Read more.

Quinone/hydroquinone couples play a crucial role in a variety of biochemical processes and chemical syntheses. Extending from our previous work, a practical dataset including the thermodynamic driving forces of 12 chemical processes for 118 quinone/hydroquinone couples accepting or releasing two hydrogen atoms in DMSO is established. The dataset serves as a foundation for assessing and discussing the thermodynamic capabilities of hydroquinones acting as two-hydrogen-atoms antioxidants or radical quenchers, quinones and semiquinone radicals acting as hydrogen atoms abstractors, and quinone/hydroquinone couples acting as dehydrogenation and hydrogenation reagents. The fundamental thermodynamic knowledge is expected to further promote the broader application of quinone/hydroquinone couples in the field of chemical antioxidation and redox reactions. Full article

(This article belongs to the Section Lipids)

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15 pages, 1496 KB

Open AccessArticle

Assessment of Biodegradable Films as Protective Barriers Toward Sustainable Protection of Coastal Archaeological Sites

by Am Pris John, Sergio Santoro, Efrem Curcio, Pietro Argurio, Francesco Chidichimo, Salvatore Straface and Mauro Francesco La Russa

Sustainability 2025, 17(22), 10237; https://doi.org/10.3390/su172210237 (registering DOI) - 15 Nov 2025

Abstract

Saltwater Intrusion (SWI) is threatening coastal archaeological sites, particularly in Crotone, southern Italy. The study area has been experiencing notable SWI due to over-pumping of groundwater, rising land subsidence, and climate change. Consequently, this study examines the applicability of polycaprolactone (PCL), a common [...] Read more.

Saltwater Intrusion (SWI) is threatening coastal archaeological sites, particularly in Crotone, southern Italy. The study area has been experiencing notable SWI due to over-pumping of groundwater, rising land subsidence, and climate change. Consequently, this study examines the applicability of polycaprolactone (PCL), a common biodegradable polymer, as a protective barrier for archaeological conservation. PCL films were synthesized via solvent casting and dried under controlled conditions. Physicochemical properties of the films were evaluated using six analytical techniques: (1) contact angle measurements for surface hydrophobicity, (2) Fourier-Transform Infrared Spectroscopy (FTIR) for chemical stability, (3) Scanning Electron Microscopy (SEM) for morphological characterization, (4) permeability testing for evaluating saltwater diffusion, (5) mechanical testing for tensile properties, and (6) biodegradability assays for degradation rates. All samples were evaluated at 0, 30, 60, and 90 days in natural seawater. Results from these tests indicate that unmodified PCL films exhibited moderate hydrophobicity, partial hydrolytic degradation, resistance to permeability, declining mechanical strength, and limited biodegradability over the testing period. Full article

(This article belongs to the Topic (World) Heritage Sites and Values in Danger: Climate-Change Related Challenges and Transformation)

26 pages, 659 KB

Open AccessReview

Towards a Research Programme Aiming at Causes and Consequences of Reticulate Evolution

by Christoph Oberprieler

Biology 2025, 14(11), 1601; https://doi.org/10.3390/biology14111601 (registering DOI) - 15 Nov 2025

Abstract

Evolution is reticulate. Reticulation increases diversity and complexity on the different levels of the evolutionary hierarchy. In addition to the tendency for diversity and complexity to increase in unchecked evolutionary systems by ongoing divergence (‘Zero-Force Evolutionary Law’, ‘Biology’s First Law’), reticulate evolution, therefore, [...] Read more.

Evolution is reticulate. Reticulation increases diversity and complexity on the different levels of the evolutionary hierarchy. In addition to the tendency for diversity and complexity to increase in unchecked evolutionary systems by ongoing divergence (‘Zero-Force Evolutionary Law’, ‘Biology’s First Law’), reticulate evolution, therefore, acts as a second mechanism for the establishment of evolutionary novelty and the rise in biodiversity and biocomplexity (‘Biology’s Second Law’). This provides the raw material for subsequent diversity-confining drift and selection processes. In order to fully appreciate reticulation processes as part of an updated paradigm of evolutionary biology, a research programme on the topic should encompass the identification of the fundamental evolutionary entities as vertices and the study of the relationships among these vertices as edges in the resulting network architectures. Additionally, along with surveys on the underlying determinants, this will lead to the study of emergent boundary conditions for reticulations and for the porosity of evolutionary entities. Finally, the programme should address the question whether there are equilibrium conditions between the complete fusion and complete isolation of evolutionary entities (‘Goldilocks Zones’) that foster reticulate evolution. As tools in this research programme, machine learning and modelling approaches, along with methods in the field of network reconstruction, transcriptomics, epigenetics, and karyology, are identified. Full article

(This article belongs to the Section Evolutionary Biology)

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18 pages, 1998 KB

Open AccessArticle

HEXADWSN: Explainable Ensemble Framework for Robust and Energy-Efficient Anomaly Detection in WSNs

by Rahul Mishra, Sudhanshu Kumar Jha, Shiv Prakash and Rajkumar Singh Rathore

Future Internet 2025, 17(11), 520; https://doi.org/10.3390/fi17110520 (registering DOI) - 15 Nov 2025

Abstract

Wireless Sensor Networks (WSNs) have a decisive share in various monitoring and control systems. However, their distributed and resource-constrained nature makes them vulnerable to anomalies caused by factors such as environmental noise, sensor faults, and cyber intrusions. In this paper, HEXADWSN, a hybrid [...] Read more.

Wireless Sensor Networks (WSNs) have a decisive share in various monitoring and control systems. However, their distributed and resource-constrained nature makes them vulnerable to anomalies caused by factors such as environmental noise, sensor faults, and cyber intrusions. In this paper, HEXADWSN, a hybrid ensemble learning-based explainable anomaly detection framework for anomaly detection to improve reliability and interpretability in WSNs, has been proposed. The proposed framework integrates an ensemble learning approach using Autoencoders, Isolation Forests, and One-Class SVMs to achieve robust detection of time-series-based irregularities in the Intel Lab dataset. The framework uses stack and vote ensemble learning. The stack ensemble achieved the highest overall performance, indicating strong effectiveness in detecting varied anomaly patterns. The voting ensemble demonstrated moderate results and offered a balance between detection rate and computation, whereas LSTM, which is efficient at capturing temporal dependencies, exhibited a relatively low performance in the processed dataset. SHAP, LIME, and Permutation Feature Importance techniques are employed for model explainability. These techniques offer insights into feature relevance and anomalies at global and local levels. The framework also measures the mean energy consumption for anomalous and normal data. The interpretability results identified that temperature, humidity, and voltage are the most influential features. HEXADWSN establishes a scalable and explainable foundation for anomaly detection in WSNs, striking a balance between accuracy, interpretability, and energy management insights. Full article

(This article belongs to the Special Issue Wireless Sensor Networks and Internet of Things)

20 pages, 407 KB

Open AccessArticle

The Association of Unhealthy Eating Behaviors with Sleep Quality Outcomes Among University Students: A Cross-Sectional Study

by Maha Al-Jawarneh, Shalini Chauhan, Ildikó Csölle and Szimonetta Lohner

Nutrients 2025, 17(22), 3580; https://doi.org/10.3390/nu17223580 (registering DOI) - 15 Nov 2025

Abstract

Background: This study examined the association between specific unhealthy eating behaviors and sleep quality among university students. Understanding how dietary habits affect sleep during significant lifestyle transitions associated with university experience can inform health promotion strategies. Methods: A cross-sectional design was [...] Read more.

Background: This study examined the association between specific unhealthy eating behaviors and sleep quality among university students. Understanding how dietary habits affect sleep during significant lifestyle transitions associated with university experience can inform health promotion strategies. Methods: A cross-sectional design was employed using a self-reported questionnaire to assess eating behaviors, timing of meals, and sleep-related behavior among a sample of international university students. Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI) tool. Statistical analyses were used to assess the relationship between eating patterns, overall sleep quality, and its components. Results: More than half of the students had poor sleep quality (51.7%). Daytime dysfunction was significantly more common in females than in males (27.9% vs. 8.3%, respectively; p < 0.001). Conversely, poor sleep efficiency was more prevalent among males than females (27.5% vs. 15.8%; p = 0.008). Multivariate logistic regression revealed that, compared to students who did not frequently consume heavy evening meals, those who did were more likely to experience poor sleep quality (OR = 2.73, 95% CI: 1.575–4.731). Similarly, those who frequently substitute snacks for main meals were more likely to experience poor sleep quality than those who did not (OR = 2.68, 95% CI: 1.465–4.895). Finally, students who ate within three hours of bedtime had higher odds of poor sleep quality compared to those who had their last meal more than three hours before bedtime (OR = 2.06, 95% CI: 1.173–3.629). Conclusions: Unhealthy dietary habits, such as consuming heavy evening meals, substituting snacks for main meals, and having a short meal-to-bedtime interval, were significantly associated with poor sleep quality. Interventions promoting healthier dietary patterns and appropriate meal timing could help improve sleep in this population. Full article

(This article belongs to the Section Nutrition and Public Health)

22 pages, 772 KB

Open AccessArticle

The Impact of Advertising Image Types on Consumer Purchasing Behavior of Fresh Agricultural Products

by Fan Huang, Yumeng Gu, Zhonghu Bai and Yani Dong

Foods 2025, 14(22), 3915; https://doi.org/10.3390/foods14223915 (registering DOI) - 15 Nov 2025

Abstract

Advertising images constitute an important factor influencing consumer purchase intentions in commercial settings. Drawing on the perspective of self-conscious emotions, this study examines the impact of advertising image types for fresh agricultural products on consumer purchase intentions and explores the underlying mechanisms. Advertising [...] Read more.

Advertising images constitute an important factor influencing consumer purchase intentions in commercial settings. Drawing on the perspective of self-conscious emotions, this study examines the impact of advertising image types for fresh agricultural products on consumer purchase intentions and explores the underlying mechanisms. Advertising images are classified into three categories: meat-typical, animal-typical, and composite. Evidence from two randomized experiments reveals the following findings: (a) The effectiveness of the three advertising image types in promoting purchase intentions follows the order of meat-typical > animal-typical > composite; (b) guilt mediates the relationship between advertising image types and purchase intentions, such that composite images evoke greater guilt than meat-typical and animal-typical images, thereby reducing consumer willingness to purchase; and (c) self-construal partially moderates the mediating effect of guilt, in that interdependent self-construal consumers exposed to composite advertising images are more likely to experience heightened guilt and consequently exhibit lower purchase intentions. This study extends the application of animal-related classifications in advertising and marketing research and provides new empirical evidence and practical insights for the design of advertising strategies for fresh agricultural products. Full article

(This article belongs to the Special Issue Consumer Behavior and Food Choice—4th Edition)

15 pages, 3606 KB

Open AccessArticle

Microfluidic Concentration Manipulation via Controllable AC Electroosmotic Flow

by Jingliang Lv, Yulong Pei and Jianqi Sun

Micromachines 2025, 16(11), 1288; https://doi.org/10.3390/mi16111288 (registering DOI) - 15 Nov 2025

Abstract

The ability to precisely prepare microfluids with targeted concentrations is critical for numerous applications, including protein crystallization and drug efficacy evaluation. This study presents an efficient microfluidic method for the continuous preparation of fluids at desired concentrations utilizing AC electroosmosis (ACEO). Two miscible [...] Read more.

The ability to precisely prepare microfluids with targeted concentrations is critical for numerous applications, including protein crystallization and drug efficacy evaluation. This study presents an efficient microfluidic method for the continuous preparation of fluids at desired concentrations utilizing AC electroosmosis (ACEO). Two miscible fluids of different initial concentrations are introduced through separate inlets. Target concentrations are achieved through ACEO-driven mixing, where fluid manipulation via electric signal and flow velocity control enables precise concentration adjustment at the outlet. To elucidate the concentration control mechanism via ACEO, we develop a three-dimensional numerical model coupling electric, flow, and concentration fields. Our results demonstrate that concentration modulation is significantly influenced by intrinsic fluid properties and external control parameters, including fluid viscosity, conductivity, axial fluid velocity, driving voltage, and signal frequency. Specifically, higher fluid viscosity and conductivity dampen electroosmotic flow, necessitating increased voltage to achieve target concentration. Axial fluid velocity determines the residence time in the mixing zone, directly affecting mixing efficiency and concentration control effect. The intensity of ACEO flow increases with applied voltage, enabling tunable mixing performance and outlet concentration. Overall, the simplicity of device design combined with precise concentration manipulation makes this method particularly attractive for applications requiring accurate fluid preparation. Full article

(This article belongs to the Special Issue Recent Development of Micro/Nanofluidic Devices, 2nd Edition)

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